• The following product was used in this experiment: CD5 Monoclonal Antibody (53-7.3), NovaFluor™ Red 725, eBioscience™ from Thermo Fisher Scientific, catalog # M026T03R05, RRID AB_2926168. (thermofisher.com)
  • C57BL/6 mouse splenocytes were unstained (left) or stained with 0.2 µg of CD5 Monoclonal Antibody, NovaFluor Red 725 (Product # M026T03R05) (right). (thermofisher.com)
  • All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). (thermofisher.com)
  • Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. (thermofisher.com)
  • The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes). (exbio.cz)
  • Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. (exbio.cz)
  • Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. (bdbiosciences.com)
  • CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. (thermofisher.com)
  • Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. (exbio.cz)
  • In B-PLL patients there is strong expression of surface immunoglobulin - a membrane-bound form of an antibody, b-lymphocyte surface antigens CD19, CD20, CD22, CD79a and FMC7, and weak expression of CD5 and CD23. (wikipedia.org)
  • The lymphocyte receptor CD5 influences cell activation by modifying the strength of the intracellular response initiated by antigen engagement. (ox.ac.uk)
  • CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72. (biolegend.com)
  • CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction. (thermofisher.com)
  • CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). (exbio.cz)
  • CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. (exbio.cz)
  • The team adapted mRNA delivery technology seen in current COVID-19 vaccines and applied it to basic Chimeric Antigen Receptor design. (medika.life)
  • Three-dimensional solution structure and conformational plasticity of the N-terminal scavenger receptor cysteine-rich domain of human CD5. (ox.ac.uk)
  • Regulation through CD5 involves the interaction of one or more of its three scavenger receptor cysteine-rich domains present in the extracellular region. (ox.ac.uk)
  • The results suggest that the novel protein is a macrophage-specific membrane receptor with a role in host defense, as it shows postnatal expression in macrophages, which are considered responsible for the binding of bacterial antigens and phagocytosis. (embl.de)
  • The extracellular domain of CD6 is significantly related to the extracellular domain of the human and mouse T cell antigen CD5, the cysteine-rich domain of the bovine and mouse type I macrophage scavenger receptor, the extracellular domain of the sea urchin spermatozoa protein that crosslinks the egg peptide speract, the mammalian complement factor 1, and the human lung tumor antigen L3. (embl.de)
  • 3. Stimulation of CD5 Enhances Signal Transduction by the T cell Antigen Receptor. (exalpha.com)
  • CD5 Antigen-like (CD5L), also known as Sp alpha, is a secreted protein that is a member of the SCSR (scavenger receptor cysteine rich) superfamily. (rndsystems.com)
  • Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. (bvsalud.org)
  • However, chimeric antigen receptor T (CAR-T) cell immunotherapy and immune checkpoint blockade (ICB), which have produced unprecedented clinical benefits in a variety of different cancers, produce promising results in only some highly selected patients with PDAC. (biomedcentral.com)
  • According to the kinetic proofreading model, antigen discrimination properties could be explained by the gradual amplification of small differences in binding affinities as the signal is transduced downstream of the T cell receptor. (nature.com)
  • The CD5 antibody, clone L17F12, is derived from the hybridization of NS-1/Ag4 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human T-acute lymphoblastic leukemia (ALL) cells. (bdbiosciences.com)
  • Staining of porcine peripheral blood lymphocytes with FITC cojugated Mouse anti Porcine CD5 antibody, clone 1H6/8 ( MCA2307F ). (bio-rad-antibodies.com)
  • Figure A. FITC conjugated Mouse anti Porcine CD5 antibody, clone 1H6/8 ( MCA2307F ) and RPE conjugated Mouse IgG2a isotype control ( MCA929PE ). (bio-rad-antibodies.com)
  • Figure B. FITC conjugated Mouse anti Porcine CD5 antibody, clone 1H6/8 ( MCA2307F ) and RPE conjugated Mouse anti Porcine CD4α antibody, clone MIL17 ( MCA1749PE ). (bio-rad-antibodies.com)
