BiomarkerProteinProteinsInhibitorCohort StudiesAlzheimer'sApoptosisGlycosylated peptidesBiomarkersMass SpectrometryPathwayMaturationSerumGlycoproteinExtracellularPrognosticGeneticMeasurementMarkersAPOEComponents2019GeneLevelsTissuePlasmaDiseaseStudySpecificDetectionBloodCellsActivityMeasuresMeta-analysisResearchResponsesData analysisJoint
Biomarker3
- Clusterin may be a biomarker of disease, as well as a therapeutic target to potentially prevent this disease. (medicalxpress.com)
- Glycosylation of Human Plasma Clusterin Yields a Novel Candidate Biomarker of Alzheimer's Disease. (ox.ac.uk)
- A biomarker score combining normalized data for both lubricin and clusterin peptides increased the model R2 to 0.079. (harvard.edu)
Protein15
- Clusterin is a heterodimeric protein that aids in the clearance of cellular debris and is involved with apoptosis. (wikipedia.org)
- During a study spanning nearly a decade, researchers at The Ohio State University College of Medicine, Houston Methodist Research Institute and Houston Methodist Cancer Center have linked the protein clusterin-for the first time-to many different facets of cardiometabolic syndrome risk through its actions in the liver. (medicalxpress.com)
- The study found that a particular extracellular matrix protein called clusterin, which is overproduced from the fat cells of obese patients, is strongly related to insulin resistance. (medicalxpress.com)
- Previously this protein clusterin has been mostly studied for its role in neurodegenerative diseases such as Alzheimer's but it now appears to have a broader role in human physiology and disease. (medicalxpress.com)
- The clusterin protein is a key component of the extracellular matrix , so this work opens up a new vista to understand the role of clusterin in inter-cellular crosstalk of tissue microenvironments of various diseases, including diabetes , cancer and neurodegeneration," said the co-corresponding author, Dr. Stephen T. C. Wong, professor and chair of the Department of Systems Medicine and Bioengineering at Houston Methodist. (medicalxpress.com)
- Moreover, it was proposed that Clusterin functions as an extracellular chaperon that stabilizes stressed proteins in a folding-competent state, and that the protein is involved in programmed cell death. (biovendor.com)
- RayBio® Human Immunoquantitative (PCR-Based) Clusterin-like protein 1 ELISA Kit for cell culture supernatants, plasma, and serum samples. (raybiotech.com)
- The RayBio ® Custom Human Clusterin-like protein 1 IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Clusterin-like protein 1 in serum, plasma, and cell culture supernatants. (raybiotech.com)
- This assay employs an antibody specific for Human Clusterin-like protein 1 coated on a 96-well PCR plate. (raybiotech.com)
- Standards and samples are pipetted into the wells and Clusterin-like protein 1 present in a sample is bound to the wells by the immobilized antibody. (raybiotech.com)
- Several selected protein targets were validated by Western blot analysis. (biomedcentral.com)
- 2.2 Regulation of Apoptosis-Related Protein Expression We then performed apoptosis protein array analysis. (biobender.com)
- Subsequently plasma samples from the baseline visit of 173 subjects in an observational OA cohort were tested by SRM for peptides from nine of these proteins: afamin, clusterin, cartilage oligomeric matrix protein, hepatocyte growth factor, kallistatin, insulin-like growth factor binding protein, acid labile subunit, lubricin, lumican, and pigment epithelium-derived factor. (harvard.edu)
- We leveraged brain tissues from cohorts that also have been profiled using other -omics modalities, including genomics and transcriptomics, to perform a multi-layer genomic, transcriptomic and proteomic analysis of the TMT AD protein network to better understand the relationships among these different data types in the context of AD. (emtherapro.com)
- After data processing and outlier removal, a total of 8,619 proteins were used to build a protein co-expression network using the weighted gene co-expression network analysis (WGCNA) algorithm 17 (Fig. 1b , Extended Data Fig. 1 and Supplementary Tables 2 - 4 ). (emtherapro.com)
Proteins4
- Background Clusterin (Clu) is a stress-responding proteins with multiple biological features. (biosemiotics2013.org)
- Alternatively the anti-apoptotic proteins clusterin was. (biobender.com)
- For E-MDA-MB- 231, down-regulation of catalase, paraoxonase-2 (PON2), clusterin, an inhibitor of apoptosis proteins (IAPs) and cell stress proteins validated the notion that E-cadherin re-expression enhances TZT anti-cancer efficacy. (eurekaselect.com)
- As part of a proteomic analysis of knee synovial fluid from normal and OA patients, differentially expressed proteins were identified that could represent potential biomarkers for OA. (harvard.edu)
Inhibitor2
- Moreover cytoprotection mediated by clusterin was partially abrogated by Akt inhibitor LY294002. (biosemiotics2013.org)
- RESULTS: The proteome analysis revealed differential abundance of circulatory complement-lysis inhibitor (CLI), also known as Clusterin (CLU). (figshare.com)
