• FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, whereas CISH probes are labelled with biotin or digoxigenin and can be detected using a bright-field microscope after other treatment steps have been applied. (wikipedia.org)
  • Only a few CISH probes are available commercially, so for most applications they have to be extracted, amplified, sequenced, labelled and mapped from bacterial artificial chromosomes (BACs). (wikipedia.org)
  • Gene-specific cDNAs had been quantified using Quant-IT PicoGreen (Invitrogen) and utilized to create high particular activity cDNA probes (>4.0×109 cpm/μg) by random-prime labeling with [α-32P] dCTP (6000 Ci/mmol) using the DECAprime II kit and hybridized at 107 cpm/mL for 15 hours at 42 °C in ULTAhyb (Ambion). (researchdataservice.com)
  • If digoxigenin was used as a probe label, an anti-digoxigenin fluorescein primary antibody followed by a HRP-conjugated anti-fluorescein secondary antibody are then applied. (wikipedia.org)
  • Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. (sigmaaldrich.com)
  • or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. (sigmaaldrich.com)
  • C-E) Co-labeling VMHvl neurons for PR (anti--gal) and Cckar (mRNA) in PRPL/PL mice. (cdc.gov)
  • Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible. (sigmaaldrich.com)
  • F) The vast majority of -gal+ (PR+) cells in various regions in PRPL mice are neurons, as evidenced by co-labeling with NeuroTrace (fluorescent Nissl) in the arcuate and NeuN for the remaining regions. (cdc.gov)
  • This approach works by targeting a specific RNA sequence with a self-priming hairpin (SP) probe and promoting continuously repeated extension and nicking reactions to produce numerous trigger molecules, which could specifically bind to molecular beacons (MBs) and produce fluorescent signals. (bvsalud.org)
  • Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. (wikipedia.org)
  • Probe labelling can be carried out by using either random priming or nick translation to incorporate biotin or digoxigenin. (wikipedia.org)
  • It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. (sigmaaldrich.com)
  • Among the numerous available small molecule BH3 mimetics, ABT-737, a potent small molecule that binds to Bcl-2/Bcl-xL with high affinity, has anti-tumor activity in a wide variety of cancer cells. (oncotarget.com)
  • The dense distribution of PLAP-labeled projections is only observed in the AVPV of females, but sparsely labeled fibers occupy a similar volume in the male AVPV. (cdc.gov)
  • however, coronavirus- pneumonia associated with human deaths and person-to- es had not been a prime consideration in the differential person disease transmission. (cdc.gov)
  • Probe labelling can be carried out by using either random priming or nick translation to incorporate biotin or digoxigenin. (wikipedia.org)
  • If digoxigenin was used as a probe label, an anti-digoxigenin fluorescein primary antibody followed by a HRP-conjugated anti-fluorescein secondary antibody are then applied. (wikipedia.org)
  • If biotin was used as a probe label, non-specific binding sites must first be blocked using bovine serum albumin (BSA). (wikipedia.org)
  • The last procedure is routinely used in the indirect method of in situ PCR. (nih.gov)