Antigens centromere. Medical search. Frequent questions

Beads assay could detect more autoantibodies than ELISA, suggesting autoantibodies target to antigens with native conformation. (elsevierpure.com)
After somatic hypermutations were reverted, autoantibodies drastically decreased antigen reactivity. (elsevierpure.com)
Rodríguez-Bayona et al found autoantibodies to centromere passenger protein INCENP, a protein responsible for chromosomal segregation and mitosis regulation, in one patient with GLPLS. (medscape.com)
Autoantibodies against nuclear antigens were detected a mean (±SD) of 5.6 ± 4.7 years before the onset of symptoms and 8.7 ± 5.6 years before diagnosis in 63% of the individuals who subsequently developed SLE. (nih.gov)
Autoantibodies against nuclear antigens were detected in individuals who developed SLE several years before the onset of symptoms and diagnosis. (nih.gov)
Autoantibodies developing in breast cancer are not epiphenomena, but likely reflect an antigen-driven autoimmune response triggered by epitopes developing in the mammary gland during breast carcinogenesis. (biomedcentral.com)
Results further suggest significant promise for the development of panels of breast cancer-specific, premalignant-phase autoantibodies, as well as studies on the autoantibody response to tumor associated antigens in the pathogenesis of cancer. (biomedcentral.com)
In an original report, the AMA and ACA autoantibodies were demonstrated not presented any cross-reactivity between mitochondrial and centromere autoantigens [ 7 ]. (scientificarchives.com)
Testing for autoantibodies against centromeric antigens ( Figure 1 ) and the AMA may help to define the prognosis of both autoimmune disorders. (scientificarchives.com)
Human ANA screen ELISA kit is designed for screening in vitro quantitative concentrations of IgG autoantibodies to nuclear antigens (antinuclear antibodies, ANA screen) in human serum or plasma. (elisakits.co.uk)
Although at least 9 proteins are known to be associated with the centromere complex, CENP-B is normally considered to be the major centromere antigen, since autoantibodies to this protein were found to be present at high titres in all ACA sera while titres of antibodies directed at two other centromere antigens (CENP-A, CENP-C) were often much lower. (arodia.com)
Mono- or polytopic, complex autoimmune disease (see also autoantibodies ) with a predominantly polygenic disposition characterised by an incorrect elimination of extracellular antigens. (altmeyers.org)
Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer's instructions and evaluated primarily using their NOVA View system. (cdc.gov)

The reactivity of these antibodies and their revertants were investigated by ELISA and newly developed antigen-binding beads assay, which can detect conformational epitopes. (elsevierpure.com)
The target profiling of serum ACA from 269 patients with SS, systemic sclerosis (SSc), primary biliary cirrhosis (PBC) and healthy controls revealed that ACA-positive patients have antibodies against various sites of centromere complex regardless of disease. (elsevierpure.com)
We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
Specimen will be tested for antibodies to 13 nuclear antigens and the results compared to pre-established cutoffs for systemic autoimmune disease. (iclabs.ca)
We used indirect immunofluorescence, the crithidia assay for anti-dsDNA antibodies, and multiple ELISAs for extractable nuclear antigens. (biomedcentral.com)
Anti-centromere antibodies were mainly anti-CENP-B. ELISAs for extractable nuclear antigens and the assays for dsDNA were negative. (biomedcentral.com)
Tan EM, Rodman GP, Garcia 1, Moroi Y, Fritzler MJ, Peebles C. "Diversity of Antinuclear Antibodies in Progressive Systemic Sclerosis, Anti-Centromere Antibody and its Relationship to CREST Syndrome. (calbiotech.com)
Anticentromere antibodies (ACA) are considered an important diagnostic marker of scleroderma or systemic sclerosis (SSc), being CENP-B the major centromere auto-antigen recognized by sera from SSc patients [ 1 ]. (scientificarchives.com)
Thus, the co - appearance of anti-centromere antibodies with others in SSc patients at different stages of disease may suggest some putative significance for the evaluation and development of this disorder [ 14 ]. (scientificarchives.com)
Anti-centromere antibodies in a large cohort of systemic sclerosis patients: comparison between immunofluorescence, CENP-A and CENP-B ELISA. (scientificarchives.com)
They have a number of different subtypes which include anti-histone, anti-sp100, anti-centromere, anti-Scl-70, anti-Ro, anti-La, anti-nRNP, anti-Sm and anti-dsDNA antibodies. (elisakits.co.uk)
Subsequent studies confirmed that anti-centromere antibodies (ACA) were most often found in patients with a limited form of systemic sclerosis previously referred to as CREST syndrome. (arodia.com)
In addition, it was aimed to compare the results of indirect immunofluorescence (IIF) antinuclear antibody (ANA) patterns and extractable nuclear antigen (ENA) antibodies. (unboundmedicine.com)