Cohort Studies1
Alzheimer's3
- Another defining prominent function of Clusterin is its induction in many severe physiological disturbances states including kidney degenerative diseases, prostate and vesicle carcinogenesis, ovarian cancer and several neurodegenerative conditions (Alzheimer's disease). (biovendor.com)
- Eight β64N glycoforms are significantly reduced in patients with high atrophy compared with those with low atrophy, which demonstrates the utility of clusterin isoforms as diagnostic and prognostic Alzheimer's disease (AD) markers. (ox.ac.uk)
- Statistical map of rs2829887 T association with fibrillar amyloid projected onto the medial and lateral surfaces of a standardized brain, based on joint analysis of Pittsburgh Compound B or Avid-45 standard uptake value ratio images from 114 cognitively normal subjects in the Arizona APOE and Alzheimer's Disease Neuroimaging Initiative cohorts. (jamanetwork.com)
Apoptosis1
- In the apoptosis detection analysis, TZ increased early apoptosis significantly in MDA-MB-231 and MCF-7 while TRAIL induced late apoptosis significantly in E-MDA-MB-231. (eurekaselect.com)
Glycosylated peptides2
Biomarkers2
- These results provide a novel and robust workflow suitable for rapid verification of specific clusterin glycoforms with utility as AD biomarkers. (ox.ac.uk)
- Partial R2 values showed clusterin FMETVAEK and lubricin LVEVNPK peptide biomarkers explains about 2 to 3% of the variability of ΔJSW, similar to that explained by age. (harvard.edu)
Mass Spectrometry3
- HCP-mass spectrometry (HCP-MS) analysis is an orthogonal approach to enzyme-linked immunosorbent assay (ELISA) and can be used to achieve greater coverage and knowledge of the HCP profile of a biopharmaceutical. (bioprocessintl.com)
- The glycosylation of clusterin from human plasma was comprehensively analyzed and characterized using mass spectrometry (MS)-based glycoproteomics analysis. (ox.ac.uk)
- Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we have determined the differences in the glycoforms associated at each of the different glycosylation sites in plasma clusterin obtained from subjects of low hippocampal atrophy (n = 13) and high hippocampal atrophy (n = 14). (ox.ac.uk)
Pathway3
- Ingenuity® Pathway Analysis was used to identify significantly affected pathways in the transcriptomic and proteomic data sets. (biomedcentral.com)
- Conclusions Clusterin/Akt signaling pathway is involved in GATA-4 mediated cytoprotection against hypoxia stress. (biosemiotics2013.org)
- AD and Parkinson's disease, ALS, schizophrenia) and complex statistical analyses (e.g. gene-wide/burden, pathway analyses). (cardiff.ac.uk)
Maturation1
- Due to its wide breath of tissue distribution many diverse physiological functions have been attributed to Clusterin including sperm maturation, membrane recycling, lipid transportation, tissue remodelling, complement inhibition and cell-cell or cell-substratum interactions. (biovendor.com)
Serum4
- Recent study demonstrates, that serum Clusterin level increases significantly in diabetic type II patients and in patients with developing coronary heart disease, or myocardial infarction. (biovendor.com)
- These data raise the possibility that elevated Clusterin levels in serum may represent a strong indication of vascular damage. (biovendor.com)
- In patients with systemic lupus erythematosus (SLE), reduced serum Clusterin levels correlated inversely with disease activity. (biovendor.com)
- Kropáčková T, Šléglová O, Růžičková O, Vencovský J, Pavelka K, Šenolt L. Lower serum clusterin levels in patients with erosive hand osteoarthritis are associated with more pain. (biovendor.com)
Glycoprotein2
- BACKGROUND: Clusterin/apolipoprotein J (CLU) is a ubiquitous expressed glycoprotein with cytoprotective properties capable to prevent myocardial injury in experimental studies. (kl.ac.at)
- Clusterin can be a multifunctional glycoprotein which can be widely distributed in lots of tissues and controlled by a number of environmental adjustments. (biosemiotics2013.org)
Extracellular1
- ApoE and clusterin cooperatively suppress Abeta levels and deposition: evidence that ApoE regulates extracellular Abeta metabolism in vivo. (jamanetwork.com)
Prognostic1
- Multivariate Cox regression analysis demonstrated no independent prognostic significance of stromal Annexin A2 expression. (cancerindex.org)
Genetic1
- Genome-wide analysis of genetic loci associated with Alzheimer disease. (jamanetwork.com)
Measurement1
- Another interesting observation obtained in rat model suggests that measurement of urinary Clusterin levels may be a useful clinical marker for the severity of renal tubular damage. (biovendor.com)
Markers1
- These cells are best visualized with immunostaining using the FDC markers CD21, CD35, R4/23, clusterin, and KiM4p. (wikipedia.org)
APOE1
- Analyses were adjusted for subject age, APOE ε4 genotype, and cohort membership. (jamanetwork.com)
Components1
