Type IV collaPrimary antibodiesAntineutrophilAntigen-antibodyComplementTissueAntiglomerular basement membrane diseaseAlveolar basement membranesGlomerular basement membranesANCAELISAProteinsAntinuclear antibodyCytoplasmic antibodiesCollagenSpecific antibodiesImmunofluorescenceKidneysProteoglycanLamininKidneyActinHyalineEpitopesImmune complexesGoodpasture'sNephropathyEpitheliumPVDFDepositsPolyclonalPulmonaryExtracellularEpithelialAutoantibodiesReagentsSystemicNeutralizingCloneInflammatoryDilutionCapillariesProteinMolecular weightPlasma
Type IV colla12
- Anti-GBM antibodies are directed against an epitope located at the NC1 domain at the C-terminal of the alpha-3 chain of type IV collagen. (medscape.com)
- Antibodies reacting with the alpha-3 chain of type IV collagen can be detected in the serum and can be eluted from kidneys of patients with anti-GBM nephritis. (medscape.com)
- Basement membranes were stained with anti-type IV collagen. (nih.gov)
- The principal component of basement membrane is type IV collagen, which acts as a support structure and is composed of building blocks that are linked end-to-end. (medscape.com)
- Objective -To evaluate expression of the α6 chain of type IV collagen in the glomerular basement membranes (GBM) of healthy dogs. (avma.org)
- Thermal stability of the helical structure of type IV collagen within basement membranes in situ: determination with a conformation-dependent monoclonal antibody. (rupress.org)
- To examine the thermal stability of the helical structure of type IV collagen within basement membranes in situ, we have employed indirect immunofluorescence histochemistry performed at progressively higher temperatures using a conformation-dependent antibody, IV-IA8. (rupress.org)
- We conclude that the stability of the helical structure of type IV collagen within a basement membrane is considerably greater than it is in solution, and that conformation-dependent monoclonal antibodies can be useful probes for investigations of molecular structure in situ. (rupress.org)
- Laminin and type IV collagen are the two principal components of the basement membrane. (biogenex.com)
- Because type IV collagen and laminin are proteins found exclusively in the basement membrane, the antibodies to these two proteins selectively and specifically recognize basement membrane components. (biogenex.com)
- Type IV collagen is the main component of the basement membrane that gives strength to the blood-gas barrier (BGB). (biomedcentral.com)
- Our study showed that type IV collagen and, therefore the basement membrane, play fundamental roles in coordinating alveolar morphogenesis. (biomedcentral.com)
Primary antibodies1
- Conjugated primary antibodies with 490 nm, 550 nm, 594 nm, and 650 nm fluorophores! (antibodiesinc.com)
Antineutrophil2
- basement-membrane antibodies, and antineutrophil cyto- plasmic antibodies were negative. (cdc.gov)
- Antinuclear antibody and antineutrophil cytoplasmic antibody assays were negative. (who.int)
Antigen-antibody5
- Antigen-antibody complexes can develop by the production of immune complexes in situ or by deposition of circulating complexes. (medscape.com)
- This is a classic type II reaction in the Gell and Coombs classification of antigen-antibody reactions. (medscape.com)
- Changes in mucosal bacteria, and a switch from internal to surface antigen/antibody reactivity of a predominantly IgG1 type, leads to greater opsonisation of the respiratory burst in PMN, providing a mechanism for maintaining the inflammatory state in UC. (bmj.com)
- It has long been recognized that the thickening of the glomerular basement membrane (GBM) is a reaction to the injury of presumed antigen-antibody complexes depositing on the subepithelial surface of the GBM. (renalandurologynews.com)
- This antigen/antibody complex stimulates the formation of new basement membrane tissue that grows up and around the antigen-antibody complex deposits and creates thickening of the GBM when viewed down the microscope. (renalandurologynews.com)
Complement7
