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  • gene
  • Now, genetics is a rapidly expanding specialty in which direct analysis of gene defects is possible, and the potential for correcting these defects is on the horizon. (glowm.com)
  • Among them two large LQTS families linkage analysis with markers spanning known LQTS genes was carried out to identify the specific gene for mutational analysis. (beds.ac.uk)
  • The present study was carried out as a continued investigation in the same cohort of patients plus 10 new LQTS families/patients enrolled since the earlier report, and focused on mutational analysis of the KCNQ1 gene in the new families/patients and the LQT2 gene, KCNH2 in the whole cohort. (beds.ac.uk)
  • Gene polymorphism between species is much more diverse than the intraspecific polymorphism of lactoferrin. (wikipedia.org)
  • genome
  • Double-stranded (ds) RNA viruses are a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve) and virion organization (T-number, capsid layers or turrets). (wikipedia.org)
  • genes
  • Examples of procedures that use oligonucleotides include DNA microarrays, Southern blots, ASO analysis, fluorescent in situ hybridization (FISH), PCR, and the synthesis of artificial genes. (wikipedia.org)
  • Study of polymorphism of genes that encode lactoferrin helps selecting livestock breeds that are resistant to mastitis. (wikipedia.org)
  • Major topics in molecular evolution concern the rates and impacts of single nucleotide changes, neutral evolution vs. natural selection, origins of new genes, the genetic nature of complex traits, the genetic basis of speciation, evolution of development, and ways that evolutionary forces influence genomic and phenotypic changes. (wikipedia.org)
  • occur
  • xDNA has a rise of 3.2Å and a twist of 32°, significantly smaller than B-DNA, which has a rise of 3.3Å and a twist of 34.2° xDNA nucleotides can occur on both strands-either alone (known as "doubly expanded DNA") or mixed with natural bases-or exclusively on one strand or the other. (wikipedia.org)
  • It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks (DSB). (wikipedia.org)
  • specificity
  • Several such enzymes have been discovered (including CEL I, T4 endonuclease VII, Endonuclease V, T7 endonuclease I). One of the commonly used enzymes is Surveyor nuclease (CEL II), which cleaves the 3′ side of both DNA strands with high specificity at sites of base substitution or insertion/deletion. (wikipedia.org)
  • Humans
  • Blastocystis is a protozoal, single-celled parasite that inhabits the gastrointestinal tracts of humans and other animals. (wikipedia.org)
  • molecule
  • This conformational change permits the fluorophore and quencher to be free of their tight proximity due to the hairpin association, allowing the molecule to fluoresce. (wikipedia.org)
  • In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then "invades" a similar or identical DNA molecule that is not broken. (wikipedia.org)
  • Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break. (wikipedia.org)
  • genetic analysis
  • These include TILLING (for Targeting Local Lesions IN Genomes), which can be thought of as a 'reverse' counterpart to what is probably the most common tool for traditional genetic analysis: chemical mutagenesis and screening. (wiley.com)
  • Identification requires equipment for genetic analysis that is common in microbiology laboratories, but not available to most physicians. (wikipedia.org)
  • common
  • They may have common ancestry with plant positive strand RNA viruses in supergroup 1 with potyvirus lineages, respectively A new clade (as yet unnamed) of six viruses infecting filamentous fungi has been reported. (wikipedia.org)
  • viruses
  • It is a double-stranded RNA non-enveloped virus The members of genus Orbivirus within the Reoviridae family are arthropod borne viruses and are responsible for high morbidity and mortality in ruminants. (wikipedia.org)
  • temperature
  • xDNA is more stable when compared to regular DNA when subjected to higher temperature, and while entire strands of xDNA, yDNA, xxDNA and yyDNA exist, they are currently difficult to synthesize and maintain.Experiments with xDNA provide new insight into the behavior of natural B-DNA. (wikipedia.org)
  • structures
  • They are helical structures containing quandine tetrads that can form from one, two or four strands. (wikipedia.org)
  • Depending on the direction of the strands or parts of a strand that form the tetrads, structures may be described as parallel or antiparallel. (wikipedia.org)
  • These structures, formed from two separate G-rich strands, are termed bimolecular quadruplexes. (wikipedia.org)
  • human
  • Their analysis indicates human lncRNAs show a bias toward two-exon transcripts. (wikipedia.org)
  • The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. (wikipedia.org)
  • base-pair
  • These methods are simple to run using standard laboratory techniques and equipment, and can detect polymorphisms, single base pair mismatches, and insertions and deletions at low frequencies. (wikipedia.org)
  • genetics
  • In the early 1980s, the field of genetics involved pedigree analysis, risk assessment, and rare circumstances in which a precise diagnosis could be made by chromosomal analysis or biochemical studies. (glowm.com)
  • Thus
  • Thus far, x-nucleobases have been added to strands of DNA using phosphoramidite derivatives, as traditional polymerases have been unsuccessful in synthesizing strands of xDNA. (wikipedia.org)
  • specific
  • The PCR reaction should be optimized to create a single, strong PCR band, as non-specific bands will increase the background noise of the assay. (wikipedia.org)
