RecombinantMonoclonal AntibodyAntigenCollagen and elastinLoss of the elastin layerAnti-elastin antibodyProteinELISA KitPolyclonal and monoclonal antibodiesInvolving elastin degradationReactivityDegradationOcular pathologyEnzymesSpecificityDouble AntibodyThermo Fisher SciPeptideProteinsDatasheetDepositionFibrillinPolypeptidesElasticGeneImmune systemFibersCollagensSerumSynthesisComplement activationMiceTropoelastinRecoilProductsTurnoverMoleculesElasticityThyroidAssessWestern BlotStructuralSpeciesDamage
Recombinant4
- Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
- Compared with the polyclonal and monoclonal antibodies of MME, this MME recombinant antibody has the features of increased reproducibility and control, animal-free technology, high degree of monovalency, high batch-to-batch consistency, easier isotype conversion, etc. (cusabio.com)
- Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
- Figure-1 : ELISA binding of PRTN3 Antibody (Clone: 11F1A5) with Human PRTN3 recombinant protein, Coating antigen: PRTN3 at 1 µg/ml, PRTN3 antibody dilutions start from 1000 ng/ml, EC50= 9.667 ng/ml. (abeomics.com)
Monoclonal Antibody1
- The following product was used in this experiment: Fibulin 5 Monoclonal Antibody (3F8D5,7F8D5) from Thermo Fisher Scientific, catalog # MA5-15293, RRID AB_10980961. (thermofisher.com)
Antigen5
- Antibodies with Advanced Verification data have been validated for specificity to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- To determine the circulating antibody to self-antigen response, a modified enzyme-linked immunosorbent assay was performed against antibodies to collagen type V and elastin fragments.ResultsPeripheral blood was isolated from 266 patients without diabetes mellitus (n=182), with diabetes mellitus not treated with metformin (n=34), and with diabetes mellitus actively taking metformin (n=50) from 2015 to 2017. (deepdyve.com)
- Lastly, no differences in expression of collagen type V and elastin fragment antigen and/or antibodies were detected with metformin use in diabetics.ConclusionMetformin in diabetics at-risk for abdominal aortic aneurysm or diagnosed with abdominal aortic aneurysm does not seem to alter the peripheral inflammatory environment. (deepdyve.com)
- Results: Elimination of Fcγ receptors, preventing antigen/antibody-dependent cytotoxicity, protected against SIOP. (marclab.org)
- Basic Serology Panel #1 RPR Syphilis Serology, ABO-Rh Typing HIV-1/HIV2 Antibody Screen, Hepatitis B Surface Antigen, Hepatitis B Core IgG/Igm Antibody Hepatitis C Virus Antibody, HTLV-I Antibody. (elastin.com)
Collagen and elastin4
- The dermis contains mainly collagen and elastin. (skintypesolutions.com)
- As we age, collagen and elastin both decline. (skintypesolutions.com)
- We have been examining the mechanisms of collagen and elastin turnover in pulmonary fibrosis and related diseases. (vumc.org)
- Structural proteins, such as collagen and elastin, provide support to tissues and organs. (atlasbars.com)
Loss of the elastin layer2
- In aged and AMD eyes, loss of the elastin layer (EL) of Bruch's membrane (BrM) has been reported. (utah.edu)
- As loss of the elastin layer of Bruch's membrane (BrM) has been reported in aging and AMD, we previously showed that immunization with elastin peptide oxidatively modified by cigarette smoke (ox-elastin), exacerbated ocular pathology in the smoke-induced ocular pathology (SIOP) model. (marclab.org)
Anti-elastin antibody2
- A decreased level of anti-elastin antibody was reported to be associated with emphysema and more severe disease (22). (massorrerjazzbar.com)
- One 96-Well Plate: Pre-coated with anti-elastin antibody. (elisakits.co.uk)
Protein9
- The nanoparticles in Vyavahare's research are coated with an elastin antibody, causing them to attach to the degraded elastin, a protein in the aneurysm. (nanowerk.com)
- Elastin is a protein that gives skin its snap and elasticity. (skintypesolutions.com)
- Oxytalan fibers contain the microfibrillar protein fibrillin but no elastin. (skintypesolutions.com)
- Elastin is a highly insoluble matrix protein with very low turnover. (vumc.org)
