Antibodies glycopeptides. Medical search. Frequent questions

The glycan role in the glycopeptide immunogenicity revealed by atomistic simulations and spectroscopic experiments on the multiple sclerosis biomarker CSF114(Glc). (korilog.com)
Investigate binding profiles of potential glycan-binding samples (antibodies, proteins, or microbes). (zbiotech.com)
Our patented microarray substrate technology enables us to create glycan/glycopeptide/protein microarrays with custom layouts and dimensions. (zbiotech.com)
We came to Z-biotech for help characterising antibodies we produced against glycan targets. (zbiotech.com)
I would highly recommend using them for the analysis of glycan binding antibodies. (zbiotech.com)
1,2 discussed a new glycopeptide instant expertise method which makes use of multiple collision energies to produce glycan fragments and peptide fragments inside the same MS/MS spectrum. (news-medical.net)
Until recently, classification of N-glycopeptide spectra in ProteinScape (detection of glycopeptide spectra and calculation of glycan and peptide moiety mass) has centered on glycan fragment distances and oxonium ion detection when using typical CID conditions on ion trap or qTof instruments (see application note LCMS-66)4. (news-medical.net)
1,2 employed synthetic, and therefore well-defined, N-glycopeptides for the optimization of CID energy parameters, so as to attain the greatest possible quantities of data on both the glycan and peptide moiety within just one tandem MS experiment. (news-medical.net)
In this study neoglycoconjugates were fabricated and carbohydrates synthesized to investigate the antigenicity to anti-glycan antibodies. (srcinhibitors.com)
Both technologies are characterized by flow assay dynamics narrowing glycan-antibody interactions more closely to an in vivo environment. (srcinhibitors.com)
GAS also secretes proteases that cleave and degrade IgG antibodies, as well as a glycan hydrolase called EndoS, which deglycosylates IgG. (newsstatix.com)
Hypotheses: Messenger RNA vaccination of humanized mice, Rhesus macaques and humans will induce long-lasting anti-V3 glycan bnAb epitope antibodies, and mRNA vaccination will promote sequential somatic hypermutations and affinity maturation in V3-glycan targeted B cell lineages. (grantome.com)
This IPCAVD Program has the potential to change the field with a successful first in man Phase I clinical trial by showing the plausibility of initiation of V3-glycan bnAb B cell lineages with Man9-V3 glycopeptide primes and sequential nucleoside-modified mRNA boosts. (grantome.com)
This mode of cell-to-cell transmission is broadly susceptible to the activity of CD4 binding site (CD4bs) and glycan or glycopeptide epitope-specific broadly neutralizing monoclonal antibodies (bNMAbs) but shows resistance to bNMAbs targeting the Env gp41 subunit membrane-proximal external region (MPER). (ox.ac.uk)

Alemtuzumab, an tam antimicrobial drugs and trimethoprim-sulfamethoxa- anti-CD52 monoclonal antibody, causes lymphocytopenia zole and susceptible to aminoglycosides, tetracyclines, fl u- by eliminating CD52-positive cells. (cdc.gov)
ii) To develop combination therapy with monoclonal antibody (mAb) cocktails for disseminated candidiasis. (lsuhsc.edu)
On this conformation CSF114(Glc) binds the monoclonal antibody mAb8-18C5 equally to the myelin oligodendrocyte glycoprotein MOG, which is a identified MS auto-antigen, thus explaining its diagnostic exercise. (korilog.com)
Studies employed the avidin-biotin complex (ABC) immunohistochemical procedure and utilized rabbit polyclonal antibodies to arginine vasopressin (VP), provasopressin (ProVP), vasopressin-associated human glycopeptide (VAG), oxytocin (OT), oxytocin-associated human neurophysin (OT-HNP), and a mouse monoclonal antibody to vasopressin-associated human neurophysin (VP-HNP). (johnshopkins.edu)

