• spindle
  • These observations have led to the proposal that animal cells have a 'spindle assembly' checkpoint for the metaphase-anaphase transition that monitors bipolar spindle organization. (biologists.org)
  • However, the existence of such a checkpoint is uncertain because perturbations in spindle organization can produce unattached kinetochores, which by themselves are known to delay anaphase onset. (biologists.org)
  • We conclude that animal cells do not have a checkpoint for the metaphase-anaphase transition that monitors defects in spindle architecture independent of the checkpoint that monitors kinetochore attachment to the spindle. (biologists.org)
  • For dividing cells in which spindle microtubule assembly is not experimentally compromised, we propose that the completion of kinetochore attachment is the event which limits the time of the metaphase-anaphase transition. (biologists.org)
  • Once formed, the metaphase spindle maintains a characteristic length of ~2 µm for ~15-20 min before anaphase, during which it remains fairly rigid and can rotate up to 90° to align along the mother bud axis ( Winey and Bloom, 2012 ). (cloudfront.net)