- cDNA and antibody probes are used, respectively, to study regulation of elastin mRNA and protein synthesis, particularly related to modulation of elastin metabolism in diseases of human connective tissues such as blood vessels, lung and skin. (vumc.org)
- Sakai et al identified fibrillin, a 350-kd protein, by using monoclonal antibodies raised against myofibrils. (medscape.com)
- One important form of such damage in aging tissues is the fraying and breakage of structures made of the stretchy protein elastin . (sens.org)
- Elastin (ELN) is a protein found in the matrix, for providing elasticity and resilience to various tissues like the skin, lungs, and blood vessels. (elisakits.co.uk)
- Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
ELISA Kit2
- Human elastin ELISA kit can measure concentrations of ELN (elastin) present is plasma, serum, biological fluids or tissue homogenate samples. (elisakits.co.uk)
- For this elastin ELISA kit, it is recommended that a standard curve is generated for each assay carried out. (elisakits.co.uk)
Polyclonal and monoclonal antibodies1
- Our beta Galactosidase polyclonal and monoclonal antibodies are developed in Rabbit, Mouse and Chicken. (thermofisher.com)
Involving elastin degradation1
- Vyavahare's work has also raised hopes that nanoparticles could be used to treat other ailments involving elastin degradation, including chronic obstructive pulmonary disease, commonly called COPD. (nanowerk.com)
Reactivity1
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Lysyl Oxidase (LOX) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (antibody-tech.com)
Degradation2
- Inflammatory conditions in aneurysms lead to elastin degradation. (nanowerk.com)
- Loss of CRP2 attenuated Ang II-induced AAA incidence and severity, accompanied by preserved smooth muscle α-actin expression and reduced elastin degradation, matrix metalloproteinase 2 (MMP2) activity, deposition of collagen, particularly collagen III (Col III), aortic tensile strength, and blood pressure. (biomedcentral.com)
Ocular pathology3
- Here we assess the role of elastin antibodies using a mouse model of smoke-induced ocular pathology (SIOP), which similarly demonstrates EL loss. (utah.edu)
- These data demonstrate that SIOP mice generate elastin-specific antibodies and that immunization with ox-elastin exacerbates ocular pathology. (utah.edu)
- Conclusions: These data further support ox-elastin role in ocular damage in in part via elastin-specific antibodies, and support the corollary that PIT with ox-elastin attenuates ocular pathology. (marclab.org)
Enzymes3
- Other drivers of damage to aging elastin include free radical attack, enzymes that degrade elastin in a failed attempt to remodel the tissue, and (in the skin) UV radiation from the sun. (sens.org)
- Proteins are essential for the growth and repair of tissues, the formation of enzymes, hormones, and antibodies, and the production of energy. (atlasbars.com)
- They play a catalytic role (enzymes), a mechanical role (elastin and collagen), a transportation and storage role (hemoglobin, myoglobin, cytochrome), and a protective role (antibodies). (ocati.com)
Specificity1
- These antibodies have been verified by Knockdown and Relative expression to confirm specificity to beta Galactosidase. (thermofisher.com)
Double Antibody1
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Lysyl Oxidase (LOX) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (antibody-tech.com)
Thermo Fisher Sci1
- The following product was used in this experiment: Fibulin 5 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-81899, RRID AB_2789060. (thermofisher.cn)
Peptide5
- C57BL/6J mice were immunized with elastin or elastin peptide oxidatively modified by cigarette smoke (ox-elastin). (utah.edu)
- We have a new collaborative manuscript out in iOVS, Peptide-Based Immunotherapy Against Oxidized Elastin Ameliorates Pathology In Mouse Model Of Smoke-Induced Ocular Injury. (marclab.org)
- Here we asked whether ox-elastin peptide-based immunotherapy (PIT) ameliorates damage. (marclab.org)
- Methods: C57BL/6J mice were injected with ox-elastin peptide at two doses via weekly subcutaneous administration, while exposed to cigarette smoke for 6 months. (marclab.org)
- These peptide usually contains the epitope recognized by the antibody. (affbiotech.com)