- When bound to the specific antigens in the kidneys and lungs, the antibodies initiate an inflammatory destruction of tissues by complement activation and recruitment of proinflammatory cells, leading to rapidly proliferative glomerulonephritis, often accompanied by pulmonary hemorrhage. (medscape.com)
- Once the anti-GBM antibodies bind to the specific GBM antigen, complement is activated. (medscape.com)
- Laboratory results were the increase in enterovirus antibody titration by complement following: leukocytes 9,600 cells ×106/L (90.6% neutro- fi xation between days 3 and 11. (cdc.gov)
- The autoantibodies bind to their reactive epitopes in the basement membranes and activate the complement cascade, resulting in tissue injury. (medscape.com)
- The anticollagen antibodies cross-react with GBM, fixing complement and triggering a cell-mediated inflammatory response in the kidneys and usually the lungs. (msdmanuals.com)
- Immunofluorescence showed diffuse deposition of glomerular basement membrane immunoglobulin (Ig)M and complement component C3, with mesangial IgG, but negative for C4, fibrin and IgA. (who.int)
- Diagnosis is made on the basis of the presence of a subepidermal vesicle on routine histologic examination and of linear deposition of the third component of complement along the basement membrane zone of perilesional skin. (medscape.com)
Tissue8
- Using a monoclonal anti-tubular basement membrane antibody (alpha TBM-Ab) affinity column, we isolated from collagenase-solubilized human renal tissue (HSRTA) a predominantly 48,000-mol-wt moiety (H3M-1) which is selectively recognized by antisera from two patients with alpha TBM-Ab-associated interstitial nephritis (alpha TBM disease). (jci.org)
- Although alveolar hemorrhage was the dominant pathologic finding in four of our five patients, all five patients had evidence of injury at the level of the alveolar wall, manifested by hyaline membranes and widening of alveolar walls by edematous connective tissue. (elsevierpure.com)
- We previously observed by competition enzyme-linked immunosorbent assay that, in neutral solution, the helical epitope to which this antibody binds undergoes thermal denaturation over the range of 37-40 degrees C. In the present study, we have reacted unfixed cryostat tissue sections with this antibody at successively higher temperatures. (rupress.org)
- human kidney tissue were subjected to SDS PAGE followed by western blot with 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. (ptglab.com)
- Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). (ptglab.com)
- Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). (ptglab.com)
- The tissue section was stained with ARG63621 anti-alpha smooth muscle Actin antibody at 3.5 µg/ml dilution followed by HRP-staining. (arigobio.cn)
- Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG21968 anti-Collagen IV antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin. (arigobio.cn)
Antiglomerular basement membrane disease1
- Neither pooled normal human sera nor sera from patients with a variety of renal lesions not associated with alpha TBM-Ab (including interstitial nephritis and antiglomerular basement membrane disease) were reactive with H3M-1. (jci.org)
Alveolar basement membranes4
- Anti-glomerular basement membrane (anti-GBM) disease is a classic autoimmune disorder characterized by the presence of circulating pathogenic autoantibodies directed against proteins in the glomerular and alveolar basement membranes. (medscape.com)
- it is found only in a few specialized basement membranes, including the glomerular and alveolar basement membranes. (medscape.com)
- This binding of antibodies can be visualized as the linear deposition of immunoglobulin along the glomerular basement membrane and, less commonly, the alveolar basement membranes, by direct immunofluorescent techniques. (medscape.com)
- Anti-GBM nephritis is a classic autoimmune disease characterized by the presence of circulating pathogenic autoantibodies directed against proteins in the glomerular and alveolar basement membranes. (medscape.com)
Glomerular basement membranes2