Inner centromere protein is a protein that in humans is encoded by the INCENP gene. (wikipedia.org)
The inner centromere proteins (INCENPs), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. (wikipedia.org)
Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C". The Journal of Biological Chemistry. (wikipedia.org)
ACA recognises centromere â € complex' rather than individual protein, and this feature is common among patients with SS, SSc and PBC. (elsevierpure.com)
52 and 60 kDa), anti-Sjögren's syndrome antigen B, anti-Smith antibody, ribonucleoprotein, scleroderma, anti-histidyl-tRNA synthetase antibody, double-stranded DNA (dsDNA), centromere protein B and histones were analysed using the AtheNA Multi-Lyte ANA II Plus Test System on a Bio-Plex Array Reader (Luminex200). (nih.gov)
Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. (antibodies-online.com)
Type I partition systems, also known as ParABS include an ATPase motor protein, ParA, responsible for the movement of the replicated chromosomes to the distal pole of the cell, a DNA binding protein ParB, and a centromere-like DNA sequence, parS ( Funnell, 2016 ). (elifesciences.org)
Evaluation of an ELISA using recombinant fusion protein CENP-B as antigen. (scientificarchives.com)
Yoda, K., A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity. (riken.jp)
centromere protein E [Source:HGNC Symbol;A. (gsea-msigdb.org)
inner centromere protein [Source:HGNC Symb. (gsea-msigdb.org)
Description: A sandwich quantitative ELISA assay kit for detection of Human Centromere Protein F (CENPF) in samples from tissue homogenates, cell lysates or other biological fluids. (kits-elisa.com)
Description: A competitive ELISA for quantitative measurement of Human Centromere protein F(CENPF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (kits-elisa.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Centromere Protein F (CENPF) in Tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Centromere Protein F (CENPF) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
Bod1 depletion does not affect the activity or localization of Aurora B but does cause mislocalization of the microtubule depolymerase mitotic centromere- associated kinesin and prevents its efficient phosphorylation by Aurora B. Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles. (rupress.org)

Using new ELISA-based technology (sometimes called EIA for enzyme immunoassay) to screen for multiple extractable nuclear antigen (ENA) markers, centromere and double-stranded DNA (dsDNA) has automated testing at Baptist Medical Center and greatly improved the PPV for our ANA screen. (clinlabint.com)

This study aims to clarify overall of autoantibody production at lesion site, including anti-centromere antibody (ACA)-positive SS. (elsevierpure.com)
Conclusion We showed direct evidences of antigen-driven maturation of anti-SSA/SSB antibody and ACA in SS lesion. (elsevierpure.com)

Characteristics of a Soluble Nuclear Antigen Precipitation with Sera of Patients with Systemic Lupus Erythematosus. (calbiotech.com)
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. (lookformedical.com)
Fourteen of the patients (10.6%) with centromere, nuclear membrane (NM), multiple nuclear dot (MND) staining patterns and elevated liver enzymes could not be diagnosed as a specific disease and were followed up. (unboundmedicine.com)

These more specific antigen have the ability to react with single auto-antigens for example: Sm/RNP, SS-B/La, dsDNA, Scl-70, Sm, SS-A/Ro etc. (elisakits.co.uk)

The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle while its C-terminal domain is responsible for the interaction with Aurora B. In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins. (wikipedia.org)
The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle. (wikipedia.org)
We also did selective epitope mapping using BLAST and showed similarities and homology between spike, nucleoprotein, and many other SARS-CoV-2 proteins with the human tissue antigens mitochondria M2, F-actin and TPO. (frontiersin.org)
MHC-I and MHC-II proteins are key components of the antigen presentation machinery responsible for neoantigen presentation to CD8 + and CD4 + T lymphocytes, respectively. (portlandpress.com)

Mouse hepatitis virus utilizes two carcinoembryonic antigens as alternative receptors. (uci.edu)

An alternative view is that centromere identity is specified by an epigenetic mark on the chromosome that is independent of its underlying DNA ( Karpen and Allshire 1997 ). (rupress.org)
PNA FISH Centromere probes are recommended for tracking chromosome aberrations in the centromere such as trisomy and polysomy. (eurogentec.com)

The specification of metazoan centromeres does not depend strictly on centromeric DNA sequences, but also requires epigenetic factors. (rupress.org)
Masumoto, H., A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite. (riken.jp)