Proteins3
- In addition to antibodies, proteins called cytokines help to regulate the immune response and coordinate the body's defense against infections and diseases. (atlasbars.com)
- For example, antibodies are proteins that recognize and bind to specific carbohydrates on the surface of pathogens, helping to neutralize them. (atlasbars.com)
- We conclude that whether peptidoglycan binding proteins do not appear to be major adhesins, most of the choline-binding proteins interact with host proteins (elastin and C reactive proteins are the major Cbp partners). (biomedcentral.com)
Datasheet1
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
Deposition3
- Visual function was assessed by optokinetic response, retinal morphology by spectral-domain optical coherence tomography and electron microscopy, and complement activation and antibody deposition by Western blot. (utah.edu)
- Retinal morphology was assessed by by electron microscopy, and complement activation, antibody deposition and mechanisms of immunological tolerance were assessed by Western blotting and ELISA. (marclab.org)
- Mice receiving PIT with low dose ox-elastin (LD-PIT) exhibited reduced humoral immunity, reduced complement activation and IgG/IgM deposition in the RPE/choroid, and largely a preserved BrM. (marclab.org)
Fibrillin2
- The deeper reticular dermis houses thicker elastic fibers composed of both elastin and fibrillin called elaunin fibers running parallel to the surface. (skintypesolutions.com)
- Fibrillin 1 Antibody detects endogenous levels of total Fibrillin 1. (affbiotech.com)
Polypeptides2
- Human elastin-like polypeptides as a versatile platform for exploitation of ultrasensitive bilirubin detection by UnaG. (elisakits.co.uk)
- Polymorphisms in the human tropoelastin gene modify in vitro self-assembly and mechanical properties of elastin-like polypeptides. (elisakits.co.uk)
Elastic7
- Vyavahare described elastin as the "rubber band of life" and said it's the body's version of elastic material, making it possible for arteries to push blood forward. (nanowerk.com)
- Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. (thermofisher.com)
- Elastin is a major component of elastic fibers in the skin. (skintypesolutions.com)
- The elastin and elastic fibers made in youth have to last a lifetime. (skintypesolutions.com)
- Verhoeff Van-Gieson (EVG) elastin stain showed absent and fragmented elastic fibers in the dermis, and a Masson's trichrome stain demonstrated no abnormalities in collagen or smooth muscle ( Figures 4A and 4B ). (jcadonline.com)
- Whereas collagen fibrils accommodate the pulse flowing through the blood vessels by spreading outward away from each other, elastin stretches forward and snaps back into place like an elastic band. (sens.org)
- But just as an elastic band that is stretched and relaxed over and over again will gradually lose its recoil and eventually snap, so too individual fibrils of elastin in the arteries slowly wear out after decades of repeated, cyclical rounds of stretching and relaxing in response to each heartbeat, eventually causing them to fray and tear. (sens.org)
Gene2
- We study elastin gene expression in several biological systems. (vumc.org)
- Elastin gene on chromosome 7q11.2 encodes ELN. (elisakits.co.uk)
Immune system2
- Alanine strengthens the immune system by producing antibodies, and helps in the metabolism of sugars and organic acids. (thewellnessadvocate.com)
- In response to a bite from such a tick, a person's immune system might produce a specific antibody to recognize alpha-gal called IgE. (thechronicpainchronicle.com)
Fibers2
- The important role water plays is why moisturizing and hydrating the skin is important to help prevent damage of elastin fibers. (skintypesolutions.com)
- The expression of elastin and the formation of fibers reach their peak during fetal development and early postnatal development. (elisakits.co.uk)
Collagens1
- The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. (joplink.net)
Serum2
- Serum testing for antinuclear antibodies, thyroid autoantibodies, thyroid function, antiphospholipid antibodies (lupus anticoagulant and anticardiolipin antibody), and complement levels were all within normal limits. (jcadonline.com)
