Amount of viral dna viral. Medical search. Frequent questions

Whether you are developing cell and gene therapies or viral vaccines, removing contaminating nucleic acids is an important part of viral production. (sigmaaldrich.com)
It's non-specific and cleaves DNA and RNA into smaller fragments, acting on both single stranded and double stranded nuclei acids as well as linear and circular forms of nucleic acids. (sigmaaldrich.com)
Once these nucleic acids become broken down, Benzonase ® endonuclease and small DNA fragments can be easily removed by tangential flow filtration. (sigmaaldrich.com)
Whether for developing viral vaccines, viral vectors for vaccine, cell and gene therapy, or oncolytic viruses, Benzonase ® endonuclease removes nucleic acid contamination from the final product to meet regulatory standards. (sigmaaldrich.com)
By removing nucleic acids from the sample, this reduces DNA fouling as DNA binds to the resins and ensures maximal binding capacity for virus during chromatography to get the best yields. (sigmaaldrich.com)
It also helps meet regulatory expectations by reducing the amount of free nucleic acids in the final product. (sigmaaldrich.com)
Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)
Isolation of cfDNA and viral nucleic acid is challenging because of its low abundance in typical samples. (thermofisher.com)
The viral nucleic acid can be single-or double-strand DNA or RNA. (biopharminternational.com)
The kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. (zymoresearch.de)
Direct inspection of nucleic acids-often called "molecular genetics" or "DNA diagnosis"-is an important tool in a number of clinical areas, including oncology, infectious disease, forensics, and the general study of pathophysiology. (mhmedical.com)

DNA is normally contained in the nucleus of a cell, and therefore cells use proteins like ZBP1 as an indicator of a viral infection. (wikipedia.org)
We used blot hybridization with a cloned subgenomic probe to quantitate viral DNA in blood leukocytes of 60 viremic patients (25 with solid organ transplants, 20 with AIDS, and 15 marrow recipients) who had different clinical manifestations of CMV infection. (jci.org)
The Penn researchers found evidence in the phage DNA of genes that could make host bacteria more resistant to antibiotics, for example, or more likely to cause a harmful infection. (infectioncontroltoday.com)
Chronic infection with viral hepatitis affects half a billion individuals worldwide and can lead to cirrhosis, cancer, and liver failure. (wjgnet.com)
Cette invention se rapporte à un procédé pour traiter précocement une infection à papillomavirus affectant un orifice corporel chez l'homme ou chez un animal, y compris un canal associé à un tel orifice, ce procédé consistant à appliquer un agent d'inactivation virale sur cet orifice et/ou sur ce canal en une quantité propre à inactiver une partie du virus infectant ledit orifice ou ledit canal. (gc.ca)
A method of treating early papillomavirus infection of an orifice of a human or animal body including any canal associated with an orifice, comprising applying a viral inactivation agent to the orifice and/or canal in an amount effective to inactivate a portion of the virus infecting the orifice or canal. (gc.ca)
Cytomegalovirus infection (a viral infection that can affect almost any organ system, especially the eyes. (i-sis.org.uk)
Although inhalation of toxic gases and particles is the major risk factor for COPD 3 , viral infections, particularly childhood infection, add to the risk of developing this condition 1 . (ersjournals.com)
These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
It also blocks the DNA supply of the virus into the nucleus, reducing the amount of cccDNA by 20-100 times.Potentially useful for the treatment of the SARS-CoV-2 (COVID-19) infection, acting as a potent inhibitor of the RdRp protein. (prosci-inc.com)
In an R&D setting, this becomes preferable if the same reagent is transfected (for example, CAS9 delivery vectors) since all the work is in the generation of the viral stock, not in the infection," Carstens says. (biopharminternational.com)
And these viral particles have to find an access to wounded skin or mucosa in another person, resulting in HPV infection. (cdc.gov)
The adaptive immune response is thought to be responsible for viral clearance and disease pathogenesis during HBV infection. (medscape.com)
The choice of [HA620 trade name] to treat antiretroviral-experienced patients with HIV-1 infection should be based on individual viral resistance testing or the treatment history of the patient. (who.int)

