• Thymus
  • These transgenic mice express neuronally derived human amyloid beta-precursor protein, APP gene, 770 isoform, containing the Swedish K670N/M671L, Dutch E693Q and Iowa D694N mutations, under the control of the mouse thymus cell antigen 1, theta, Thy1 , promoter. (jax.org)
  • A transgenic construct containing 2.1kb of the human amyloid beta-precursor protein, APP gene, 770 isoform, with the Swedish K670N/M671L, Dutch E693Q and Iowa D694N mutations, under the control of the mouse thymus cell antigen 1, theta, Thy1 , promoter was injected into fertilized C57BL/6 mouse eggs. (jax.org)
  • It was originally named theta (θ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). (wikipedia.org)
  • Analysis of Differencial glycosylation of Thy-1 from brain and thymus showed that all the complex N-linked structures differed between the two forms, superimposed upon a site specific common core. (wikipedia.org)
  • Antibody
  • The ELISA analytical biochemical technique of the MBS2883482 kit is based on THY1 antibody-THY1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect THY1 antigen targets in samples. (mybiosource.com)
  • Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. (mybiosource.com)
  • Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. (mybiosource.com)
  • Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (mybiosource.com)
  • It is the bound IgE antibody that confers a selective response of these cells to environmental substances, for example, pollen proteins or helminth antigens. (wikipedia.org)
  • membrane
  • MBS2883482 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thy-1 membrane glycoprotein (THY1) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Thy-1 is one of the most heavily glycosylated membrane proteins with a carbohydrate content up to 30% of its molecular mass. (wikipedia.org)
  • a plasma membrane component) and lysophosphatidic acid (LPA) use heterodimeric LPAR1-CD97 to drive Gi/o protein-phospholipase C-inositol 1,4,5-trisphosphate signaling and induce [Ca2+] in breast cancer cells. (wikipedia.org)
  • receptor
  • When the T cell receptor is engaged by the specific antigen presented by MHC, Lck acts to phosphorylate the intracellular chains of the CD3 and ζ-chains of the TCR complex, allowing another cytoplasmic tyrosine kinase called ZAP-70 to bind to them. (wikipedia.org)
  • This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. (wikipedia.org)
  • It functions through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling. (wikipedia.org)
  • Furthermore, ectopic CD97 expression facilitated RhoA activation through binding of Gα12/13 as well as induced Ki67 expression and phosphorylated ERK and Akt through enhancing lysophosphatidic acid receptor 1 (LPAR1) signaling. (wikipedia.org)
  • invariant
  • In this regard it has yet another historic association: Thy1 happens to be the first glycoprotein in which cell type specificity of variant glycosylation on an invariant protein was demonstrated. (wikipedia.org)
  • Immunoglobulin
  • Contains 1 Ig-like V-type (immunoglobulin-like) domain. (abcam.com)
  • Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. (wikipedia.org)
  • thymic
  • The composition of Thy-1 carbohydrate moieties varies considerably between different tissues or even among cells of the same lineage at different stages of differentiation: e.g., galactosamine only in brain Thy-1, sialic acid in thymic Thy-1 in far excess than brain Thy-1, that too increasing in parallel with T cell maturation. (wikipedia.org)
  • genes
  • The γ locus consists of three functional Jγ-Cγ genes and up to seven Vγ genes, with four of the Vγ genes clustered together upstream of Jγ1 - Cγ1 ( Fig. 1 B ) ( 2 - 4 ). (rupress.org)
  • lipid
  • His group also demonstrated that Thy1 was retained on the cell surface through a lipid anchor rather than via transmembrane domains, something which had not previously been appreciated but which is now known to be a feature of many receptors. (wikipedia.org)
  • Furthermore, γδ T cells are believed to have a prominent role in recognition of lipid antigens. (wikipedia.org)
  • Protein
  • The 25-kDa core protein (excluding the heavy glycosylation) of rodent Thy-1 is 111 or 112 amino acids in length, and is N-glycosylated at three sites (In contrast to only two glycosylation sites for human Thy-1). (wikipedia.org)
  • Achaete-scute homolog 1 is a protein that in humans is encoded by the ASCL1 gene. (wikipedia.org)
  • Gene
  • NCBI/Uniprot data below describe general gene information for THY1 . (mybiosource.com)
  • This study was conducted to determine whether elevated hydrostatic pressure alters mitochondrial structure, triggers release of the dynamin-related guanosine triphosphatase (GTPase) optic atrophy type 1 (OPA1) or cytochrome C from mitochondria, alters OPA1 gene expression, and can directly induce apoptotic cell death in cultured retinal ganglion cell (RGC)-5 cells. (nih.gov)
  • mice
  • The discovery of Thy-1 in mice and humans led to the subsequent discovery of many other T cell markers, which is very significant to the field of immunology since T cells (along with B cells) are the major cellular components of the adaptive immune response. (wikipedia.org)
  • Subsequent studies of mice genetically altered to be MASH-1 deficient revealed defective development of both sympathetic and parasympathetic ganglia, the two constituents of the ANS. (wikipedia.org)
  • tissue
  • Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. (mybiosource.com)
  • Publications] A.Ueda et al: 'Thy-1 antigen dendritic cell and rejection of composite tissue limb allografts in rats' Transplantation. (nii.ac.jp)
  • DETC-derived cytokines such as keratinocyte growth factor-1/2 and insulin-like growth factor-1 have also involved in regulating the processes of tissue repair, keratinocyte proliferation and survival, migration and recruitment of inflammatory cells. (springer.com)
  • Mouse
  • Directed against the Thy 1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in the mouse (positive to AKR strain, negative to CVA and BALB/c). (novusbio.com)
  • concentration
  • The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10 ng/ml in biological research samples containing THY1, with an estimated sensitivity of 0.089 ng/mL. (mybiosource.com)
  • The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve. (mybiosource.com)
  • subsequent
  • Because it was discovered subsequent to studies on its homolog in Drosophila, the Achaete-scute complex, it was originally named MASH-1 for mammalian achaete scute homolog-1. (wikipedia.org)
  • Molecular
  • CD48 and CD2 molecular coupling together with other interaction pairs of CD28 and CD80, TCR and peptide-MHC and LFA-1 and ICAM-1 contribute to the formation of an immunological synapse between a T cell and an antigen presenting cell. (wikipedia.org)
  • cells
  • The origin of CD4+ Thy-1 follicular T cells appears to be the Thy+ population in the PALS, as both sets commonly share identical V(D)J rearrangements. (rupress.org)
  • It has been estimated that TCRγδ + T cells exhibit substantially greater potential sequence diversity than TCRαβ or primary Ig ( 1 ). (rupress.org)
  • However, γδ T cells are peculiar in that they do not seem to require antigen processing and major-histocompatibility-complex (MHC) presentation of peptide epitopes, although some recognize MHC class Ib molecules. (wikipedia.org)
  • Basophils are the least common of the granulocytes, representing about 0.5 to 1% of circulating white blood cells. (wikipedia.org)