• This antibody binds to rat leukosialin, and can be used to label thymocytes and T lymphocytes in the lymph node cell population in flow cytometry analysis. (absoluteantibody.com)
  • The S7 monoclonal antibody specifically binds to the 115 kDa glycosylated form of CD43 (Ly-48, Leukosialin). (bdbiosciences.com)
  • Finally, the mAb 1B11 binds mouse CD43 only when modified with core 2 O-glycans (31). (geogise.com)
  • Right panel: antigen binds to the BCR of antigen-specific B cells and is internalized. (hindawi.com)
  • This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • DX5 is a monoclonal antibody which binds to CD49b. (wikidoc.org)
  • Through the development of an anti-ICAM1 antibody against the newly found epitope, he succeeded in inducing antigen-specific T cell tolerance via the development of tolerogenic semi-mature dendritic cells (J Exp Med. (wikipedia.org)
  • His team treated the monkeys with the anti-ICAM1 epitope antibody before the xenotransplantation process, expecting the induction of immune-tolerance to the pancreatic antigens of other species in the non-human primates. (wikipedia.org)
  • The antibody MEM-31 recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. (exbio.cz)
  • The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. (absoluteantibody.com)
  • However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility. (absoluteantibody.com)
  • BACKGROUND: JL1, a CD43 epitope and mucin family cell surface glycoprotein, is expressed on leukemic cells. (koreamed.org)
  • Likewise, antigen/antibody complexes are bound by the FcR of APCs and processed in a unique fashion dependent on the epitope specificity of the bound antibody. (hindawi.com)
  • van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. (exbio.cz)
  • The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody. (absoluteantibody.com)
  • To clarify the phenotypes of human hematopoietic stem cells (HSCs), we initially prepared novel antibodies against CD34 and labeled one of them (4A1) with allophycocyanin (APC). (elsevierpure.com)
  • When CD18 or CD11a antibody was added to a coculture system of CD34 + CD33 - cells with stromal layers, the number of generated CFCs decreased significantly compared with the no antibody control. (elsevierpure.com)
  • Similar to CD28 antibodies, this mAb, R2/60, was found to synergize with T cell receptor engagement in inducing proliferation. (nebraska.edu)
  • Together, these data demonstrate that CD43 can serve as a receptor on T cells that can provide CD28-independent costimulation. (nebraska.edu)
  • At low amounts, membrane-bound antigens allow survival of B cells bearing a single high affinity self-reactive B cell receptor (BCR). (silverchair.com)
  • In transgenic mice expressing hen egg lysozyme (HEL) as a neo self-antigen, B cells transgenic for a high affinity B cell receptor (BCR) specific for HEL undergo different fates ( 2 , 3 ). (silverchair.com)
  • Description: This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64, a high affinity Fc binding receptor also known as Fc-gammaR1 or Fcgr1. (thermofisher.com)
  • It has been proposed that long-lasting B cell ediated immunity is sustained by recurrent antigen exposure and within the absence of cognate antigen, inflammatory stimuli related with adaptive immune responses like cytokines, Toll-like receptor (TLR) agonists or T cell aid drive the activation of Bmem in an non-specific manner in vivo [10,11]. (cathepsin-s.com)
  • The development of monoclonal antibodies that are able to recognize specific glycan motifs on individual proteins has not been rigorously pursued. (geogise.com)
  • Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.co.jp)
  • The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues. (biolegend.com)
  • Leukocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) was expressed on some populations of hematopoietic cells and contributed to the proliferation by interacting with stromal cells. (elsevierpure.com)
  • Each clinicopathologic subtype can be further classified with respect to morphology, immunophenotype, and antigen markers. (medscape.com)
  • HI149 is an anti-human monoclonal antibody that forms an immune complex with the CD1a antigen. (gentaur.bg)
  • Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
  • ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
  • Autoantibodies can bind to basic structural molecules and interfere with the synthesis of structural elements and facilitate the uptake of antigen. (hindawi.com)
  • After processing, antigen is presented on MHC molecules. (hindawi.com)
  • The venom promoted the differentiation of Bmem and subtypes of ASC that have been characterized by the expression of B220 and CD43 molecules (B220 highCD43high, B220 highCD43low, B220 lowCD43high or B220 negCD43high), indicating a hierarchical course of action of differentiation [13]. (cathepsin-s.com)
  • A. The linear model describes the activation of naïve T-cells by the presentation of antigen-peptide on major histocompatibility complex (MHC) molecules by the antigen presenting cells (APCs) during the contraction phase of a T-cell response. (flowmetric.com)
  • Interestingly, both MO-MDSCs and PMN-MDSCs suppressed antigen-specific T-cell responses, albeit using distinct effector molecules and signaling pathways. (ashpublications.org)
  • After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. (absoluteantibody.com)
  • A diagnosis of common variable immunodeficiency (CVID) is based on defective functional antibody formation, usually accompanied by decreased (not absent) serum immunoglobulin (Ig)G and IgA levels, generally (not invariably) decreased serum IgM, and exclusion of other known causes of antibody deficiency. (medscape.com)
