• genes
  • In an effort to determine genes that are expressed in mycelial cultures of Neurospora crassa over the course of the circadian day, we have sequenced 13,000 cDNA clones from two time-of-day-specific libraries (morning and evening library) generating ∼20,000 sequences. (genetics.org)
  • Using these sequences, the representation of genes among the morning and evening sequences, respectively, in most cases does not reflect their expression patterns over the course of the day. (genetics.org)
  • On the basis of these data we predict that a significant proportion of the expressed Neurospora genes may be regulated by the circadian clock. (genetics.org)
  • Approximately 1400 genes were sequenced, 70% from a cDNA library generated from dissected basal bulbs (containing the pharyngeal gland cells) and 30% from cDNA libraries generated from whole mixed stage nematodes. (brillonline.com)
  • Genes encoding transthyretin-like proteins were abundant in the bulb library and in situ hybridisation confirmed that one of these is expressed in the basal bulb. (brillonline.com)
  • Genes encoding a variety of proteases, which were shown using in situ hybridisation to be expressed in the gut, were also identified. (brillonline.com)
  • Highly expressed genes often exhibit a high degree of codon bias whereas lowly expressed genes often contain many alternative synonymous codons [ 5 ]. (pubmedcentralcanada.ca)
  • Analysis of these plastid-related genes is important since dinoflagellates are the only eukaryotes to have them in the nucleus [ 14 ]. (pubmedcentralcanada.ca)
  • Under heat stress, we find that 63 TUGs are differentially expressed (d.e.) at 25°C compared with lower, no-stress condition temperatures (4°C and 17°C). Approximately one-third of d.e. eelgrass genes were characteristic for the stress response of the terrestrial plant model Arabidopsis thaliana. (mpg.de)
  • GO analysis by libraries showed several genes that were present in only one library suggesting that their expression may be stressor specific. (usm.edu)
  • Some authors use the term "EST" to describe genes for which little or no further information exists besides the tag. (wikipedia.org)
  • and c) the C-terminal DNA-binding domain, ETS, which binds to the consensus DNA sequence, 5-GGAA/T-3 within a 9-to-10 bp sequence, in the target genes it regulates. (wikipedia.org)
  • These proteins bind specific DNA sequences, including the kappa-B motif (GGGACTTTCC), in the promoters and enhancer regions of several genes and viruses, including human immunodeficiency virus (HIV). (wikipedia.org)
  • Prediction of the coding sequences of unidentified human genes. (wikipedia.org)
  • proteins
  • Mass spectrometric analysis of androgen-regulated proteins in these cells identified the metastasis-suppressor gene NDKA/nm23, a finding that may explain a marked reduction in metastatic potential when these cells express a functional androgen receptor pathway. (garvan.org.au)
  • Nevertheless, the discovery that some HERV proteins may contribute to biological events has quickly generated interest in open reading frame (ORF) sequences. (plos.org)
  • The Syncytin-1 and Syncytin-2 envelope glycoproteins are encoded by full-length HERV sequences belonging to the HERV-W and HERV-FRD families, respectively and, through cell differentiation mechanisms, these proteins are presumably essential for human placentation (reviewed in ). (plos.org)
  • Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. (genes2cognition.org)
  • Using coimmunoprecipitation and mass spectrometry analysis, we identified 58 proteins that interact with NDRG1 in prostate cancer cells. (genes2cognition.org)
  • The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. (wikipedia.org)
  • Members of the ZAS family, such as ZAS2 (HIVEP2), are large proteins that contain a ZAS domain, a modular protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. (wikipedia.org)
  • clones
  • The cDNA clones and sequence information can be used for future functional analysis and for construction of microarrays for monitoring of environmental stressors in coastal waters using wild or caged grass shrimp. (usm.edu)
  • In 1979 teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of such in bacterial plasmids In 1982, the idea of selecting random or semi-random clones from such a cDNA library for sequencing was explored by Greg Sutcliffe and coworkers. (wikipedia.org)
  • sequenced 178 clones from a rabbit muscle cDNA library In 1991 Adams and co-workers coined the term EST and initiated more systematic sequencing as a project (starting with 600 brain cDNAs). (wikipedia.org)
  • cells
  • citation needed] CD34 is expressed in roughly 20% of murine hematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • ArgBP2 is highly abundant in cardiac muscle cells at sarcomeric Z-disc structures, and is expressed in other cells at actin stress fibers and the nucleus. (wikipedia.org)
  • In cells that already express high level of Oct3/4, exogenously transfected Oct3/4 will lead to the repression of Rex1. (wikipedia.org)
  • Nucleotide
  • The octamer (made of eight units) in this family of transcription factors is the DNA nucleotide sequence "ATTTGCAT", the etymology for the naming of the octamer transcription factor. (wikipedia.org)
  • dbEST
  • All the 343 EST sequences have been deposited in the dbEST GenBank under accession numbers JZ535802 to JZ536144. (mdpi.com)
  • protein
  • The assembled sequences were annotated by BLAST searches against the public protein database. (usm.edu)
  • To systematically investigate Dub function, we initiated a global proteomic analysis of Dubs and their associated protein complexes. (genes2cognition.org)
  • Approximately
  • The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
  • consensus
  • Among 1301 clusters, 343 were categorized according to Gene Ontology (GO) hierarchy and were compared to the GO mapping of A. thaliana tentative consensus sequences. (purdue.edu)
  • Preparation
  • The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
  • database
  • For ∼50% of the sequences (710 of 1431), significant matches to sequences in the National Center for Biotechnology Information database (of known or unknown function) were detected. (genetics.org)
  • often
  • For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)
  • short
  • Written within the hugely winning Methods in Molecular Biology™ sequence structure, chapters contain short introductions to their respective issues, lists of the required fabrics and courses, step by step, comfortably reproducible protocols, and Notes sections, which spotlight pointers on troubleshooting and fending off identified pitfalls. (cartyspoetryjournal.com)