- Further translational research involving mouse models is needed to learn more about how clusterin impacts each of the CMS components and whether administrating clusterin-inhibiting antibodies inhibits CMS. (medicalxpress.com)
20191
- David Bradley et al, Clusterin Impairs Hepatic Insulin Sensitivity and Adipocyte Clusterin Associates With Cardiometabolic Risk, Diabetes Care (2019). (medicalxpress.com)
Gene2
- During the study, researchers performed gene expression, correlation analyses and blood measurements in 54 obese and 18 lean patients undergoing elective surgery at Ohio State's Wexner Medical Center. (medicalxpress.com)
- Association between apolipoprotein E gene polymorphism and the risk of coronary artery disease in Chinese population: evidence from a meta-analysis of 40 studies. (cdc.gov)
Levels6
- Characteristically, FDCS have mircotubuloreticular structures (MTRS) and increased levels of intracellular clusterin. (wikipedia.org)
- Lowered Clusterin levels could be involved in the pathogeneses of SLE on account of decreased protective effects. (biovendor.com)
- Conclusions: Our results suggest that when combined, levels of peptides representative of clusterin and lubricin in plasma are as predictive of OA progression as age. (harvard.edu)
- Meta-analysis of these studies confirmed that the AD patients had significantly higher plasma tau and ptau181 levels compared with controls, and that the plasma tau and ptau181 could predict AD with high-accuracy area under curve of the Receiver Operating Characteristic. (biomedcentral.com)
- In this systematic review and meta-analysis, we set out to determine the normal range of plasma tau and phosphorylated tau 181 (ptau181) levels in healthy populations stratified by age and sex, and investigate the cut-off thresholds of plasma tau and ptau181 between AD patients and controls. (biomedcentral.com)
- Understanding the biological effects of AD risk factor polymorphisms in human brain often requires additional levels of analysis using other -omics approaches. (emtherapro.com)
Tissue2
- Univariate Cox regression analysis showed that Annexin A2 and S100A10 in stromal tissue correlated with shorter overall survival (OS). (cancerindex.org)
- Nevertheless another meta-analysis demonstrated that intracoronary infusion of bone tissue marrow-derived mononuclear cell (BMMNC) in individuals with AMI didn't enhance cardiac function on MRI-derived guidelines nor achieved it improve medical result [1]. (biosemiotics2013.org)
Plasma1
- Clusterin can bind to aggregated LDL in 6H05 human being plasma and. (biosemiotics2013.org)
Disease1
- Association between apolipoprotein E polymorphisms and premature coronary artery disease: a meta-analysis. (cdc.gov)
Study1
- The goal of the study was to learn data analysis using various big data tools. (github.com)
Specific1
- The strategies and rationale for implementing HCP-MS analysis will differ depending on the product, process, and other project-specific variables. (bioprocessintl.com)
Detection1
- Human ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fixed, and labeled according to the protocol in the TiterTACS In Situ Detection Kit (Catalog # 4822-96-K ) prior to colorimetric analysis. (rndsystems.com)
Blood1
- This collaborative research is shedding new light on the importance of clusterin on "cardiometabolic syndrome," which may eventually lead to developing new treatments for this life-threatening combination of diabetes, high blood pressure and obesity," said Dr. K. Craig Kent, dean of the Ohio State College of Medicine. (medicalxpress.com)
Cells2
- Fat cells increase clusterin production as they enlarge in obesity. (medicalxpress.com)
- Riaz MA, Stammler A, Borgers M, Konrad L. Clusterin signals via ApoER2/VLDLR and induces meiosis of male germ cells. (biovendor.com)
Activity1
- Furthermore ΔΨm was caspase and maintained 3/7 activity was reduced by 6H05 clusterin inside a concentration-dependent way. (biosemiotics2013.org)
Measures1
- Analysis was limited to descriptive statistics.Main Outcome Measures: Primary outcome was safety through 18 months after implantation assessed by serial eye examinations, structural and functional testing, and adverse events (AEs) recording. (stanford.edu)
Meta-analysis1
- One meta-analysis demonstrated that BMSCs attenuated infarct size development and added to myocardial regeneration leading to general improvement of center function [3]. (biosemiotics2013.org)
Research1
- Her research has encompassed GWAS, sequencing, large exome chip association studies, as well as addressing more complex phenotypic analyses (e.g. (cardiff.ac.uk)
Responses1
- The part of miRNA-based rules from the innate immune system responses is a present topic of analysis (11). (cancer-ecosystem.com)
Data analysis2
- harshbg/PUBG-Game-Data-Analysis: An Analysis of Player Unknown's Battle Grounds (PUBG) Game Data using Hive and Spark. (github.com)
- The project was done as part of Big Data Analysis class at the University of Texas at Dallas. (github.com)
Joint1
- Peptides representative of clusterin, lumican and lubricin showed statistically significant associations with joint space narrowing after adjustment for age and sex. (harvard.edu)