- The syndrome was subsequently shown to be caused by an antibody response against antigens present in the alveolar and glomerular basement membranes. (medscape.com)
- After 2 plamaphareses a renal biopsy showed: Light: endothelial swelling, thickening of glomerular basement membranes,with double contours & intraluminal thrombi. (asn-online.org)
ANCA3
- Coexistence of ANCAs (mostly myeloperoxidase [MPO-ANCAs]) with anti-GBM antibodies is thought to occur when the renal involvement in ANCA vasculitis leads to the exposure of antigens from the basement membrane and the formation of anti-GBM antibodies. (medscape.com)
- Double-antibody disease occurs with the presence of anti-GBM and ANCA antibodies. (msdmanuals.com)
- Anti-neutrophil cytoplasmic antibody (ANCA), anti-glomerular basement membrane (GBM) antibody, and antinuclear antibody findings were negative. (bvsalud.org)
ELISA4
- Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. (abcam.com)
- Find COL4A1 Antibodies validated for a specific application such as WB, ELISA, IHC, IHC (p). (antibodies-online.com)
- refer to Envoplakin Antibody, IgG by ELISA ( 3016533 ). (aruplab.com)
- refer to Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Expanded Antibody Panel by IIF With ELISA ( 3016534 ). (aruplab.com)
Proteins1
- Using antibodies to podocyte proteins important for podocyte function, we found that Lmx1b(-/-) podocytes express near-normal levels of nephrin, synaptopodin, ZO-1, alpha3 integrin, and GBM laminins. (nih.gov)
Antinuclear antibody1
- Laboratory examinations revealed mild leukocytosis and antinuclear antibody negativity. (bvsalud.org)
Cytoplasmic antibodies2
- Approximately 20-40% of patients positive for anti-GBM antibodies also have antineutrophilic cytoplasmic antibodies (ANCAs). (medscape.com)
- Although the anti-nuclear factor was positive (titre 1/160), the rheumatoid factor and extractable nuclear antibody screening tests were negative, while basement membrane antibodies and neutrophil cytoplasmic antibodies were not detected. (scielo.org.za)
Collagen7
- The fact that the anti-GBM antibodies are directed against the noncollagenous globular domain (NC1 domain) of the alpha-3 chain of type IV GBM collagen is well known. (medscape.com)
- This gene encodes the major type IV alpha collagen chain of basement membranes. (antibodies-online.com)
- Immunohistochemical staining was performed using antibodies against the major components of basement membranes such as laminin, heparan sulfate proteoglycan (HSPG), Collagen IV and fibronectin. (thieme-connect.com)
- In cholesteatoma epithelium, we found a fragmented basement membrane with small amounts of laminin and HSPG showing only occasional positively stained fragments while Collagen IV was almost completely absent. (thieme-connect.com)
- It may arise when respiratory exposures (eg, cigarette smoke, viral upper respiratory infection) or some other stimulus exposes alveolar capillary collagen, triggering formation of anticollagen antibodies. (msdmanuals.com)
- Over the years, tremendous gains have been made in our knowledge of the pathogenic mechanisms underlying anti-GBM nephritis, and the fact that the anti-GBM antibodies are directed against the noncollagenous globular domain (NC1 domain) of the alpha-3 chain of type IV GBM collagen is well known. (medscape.com)
- This antibody panel comprises the critical markers for angiogenesis research, including endothelial marker CD34 antibody, basement membrane marker Collagen IV antibody, and mural cell markers NG2 and alpha-SMA antibodies. (arigobio.cn)
Specific antibodies1
- The aim of this investigation was to compare the principal culturable bacterial populations on the rectal mucosa of UC patients, and to determine whether specific antibodies towards these bacteria can activate infiltrating PMN through opsonisation. (bmj.com)
Immunofluorescence3
- Immunofluorescence: hCMEC/D3 cells stained with ARG52756 anti-CD34 antibody [QBEND/10]. (arigobio.cn)
- Use to screen for IgG paraneoplastic pemphigus antibodies by indirect immunofluorescence using rat bladder and other rodent substrates. (aruplab.com)