Z-DNA-binding protein 1, also known as DNA-dependent activator of IFN-regulatory factors (DAI) and DLM-1, is a protein that in humans is encoded by the ZBP1 gene. (wikipedia.org)
DLM1 encodes a Z-DNA binding protein. (wikipedia.org)

A) The double -stranded breaks induced on the genome by nucleases are repaired by a homologous recombination mechanism that occurs between genomic DNA and donor DNA (B) A large number of donor DNA molecules are produced by Algentech's patented replication system (REP), thus contributing to the effectiveness of homologous recombination. (prnewswire.com)
2) a donor DNA amplification vector that relies on a viral replication system. (prnewswire.com)
However, nothing is known about the ability of the N-specific CD8 + T cell immunity in controlling viral replication in the lungs, a major pathogenic signature of severe disease in humans. (nature.com)
At the same time points, viral replication extents in the lungs were evaluated. (nature.com)
The impaired viral replication matched with a reduced induction of Spike-specific CD8 + T lymphocytes. (nature.com)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
The triphosphate metabolite competes with thymidine for incorporation into viral DNA, thereby causing DNA chain termination and inhibiting the function of HBV DNA polymerase and reverse transcriptase, consequently blocking the viral replication. (prosci-inc.com)
The objective here is to prevent formation of a replication-competent viral particle," Carstens emphasizes. (biopharminternational.com)
The primary objective of these viral vector systems is to achieve the highest possible titers without forming replication-competent variants. (biopharminternational.com)
A DNA polymerase with reverse transcriptase activity: Genomic replication takes place through an intermediate RNA known as pregenomic RNA. (medscape.com)

The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification. (neb.com)
Taq DNA Polymerase is an enzyme widely used in PCR. (neb.com)
Hot Start Taq DNA Polymerase allows for greater amplification sensitivity and increased ease of reaction setup. (neb.com)
The following guidelines are provided to ensure successful PCR using New England Biolabs' Hot Start Taq DNA Polymerase. (neb.com)
Mg++ concentration of 1.5-2.0 mM is optimal for most PCR products generated with Hot Start Taq DNA Polymerase. (neb.com)
We generally recommend using Hot Start Taq DNA Polymerase at a concentration of 25 units/ml (1.25 units/50 μl reaction). (neb.com)
However, the optimal concentration of Hot Start Taq DNA Polymerase may range from 5-50 units/ml (0.25-2.5 units/50 μl reaction) in specialized applications. (neb.com)
Hot Start Taq DNA Polymerase does not require a separate activation step. (neb.com)
The QIAcuity One-Step RT-PCR Master Mixes for hydrolysis probe-based dPCR use QIAGEN's latest version of high-quality DNA polymerase. (qiagen.com)
Several methods have been devised recently for very sensitive detection of variola virus DNA and to distinguish this DNA from that of other orthopoxviruses, the most promising being analysis by polymerase chain reaction (PCR) of restriction fragment length polymorphisms, multiplex PCR and real-time PCR with fluorigenic probes. (who.int)
Polymerase chain reaction (PCR) studies revolutionized many aspects of molecular biology, and DNA diagnosis came to involve this technique. (mhmedical.com)
polymerase involved, and this in- macromolecules including DNA, 2012) . (who.int)

Porcine circovirus (PCV) is a small, nonenveloped virus with a single-strand circular DNA genome of 1.7 kb in the family Circoviridae [ 6 ]. (biomedcentral.com)
HC-2 can detect as little as 1 pg/mL of target DNA (5000 genome copies per test). (medscape.com)
This is important both from the viral genome [ 4 - 6 ]. (who.int)
Over the last 10 years, CDC scientists in DPDM developed laboratory techniques specifically to extract a sufficient amount of DNA for analysis from this tiny organism and created a new typing tool to compare the pathogen's genome. (cdc.gov)