  • Figure A. RPE conjugated Mouse anti Porcine CD4α antibody, clone MIL17 ( MCA1749PE ) and FITC conjugated Mouse IgG2b isotype control ( MCA691F ). (bio-rad-antibodies.com)
  • Figure A. FITC conjugated Mouse anti Porcine CD5 antibody, clone 1H6/8 ( MCA2307F ) and RPE conjugated mouse IgG1 isotype control ( MCA928PE ). (bio-rad-antibodies.com)
  • Figure B. FITC conjugated Mouse anti Porcine CD5 antibody, clone 1H6/8 ( MCA2307F ) and RPE conjugated Mouse anti Porcine CD8α antibody, clone MIL12 ( MCA1223PE ). (bio-rad-antibodies.com)
  • COS cells transfected with the CD6 clone express a 90-kD protein that reacts with all available anti-CD6 monoclonal antibodies. (embl.de)
  • Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells. (bio-rad-antibodies.com)
  • Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes. (bio-rad-antibodies.com)
  • CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. (biolegend.com)
  • The antibody MEM-98 reacts with an extracellular epitope of CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells. (exbio.cz)
  • For IHC analysis, anti-human CD5 monoclonal mouse antibody to identify T lymphocytes, CD20 monoclonal mouse antibody for the identification of mature B lymphocytes and HLA-DR antigen (alpha-chain) monoclonal mouse antibody for the identification of the major histocompatibility complex type II were previously validated for both species by Western-blotting technique. (ulpgc.es)
  • He underwent a repeat kidney biopsy which demonstrated MN with 20% interstitial infiltrate that was PAX5, CD23, CD20 and CD5 stain positive consistent with clonal B-cell lymphoproliferation with features of CLL. (asn-online.org)
  • Abbit secondary antibody and DAB chromogen. (icbinhibitor.com)
  • Other parameters such as serum dilutions, secondary antibody concentration, incubation temperature and time combinations were successfully standardized. (porkcheckoff.org)
  • Figure B. RPE conjugated Mouse anti Rat MHC-I ( MCA156PE ) and FITC conjugated Mouse anti Rat CD5 ( MCA52F ). (bio-rad-antibodies.com)
  • These T cells target either CD19 or BCMA, two antigens found on malignant B cells. (medika.life)
  • In contrast, most markers (CD3, CD5, CD13, CD14, CD19, CD45, HLA-DR, and TdT) showed strong correlation between PB and BM samples in AML group.Conclusions:The findings of this study suggests that targeted gating strategy for blast population as well as selection of a suitable panel of monoclonal antibodies may be essential for diagnosis of leukemia resulting in similar immunotyping pattern in PB and BM. (medscimonit.com)
  • Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. (exbio.cz)
  • The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. (thermofisher.com)
  • Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. (thermofisher.com)
  • Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells. (exbio.cz)
  • Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. (exbio.cz)
  • CD5 is intended for in vitro diagnostic use in the identification of cells expressing CD5 antigen, using a BD FACS™ brand flow cytometer. (bdbiosciences.com)
  • Phenotypic and functional characterization of human Leu-1 (CD5) B cells. (bdbiosciences.com)
  • Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. (bdbiosciences.com)
  • Human T-cell antigens defined by monoclonal antibodies: The 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. (bdbiosciences.com)
  • The lymphocytic clusters in SM contained nearly equal numbers of mature T and B cells, the latter with no coexpression of aberrant antigens, such as CD5 or CD23. (bmj.com)
  • Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone). (exbio.cz)
  • B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)
  • 1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
  • The purpose of this study was to test the hypothesis that anti-VEGF antibody, bevacizumab, promotes in vitro tenogenic differentiation and maturation of two distinct types of TSPCs, tendon proper-derived cells (TDCs), and paratenon-derived cells (PDCs) originating from rat Achilles tendon. (bvsalud.org)
  • Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85% peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells. (exalpha.com)