Cytological analysis of DNA replication further demonstrated that centromeres replicate asynchronously in parallel with numerous other genomic regions. (rupress.org)
Their expression in tumour cells is modulated by a complex interplay of genomic, transcriptomic and post translational factors involving multiple intracellular antigen processing pathways. (portlandpress.com)

Although some of these features resemble those in the rheumatic autoimmune diseases and primary biliary cirrhosis, the data suggest the involvement of an entirely different set of epithelial antigens in breast cancer. (biomedcentral.com)

In the present study, we aimed to study the association of human leukocyte antigen (HLA) with familial and non-familial SSc patients [limited cutaneous SSc (lcSSc) and diffuse cutaneous SSc (dcSSc)] from North India. (bvsalud.org)

This process is thought to be mediated by immune cells and no role is considered for the autoantibody response to tumor associated antigens [TAAs]. (biomedcentral.com)

The sequence of the Biotin-labeled centromere probe AAACTAGACAGAAGCATT is 5' labeled by the Biotin fluorescent Dye. (eurogentec.com)

ANA patterns were detected in 32.6% (43/132) of the patients (NM 9.1%, centromere 9.1%, MND 6.8%, respectively). (unboundmedicine.com)
In our study, 87.5% (70/80) of the patients diagnosed as PBS were found to have AMA positivity and 12.5% (10/80) of them had ANA positivity (such as NM, CNN, centromere). (unboundmedicine.com)

Quantitative fluorescence microscopy and transient transfection in the presence of aphidicolin, an inhibitor of DNA replication, show that CENP-A can assemble into centromeres in the absence of DNA replication. (rupress.org)

Similar to lichen planus, GLPLS is likely the result of a T-cell-mediated immune response of unknown etiology, which involves destruction of keratinocytes expressing specific antigens. (medscape.com)
HLA antigens are hypothesized to enhance a T-cell-mediated immune response of unknown etiology. (medscape.com)
A key mechanism of immune evasion deployed by tumour cells is to reduce neoantigen presentation through down-regulation of the antigen presentation machinery. (portlandpress.com)
This leads to a loss of tolerance and a consecutive pathological immune stimulation with specificity for the body's own antigens. (altmeyers.org)

CENP-B may therefore function as a trans-acting factor that regulates the formation or centromere-specific chromatin on the α-satellite DNA repeat at the nucleosome assembly level. (arodia.com)

Uncoupling DNA replication from CENP-A synthesis suggests that regulated chromatin assembly or remodeling could play a role in epigenetic centromere propagation. (rupress.org)

The second study examined 388 consecutive samples that were measured using HEp-2 IFA (1:160), an EIA screen and, if that were positive, single ENA differentiation antigens [10]. (clinlabint.com)

Systemic lupus erythematosus (SLE) is the prototypic systemic autoimmune rheumatic disease with autoantibodies against cellular (particularly nuclear) antigens, some of which are critically implicated in the autoimmune pathology while others provide valuable serodiagnostic markers for the disease. (biomedcentral.com)
Immune (or idiopathic) thrombocytopenic purpura (ITP), including Evans syndrome, is characterized by low platelet count and presence of autoantibodies to platelet surface antigens [ 5 - 7 ]. (omicsonline.org)
Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer's instructions and evaluated primarily using their NOVA View system. (cdc.gov)
Immune disorders exist when one's immune system begins generating potentially destructive antibodies to self antigens (autoantibodies). (frederickhealth.org)

During mitotic division, a transient structure called kinetochore is formed on top of the centromeres. (wikidoc.org)
A centromere functions in sister chromatid adhesion, kinetochore formation, and pairing of homologous chromosomes . (wikidoc.org)
2004). The presence of CENP-A is believed to be important for the assembly of the kinetochore on the centromere. (wikidoc.org)
Besides CENP-A, stable centromere recruitment of CENP-N requires a coincident interaction with a newly identified binding motif on nucleosome-bound CENP-C. Collectively, our studies clarify how CENP-N and CENP-C decode and stabilize the non-canonical CENP-A nucleosome to enforce epigenetic centromere specification and kinetochore assembly. (elifesciences.org)
In the absence of centromere, a kinetochore will not assemble on the The spindle is what moves the chromosomes during mitosis and meiosis. (netlify.app)

17. [Clinical analysis of anti-topoisomerase I antibody and anti-centromere antibody in diagnosis of collagen diseases]. (nih.gov)

A centromere is the region where sister chromatids join in the double chromosomal structure during mitosis , prophase and metaphase . (wikidoc.org)

