• lymphocytes
  • To investigate the requirement for CD2 expression in activation of T lymphocytes via the CD3-Ti antigen/MHC receptor complex, we produced and characterized a series of CD2- Jurkat variants. (rupress.org)
  • Assuming that the Jurkat cell line is representative of normal cycling human T lymphocytes, we conclude that the presence of the CD2 molecule on the plasma membrane is not in itself a requirement for an operational CD3-Ti-alpha/beta receptor. (rupress.org)
  • In order to identify a reliable method for producing adequate amounts of functional antitumor cytotoxic T lymphocytes with a potentially long in vivo lifespan, we tested the T-cell expansion efficiency of a new artificial antigen-presenting cell-based system. (haematologica.org)
  • Townsend A, Bodmer H. Antigen recognition by class I-restricted T lymphocytes. (pubmedcentralcanada.ca)
  • against CD 20 surface antigen of B lymphocytes, dornase-alpha DNAse, TNF-alpha, IL-2-diptheria toxin fusion protein that targets cells displaying a surface IL-2 receptor, TNFR-1gG fragment fusion protein, enbrel, laronidase, teriparatide and parathyroid hormone derivatives. (google.com)
  • CD56+CD3lymphocytes express CD40L after incubation with IL-2. (nih.gov)
  • Staining profiles of o.n. cultured lymphocytes, obtained as indicated in the Materials and Methods section, by using PE-labeled antiCD40L TRAP-1, FITC-labeled anti-CD3, or anti-CD56 mAbs in a classical double fluorescence assay. (nih.gov)
  • Splenic T and B lymphocytes were positively isolated using anti-CD3-coated magnetic beads or a pan B cell isolation kit. (nih.gov)
  • T lymphocyte subsets, and CD28, T cell receptor (TCR), cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) and programmed death-1 (PD-1) expression in purified splenic T lymphocytes were analyzed using flow cytometry, Western blotting and laser confocal microscopy. (nih.gov)
  • receptors
  • These mutants lack detectable surface CD2 as determined by indirect immunofluorescence, immunoprecipitation analysis, and specific radiolabeled antibody binding assay, but nevertheless, expressed normal numbers of CD3-Ti receptors. (rupress.org)
  • lymphocyte
  • In contrast, triggering of their CD3-Ti components resulted in the normal set of T lymphocyte-associated activation events, including phosphoinositide turnover, elevation in intracellular free calcium, early gene-induction events, and IL-2 production. (rupress.org)
  • By binding to such a cell via one arm, to a T lymphocyte via the other arm and to an antigen-presenting cell like a macrophage, a natural killer cell or a dendritic cell via the heavy chains, an immunological reaction against the cancer cell is triggered. (wikipedia.org)
  • T-lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex. (pubmedcentralcanada.ca)
  • Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. (pubmedcentralcanada.ca)
  • Yang SY, Chouaib S, Dupont B. A common pathway for T lymphocyte activation involving both the CD3-Ti complex and CD2 sheep erythrocyte receptor determinants. (pubmedcentralcanada.ca)
  • Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. (uniprot.org)
  • induces
  • Our first demonstration that crosslinkage of TCR with the CD2 antigen induces proliferation of normal human CD4+ T cells and CD8+ T cells, in addition to revealing a novel activation mechanism utilizable by the two major subsets of T cells, suggest that the CD2 antigen might be targeted for the regulation of antigen-specific T cell immunity (e.g., organ transplantation). (rupress.org)
  • Treatment with polyclonal antilymphocyte globulins induces depletion of circulating CD3+CD4+CD28− T-cells in transplant recipients.Prevalences of peripheral circulating CD3+CD4+CD28− T-cells in 16 age- and sex-matched healthy controls, 5 allograft recipients before and 6 hours after the application of ATG-F and 11 control patients before and 6 hours after organ transplantation. (nih.gov)
  • Activation of T cell antigen receptor (TCR) induces tyrosine phosphorylations that mediate the assembly of signaling protein complexes. (nih.gov)
  • CD28
  • A) Purified CD4+CD25+, CD4+CD25− CD45RBlow and CD4+CD25−CD45RBhigh T cells were stimulated with plate-bound anti-CD3 (10 μg/ml) and anti-CTLA-4 (10 μg/ml), soluble anti-CD28 (2 μg/ml), and IL-2 (20 U/ml). 24 h later, cells were incubated with either biotin-conjugated chicken anti-TGF-β1 or biotin-conjugated normal chicken Ig G, washed, and stained with Cy-Chrome™-conjugated streptavidin. (nih.gov)
  • B-E) 105 CD4+ CD25+, CD4+ CD25−CD45RBlow and CD4+ CD25−CD45RBhigh T cells were stimulated with plate-bound anti-CD3 (10 μg/ml) and anti-CTLA-4 (10 μg/ml), soluble anti-CD28 (2 μg/ml) and IL-2 (20 U/ml) in 100 μl culture for 72 h. (nih.gov)
  • In summary, in vivo depletion of peripheral CD3(+)CD4(+)CD28(-) T-cells by ATG-F in transplant recipients was paralleled in vitro by ATG-F induced apoptosis. (nih.gov)