- reported increased levels of serum autoantibodies against collagen I, collagen II, collagen IV, elastin, aggrecan and cytochrome C (21). (massorrerjazzbar.com)
Synthesis2
- Abnormally low antibody markers of elastin synthesis in patients with firbormyalgia syndrome. (llu.edu)
- New elastin synthesis shuts down after puberty. (skintypesolutions.com)
Complement activation2
- Elastin antibodies represented complement fixing isotypes that, together with the increased presence of complement activation seen in immunized mice, suggest that elastin antibodies exert pathogenic effects through mediating complement activation. (utah.edu)
- Overall, damage is associated with complement activation, antibody-dependent cell-mediated cytotoxicity, and altered cytokine signature. (marclab.org)
Mice4
- Ox-elastin IgG and IgM antibodies were elevated in ox-elastin immunized mice following 6 months of smoke, whereas elastin immunization had a smaller effect. (utah.edu)
- Ox-elastin immunization exacerbated smoke-induced vision loss, with thicker BrM and more damaged retinal pigment epithelium (RPE) mitochondria compared with mice immunized with elastin or nonimmunized controls. (utah.edu)
- Following a 2-week period of rest, mice were challenged intratracheally with elastin for 3â days or 1â month. (bvsalud.org)
- Rag1-/- , Mmp12-/- , and Il17a-/- mice and neutralising antibodies against active elastin fragments were used for mechanistic investigations. (bvsalud.org)
Tropoelastin1
- Cells such as fibroblasts, smooth muscle cells and chondroblasts synthesize and secrete tropoelastin, which is the precursor of elastin. (elisakits.co.uk)
Recoil2
- Elastin provides firmness and recoil. (skintypesolutions.com)
- Elastin has a coiled structure that allows it to stretch and recoil. (skintypesolutions.com)
Products4
- Elastin Products Company, Inc. (elastin.com)
- As an Elastin Products Company, Inc. client, now you can enjoy the simple, fast and secure method of ordering your products at anytime during the day or night at your own convenience utilizing the Internet. (elastin.com)
- All items listed herein are manufactured by processes designed by Elastin Products Company, Inc., by cited literature methods with modifications or as custom preparations for EPC by qualified scientists. (elastin.com)
- Elastin Products Company, Inc. (EPC) warrants that its products are merchantable quality and warrants the accuracy of its description of such products. (elastin.com)
Turnover1
- Elastin has extremely low turnover in adult skin. (skintypesolutions.com)
Molecules1
- Water also plays a role, allowing the coiled elastin molecules to extend and contract. (skintypesolutions.com)
Elasticity1
- Elastin is abundant in tissues that require elasticity for functions including large arteries, lungs, skin, and ligaments. (elisakits.co.uk)
Thyroid1
- These auto-antibodies primarily attack the thyroid and gut, but they can also destroy the heart and other organs. (perfecthealthdiet.com)
Assess1
- Human GVAPGVGVAPGV/HLA-A*02:01 tetramer was synthesised to assess the presence of elastin-specific T cells in patients with COPD.We observed that 2â weeks of cigarette smoke exposure induced an elastin-specific T cell response that led to neutrophilic airway inflammation and mucus hyperproduction following elastin recall challenge. (bvsalud.org)
Western Blot3
- Antibodies that detect beta Galactosidase can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA and Immunoprecipitation. (thermofisher.com)
- All of our anti-MMP antibodies are produced and validated in-house to ensure they will meet your microscopy, ELISA, and western blot needs. (southernbiotech.com)
- Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. (ptglab.com)
Structural1
- FBN1 Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. (affbiotech.com)
Species1
- If you cannot find the antibody you're looking for, contact us today to develop custom antibodies for specific targets, species and applications. (thermofisher.com)
Damage1
- They would go only to the degraded elastin, allowing doctors to get vital information about the extent of damage. (nanowerk.com)