The centromere is together with telomeres and origin of replications one of the essential parts of any eukaryotic chromosomes. (wikidoc.org)
When the centromere doesn't function properly, the chromosomes don't align and separate properly, resulting in the wrong number of chromosomes in the daughter cells ( aneuploidy ), and conditions such as Down syndrome , if the cells survive at all. (wikidoc.org)

11. IgG reactivity with a 100-kDa tissue and endothelial cell antigen identified as topoisomerase 1 distinguishes between limited and diffuse systemic sclerosis patients. (nih.gov)

The primary function of centromeres is to provide a platform for the assembly of macromolecular complexes known as kinetochores, whose task in turn is the physical capture of microtubules of the mitotic spindle. (elifesciences.org)
Kinetochores contain approximately 30 core subunits, normally subdivided in centromere-proximal and microtubule-proximal groups. (elifesciences.org)
Chromatids, centromeres, kinetochores. (netlify.app)

In this chromatin, the normal histone H3 is replaced with a centromere-specific variant, CENP-A in humans (Lodish et al. (wikidoc.org)

If spindle fibers have not attached to the centromeres the process stops here. (angelfire.com)

Here, we reveal the structural basis for the exquisite selectivity of CENP-N for centromeres. (elifesciences.org)

this gene encodes the production of a cell surface molecule called HY antigen. (scopeheal.com)
African-derived ``null'' variant of the Duffy Antigen Receptor for Chemokines (DARC) gene. (nih.gov)

Each consists of two CHROMATIDS or arms and are held together at a central body, the CENTROMERE. (angelfire.com)
Answer: The structure which maintains similar chromatids bound is the centromere. (tutorsglobe.com)

Epigenetic inheritance plays a major role in specifying the centromere in most organisms. (wikidoc.org)

The human leukocyte antigen (HLA) genomic region at chromosomal position 6p21 encodes many genes, which are important for immune system. (medscape.com)

HASPIN may act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment, and normal progression through the cell cycle. (antibodiesinc.com)

Patients with diffuse SSc showed mainly an anti-PM/Scl-75 response, whereas most cases of overlap syndromes were characterized by reactivity to both PM/Scl antigens. (biomedcentral.com)

Ki-67 Antigen" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)

Gutierrez-Caballero C, Cebollero LR, Pendas AM. Shugoshins: from protectors of cohesion to versatile adaptors at the centromere. (medlineplus.gov)

Among the genes located in the HLA class-II DQ-DP interval, eight genes are categorized into antigen processing/presentation-related genes, including TAP1 , TAP2 , LMP2 , LMP7 , HLA-DMA , HLA-DMB , HLA-DOA , and HLA-DOB . (medscape.com)
[ 10 , 18 ] Considering the importance of antigen presentation during immune response to HCV as well as what we have observed previously, we hypothesized that genomic variants of these genes may play a role in generating immune responses and contribute to the outcomes of HCV infection. (medscape.com)

A key function of the immune system is to distinguish foreign antigens (such as from infectious agents) from one's own tissues. (frederickhealth.org)

The detection of linkage between PGM-3 and the centromere using ovarian teratomas? (nih.gov)

Substitutions of the invariant arginine anchor residue in GAG result in global redistribution of PFV and macaque simian foamy virus (SFVmac) integration sites toward centromeres, dampening the resulting proviral expression without affecting the overall efficiency of integration. (figshare.com)

This provides the first clue to the possible orientation of the HLA region in relation to the centromere. (nih.gov)

This graph shows the total number of publications written about "Ki-67 Antigen" by people in this website by year, and whether "Ki-67 Antigen" was a major or minor topic of these publications. (umassmed.edu)

Anatomy33

Organisms9

Diseases6

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes65

Technology, Industry, Agriculture1

Information Science3

Health Care1

Autoantibodies13

Antibodies16

Protein16

Extractable Nuclear1

Antibody2

Nuclear3

DsDNA1

Proteins4

Carcinoembryonic1

Chromosome2

Centromeric2

Genomic2

Autoimmune1

Human1

Autoantibody1

Sequence1

Patients2

Quantitative1

Immune4

Specific1

Role1

Study1

Autoantibodies4

Kinetochore5

Anti-centromere1

Mitosis1

Chromosomes2

Endothelial cell1

Kinetochores3

Chromatin1

Spindle fibers1

CENP1

Gene2

Chromatids2

Epigenetic1

Encodes1

Metaphase1

Reactivity1

MeSH1

Cohesion1

Genes2

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Major1