  • This entry represents various uracil-DNA glycosylases and related DNA glycosylases (EC), such as uracil-DNA glycosylase, thermophilic uracil-DNA glycosylase, G:T/U mismatch-specific DNA glycosylase (Mug), and single-strand selective monofunctional uracil-DNA glycosylase (SMUG1). (wikipedia.org)
  • TDG and MBD4 are strictly specific for double-stranded DNA. (wikipedia.org)
  • cell
  • The NBS1 protein is one substrate of the ataxia telangiectasia mutated (ATM) kinase, which signals all three cell cycle checkpoints after DNA double-strand break damage ( 13 ). (aacrjournals.org)
  • break
  • Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases also possess AP lyase activity that permits them to cut the phosphodiester bond of DNA, creating a single-strand break without the need for an AP endonuclease. (wikipedia.org)
  • After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. (wikipedia.org)
  • genes
  • Study of polymorphism of genes that encode lactoferrin helps selecting livestock breeds that are resistant to mastitis. (wikipedia.org)
  • Major topics in molecular evolution concern the rates and impacts of single nucleotide changes, neutral evolution vs. natural selection, origins of new genes, the genetic nature of complex traits, the genetic basis of speciation, evolution of development, and ways that evolutionary forces influence genomic and phenotypic changes. (wikipedia.org)
  • In other words, as a single fertilized egg cell - the zygote - continues to divide, the resulting daughter cells change into all the different cell types in an organism, including neurons, muscle cells, epithelium, endothelium of blood vessels, etc., by activating some genes while inhibiting the expression of others. (wikipedia.org)
  • protein
  • D49, 292- the X-ray structure analysis of the 18 kDa fragment of duck ovotransferrin, corresponding to the NII domain of the intact protein, was reported at a resolution of 2.3 A. In this structure, the Fe(III) cation binds to two tyrosine residues and the synergistic carbonate anion in an identical manner to that found in the intact protein. (jove.com)
  • The impact of parasite exposure on HIV-1 transcriptional/translational events is monitored by using single cycle pseudotyped viruses in which a luciferase reporter gene has replaced the Env gene while the effect on the quantity of virus released by the infected macrophages is determined by measuring the HIV-1 capsid protein p24 by ELISA in cell supernatants. (jove.com)
  • This homolog (originally thought to be, and often spoken of as, a single protein) is crucial in multicellular organisms, where it prevents cancer formation, thus, functions as a tumor suppressor. (wikipedia.org)
  • SDS-PAGE analysis indicates that it is a 53-kilodalton (kDa) protein. (wikipedia.org)
  • The conformational-change mechanism confers certain advantages, but it also has drawbacks: serpins are vulnerable to mutations that can result in serpinopathies such as protein misfolding and the formation of inactive long-chain polymers. (wikipedia.org)
  • The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. (wikipedia.org)
  • The mechanism for conformational conversion to the scrapie isoform is speculated to be an elusive ligand-protein, but, so far, no such compound has been identified. (wikipedia.org)
  • Specificity
  • Analysis of Histone Antibody Specificity with Peptide Microarrays Evan M. Cornett 1 , Bradley M. Dickson 1 , Scott B. Rothbart 1 1 Center for Epigenetics, Van Andel Research Institute This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications. (jove.com)
  • The enzyme recognizes all base substitutions and insertions/deletions, and cleaves the 3′ side of mismatched sites in both DNA strands with high specificity This assay has been used to identify and analyze mutations in a variety of organisms and cell types, as well as to confirm genome modifications following genome editing (using CRISPR/TALENs/zinc fingers). (wikipedia.org)
  • Several such enzymes have been discovered (including CEL I, T4 endonuclease VII, Endonuclease V, T7 endonuclease I). One of the commonly used enzymes is Surveyor nuclease (CEL II), which cleaves the 3′ side of both DNA strands with high specificity at sites of base substitution or insertion/deletion. (wikipedia.org)
  • The enzyme was purified from celery and was given the name CEL I. CEL I was shown to cut DNA with high specificity on both strands at the 3′ side of base-substitution mismatches, and can therefore be used in enzyme mutation detection methods to identify mutations and polymorphisms. (wikipedia.org)
  • mutation
  • Although the apparent level of redundancy in miRNA-mediated regulation would suggest that a single dysregulation or mutation in a cancer cell would be insufficient to result in a biological effect, several recent observations have pointed to altered miRNA regulation in human cancer ( 11 - 13 ). (aacrjournals.org)
  • Mutation rates for single nucleotide sites for most organisms are very low, roughly 10−9 to 10−8 per site per generation, though some viruses have higher mutation rates on the order of 10−6 per site per generation. (wikipedia.org)
  • Therefore, other non-sequencing based approaches to detect the mutation or polymorphisms are required. (wikipedia.org)
  • structures
  • In this method the isolated oligosaccharides serve as an input for HPLC analysis, which allows discrimination between various glycan structures according to the number of monosaccharide units comprising them, with a resolution of a single monosaccharide. (jove.com)
  • Expansion of the Crystallography Open Database (COD) and Statistical Analysis of Crystal Structures. (vu.lt)