  • The common immunologic defect in patients with common variable immunodeficiency is defective antibody formation. (medscape.com)
  • The depletion cocktail in this kit does not contain antibodies against CD43, which can be expressed on malignant cells in chronic lymphocytic leukemia (CLL) samples. (stemcell.com)
  • This cell isolation kit targets non-B cells for removal based on specific surface antigens. (stemcell.com)
  • Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. (stemcell.com)
  • cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
  • In a search for other costimulatory receptors on T ceils, we have characterized a monoclonal antibody (mAb) that can costimulate T cells in the absence of accessory cells. (nebraska.edu)
  • To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
  • CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. (bdbiosciences.com)
  • [ 29 , 30 ] Although the molecular mechanisms are not fully understood, they are believed to involve stimulation of antigen receptors by autoantigens, and co-stimulatory molecule CD40 by H pylori -specific T cells. (medscape.com)
  • Bulk RNA-sequencing of CD43- sorted CD19-Cre+/-Prkaa1-/- (KO) and CD19-Cre+/-Prkaa1+/+ (WT) splenic naïve B cells (n = 3 each genotype), stimulated using CD40L + IL4 in vitro to terminal differentiation over 5 days (n = 3 each genotype at 5 timepoints plus naive, 36 samples total). (nih.gov)
  • The ligand for the HECA-452 mAb Dinaciclib (SCH 727965) is cutaneous lymphocyte antigen (CLA), which is often used in human samples to identify T cells that can bind to E-selectin and have skin homing potential (28, 29). (geogise.com)
  • Although B1 cells have been recognized for their protective functions against viruses, bacteria and parasites by producing natural IgM antibodies [ 3 ], increasing evidence indicates that B1 cells, especially the highly polyspecific B1a cells, play a role in autoimmune pathogenesis [ 4 ]. (oncotarget.com)
  • Through these mechanisms B cells are involved both in autoimmune diseases that are traditionally viewed as antibody mediated and also in autoimmune diseases that are commonly classified as T cell mediated. (hindawi.com)
  • Likewise B cells function as crucial antigen presenting cells in autoimmune diseases that are traditionally viewed as T cell mediated. (hindawi.com)
  • B cells have antibody-dependent and antibody-independent pathogenic functions. (hindawi.com)
  • Independent of antibody secretion B cells secrete proinflammatory cytokines, support the formation of ectopic GCs, and serve as antigen presenting cells. (hindawi.com)
  • Both secreted autoantibodies and BCR on B cells can modulate the processing and presentation of antigen and thereby affect the nature of presented T-cell determinants. (hindawi.com)
  • Binding of the Fc portion of the antibodies to FcR leads to activation of effector cells and further release of proinflammatory cytokines and proteolytic enzymes, mediators of antibody-dependent cell-mediated cytotoxicity (ADCC). (hindawi.com)
  • c) Effect of antibodies and antigen-specific B cells on antigen uptake. (hindawi.com)
  • Left panel: antigen bound by antibody is taken up via FcR on APCs such as dendritic cells or macrophages. (hindawi.com)
  • B cells are highly efficient APCs in situations of low antigen concentrations. (hindawi.com)
  • Here, we demonstrate that developing self-reactive B cells are not always highly sensitive to the deletion mechanisms imposed by membrane-bound self-antigens. (silverchair.com)
  • low quantities of self-antigen induce the selection and accumulation of increased numbers of self-reactive B cells with decreased expression of antigen-specific BCRs. (silverchair.com)
  • By directly measuring antigen binding by intact B cells, we show that the low amounts of self-antigen select self-reactive B cells with a lower association constant. (silverchair.com)
  • A fraction of these B cells is activated and secretes autoantibodies that form circulating immune complexes with self-antigen. (silverchair.com)
  • These findings demonstrate that conventional B cells can undergo positive selection and that the fate of a self-reactive B cell depends on the quantity of self-antigen, the number of BCRs engaged, and on its overall antigen-binding avidity, rather than on the affinity of individual BCRs. (silverchair.com)
  • When HEL was expressed in a soluble form, HEL-specific B cells were not deleted, but became unable to proliferate or secrete HEL-specific antibodies ( 2 ). (silverchair.com)
  • Applications Tested: This X54-5/7.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. (thermofisher.com)
  • After antigen stimulation, these cells migrate into tissue areas that are inflamed where they exhibit a strong proliferative response and express and release cytokines that help to eliminate infection. (flowmetric.com)
  • These are referred to as CD8+ memory T-cells because they 'remember' this previous encounter with the specific antigen, and they are programmed to elicit a faster and stronger immune response to that same antigen. (flowmetric.com)
  • Flow cytometry analysis of CD19 was performed by staining Raji CD19 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82, yellow histogram) or 0.25 µg CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ (Product # 12-0199-42, blue histogram). (thermofisher.com)
  • Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. (exbio.cz)
  • Describes the original use of this antibody, along with other anti-CD43 MAbs, in FC, WB and plate binding assay studies to determine the dimensions of the extracellular part of the leukosialin molecule, and to establish that the antigenic determinants recognized are largely based on linear protein sequences. (absoluteantibody.com)
  • This assessment can also be done by measuring antibody responses following active immunization with protein or polysaccharide antigens. (medscape.com)
