• assays
  • The detection of infectious diseases at an early stage is critical not only for directing infection control and public health interventions.Our group aims to develop assays that are clinically valuable for the detection and management of infections and cancers using proprietary PCR-based techniques. (a-star.edu.sg)
  • In the Molecular Probes™ imaging webinars module, our scientists will cover imaging techniques such as fluorescence microscopy, immunohistochemistry, in situ hybridization, high-content screening, laser capture microdissection, cell-based reporter assays, and small animal in vivo imaging (SAIVI). (thermofisher.com)
  • biologist
  • Two categories of macromolecules in particular are the focus of the molecular biologist: 1) nucleic acids, among which the most famous is deoxyribonucleic acid (or DNA), the constituent of genes, and 2) proteins, which are the active agents of living organisms. (wikipedia.org)
  • Mostafa Ronaghi (born 1968) is an Iranian molecular biologist, specializing in DNA sequencing methodology. (wikipedia.org)
  • spatial
  • Chemical imaging (as quantitative - chemical mapping) is the analytical capability to create a visual image of components distribution from simultaneous measurement of spectra and spatial, time information. (wikipedia.org)
  • Any material that depends on chemical gradients for functionality may be amenable to study by an analytical technique that couples spatial and chemical characterization. (wikipedia.org)
  • Dexter energy transfer is a short-range phenomenon that falls off exponentially with distance (proportional to e−kR where k is a constant that depends on the inverse of the van der Waals radius of the atom[citation needed]) and depends on spatial overlap of donor and quencher molecular orbitals. (wikipedia.org)
  • It improved the spatial resolution of photothermal AFM-based techniques from microns to circa 100 nm. (wikipedia.org)
  • Novel extensions of the original AFM-IR technique and earlier techniques have enabled the development of bench-top devices capable of nanometer spatial resolution, that do not require a prism and can work with thicker samples, and thereby greatly improving ease of use and expanding the range of samples that can be analysed. (wikipedia.org)
  • The comparatively large size of these probes also limited spatial resolution. (wikipedia.org)
  • Ye et al described a MEM-type thermal probe giving sub-100 nm spatial resolution, which they used for nanothermal analysis. (wikipedia.org)
  • chemists
  • And what has the chemists really excited, is that the first applications of the technique offer a glimpse into the contorted form of a human protein that is implicated in type II diabetes. (bio-medicine.org)
  • nucleic
  • Our expertise is in the development and application of nucleic acid probe technology to diagnose and monitor human diseases. (a-star.edu.sg)
  • Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING. (uams.edu)
  • ions
  • In metalloneurochemistry, he studies the molecular activity of metal ions in the brain and develops optical and MRI sensors for binding, tracking, and measuring metal ions as they interact with neurotransmitters and other biological signaling agents. (wikipedia.org)
  • Molecular oxygen, iodide ions and acrylamide are common chemical quenchers. (wikipedia.org)
  • Fibers
  • Where measurements must be made at many different locations, a number of probes may be connected to a single remotely-located measuring instrument by optical fibers, as in the on-line process control monitoring system disclosed in U.S. Pat. (google.com)
  • species
  • The use of a pulsed high-voltage electric discharge jet coupled with mass-selective REMPI has been shown as a sensitive and powerful technique to generate a rich variety of atomic and molecular species and to probe their electronic transitions with increased sensitivity. (rsc.org)
  • Chemical
  • This molecular-level understanding is critical to designing chemical processes using SCF solvents. (epa.gov)
  • Multivariate (multiple variables) calibration techniques (e.g., principal components analysis, partial least squares, or artificial neural networks) are often employed to extract the desired chemical information. (wikipedia.org)
  • This structure, supported in part by X-ray crystallography (considered usually to be a "definitive" analytical technique) of a chemical derivative of MLA performed by Maria Przybylska, was accepted as correct until the early 1980s. (wikipedia.org)
  • Initially used for novel research in specialized laboratories, chemical imaging became a more commonplace analytical technique used for general R&D, quality assurance (QA) and quality control (QC) in less than a decade. (wikipedia.org)
  • Chemical imaging techniques are critical to understanding modern manufactured products and in some cases is a non-destructive technique so that samples are preserved for further testing. (wikipedia.org)
  • The binding energies of the measured electrons are characteristic of the chemical structure and molecular bonding of the material. (wikipedia.org)
  • processes
  • Spectrophotometric techniques based on emission, absorption or scattering processes are frequently used for qualitative and quantitative analyses. (google.com)
  • FOS has the following fields of application: surface metrology, precise probe positioning, automatic surface characterization, automatic surface modification/stimulation, automatic manipulation of nanoobjects, nanotechnological processes of "bottom-up" assembly, coordinated control of analytical and technological probes in multiprobe instruments, control of atomic/molecular assemblers, control of probe nanolithographs, etc. (wikipedia.org)