  • 1. Anti-DNA Antibody Production by CD5+ and CD5- B Cells of Patients with Systemic Lupus Erythematosus. (exalpha.com)
  • Cluster of differentiation 5 (CD5) is expressed in high levels on the surface of T cells, while controversy surrounds the expression levels and role of CD5 in B cells. (abeomics.com)
  • As a part of a diagnostic panel, its utility lies predominantly as a marker for T cells, with over 70% of T cell neoplasms expressing CD5. (abeomics.com)
  • 6. In Vivo and In Vitro Expression of Myeloid Antigens on B-lineage Acute Lymphoblastic Leukemia Cells. (nordicmubio.com)
  • This phenomenon could be offset by a dual-CAR system incorporating both an activating CAR against the cognate tumor antigen and an NK self-recognizing inhibitory CAR that transferred a 'don't kill me' signal to NK cells upon engagement with their TROG + siblings. (nature.com)
  • B-1 cells are found in peritoneal and pleural cavities where they provide first-line defence through antibodies able to bind polysaccharide antigens and repeated motifs that are typically found in microbial cell walls and macromolecules [ 2 , 3 ]. (biomedcentral.com)
  • Specific, monoreactive antibodies from the adaptive B-cell system (the large, B-2 subset) are produced later, after the activation and recruitment of T-cells. (biomedcentral.com)
  • Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)
  • The depletion cocktail in this kit does not contain antibodies against CD43, which can be expressed on malignant cells in chronic lymphocytic leukemia (CLL) samples. (stemcell.com)
  • This cell isolation kit targets non-B cells for removal based on specific surface antigens. (stemcell.com)
  • Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. (stemcell.com)
  • T cells recognize a few high-affinity antigens among a vast array of lower affinity antigens. (nature.com)
  • Here, we used time-resolved high-throughput proteomic analyses to identify and quantify the phosphorylation events and protein-protein interactions encoding T cell ligand discrimination in antigen-experienced T cells. (nature.com)
  • Human peripheral blood lymphocytes were stained with purified CD5 (UCHT2) (solid line) or purified mouse IgG1, κ (dashed line), followed by anti-mouse IgG PE. (biolegend.com)
  • CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. (exbio.cz)
  • Separation of human CD6 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD6 (MEM-98) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
  • A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
  • 4. Surface Immunoglobulin Ligands and Cytokines Differentially Affect Proliferation and Antibody Production by Human CD5+ and CD5B Lymphocytes. (exalpha.com)
  • 4. Surface Immunoglobulin Ligands and Cytokines Differentially Affect Proliferation and Antibody Production by Human CD5+ and CD5- B Lymphocytes. (nordicmubio.com)
  • This system prevented trogocytic antigen-mediated fratricide, while sparing activating CAR signaling against the tumor antigen, and resulted in enhanced CAR-NK cell activity. (nature.com)
  • The patient was seropositive for HTLV-1 by Western blot and positive for hepatitis B surface antigen. (cdc.gov)
  • Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. (bdbiosciences.com)
  • Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD6 (MEM-98) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
  • Therefore, antigen binding and stimulation could contribute to clonal selection and expansion as well as disease promotion. (nih.gov)
  • The CD5 antibody is composed of mouse IgG2a heavy chains and kappa light chains. (bdbiosciences.com)
  • Immunophenotyping helps distinguish B-PLL from similar diseases, one of its key identifiers is the absence in expression of the surface antigens CD10, CD11c, CD25, CD103 and cyclin D1 - an important regulator of cell-cycle progression. (wikipedia.org)
  • CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. (abeomics.com)
  • Fig. 4: A lower level of CAR-mediated TROG-antigen expression was associated with improved clinical response to CAR-NK cell-based immunotherapy. (nature.com)
  • To achieve project objectives, VP7 sequences of G5 RVA and G6 RVC (sequenced from the feedback material) were cloned into pcDNA3.1(+) mammalian expression vector with a CD5 secretory signal for efficient secretion into the culture media. (porkcheckoff.org)