Moreover, our lab has developed techniques to detect glycoproteins and glycopeptides across digestion. (oregonstate.edu)
Glycoproteins are sometimes acknowledged as not-self molecules by antibodies triggering the onset of extreme autoimmune illnesses resembling A number of Sclerosis (MS). Thus, the event of antigen-mimicking biomarkers represents a sexy technique for an early analysis of the illness. (korilog.com)
Our current glycopeptide microarray platform focuses on some of the most studied glycoproteins - MUC1 and SARS-CoV-2 spike protein. (zbiotech.com)
We identified a total of 587 glycoproteins, including 1239 glycopeptides, 526 monosaccharide components, and 8326 intact glycopeptides in glycoproteomics, as well as a total of 4496 proteins identified in proteomic analysis. (frontiersin.org)
This process allows the virus to avoid neutralization by a class of antibody targeting the gp41 subunit of the envelope glycoproteins. (ox.ac.uk)

British Library EThOS: Structural features of the MUC1 antigen and associated antibodies. (bl.uk)
After affinity isolation on immobilized BC3 antibody, MUC1 was partially deglycosylated by enzymatic treatment with alpha-sialidase/beta-galactosidase and fragmented by proteolytic cleavage with the Arg-C-specific endopeptidase clostripain. (nih.gov)
MUC1 glycopeptide array includes 62 MUC1 glycopeptides and 1 unglycosylated peptide, which allows researchers to detect and validate protein interactions with various glycoforms of MUC1 peptides. (zbiotech.com)
Another platform utilizes an identified cancer-specific immunodominant glycopeptide epitope in MUC1 [64], a heavily glycosylated mucin known to be Inhibitors,research,lifescience,medical associated to several cancer types including breast and ovarian cancer. (srcinhibitors.com)
A synthesized MUC1 peptide was also used as a carrier for the chemoenzymatic Inhibitors,research,lifescience,medical synthesis of glycoconjugates (O-glycopeptides), on NHS-activated glass slides via amine group guaranteeing covalent and site-specific VE-822 datasheet attachment [23,25,63]. (srcinhibitors.com)
In the current study we show that designer glycopeptides containing the Thomsen-Freidenreich (TF) antigen (β-Gal-[1→3]-α-GalNAc- O -serine) are immunogenic in vivo and generate TF-specific CTL capable of recognizing a variety of tumor cells in vitro including a MUC1-expressing tumor. (silverchair.com)

Glycopeptide 1 was used for the development of polyclonal antibodies and recombinant monoclonal antibodies by Phage Display technology. (cnr.it)
Accordingly, polyclonal antibodies were reactive to glycopeptide 1, which enables the application of anti-glycopeptide 1 antibodies in immune reactive assays targeting hypoglycosylated alpha-DG. (cnr.it)

In this context, this work describes the synthesis of the hypoglycosylated alpha-DG mimetic glycopeptide NHAc-Gly-Pro-Thr-Val-Thr[alphaMan]-Ile-Arg-Gly-BSA (1) as a potential tool for the development of novel antibodies applicable to dystroglycanopathies diagnosis. (cnr.it)
Using the convergent glycopeptide synthesis it is possible to synthesize libraries of homogeneously glycosylated peptides. (uni-bayreuth.de)
We offer tailored custom synthesis services for complex oligosaccharides, glycopeptides, glycoconjugates and glyconanoparticles from mg to multigram scale for applications ranging from novel biomarker development to preclinical studies. (aspariaglycomics.com)
If you are developing novel glycoproteomics workflows or looking into quantifying novel glycopeptide biomarkers ask for our custom Glycopeptide synthesis service with optional stable isotope labeling. (aspariaglycomics.com)
Examples of past projects include the synthesis of N-glycans, O-glycans, glycopeptides, glycosaminoglycans, GPI-anchors milk oligosaccharides and glycoconjugates. (aspariaglycomics.com)

Currently, analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) of intact glycopeptides is often the method of choice in site-specific glycoproteomic studies ( 12 ). (frontiersin.org)
We achieved site-specific glycosylation profiling for total of 183, 169 and 164 intact glycopeptides in HCC, LC, and HBV serum samples, respectively from Asn184, Asn207, Asn211 and Asn241 sites. (ntnu.edu.tw)