The study, published in the online journal mBio last month, reveals that most DNA viruses on healthy human skin are viral 'dark matter' that have never been described before. (infectioncontroltoday.com)
The findings also clearly linked the skin virome to the skin microbiome: Most of the detected viral DNA appeared to belong to phage viruses, which infect and often take up long-term residence within bacteria. (infectioncontroltoday.com)
And when Grice and colleagues sequenced skin bacterial DNA from the same 16 subjects, they found that it often contained tell-tale marks--called CRISPR spacers--of prior invasion by the same phage viruses. (infectioncontroltoday.com)
Within this large shell is an unusually large amount of viral DNA coding for proteins and enzymes not typically found in viruses. (wcponline.com)
This gene, called the adenosine deaminase acting on RNA 1, or ADAR1, protects the body from large amounts of the virus, but invites it in if only a small number of viruses knock on the door, the scientists found. (livescience.com)
Eventually, however, cells began using DNA, while viruses predominantly began encoding genetic information in RNA. (livescience.com)
Not all viruses store their genetic information in RNA, some store them in DNA. (livescience.com)
Viruses are composed of small amounts of DNA or RNA, encapsulated by a protein coat, and may be enclosed in an envelope made of proteins, carbohydrates, and lipids. (biopharminternational.com)
3 According to the latest draft on regulatory guidance from the European Agency for Evaluation of Medicinal Products (EMEA), potential contaminants may be enveloped or nonenveloped, small or large, DNA or RNA, labile or resistant viruses. (biopharminternational.com)
Viral safety of licensed biological products must be assured by three complementary approaches: (i) thorough testing of the cell line and all raw materials for viral contaminants, (ii) assessing the capacity of downstream processing to clear infectious viruses, and (iii) testing the product at appropriate steps for contaminating viruses. (biopharminternational.com)
It was worth noting that our study revealed a high abundance of antibiotic resistance genes (ARGs) in viral genomes, suggesting that viruses were key reservoirs of ARGs in AS systems. (biomedcentral.com)
However, the roles of viruses and the diversity of the viral community are just beginning to be understood in WWTPs. (biomedcentral.com)
By infecting and lysing host cells, viruses can affect the composition of microbial populations and facilitate the transfer of organic matter from cells to the dissolved organic matter pool via the "viral shunt" [ 9 ], and when consumed by small phagotrophs, viruses facilitate the movement of organic matter into the classical food web, which is called the "viral sweep" [ 10 ]. (biomedcentral.com)
Viral vector systems are gutted viruses where the packaging signals (e.g., the genetic signals that target the wrapping into the viral particles) are fused to the genes of interest (the payload), and the remaining necessary elements are placed on separate genetic elements, often three or more, says Carsten Carstens, senior scientist, R&D, Agilent Technologies. (biopharminternational.com)
HPVs are actually, they are small nonenveloped DNA viruses belonging to big family of papillomaviridae . (cdc.gov)

The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. (sigmaaldrich.com)

After the cells produce virus, they are lysed to release the viral particles, DNA, and RNA. (sigmaaldrich.com)
Benzonase ® endonuclease intensifies downstream processes by increasing efficiency of these steps and results in higher recovery of viral particles. (sigmaaldrich.com)
Using optimized techniques for isolating virus-like particles (VLPs) from skin swabs, and for analyzing very small amounts of genetic material, the research team was able to focus their sequencing and analysis on viral DNA without entirely depending on databases. (infectioncontroltoday.com)
A combination of several antiretroviral agents, termed Highly Active Anti-Retroviral Therapy (HAART), has been highly effective in reducing the number of HIV particles in the blood stream (as measured by a blood test called the viral load). (i-sis.org.uk)
To better understand the potential for the airborne spread of influenza, we measured the amount and size of aerosol particles containing influenza virus that were produced by coughing. (cdc.gov)
the saliva might be a very important liquid for transmission, and it can transfer the viral particles to a new area of the same oral mucosa. (cdc.gov)
The humoral antibody response contributes to the clearance of circulating virus particles and the prevention of viral spread within the host while the cellular immune response eliminates infected cells. (medscape.com)