  • Scanning
  • Feature-oriented scanning (FOS), also object-oriented scanning (OOS), is a method of precision measurement of surface topography with a scanning probe microscope in which surface features (objects) are used as reference points for microscope probe attachment. (wikipedia.org)
  • Feature-oriented positioning (FOP) is a method of precise movement of the scanning microscope probe across the surface under investigation. (wikipedia.org)
  • predictive
  • The use of high powered computer programs should allow us to assemble the molecular data into predictive paradigms relating to the individual organism's response to its surroundings. (issx.org)
  • bulk
  • In fact, second harmonic generation and sum frequency generation discriminate against signals from the bulk, implicitly labeling them as surface specific techniques. (wikipedia.org)
  • temperature
  • This also uses an "active" SThM probe, which acts as a heater as well as a thermometer, so as to inject evanescent temperature waves into a sample and to allow sub-surface imaging of polymers and other materials. (wikipedia.org)
  • Ramping the temperature of the probe, and thus the temperature of the small sample region in contact with it, allows localized thermal analysis and/or thermomechanometry to be performed. (wikipedia.org)
  • The Lancaster group obtained spectra by detecting the absorption of infrared radiation using a temperature sensitive thermal probe. (wikipedia.org)
  • including techniques that measure temperature measure thermal expansion use broadband sources. (wikipedia.org)
  • biological
  • If we evaluate the molecular revolution within the context of biological history, it is easy to note that it is the culmination of a long process which began with the first observations through a microscope. (wikipedia.org)
  • Quenching and dequenching upon interaction with a specific molecular biological target is the basis for activatable optical contrast agents for molecular imaging. (wikipedia.org)
  • reaction
  • Local solvent densities measured in SCF's will be used to determine empirically correct intermolecular potential functions, which will enable molecular modeling tools to predict solubilities and reaction dynamics in these fluids. (epa.gov)
  • orbitals
  • Because of the high frequency of the light, and the substantial charge and energy of emitted electrons, photoemission is one of the most sensitive and accurate techniques for measuring the energies and shapes of electronic states and molecular and atomic orbitals. (wikipedia.org)
  • synthesis
  • As part of this effort we realized that metal complexes might also function as templates or structure directing agents for the synthesis of new molecular sieves. (utdallas.edu)
  • Imaging
  • Blum G, Weimer RM, Edgington LE, Adams W, Bogyo M (2009) Comparative Assessment of Substrates and Activity Based Probes as Tools for Non- Invasive Optical Imaging of Cysteine Protease Activity. (wikipedia.org)
  • analyte
  • Despite their low volume, the present inventors have discovered that the blood drops may still be accurately analyzed for the presence of an analyte using lateral flow detection techniques. (google.ca)
  • Microdialysis is a minimally-invasive sampling technique that is used for continuous measurement of free, unbound analyte concentrations in the extracellular fluid of virtually any tissue. (wikipedia.org)
  • The direction of the analyte flow is determined by the respective concentration gradient and allows the usage of microdialysis probes as sampling as well as delivery tools. (wikipedia.org)
  • However, the recovery of water-soluble compounds usually decreases rapidly if the molecular weight of the analyte exceeds 25% of the membrane's molecular weight cutoff. (wikipedia.org)
  • The extraction efficiency is defined as the ratio between the loss/gain of analyte during its passage through the probe (Cin−Cout) and the difference in concentration between perfusate and distant sampling site (Cin−Csample). (wikipedia.org)
  • Microdialysis probes can consequently be calibrated by either measuring the loss of analyte using drug-containing perfusate or the gain of analyte using drug-containing sample solutions. (wikipedia.org)
  • method
  • A method used to identify multilocus DNA banding patterns that are specific to an individual by exposing a sample of the person's DNA to molecular probes and various analytical techniques such as Southern blot analysis. (dictionary.com)
  • Clear examples of data analysis feature, designed to assist the reader in their choice of analytical method. (whsmith.co.uk)
  • 7. The method of claim 1 , wherein the metering channel is formed using a microfabrication technique. (google.ca)
  • 8. The method of claim 7 , wherein the microfabrication technique includes laser ablation. (google.ca)
  • 9. The method of claim 7 , wherein the microfabrication technique includes applying a solvent treatment to the membrane. (google.ca)
  • An improved NMR probe and method are described which substantially improve the resolution of NMR measurements made on powdered or amorphous or otherwise orientationally disordered samples contained within a sample holder (46). (google.com)
  • This review focuses on the intrinsic charge transport in organic photovoltaic (PVC) devices and field-effect transistors (SAM-OFETs) fabricated by vapor phase molecular self-assembly (VP-SAM) method. (mdpi.com)