We defined six T-cell peptides found in Candida albicans cell wall proteins and conjugated each peptide, originally intended to act as a carrier to the fungal cell wall β-1,2- trisaccharide [β-(Man) 3 ] to create glycopeptide conjugate vaccines. (lsuhsc.edu)
Despite the fact that the classification workflow in ProteinScape has an explicit parameter to address this issue, the peptide mass of low abundant N-glycopeptides can be miscalculated at times, which can result in no or incorrect identifications. (news-medical.net)
In the case of N-glycopeptide fragmentation, the higher collision energies, which are required for peptide moiety fragmentation, also bring about a characteristic fragmentation pattern, which has earlier been described for MALDI fragmentation 3 . (news-medical.net)

Furthermore, a combination cocktail of monoclonal antibodies (mAbs) specific for the synthetic peptides of the double chimeric vaccine provided the most effective protection against disseminated candidiasis. (lsuhsc.edu)
Beyond peptides, our lab also examines the digestion of milk proteins, particularly immunoglobulins (antibodies) using proteomics, ELISA and functional assays. (oregonstate.edu)

Antibody to hepatitis B core antigen. (cdc.gov)
In conclusion the hyperglucosylated adhesin is the first example of an N-glucosylated native antigen that can be considered a relevant candidate for triggering pathogenic antibodies in MS. (unifi.it)
It is so rare to find the T antigen in healthy tissue, that we actually have antibodies against it. (dadamo.com)

This represents a target for selective recognition through broadly neutralizing, monoclonal antibodies (PG9). (uni-bayreuth.de)
A key goal of HIV-1 vaccine development is to induce long-lasting broadly neutralizing antibodies (bnAbs) that can inhibit HIV-1 infection. (grantome.com)

Any variety of glycopeptide sample can be employed in this workflow - low complex and high complex, with and without glycopeptide enrichment. (news-medical.net)
The first strategy uses antibody-conjugated magnetic nanoprobes for Hp enrichment from human serum, followed by hydrophilic interaction liquid chromatography for glycopeptide enrichment and identification by mass spectrometry (NP-HILIC-MS). This strategy was employed for Hp profiling of serum from 33 individual patients: 11 HCC patients, 11 liver cirrhosis patients (LC) and 11 Hepatitis B (HBV) inactive carriers. (ntnu.edu.tw)
In the second part, we developed a novel method to improve sensitivity for detecting specific glycopeptides: we developed a One Pot enrichment strategy for concomitant purification and glycosylation profiling of biomarker candidates in serum. (ntnu.edu.tw)
This approach takes advantages of dual nanoprobes: hemoglobin-conjugated silica nanoparticle (Hb@SiO2) for enrichment of target protein and ligand-conjugated magnetic nanoparticle (ligand@MNP) for enrichment of target glycopeptide. (ntnu.edu.tw)

As there is no approved antifungal vaccine for use in humans and significant therapeutic challenges remain, our approach is disease prevention through active vaccination and/or passive immunization with protective antibodies. (lsuhsc.edu)
These results demonstrate that protective antibodies to schistosome infections in brown rats and rhesus monkeys include IgG responses to the core Xyl/Fuc epitopes in surface-expressed N-glycans, and raise the potential of novel glyco-based vaccines that might be developed to combat this disease. (cdc.gov)

The PAP20 glycopeptides were isolated by reversed phase high pressure liquid chromatography and subjected to the structural analyses by quadrupole time-of-flight electrospray ionization mass spectrometry and to the sequencing by Edman degradation. (nih.gov)
For glycopeptide characterization, mass spectrometry has arisen as the go-to methodology. (news-medical.net)
By using mass spectrometry approaches, the researchers were able to determine site-specific differences in the IgG glycopeptide. (newsstatix.com)