Previous mapping attempts used databases of known viral genes to recognize some of this viral genetic material amid all of the bacteria and human DNA. (infectioncontroltoday.com)
However, most of the detected DNA from the VLPs did not match viral genes in existing databases. (infectioncontroltoday.com)
He was tipped as a Nobel candidate for his work on viral oncogenes (genes causing cancer). (i-sis.org.uk)
This fact complicates DNA diagnosis of patients and screening for carriers of defects in specific genes. (mhmedical.com)
HBV-X protein: This acts as a transcriptional transactivator for many viral and host genes through interaction with various transcription factors. (medscape.com)

Biodistribution and viral shedding assays are two critical means by which such safety can be assessed. (absorption.com)
Biodistribution (BD) assays determine the amount of viral DNA/RNA in different cells and organs in an organism. (absorption.com)
For half of the subjects, viral plaque assays were performed on the nasopharyngeal swabs and cough aerosol samples to determine if viable virus was present. (cdc.gov)
The most dominant techniques are PCR viral detection and signal amplification DNA-based assays (Hybrid Capture II is the only FDA-approved technique at present). (medscape.com)

We compared self- and staff-collected swabs in terms of swabbing quality and detection of viral respiratory pathogens. (plos.org)
Nasal self-swabbing for identification of viral ARI pathogens proved to be equivalent to staff-swabbing in this population in terms of acceptance and pathogen detection. (plos.org)
Akmatov MK, Gatzemeier A, Schughart K, Pessler F (2012) Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens. (plos.org)
Initial direct viral detection is typically performed using an upper respiratory tract (URT) specimen. (medscape.com)
But the detection of specific viral targets may vary depending on the amount of viral DNA present in the sample. (powershow.com)
The QIAcuity One-Step Viral RT-PCR Kit, combined with the QIAcuity Digital PCR System and the QIAcuity Nanoplates, enables quantitative analysis of RNA targets for various applications, including but not limited to viral RNA detection. (qiagen.com)
Most downstream application methods for viral detection do not require DNase treatment during purification. (zymoresearch.de)
High-risk HPV DNA sequences are either absent or the HPV solution DNA levels are below the detection limit of the assay. (medscape.com)

Human β-actin DNA concentration was determined in the swabs as a quality control. (plos.org)
The amount of β-actin DNA per swab was higher in the self- than in the staff-collected swabs (p = 0.008). (plos.org)
A respiratory viral pathogen was detected in 31% (23/75) of staff- and in 35% (26/75) of self-collected swabs (p = 0.36). (plos.org)
[ 4 ] Swabs are typically placed in 1.5-3 mL of viral transport media (VTM) prior to transport to the laboratory. (medscape.com)

Looking to isolate genomic DNA? (thermofisher.com)

For example, a skin swab taken for analysis will contain mostly human and bacterial DNA, and only a tiny amount of viral genetic material--the proverbial needles in the haystack. (infectioncontroltoday.com)
TRI Reagent ® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples. (sigmaaldrich.com)
The technology could help quickly detect viral or bacterial infections during major outbreaks. (medlineplus.gov)
[ 6 ] The brush is placed into specimen transport medium (STM), which has preservatives to retard bacterial growth and retain the integrity of the DNA. (medscape.com)

Upon i.m. injection of DNA vectors expressing Nef mut -derivatives, nanovesicles containing antigens fused with Nef mut are released by muscle cells, can freely circulate into the body, and can be internalized by antigen-presenting cells (APCs). (nature.com)
Shedding is the dissemination of the viral vectors (e.g. (absorption.com)
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable? (biopharminternational.com)
Several virus types have been studied for use as viral vectors in gene therapy, including adenovirus, lentivirus, retrovirus, and adeno-associated virus (AAV). (biopharminternational.com)