  • The VP7 (G genotypes) is the most immunogenic, highly glycosylated, independently elicit neutralizing antibodies, and induce protective immunity. (porkcheckoff.org)
  • Since apoptosis can make autoantigens accessible for recognition by antibodies, and also create neo-epitopes by chemical modifications occurring naturally during this process, we sought to determine if CLL mAbs recognize autoantigens associated with apoptosis. (nih.gov)
  • Several functions have been proposed for natural antibodies, including a first-line role in the defense against infections, a scavenger-like activity to apoptosis by-products and a turn-off, regulative role in the maintenance of immune homeostasis [ 7 , 14 ]. (biomedcentral.com)
  • Western blot: 10 µg of Jurkat cell lysate stained with ARG66751 anti-CD5 antibody [SQab20184] at 1:1000 dilution. (arigobio.cn)
  • Chronic lymphocytic leukemia (CLL) represents the outgrowth of a CD5(+) B cell. (nih.gov)
  • Development and characterization of new monoclonal antibodies against porcine Interleukin-17A and Interferon-gamma. (usda.gov)
  • The B-cell-specific molecule CD72 is a natural ligand for CD5. (bvsalud.org)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. (exbio.cz)
  • Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc. (exbio.cz)
  • Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. (bdbiosciences.com)
  • Immunochemical analysis of the T-cell-specific antigens. (bdbiosciences.com)
  • Warnke RA, Levy R. Detection of T and B cell antigens with hybridoma monoclonal antibodies: a biotinavidin-horseradish peroxidase method. (bdbiosciences.com)
  • 5. Evidence for Differential Responsiveness of Human CD5+ and CD5- B cell Subsets to T cell Independent Mitogens. (exalpha.com)
  • CD5 also has value as a prognostic indicator, being associated with poor prognosis in acute T cell lymphoblastic leukemia. (abeomics.com)
  • Fig. 2: Impact of antigen-induced self-engagement on CAR-NK effector cell phenotype and function. (nature.com)
  • Fig. 3: Impact of TROG-antigen acquisition on CAR-NK cell phenotype and function in vivo. (nature.com)
  • CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape. (nature.com)
  • Some RVA and most, if not all, RVC strains are extremely difficult to adapt to cell culture, and serological assays are unavailable to measurement of the immune response and antibody levels in response to natural planned exposure (NPE) protocols. (porkcheckoff.org)
  • Expi293 cell suspension cultures were transfected with pcDNA3.1+ constructs expressing the rotavirus antigens (G5 RVA and G6 RVC). (porkcheckoff.org)
  • The antibody was purified by affinity chromatography. (biolegend.com)
  • The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. (bdbiosciences.com)
  • Recombinant full length protein of Human CD5 (extracellular). (arigobio.cn)
  • Here, we present the 3D solution structure of a non-glycosylated double mutant of the N-terminal domain of human CD5 expressed in Escherichia coli (eCD5d1m), which has enhanced solubility compared to the non-glycosylated wild-type (eCD5d1). (ox.ac.uk)
  • Human serum usually contains natural IgG, IgM and IgA antibodies, generated independently of any exposure to foreign antigens or vaccines or elicited in the course of infectious or autoimmune diseases. (biomedcentral.com)
  • In other words, if the antigen-antibody reaction is compared to a "key-and-lock" model, natural antibodies found in human secretions act as "passe-partout" keys to offer a background protection against most pathogens, food antigens and microbes, before the antigen-specific response can develop [ 4 ]. (biomedcentral.com)
  • Anti-his cobalt resin (TALON, Takara Bio) was used for purification of the the recombinant antigens from culture media under native conditions. (porkcheckoff.org)
  • CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. (exbio.cz)
  • In serum and colostrum, the primary antibody is IgG, which is transferred from the serum to colostrum during gestation and absorbed into the piglet's serum upon ingestion of colostrum. (porkcheckoff.org)
  • The primary antibody in the intestine is IgA, which is transferred to piglets during ingestion of milk and protects against enteric infections. (porkcheckoff.org)