Analyze the glycosylation of any biological samples (antibodies, proteins, or microbes). (zbiotech.com)
Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates , however they are not considered lectins . (lookformedical.com)
The portion of the matrix 2 (M2) protein that is outside the viral envelope … MAJOR IMMUNOGENIC PROTEINS Hemagglutinin is definitely a glycopeptide indicated on the surface of influenza viruses that facilitates access of the computer virus into sponsor cells.25 It is so named because of its property of agglutinating red blood cells. (ap26113.com)
However, Western blot analysis for all 12 fresh-frozen tumor samples showed the presence of two proteins, 42,000 and 20,000 daltons, that were immunoreactive with antibodies to VP, VP-HNP, and VAG. (johnshopkins.edu)

All humans normally possess antibodies that react with the Thomsen-Friedenreich (T) and the Tn antigens. (dadamo.com)
Not surprisingly, blood type A individuals have the least aggressive antibody immune response against the T and probably Tn antigens. (dadamo.com)
So the hypothesis goes that because of the lower level of antibody against T and Tn antigens or stumps, and because of this tendency for the immune system of A's to be a bit confused or disinclined to attack Tn antigens, blood type A is at an immunologic disadvantage in attacking any cell bearing these T and Tn antigenic markers. (dadamo.com)
Because of these encouraging results, we applied the same antigenic strategy to TACA-containing glycopeptides because induction of a degenerate T cell response against this class of tumor antigens may be of great advantage in cancer immunotherapy ( 10 - 12 ). (silverchair.com)

Past its intracellular function in virus replication, extracellular types of NS1 perform in immune modulation and are acknowledged by host-derived antibodies. (glycodigest.org)
They discovered that GAS bacteria can alter the structure and function of IgG to evade antibody-mediated immune responses. (newsstatix.com)
Everyone has preexisting anti-T and anti-Tn antibodies, or a built in immune system response against cells with these markers. (dadamo.com)
IMPORTANCE The ability of HIV-1 to move directly between contacting immune cells allows efficient viral dissemination with the potential to evade antibody attack. (ox.ac.uk)

Envelope protein-targeted vaccines for flaviviruses are restricted by issues of antibody-dependent enhancement (ADE) of infections. (glycodigest.org)
TACA-containing glycopeptides are appealing cytotoxic T cell (CTL)-based vaccines to prevent or treat cancer because the same sugar moieties are expressed in a variety of tumors, rendering a vaccination strategy applicable in a large population. (silverchair.com)
These results have implications for viral spread in vivo and the specificities of neutralizing antibody elicited by antibody-based vaccines. (ox.ac.uk)

It is possible to achieve glycopeptide fragmentation under standard collision induced dissociation (CID) conditions, in which the glycosidic bonds between carbohydrate bonds are favourably cleaved. (news-medical.net)

An instance is the artificial glycopeptide CSF114(Glc), which was designed and examined as MS biomarker and whose scientific utility was restricted by its diminished capability to detect autoantibodies in MS sufferers. (korilog.com)
CSF114(Glc) is an artificial glucopeptide detecting in vitro MS-specific auto-antibodies, and it's actively utilized in diagnostics and analysis to observe and quantify MS-associated Ig ranges. (korilog.com)
Previously, antibodies specific to a gluco-asparagine (N-Glc) glycopeptide, CSF114(N-Glc), were identified in sera of an MS patient subpopulation. (unifi.it)

Yang's group developed a fine-tuned MS/MS acquisition method and pGlyco 2.0 search engine to perform confident intact glycopeptide characterization ( 13 ). (frontiersin.org)

Then, another breakthrough is that the addition of tetanus toxoid to glycopeptide conjugate resulted in a glycopeptide-TT vaccine induced dual (double) antibody-dependent protective immunity without the need for adjuvant, which is feasible for human use. (lsuhsc.edu)
Messenger RNAs are the current most promising vaccine strategy for inducing high-titered and long-lasting antibody responses. (grantome.com)

We specialize in making microarray-compatible glycans and glycopeptides. (zbiotech.com)
If you are working on released glycans ( N -linked or O -linked) or glycopeptides and are facing analytical challenges, our representative will be happy to discuss this in person during the conference. (premierbiosoft.com)

Tissues from cancer patients, for instance, have their own distinct pattern of Alpha-1-Antichymotrypsin expression as measured by anti-Alpha-1-Antichymotrypsin antibody immunohistochemical staining. (enquirebio.com)