HIV uses reverse transcriptase to convert its RNA into DNA (reverse transcription). (iapac.org)

and delivery means to deliver to the treatment cavity an effective amount of a liquid agent for inactivation of papillomavirus for application to the surfaces bounding said treatment cavity including said external vaginal wall surfaces adjacent to the external os. (gc.ca)
Apparatus according to claim 3 wherein said stem means is of a co-axial tubular structure defining respective passages for the fluid to inflate the balloon and for infusion of the viral inactivation agent. (gc.ca)
DNA/RNA Shield included for sample collection, inactivation and storage. (zymoresearch.de)

To learn more about optimizing Benzonase ® endonuclease for your process, see our application note " Optimization Strategy and Process Economics of DNA Digestion in Viral Vector Production for Gene Therapy " and data sheet " Optimization of Benzonase ® endonuclease use in virus purification . (sigmaaldrich.com)
QIAamp Viral RNA Kits simplify viral RNA purification from cell-free body fluids with fast spin-column, vacuum and plate centrifugation procedures. (qiagen.com)
Purification can be fully automated on the QIAcube Connect using the QIAamp Viral RNA Mini Kit or dedicated QIAamp Viral RNA Mini QIAcube Kit. (qiagen.com)
Invitrogen DNA purification kits provide easy, fast and safe methods for isolation of DNA fragments that yield exceptional results. (thermofisher.com)
Removing viral contaminants from animal cell-culture derived biologicals is a major challenge of downstream purification because it involves laborious and time-consuming techniques that result in increased manufacturing costs. (biopharminternational.com)
Quick -DNA/RNA Viral MagBead kit is designed for high-throughput purification of viral DNA and/or RNA from plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies and swab and fecal samples stored in DNA/RNA Shield. (zymoresearch.de)
The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. (gdsbio.com)

Both versions are prepared in the E. coli W3110 expression system and pose a minimal risk of viral contamination. (sigmaaldrich.com)
A single reagent that can be used for RNA, DNA and protein isolation that is free of any contamination. (sigmaaldrich.com)
Viral contamination is a risk to all biotechnology products derived from cell lines of human or animal origin. (biopharminternational.com)
These include higher purity, lower volumes, and markedly reduced risks of viral contamination. (inknowvation.com)

Whether you prefer organic reagents, filter columns, or magnetic beads, our products for Isolation of DNA fragments are designed for sensitive, scalable extraction and are compatible with a range of downstream applications. (thermofisher.com)

Familiar examples include immunochromatography, which tests for viral antigens such as influenza and coronavirus, and antibody testing, which checks for the presence of antibodies. (agc.com)
The amount of influenza viral RNA contained in the samplers was analyzed using quantitative real-time reverse-transcription PCR (qPCR) targeting the matrix gene M1. (cdc.gov)

If the level of hepatitis B virus (viral load) is high in pregnant women, they are often given antiviral drugs during the last trimester of pregnancy to prevent transmission of the virus from mother to child. (msdmanuals.com)
See also Pediatric Hepatitis A, Pediatric Hepatitis C, and Viral Hepatitis. (medscape.com)

DLM1 then binds to cytosolic Viral DNA using two Z-DNA-binding domains (Zα and Zβ) at its N-terminus along with a DNA binding domain (D3). (wikipedia.org)
Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. (qiagen.com)

A machine learning classifier using 33 host immune response mRNAs accurately distinguishes viral and non-viral acute respiratory illnesses in nasal swab samples. (cdc.gov)

The BD and persistence of a specific viral coat can be analyzed by engineering a fluorescent protein (e.g. (absorption.com)

Additionally, viral isolates resistant to these drugs can emerge in immunosuppressed individuals, and second-line drugs for such variants are frequently accompanied by adverse effects requiring medical supervision. (frontiersin.org)
It was previously noted that the Centers for Disease Control and Prevention held 451 viral isolates obtained from different continents and countries when smallpox was endemic.2 The current review and the studies reported at the meeting concentrated on some 50 isolates in the Russian collection that were not present in the American collection. (who.int)

NIH is developing new tools that can quickly detect small amounts of DNA. (medlineplus.gov)

Homogenization or lysis of the tissue sample in TRI reagent ® dissolves RNA, DNA and protein. (sigmaaldrich.com)
Viral lysis of host microbes and virus-mediated HGT can regulate the biogeochemical and nutrient cycles, thus affecting the performance of AS systems. (biomedcentral.com)

