• gene
  • Now, genetics is a rapidly expanding specialty in which direct analysis of gene defects is possible, and the potential for correcting these defects is on the horizon. (glowm.com)
  • In the following years, the gene coding for the β chain of the high affinity IgE receptor was localised to this region and two polymorphisms in this gene have been shown to be associated with the atopic phenotype. (bmj.com)
  • Neither did we succeed in finding either of the two polymorphisms described, nor could we identify any other polymorphisms within the gene. (bmj.com)
  • We have tested for linkage and association of enhanced IgE responsiveness as well as clinical manifestations of atopy to the chromosomal region 11q13 and the presence of polymorphisms in the gene. (bmj.com)
  • Gene polymorphism between species is much more diverse than the intraspecific polymorphism of lactoferrin. (wikipedia.org)
  • genome
  • Double-stranded (ds) RNA viruses are a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve) and virion organization (T-number, capsid layers or turrets). (wikipedia.org)
  • occur
  • xDNA has a rise of 3.2Å and a twist of 32°, significantly smaller than B-DNA, which has a rise of 3.3Å and a twist of 34.2° xDNA nucleotides can occur on both strands-either alone (known as "doubly expanded DNA") or mixed with natural bases-or exclusively on one strand or the other. (wikipedia.org)
  • It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks (DSB). (wikipedia.org)
  • specificity
  • Several such enzymes have been discovered (including CEL I, T4 endonuclease VII, Endonuclease V, T7 endonuclease I). One of the commonly used enzymes is Surveyor nuclease (CEL II), which cleaves the 3′ side of both DNA strands with high specificity at sites of base substitution or insertion/deletion. (wikipedia.org)
  • genes
  • Examples of procedures that use oligonucleotides include DNA microarrays, Southern blots, ASO analysis, fluorescent in situ hybridization (FISH), PCR, and the synthesis of artificial genes. (wikipedia.org)
  • Study of polymorphism of genes that encode lactoferrin helps selecting livestock breeds that are resistant to mastitis. (wikipedia.org)
  • Major topics in molecular evolution concern the rates and impacts of single nucleotide changes, neutral evolution vs. natural selection, origins of new genes, the genetic nature of complex traits, the genetic basis of speciation, evolution of development, and ways that evolutionary forces influence genomic and phenotypic changes. (wikipedia.org)
  • Humans
  • Blastocystis is a protozoal, single-celled parasite that inhabits the gastrointestinal tracts of humans and other animals. (wikipedia.org)
  • chromosomal
  • In the early 1980s, the field of genetics involved pedigree analysis, risk assessment, and rare circumstances in which a precise diagnosis could be made by chromosomal analysis or biochemical studies. (glowm.com)
  • molecule
  • This conformational change permits the fluorophore and quencher to be free of their tight proximity due to the hairpin association, allowing the molecule to fluoresce. (wikipedia.org)
  • In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then "invades" a similar or identical DNA molecule that is not broken. (wikipedia.org)
  • Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break. (wikipedia.org)
  • genetic analysis
  • These include TILLING (for Targeting Local Lesions IN Genomes), which can be thought of as a 'reverse' counterpart to what is probably the most common tool for traditional genetic analysis: chemical mutagenesis and screening. (wiley.com)
  • Identification requires equipment for genetic analysis that is common in microbiology laboratories, but not available to most physicians. (wikipedia.org)
  • viruses
  • They may have common ancestry with plant positive strand RNA viruses in supergroup 1 with potyvirus lineages, respectively A new clade (as yet unnamed) of six viruses infecting filamentous fungi has been reported. (wikipedia.org)
  • It is a double-stranded RNA non-enveloped virus The members of genus Orbivirus within the Reoviridae family are arthropod borne viruses and are responsible for high morbidity and mortality in ruminants. (wikipedia.org)
  • temperature
  • xDNA is more stable when compared to regular DNA when subjected to higher temperature, and while entire strands of xDNA, yDNA, xxDNA and yyDNA exist, they are currently difficult to synthesize and maintain.Experiments with xDNA provide new insight into the behavior of natural B-DNA. (wikipedia.org)
  • structures
  • They are helical structures containing quandine tetrads that can form from one, two or four strands. (wikipedia.org)
  • Depending on the direction of the strands or parts of a strand that form the tetrads, structures may be described as parallel or antiparallel. (wikipedia.org)
  • These structures, formed from two separate G-rich strands, are termed bimolecular quadruplexes. (wikipedia.org)
  • human
  • Their analysis indicates human lncRNAs show a bias toward two-exon transcripts. (wikipedia.org)
  • The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. (wikipedia.org)
  • base-pair
  • These methods are simple to run using standard laboratory techniques and equipment, and can detect polymorphisms, single base pair mismatches, and insertions and deletions at low frequencies. (wikipedia.org)
  • Thus
  • Thus far, x-nucleobases have been added to strands of DNA using phosphoramidite derivatives, as traditional polymerases have been unsuccessful in synthesizing strands of xDNA. (wikipedia.org)
  • specific
  • The PCR reaction should be optimized to create a single, strong PCR band, as non-specific bands will increase the background noise of the assay. (wikipedia.org)
  • This entry represents various uracil-DNA glycosylases and related DNA glycosylases (EC), such as uracil-DNA glycosylase, thermophilic uracil-DNA glycosylase, G:T/U mismatch-specific DNA glycosylase (Mug), and single-strand selective monofunctional uracil-DNA glycosylase (SMUG1). (wikipedia.org)
  • TDG and MBD4 are strictly specific for double-stranded DNA. (wikipedia.org)
  • cell
  • The NBS1 protein is one substrate of the ataxia telangiectasia mutated (ATM) kinase, which signals all three cell cycle checkpoints after DNA double-strand break damage ( 13 ). (aacrjournals.org)
  • break
  • Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases also possess AP lyase activity that permits them to cut the phosphodiester bond of DNA, creating a single-strand break without the need for an AP endonuclease. (wikipedia.org)
  • After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. (wikipedia.org)