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  • absorption
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  • cells
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  • Cells that do not grow ( ampicillin is bacteriostatic but not entirely killing E.coli ), however, appear later when ampicillin is degraded by a transformant colony. (bio.net)
  • So, if the number of actual transformants is very low like it is in blunt-end ligations, one tends to plate larger part of the transformation mixture, thus increasing the number of the cells surviving the ampicillin background. (bio.net)
  • In my hands, plating more then an equivalent of 1 ml log-culture cells on one 90-mm plate always resulted in quickly appearing background colonies, no matter fresh or old ampicillin was used. (bio.net)
  • Cells and cost fact studies, ampicillin chlamydia assess shingles, determine costo or traditionally research group made. (tecnodidactica.pe)
  • extent
  • So, the background must be proportional to the portion of the transformation mixture plated on one plate AND to the extent of ampicillin dying-out due to aged preparation, old plates etc. (bio.net)
  • cost
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  • however
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