Viral respiratory pathogens were detected by multiplex RT-PCR (Seeplex RV15 kit, Seegene, Eschborn, Germany). (plos.org)
PCR testing is a powerful tool to detect viral pathogens. (powershow.com)

The silica membrane technology captures DNA fragments without the need for pH adjustment. (thermofisher.com)

Recent studies have identified conserved host response to viral infections in the blood. (cdc.gov)

Reducing the amount of HIV and increasing CD4 cells in your blood may help improve your immune system. (iapac.org)
They found in the cells without ADAR1, even a small amount of double-stranded viral RNA activated the immune system. (livescience.com)
In these cells, they found the threshold for activating the immune system's alarm bells is about 1,000 snippets of double-stranded viral RNA. (livescience.com)

Both inactivated and live-attenuated chimeric PCV1-2b vaccines induced a robust antibody responses, and significantly decreased microscopic lesion and lower viral loads in serum or superficial inguinal lymph nodes (SILN) compared with that in the nonvaccinated challenged group. (biomedcentral.com)
About half (54.3%) of the patients had elevated alanine aminotransfe- rase levels and detectable serum HBV DNA. (who.int)

We developed a CD8 + T-cell-based vaccine platform based on intramuscular (i.m.) injection of a DNA vector coding for antigens of interest fused at the C-terminus of a biologically inactive Human Immunodeficiency Virus (HIV)-Type 1 Nef protein (Nef mut ) having an unusually high efficiency of incorporation into EVs. (nature.com)
a) HPV vaccine (Gardasil) has been shown to contain viral DNA impurities that have been related to fatal adverse events (SAEs, Refs. (sanevax.org)
The virus stocks undergo testing to determine if a sufficient amount of virus is present in the eggs, and the CVV is analyzed to determine if it is suitable for vaccine development in terms of compliance with regulatory requirements. (cdc.gov)

2 It is essential to review both the short-and long-term consequences of viral contaminants existing in biopharmaceutical products. (biopharminternational.com)
3 To ensure maximum viral safety, the ICH Q5A regulatory guideline mandates that manufacturers of therapeutic biological products for human use implement adequate technologies in their manufacturing process and demonstrate the capability of their processes to remove or inactivate known or adventitious contaminants based on a process-specific virus clearance strategy. (biopharminternational.com)
During the evaluation of the manufacturing process for any medicinal product, including vaccines, the possibility of impurities and endogenous viral contaminants is always investigated. (sanevax.org)

Routine isolation of DNA fragments from agarose gel is made easier with DNA gel extraction kits from Thermo Fisher Scientific. (thermofisher.com)
High recovery of DNA fragments is critical to the success of the entire workflow. (thermofisher.com)
Invitrogen DNA gel extraction kits are designed to isolate DNA fragments from agarose gel in 15-30 minutes. (thermofisher.com)
Vaccines may contain residual amounts of viral or cellular DNA fragments (depending on the manufacturing process) that originate from the processes used for their manufacture and this is not considered a contaminant but a residual substance. (sanevax.org)
The findings of residual amounts of viral DNA fragments referred to in the question are an expected finding and data in the literature supports that the residuals are rapidly eliminated and do not result in any expression of protein. (sanevax.org)

HBV is a double-stranded DNA virus of the Hepadnaviridae family. (medscape.com)
Human papillomavirus (HPV) is a nonenveloped double-stranded DNA virus and member of the Papovaviridae family. (medscape.com)

More than 90 percent was what we call viral dark matter--it had features of viral genetic material but no taxonomic classification,' Grice says. (infectioncontroltoday.com)
However, our knowledge about the diversity of viral taxonomic groups and functional traits in global AS systems is still limited. (biomedcentral.com)
To address this gap, we investigated the global diversity and biogeography of DNA viral communities in AS systems using 85,114 viral operational taxonomic units (vOTUs) recovered from 144 AS samples collected across 54 WWTPs from 13 different countries. (biomedcentral.com)