- Direct immunofluorescence showed linear IgG staining along the basement membrane zone. (bvsalud.org)
Kidneys3
- [ 7 ] Immunofluorescent examination of affected kidneys demonstrated that these antibodies were localized in a continuous linear deposit along the GBM. (medscape.com)
- [ 6 ] confirmed that the antibodies taken from the diseased kidneys produced nephritis in experimental animals. (medscape.com)
- Deposits of antibodies build up in a part of the kidneys called the glomerular basement membrane. (mountsinai.org)
Proteoglycan2
- Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG59471 anti-NG2 / Chondroitin sulfate proteoglycan 4 antibody. (arigobio.cn)
- Western blot: A375 cell lysate stained with ARG59471 anti-NG2 / Chondroitin sulfate proteoglycan 4 antibody. (arigobio.cn)
Laminin3
- Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. (biogenex.com)
- In surgical pathology, laminin can be used as a marker to demonstrate morphologic change of basement membrane, which is helpful for interpreting the invasion of malignant tumors. (biogenex.com)
- In most patients, serum IgG antibodies react with laminin γ1. (bvsalud.org)
Kidney2
- In 1967, in a classic experiment, Lerner and colleagues transferred anti-GBM disease to monkeys by injecting them with kidney-bound antibodies from patients with anti-GBM nephritis. (medscape.com)
- Removing antibodies may reduce inflammation in the kidney tissues. (medlineplus.gov)
Actin2
- Western blot: 30 µg of MEF cell lysate stained with ARG63621 anti-alpha smooth muscle Actin antibody at 1:300 dilution. (arigobio.cn)
- Cells were stained with ARG63621 anti-alpha smooth muscle Actin antibody (green) at 10 µg/ml dilution for 1 hour. (arigobio.cn)
Hyaline1
- Renal biopsy revealed membranoproliferative glomerulonephritis, with lobulated glomerular tufts, endocapillary proliferation, thickened basement membrane, and glomerular hyaline thrombi suggestive of cryoglobulin deposits. (who.int)
Epitopes2
- We conclude, therefore, that tubular basement membrane target epitopes and their paratypic recognition are highly conserved among mammals. (jci.org)
- [ 5 , 6 ] Two conformational epitopes of anti-GBM antibodies have been defined at residues 17-31 and 127-141 of alpha3(IV)NC1, which were named as EA and EB, respectively. (medscape.com)
Immune complexes1
- Membranous nephropathy is an autoimmune disorder in which immune complexes deposit along the subepithelial region of the glomerular basement membrane. (medscape.com)
Goodpasture's2
- Anti-glomerular basement membrane antibody (anti-GBM Ab) is an antibody which is found in Goodpasture's syndrome but not found in microscopic polyangiitis. (wikipedia.org)
- We report the findings of lung biopsies from five patients with anti-basement membrane antibody-associated Goodpasture's syndrome (ABMA-GS). (elsevierpure.com)
Nephropathy6
- [ 4 ] Anti-PLA2R antibodies have been found in patients with viral infections (eg, hepatitis B, hepatitis C, HIV), but those patients may have had coincidental primary membranous nephropathy. (medscape.com)
- [ 6 ] Detection of anti-PLA2R antibodies in glomeruli but not in liver parenchyma is a common finding in phatients with membranous nephropathy associated with autoimmune liver disease, suggesting that these autoantibodies are not exclusive to idiopathic membranous nephropathy. (medscape.com)
- [ 8 , 9 ] Hoxha et al reported expression of THSD7A in a gallbladder carcinoma, in a patient who developed membranous nephropathy with anti-THSD7A antibodies, and subsequently found anti-THSD7A antibodies in six other patients with membranous nephropathy and malignant tumors, suggesting that THSD7A production by malignancies is a possible mechanism for membranous nephropathy. (medscape.com)
- Debiec et al reported that four of nine patients with childhood membranous nephropathy had high levels of circulating anti-bovine serum albumin antibodies and circulating cationic bovine serum albumin. (medscape.com)