  • In children who have completed immunizations with diphtheria, pertussis, and tetanus (DPT) or Hib-conjugated vaccines, the antibody response to protein antigens can be tested in adults and older children by measuring IgG antibodies to tetanus or diphtheria toxoid and H influenzae type b (Hib) polysaccharide antigen. (medscape.com)
  • Assessment of responses to polysaccharide antigens is important in patients older than 18-24 months because these responses may be deficient in some patients who can respond normally to protein antigens. (medscape.com)
  • Protein conjugated pneumococcal vaccine elicit antibody responses that are T-cell dependent. (medscape.com)
  • Therefore, antibody responses should be measured to polysaccharide antigens that are not present in the protein-conjugated pneumococcal vaccine. (medscape.com)
  • Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OX-58 (Ab00586-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. (absoluteantibody.com)
  • Based on these results, it was postulated that B cell deletion required BCR cross-linking and could only be induced by membrane bound self-antigens. (silverchair.com)
  • This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes. (absoluteantibody.com)
  • This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. (absoluteantibody.com)
  • Expression doning of the R2/60 antigen identified the ligand as murine CD43. (nebraska.edu)
  • The non-proliferating ASC secrete higher affinity antigenspecific PDE5 Inhibitor Purity & Documentation antibodies (Abs) for protracted periods of time [1,4], are capable of homing to bone marrow (BM) by means of CXCR4/ CXCL12-mediated chemokine signaling or inflamed tissue and differ from Bmem in many respects. (cathepsin-s.com)
  • CD8+ T-cell mediated immune responses are important in protection against various pathogens and specific tumor antigens associated with various cancers. (flowmetric.com)
  • He also found the novel antigen JL1 on thymocytes, which has been developed as a therapeutic target for leukemia. (wikipedia.org)
  • The Fc portion of antibodies in immune complexes can be bound by C1q of the classical complement pathway, which eventually leads to the release of C5a and C3a. (hindawi.com)
  • IgG subclass determination is of limited value in assessing patients with clinical immunodeficiency because functional antibody deficiency may be present despite normal IgG subclass levels. (medscape.com)
  • Assessment of the ability to produce functional antibodies can be obtained by measuring antibody responses to natural antigens or those antigens to which the population commonly is exposed. (medscape.com)
  • This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.co.jp)
  • Figure B. RPE conjugated Mouse anti Rat CD45Ra ( MCA340PE ) and FITC conjugated Mouse anti Rat CD43 ( MCA54F ). (bio-rad-antibodies.com)
  • Figure A. FITC conjugated Mouse anti Rat CD43 ( MCA54FT ) and RPE conjugated Mouse IgG1 isotype control ( MCA1209PE ). (bio-rad-antibodies.com)
  • Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody ( MCA54GA ) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody ( STAR132P ) for detection. (bio-rad-antibodies.com)
  • Early activation of Ampk by pharmacological means also has little impact on B cell function, although treatment with the biguanide Phenformin critically impairs germinal center formation and class switch recombination in vivo, but does not significantly impair antigen-specific antibody responses. (nih.gov)
  • An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)
  • Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion. (bdbiosciences.com)
  • Also it is actually not clear whether or not antigen reencounter benefits within the activation of antigenresponding Bmem or if intrinsic changes modulate their differentiation into ASC following appropriate stimulation [9]. (cathepsin-s.com)
  • Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. (thermofisher.com)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (thermofisher.com)
  • The following product was used in this experiment: CD19 Monoclonal Antibody (HIB19), PE, eBioscience™ from Thermo Fisher Scientific, catalog # 12-0199-42, RRID AB_1834376. (thermofisher.com)
  • Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. (thermofisher.com)
  • Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. (thermofisher.com)
  • Another important achievement of his research is the induction of antigen-specific T cell tolerance, which has been a distant dream of immunologists for pinpoint-targeted control of the immune system. (wikipedia.org)
  • MECA-79 is a mAb that reacts to 6-sulfo Lex on core 1 O-glycans and is used to identify HEVs (or HEV-like structures) and this antibody can sufficiently block naive T cell homing to secondary lymphoid organs (30). (geogise.com)
  • Sixty percent of cases express 1 or more T-cell antigens (CD3 + , CD43, or CD45RO). (medscape.com)
  • BCR-mediated antigen uptake can influence antigen processing and the nature of MHC-displayed T-cell determinants. (hindawi.com)
  • This antibody has been reported to stain RAW264.7 cell line. (thermofisher.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. (biolegend.com)
  • Applications Reported: This X54-5/7.1 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. (abcam.co.jp)
  • Conversely, deficient levels of a single subclass of IgG may be found in individuals who have effective specific antibody production and are clinically normal. (medscape.com)
  • The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions. (biolegend.com)
  • The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody. (bdbiosciences.com)
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (thermofisher.com)