QIAamp Viral RNA Kits are intended for molecular biology applications. (qiagen.com)

In order to precisely insert or modify a genetic sequence, existing technologies use nucleases that introduce specific breaks in the target DNA and then rely on the cellular machinery to complete the editing process. (prnewswire.com)
In most cases, it could be because the samples contain high amounts of protein or cellular debris. (zymoresearch.de)

Researchers in Hong Kong report that testing patient blood for DNA from Epstein-Barr virus during treatment for nasopharyngeal carcinoma effectively predicts clinical outcome. (sciencedaily.com)

High levels of circulating cytomegalovirus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients. (jci.org)

High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc . (zymoresearch.de)

ZBP1 recognizes DNA in the cytoplasm as an antiviral mechanism. (wikipedia.org)
The antiviral effect appeared similarly strong when the viral challenge was carried out 3 months after boosting, and associated with the persistence of N-specific CD8 + T-resident memory lymphocytes. (nature.com)

DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer). (zymoresearch.de)
To help prevent this in future preps, it's best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step. (zymoresearch.de)

The new Applied Biosystems MagMAX Pure Bind beads offer a high‑performing, affordable and sustainable magnetic bead solution for DNA cleanup. (thermofisher.com)

Still, cancer cells contain EBV genetic material, which leaks into the bloodstream and can be detected using DNA tests. (sciencedaily.com)
That means a larger number of nasopharynx cancer cells in the body would give rise to a larger amount of EBV genetic material in the blood circulation, and so the EBV DNA level is a marker of the extent of cancer. (sciencedaily.com)

From the abstract: 'Viral acute respiratory illnesses (viral ARIs) contribute significantly to human morbidity and mortality worldwide, but their successful treatment requires timely diagnosis of viral etiology, which is complicated by overlap in clinical presentation with the non-viral ARIs. (cdc.gov)

The mechanism is simple: the DNA fragment (donor DNA) carrying the genetic modification or sequence to be inserted has at its ends sequences homologous to the DNA strand to be modified. (prnewswire.com)

The DNA assay validation was described in the dossier and assessed by the CHMP. (sanevax.org)

Researchers know that the amount of EBV DNA found after treatment is a recognized prognostic marker of survival because residual detectable EBV DNA "implies incomplete killing of cancer and thus a poor prognosis," Chan said. (sciencedaily.com)
Although the manufacturing process of vaccines is strictly controlled and designed to minimise residual DNA to within acceptable limits, the presence of trace amounts of such residues is inevitable. (sanevax.org)
The results confirmed the sensitivity of the residual DNA method for samples from the manufacturing process. (sanevax.org)

QIAamp DNA Mini Kit (QIAGEN, tients on haemodialysis and continuous tutos de Salud, "Dr C G. Malbrán" Gmbh,) was used to obtain DNA from ambulatory peritoneal dialysis. (cdc.gov)

These findings provide important insights into the viral diversity, function, and ecology in AS systems on a global scale. (biomedcentral.com)
The findings do not support that Gardasil is contaminated with HPV DNA and have no impact on its benefit risk balance. (sanevax.org)

serial twofold dilutions of each primer against a constant amount of purified SARS-CoV RNA. (cdc.gov)