  • In this small pilot study the prevalence of peripheral circulating CD3+CD4+CD28− T-cells was tested before and 6 hours after organ transplantation with and without application of ATG-F. Results of a blinded laboratory investigator showed that ATG-F treatment resulted in a total decrement of peripheral T-cells. (nih.gov)
  • proteins
  • We employed a novel method to immunoisolate plasma membrane subfragments that were highly concentrated in activated TCR-CD3 complexes and associated signaling proteins. (nih.gov)
  • The CD3γ, CD3δ, and CD3ε chains are highly related cell-surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain. (wikipedia.org)
  • EpCAM
  • It binds to antigens CD3 and EpCAM. (wikipedia.org)
  • Examples include catumaxomab (EpCAM / CD3), ertumaxomab (HER2/neu / CD3), FBTA05 (CD20 / CD3, proposed trade name Lymphomun)and TRBS07 (GD2 / CD3, proposed trade name Ektomab), drugs against various types of cancer. (wikipedia.org)
  • cytokines
  • Peripheral blood mononuclear cells (PBMC) were activated in vitro by anti-CD3, and cytokines were quantified in supernatants. (nih.gov)
  • functional
  • Expression of a functional CD3-Ti antigen/MHC receptor in the absence of surface CD2. (rupress.org)
  • Conclusions Our artificial antigen-presenting cells might represent an efficient tool to rapidly obtain a sufficient number of functional T cells for adoptive immunotherapy in patients with cancer. (haematologica.org)
  • transduction
  • Suthanthiran M. Oxidative mitogenesis: participation of CD2 antigen in the generation and/or transduction of obligatory accessory signals. (pubmedcentralcanada.ca)
  • This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. (wikipedia.org)
  • protein
  • B) Effect of cholesterol depletions on recovery of signaling protein in α-CD3 immunoisolates. (nih.gov)
  • Phosphorylation of the ITAM on CD3 renders the CD3 chain capable of binding an enzyme called ZAP70 (zeta associated protein), a kinase that is important in the signaling cascade of the T cell. (wikipedia.org)
  • intracellular
  • The intracellular tails of the CD3 molecules contain a single conserved motif known as an immunoreceptor tyrosine-based activation motif or ITAM for short, which is essential for the signaling capacity of the TCR. (wikipedia.org)
  • Transmembrane
  • Transmembrane signaling of normal human T cells was explored with mAbs directed at TCR, CD2, CD4, CD5, or CD8 antigens and highly purified CD4+ T cells and CD8+ T cells. (rupress.org)
  • Containing aspartate residues, the transmembrane region of the CD3 chains is negatively charged, a characteristic that allows these chains to associate with the positively charged TCR chains. (wikipedia.org)
  • cells
  • Results Our artificial antigen-presenting cells expanded both polyclonal T cells and MART-1-specific CD8 + T cells in a more efficient manner than the other systems. (haematologica.org)
  • Stimulation with artificial antigen-presenting cells allows for the generation of viable T cells displaying an immunophenotype consistent with in vivo potential for persistence, without increasing the frequency of regulatory T cells. (haematologica.org)
  • The starting specificity of anti MART-1 CD8 + T cells was preserved after stimulation with artificial antigen-presenting cells and it was statistically greater when compared to the activity of the same cells expanded with the other systems. (haematologica.org)
  • Finally, our artificial antigen-presenting cells proved to be suitable for large-scale application, minimizing the volume and the costs of T-cell expansion. (haematologica.org)
  • A novel model for antigen-dependent activation of normal human T cells. (rupress.org)
  • α-CD3 immunoisolation from cholesterol-depleted Jurkat cells. (nih.gov)
  • CD5 is a good immunohistochemical marker for T-cells, although not as sensitive as CD3. (wikipedia.org)
  • CD3 is initially expressed in the cytoplasm of pro-thymocytes, the stem cells from which T-cells arise in the thymus. (wikipedia.org)
  • The antigen is found bound to the membranes of all mature T-cells, and in virtually no other cell type, although it does appear to be present in small amounts in Purkinje cells. (wikipedia.org)
  • This high specificity, combined with the presence of CD3 at all stages of T-cell development, makes it a useful immunohistochemical marker for T-cells in tissue sections. (wikipedia.org)
  • The net effect is that this type of drug links T cells (via CD3) and monocytes/macrophages, natural killer cells, dendritic cells or other Fc receptor expressing cells to the tumor cells, leading to their destruction. (wikipedia.org)
  • Independently, rat hybridoma cells targeting the other antigen are produced. (wikipedia.org)
  • polyclonal
  • We also assessed the expression of CD40L on polyclonal NK effectors derived from peripheral blood of normal donors, by double staining with anti-CD40L mAb TRAP-1 and anti-CD3 or anti-CD56 mAb. (nih.gov)