10 kD, usually a protein or glycopeptide) that can cause production of specific IgE antibodies and typical allergic responses. (cdc.gov)

Vancomycin, a commonly used antibiotic for hospitalacquired infections, can cause severe, refractory thrombocytopenia due to antibody-mediated platelet destruction. (imedpub.com)
The findings emphasize the role of bacterial virulence factors in the pathogenesis of GAS infections and their potential impact on antibody-based therapies. (newsstatix.com)

Here we demonstrate that by using glycopeptides with high affinity for the major histocompatibility complex and glycosylated in a position corresponding to a critical T cell receptor (TcR) contact, it is possible to induce anti-TACA CTL in vivo. (silverchair.com)

The diagnosis of dystroglycanopathies is currently based on the observation of clinical manifestations, muscle biopsies and enzymatic measures, and the available monoclonal antibodies are not specific for the dystrophic hypoglycosylated muscle condition. (cnr.it)

Interestingly we revealed that a hyperglucosylated protein domain, based on the cell-surface adhesin HMW1A, is preferentially recognized by antibodies from sera of an MS patient subpopulation. (unifi.it)

It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear. (lookformedical.com)
Furthermore, the researchers discovered that bacterial virulence factors can also degrade exogenously administered IgG, impacting the efficacy of antibody-based therapies. (newsstatix.com)
It highlights the need for preclinical evaluations of therapeutic antibodies before their administration, as bacterial virulence factors can degrade and modify the structure and function of exogenous IgG. (newsstatix.com)

Typical glycopeptide CID spectrum achieved by using the Glycopeptide Instant Expertise™ method. (news-medical.net)

The platelet destruction is caused by a platelet-specific drugdependent antibody formation. (imedpub.com)
More importantly, 28 unique glycopeptides containing 5 core fucosylated bi- and tri-antennary structures, were uniquely discovered in HCC, suggesting that the site-specific unique glycoforms might be used to discriminate HCC from LC and HBV patients. (ntnu.edu.tw)

Glycopeptide antibiotic complex from Actinoplanes teichomyceticus active against gram-positive bacteria . (lookformedical.com)

AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. (enquirebio.com)

GlycoQuest (search algorithm in ProteinScape) is Bruker's proven solution and delivers dependable results with regards to glycopeptide identification. (news-medical.net)
Compound classification and ensuing glycopeptide identification is executed with ProteinScape 4.0 and the embedded search algorithm GlycoQuest. (news-medical.net)

Collision energy stepping implemented in the Glycopeptide Instant Expertise™ method. (news-medical.net)
After optimization, this one pot method enhanced the number (2-fold) and abundance (2-fold) of glycopeptides. (ntnu.edu.tw)

Variations in Alpha-1-Antichymotrypsin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. (enquirebio.com)

Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. (enquirebio.com)

Physiological role of CD52 on lymphocytes is unclear, although CAMPATH-1 antibody is capable of complement activation and it has been used in lympho proliferative disorders. (beckman.com)

As well as the instrumental arrangement, a suitable software solution is essential to enable glycopeptide identification and cut laborious processing and de novo analysis time. (news-medical.net)

Additionally, sera from both infected rhesus monkey and infected brown rat were capable of killing schistosomula in a manner inhibited by glycopeptides containing core Xyl/Fuc. (cdc.gov)

Regarding monoclonal antibodies, for the first time variable heavy (VH) and variable light (VL) immunoglobulin domains were selected by Phage Display, identified by NGS and described by in silico analysis. (cnr.it)

More recently, new high-throughput platforms have been introduced which are referred to as glycopeptide arrays (Table 1). (srcinhibitors.com)
High-multiplicity HIV-1 infection and neutralizing antibody evasion mediated by the macrophage-T cell virological synapse. (ox.ac.uk)

Two common sialylayted bi-antennary glycopeptides were identified in 10 of 11 patients for each cohort. (ntnu.edu.tw)

A vancomycin-dependent platelet antibody test was obtained after the patient received his sixth unit of platelets. (imedpub.com)

Anatomy8

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