  • With this method, surface features (objects) are used as reference points for microscope probe attachment. (wikipedia.org)
  • FOP method may be used in bottom-up nanofabrication to implement high-precision movement of the nanolithograph/nanoassembler probe along the substrate surface. (wikipedia.org)
  • solvent
  • Each of these different types has a different extent of molecular ordering within the solvent matrix, from spherical micelles to larger cylinders, aligned cylinders and even bilayered and multiwalled aggregates. (wikipedia.org)
  • applications
  • Common incandescent or quartz halogen light bulbs are most often used as broadband sources of near-infrared radiation for analytical applications. (wikipedia.org)
  • PCR is now a common and often indispensable technique used in clinical and research laboratories for a broad variety of applications. (wikipedia.org)
  • analysis
  • An optical probe for conducting light scattering analysis is disclosed. (google.com)
  • The solution leaving the probe (dialysate) is collected at certain time intervals for analysis. (wikipedia.org)
  • Also, the first thermal probes were Wollaston wire devices that were developed originally for Microthermal analysis (in fact PTMS was originally considered to be one of a family of microthermal techniques). (wikipedia.org)
  • thermal
  • Both groups used a conventional Fourier transform infrared spectrometer (FTIR) equipped with a broadband thermal source, the radiation was focused near the tip of a probe that was in contact with a sample. (wikipedia.org)
  • Anderson took the different approach of using a conventional AFM probe to detect the thermal expansion. (wikipedia.org)
  • Thanks to a number of techniques embedded in FOS, the distortions caused by thermal drifts and creeps are practically eliminated. (wikipedia.org)
  • protein
  • This technique gives the researcher the ability to observe the differences in the phosphorylation states of any given protein. (wikipedia.org)
  • surface
  • Dual-polarization interferometry (DPI) is an analytical technique that probes molecular layers adsorbed to the surface of a waveguide using the evanescent wave of a laser beam. (wikipedia.org)
  • In particular, we were intrigued with the idea that one might be able to physically trap metal complexes in the oxide pores rather than immobilize the complexes by attachment to the surface of the molecular sieve. (utdallas.edu)
  • The role and contribution of the transport through delocalized states of redox active surface molecular aggregates of NTCDI are exposed and investigated. (mdpi.com)
  • Instead, a probe movement by surface features is only carried out from the start surface point A (neighborhood of the start feature) to the destination point B (neighborhood of the destination feature) along some route that goes through intermediate features of the surface. (wikipedia.org)
  • With multiprobe instruments, FOP approach allows the application of any number of specialized technological and/or analytical probes successively to a surface feature/object or to a specified point of the feature/object neighborhood. (wikipedia.org)
  • sample
  • A technique by which the DNA of an individual can be compared with that found in a sample or another individual. (dictionary.com)
  • Also, because the probe can contact the sample directly, sensitive optics can be eliminated. (google.com)
  • Then, the sample is transferred to a different polyacrylamide gel (the affinity-trap gel) where affinity probes are immobilized. (wikipedia.org)
  • Later, Richard Smalley modified the technique and used a UV laser to excite the sample, in order to measure the binding energy of electrons in gaseous molecular clusters. (wikipedia.org)
  • various
  • The term refers to various techniques, depending on whether the ionization energy is provided by an X-ray photon, an EUV photon, or an ultraviolet photon. (wikipedia.org)
  • The original technique dubbed AFM-IR that induced resonant motion in the probe using a Free Electron Laser has developed by exploiting the foregoing permutations so that it has evolved into various forms. (wikipedia.org)
  • consists
  • The microdialysis probe is designed to mimic a blood capillary and consists of a shaft with a semipermeable hollow fiber membrane at its tip, which is connected to inlet and outlet tubing. (wikipedia.org)
  • Today's most prevalent shape, the needle probe, consists of a shaft with a hollow fiber at its tip and can be inserted by means of a guide cannula into the brain and other tissues. (wikipedia.org)
  • fundamental
  • This relatively limited definition will suffice to allow us to establish a date for the so-called "molecular revolution", or at least to establish a chronology of its most fundamental developments. (wikipedia.org)
  • Research
  • The invention of an organic donor-acceptor structural analog of a silicon p-n junction in 1974 [ 1 ] marked the beginning of the intensive research in the field of molecular electronic devices. (mdpi.com)
  • In spite of amazing progress in food supply and nutritional science, and a striking increase in life expectancy of approximately 2.5 months per year in many countries during the previous 150 years, modern nutritional research has a great potential of still contributing to improved health for future generations, granted that the revolutions in molecular and systems technologies are applied to nutritional questions. (mdpi.com)
  • produces
  • However, APPI ionizes a broad range of compound classes and produces both protonated and molecular ion peaks, resulting in a complex mass spectrum. (wikipedia.org)