- Expression of glomerular basement membrane (GBM) collagens is reduced in Lmx1b(-/-) mice, suggesting one basis for NPS nephropathy. (nih.gov)
- What might your study reveal about thickening of the glomerular basement membrane-the cause of membranous nephropathy? (renalandurologynews.com)
Epithelium1
- The basement membranes are complex structures that support layers of endothelium and epithelium. (medscape.com)
PVDF1
- PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse SPARC Monoclonal Antibody (Catalog # MAB942) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). (rndsystems.com)
Deposits1
- EM: corrugated basememnt membranes with total foot process fusion and no immune deposits. (asn-online.org)
Polyclonal1
- Find available monoclonal or polyclonal COL4A1 Antibodies. (antibodies-online.com)
Pulmonary4
- In Goodpasture syndrome , glomerulonephritis is accompanied by pulmonary hemorrhage due to anti-GBM antibodies acting on the alveolar basement membrane. (medscape.com)
- Goodpasture disease is a term used to describe glomerulonephritis, with or without pulmonary hemorrhage, and the presence of circulating anti-glomerular basement membrane (anti-GBM) antibodies. (medscape.com)
- Goodpasture Syndrome Goodpasture syndrome, a type of pulmonary-renal syndrome, is an autoimmune syndrome consisting of alveolar hemorrhage and glomerulonephritis caused by circulating anti-glomerular basement membrane. (msdmanuals.com)
- Goodpasture syndrome can be defined by the presence of a triad of glomerulonephritis, pulmonary hemorrhage, and anti-GBM antibodies. (medscape.com)
Extracellular1
- The metalloproteinase-9 (MMP-9) or gelatinase B belongs to the family of matrix metalloproteinases, enzymes which degrade extracellular matrix (ECM) and basement membrane components and are implicated in the carcinogenesis process, contributing to tumor progression, invasion and metastasis. (bvsalud.org)
Epithelial2
- An analysis of the basement membrane, which can modulate epithelial proliferation, reveals a dysfunction of this layer between cholesteatoma and stroma. (thieme-connect.com)
- This staining pattern resembles the initial rear-rangement of the epithelial basement membrane in human skin wounds and provides evidence for a lost contact inhibition on the provisional fibronectin-layer as a reason for the hyperproliferative nature of cholesteatoma lesions. (thieme-connect.com)
Autoantibodies1
- Anti-GBM disease is an autoimmune disorder characterized by autoantibodies directed against the glomerular/alveolar basement membrane. (medscape.com)
Reagents1
- These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans. (antibodiesinc.com)
Systemic1
Neutralizing1
- will it lead to possibly six different neutralizing antibodies? (12160.info)
Clone1
- Find COL4A1 Antibodies with a specific Clone. (antibodies-online.com)
Inflammatory1
- This combination of effector cells requires a crosslinking antigen to evoke an antibody driven PMN inflammatory response via their Fc receptors. (bmj.com)
Dilution1
- Western blot: 30 µg of mouse lung lysate stained with ARG52756 anti-CD34 antibody [QBEND/10] at 1:500 dilution. (arigobio.cn)
Capillaries1
- Under these conditions, the in situ denaturation temperature of this epitope in most basement membranes is 50-55 degrees C. In capillaries and some other small blood vessels the fluorescent signal is still clearly detectable at 60 degrees C, the highest temperature at which we can confidently use this technique. (rupress.org)
Protein1
- It is present in cow's milk and beef protein and can escape the intestinal barrier and cause antibody formation. (medscape.com)
Molecular weight2
- Albumin makes the blood more viscous and it's half the molecular weight of antibodies. (12160.info)
- Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band. (antibodiesinc.com)
Plasma2
- The fluid part of the blood that contains antibodies is removed and replaced with intravenous fluids or donated plasma (that does not contain antibodies). (medlineplus.gov)
- The mucosa in ulcerative colitis (UC) is replete with antibody producing plasma B cells and polymorphonuclear leucocytes (PMN). (bmj.com)