  • No. MIP-25/2013) The COD project (abbreviated from the Crystallography Open Database , http://www.crystallography.net/ ) aims at collecting in a single open access database all organic, inorganic and metal organic structures (except for the structures of biological macromolecules that are available at the PDB. (vu.lt)
  • This technique has been widely employed in the studies of nucleic acids structures and the use of it to monitor conformational polymorphism of DNA has grown tremendously in the past few decades. (mdpi.com)
  • DNA may undergo conformational changes to B-form, A-form, Z-form, quadruplexes, triplexes and other structures as a result of the binding process to different compounds. (mdpi.com)
  • mRNA
  • Statistical analysis showed a significantly higher APEX1 mRNA level in HNC tumour tissue than in control samples. (docphin.com)
  • More particularly, the present invention relates to methods and devices for the microdissection and analysis of cellular samples which may be used in combination with a number of different technologies that allow for analysis of enzymes, MRNA and DNA from pure populations or subpopulations of particular cell types. (google.com)
  • subsequent
  • Combining our workflow with the study of amplification efficiency offers the chance for researchers to evaluate enormous amounts of NGS-generated data in great detail, improving confidence over the downstream analyses and subsequent applications. (biomedcentral.com)
  • different
  • Here we review the recent CD spectroscopic studies of the induction of DNA conformational changes by different ligands, which includes metal derivative complex of aureolic family drugs, actinomycin D, neomycin, cisplatin, and polyamine. (mdpi.com)
  • humans
  • Blastocystis is a protozoal, single-celled parasite that inhabits the gastrointestinal tracts of humans and other animals. (wikipedia.org)
  • Double-stranded (ds) RNA viruses are a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve) and virion organization (T-number, capsid layers or turrets). (wikipedia.org)
  • changes
  • These studies show that CD spectroscopy is a powerful technique to monitor DNA conformational changes resulting from drug binding and also shows its potential to be a drug-screening platform in the future. (mdpi.com)
  • A series of uncoating steps and conformational changes accompany cell entry and replication. (wikipedia.org)
  • greatly
  • Methods that have been recently developed for proteomic analysis have greatly improved the characterization of glycoprotein N-linked glycans. (jove.com)
  • advantages
  • However, CD spectroscopy offers significant cost and sensitivity advantages when compared with other analysis methods. (mdpi.com)
  • viruses
  • They may have common ancestry with plant positive strand RNA viruses in supergroup 1 with potyvirus lineages, respectively A new clade (as yet unnamed) of six viruses infecting filamentous fungi has been reported. (wikipedia.org)
  • It is a double-stranded RNA non-enveloped virus The members of genus Orbivirus within the Reoviridae family are arthropod borne viruses and are responsible for high morbidity and mortality in ruminants. (wikipedia.org)
  • bind
  • Despite this distinction TNA can still bind with complimentary DNA and RNA strands and therefore act as an alternative self-replicating entity. (blogspot.de)
  • methods
  • The present invention relates to methods and devices for the analysis of cellular samples. (google.com)
  • Here we describe microplate based methods for these procedures that allow the analysis of large numbers of samples within a short time frame. (jove.com)
  • These methods are simple to run using standard laboratory techniques and equipment, and can detect polymorphisms, single base pair mismatches, and insertions and deletions at low frequencies. (wikipedia.org)
  • reaction
  • The PCR reaction should be optimized to create a single, strong PCR band, as non-specific bands will increase the background noise of the assay. (wikipedia.org)
  • method
  • We describe here a simple method for the isolation and analysis of metabolically labeled N-linked oligosaccharides. (jove.com)
  • 7. The method of claim 1, wherein said polymorphisms are selected from the group consisting of 27 VNTR, T-786C SNP, and G894T SNP. (patentsencyclopedia.com)
  • Finally, we developed a method to assess the confidence level per genotype a-posteriori, which helps to decide which alleles and individuals should be included in any further downstream analyses. (biomedcentral.com)
  • study
  • One study suggested that TP53 codon 72 polymorphisms, MDM2 SNP309, and A2164G may collectively be associated with non-oropharyngeal cancer susceptibility and that MDM2 SNP309 in combination with TP53 codon 72 may accelerate the development of non-oropharyngeal cancer in women. (wikipedia.org)
  • A 2011 study found that TP53 codon 72 polymorphism was associated with an increased risk of lung cancer. (wikipedia.org)
  • specific
  • This entry represents various uracil-DNA glycosylases and related DNA glycosylases (EC), such as uracil-DNA glycosylase, thermophilic uracil-DNA glycosylase, G:T/U mismatch-specific DNA glycosylase (Mug), and single-strand selective monofunctional uracil-DNA glycosylase (SMUG1). (wikipedia.org)
  • applications
  • This suggests that there is a significant window for timing of fungicide applications between the end of foliar growth and snowfall for successful control of snow mold with a single fungicide application. (apsnet.org)
  • complex
  • For this, labeling and pulse-chase analysis protocols are used that are usually complex and give very low yields. (jove.com)
  • In vivo genomic footprinting analysis reveals that the complex Bradyrhizobium japonicum fixRnifA promoter region is differently occupied by two distinct RNA polymerase holoenzymes. (currentprotocols.com)