Earlier this year, data from three US states - New York, Nevada and Massachusetts - showed that when the amount of the virus found in a person was taken into account, up to 90 percent of people who tested positive could actually have been negative, as they may have been carrying only tiny amounts of the virus. (rt.com)
Quality of DNA isolated munodefi ciency virus-infected patients The 6.7% seroprevalence from a from negative PCR samples was test- during the 1997-1998 infl uenza season. (cdc.gov)
The test is very effective because the DNA of a virus or bacteria will break down in high heat, and it can be identified by which enzyme does this breaking. (powershow.com)
In this study, we constructed a recombinant Alphavirus Semliki Forest virus (SFV) replicon vector DREP to express EBOV GP and matrix viral protein (VP40). (frontiersin.org)
They then infected cells with either the functioning gene or the deleted gene with different amounts of a measles virus. (livescience.com)
The measles virus stores its genetic information in RNA instead of DNA. (livescience.com)
The HA and NA from the bird flu virus are either prepared synthetically or by using the viral RNA of the wild type virus, if it is available. (cdc.gov)
Though encephalitis still is rare, its incidence both in the United States and internationally is increasing along with a rise in viral diseases like herpes, West Nile disease, Japanese encephalitis virus, and tick-borne encephalitis virus, among other causes. (aacc.org)
Carstens points out that the greatest limitation of viral vector systems is their tropism, that is, the type of cells that a virus will naturally infect. (biopharminternational.com)
HBV is a DNA virus in the Hepadnaviridae family. (medscape.com)

The TRACKMAN Connected system guides researchers through QIAamp Viral RNA Mini or QIAamp 96 Viral RNA protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. (qiagen.com)
In addition, it gives other researchers a ready-made toolkit for such investigations--Grice and her colleagues even made available, with the paper's supplemental information, the algorithms they devised for the DNA sequence analysis. (infectioncontroltoday.com)
The question the researchers investigated is whether there is a way to identify patients with such a viral load before treatment is finished so that more aggressive therapy might be instituted. (sciencedaily.com)
In this study, researchers tested 108 patients with advanced stage cancer for EBV DNA before the start of treatment, after a month of therapy, and then within three months after completion of treatment, and matched these levels to outcomes two-years later. (sciencedaily.com)

It is postulated that this intermediate phenotype may play a major role in the remodelling of the airways in chronic obstructive pulmonary disease associated with persistence of adenovirus E1A DNA. (ersjournals.com)

The technique produces a large amount of donor DNA strands, thus multiplying the efficiency of modifications introduced: a technological leap that finds applications in crop improvement, gene therapy and synthetic biology. (prnewswire.com)
Each viral vector system is characterized by an inherent set of properties that affect its suitability for gene therapy or other specific applications, says Florence Vicaire, global gene therapy business development leader, Cytiva (formerly part of GE Healthcare). (biopharminternational.com)

Q5: Is it normal for the Viral RNA (DNA/RNA) Buffer to range in color between clear and yellow? (zymoresearch.de)
Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added? (zymoresearch.de)
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. (zymoresearch.de)

If the sequences of the 10-20 nucleotides at the ends of a region of DNA of interest (such as a portion of a gene) are known, then "primers" complementary to these sequences can be synthesized. (mhmedical.com)
When even a minute amount of DNA from a patient (eg, from a few leukocytes, buccal mucosal cells, or hair bulbs) is combined with the primers in a reaction mixture that replicates DNA-and after several dozen cycles are then performed-the region of DNA between the primers will be amplified exponentially. (mhmedical.com)

1. Is electrophilic or can be metabolical y activated to electrophiles does not alter the linear sequence of nucleotides (or bases) in the DNA, 2. (who.int)

Each cycle exponentially increases the amount of viral DNA in the sample). (rt.com)

How does it compare to the amount of aluminum in HPV vaccines? (sanevax.org)

We found that patients with undetectable EBV DNA mid-course through treatment had a greatly reduced risk of developing cancer recurrence two years after treatment, compared with patients with detectable EBV DNA," said the study's senior investigator, Anthony Chan, M.D., director of the Cancer Center at the Chinese University of Hong Kong. (sciencedaily.com)
They found that 94 percent of patients had detectable EBV DNA before therapy, but that it became undetectable in 54 percent of patients midway through treatment. (sciencedaily.com)
Conversely, they found that levels detected after four weeks of treatment correlated with detectable post-treatment amounts, with an almost threefold greater risk of cancer recurrence and threefold higher risk of distant metastasis at two years. (sciencedaily.com)

Recent developments have made it possible to produce commercially viable amounts of factor VIII using recombinant host/vector systems. (inknowvation.com)

ADAR1 and the protein that it codes for, protects the body from attacking itself by finding and unzipping double-stranded RNA, a genetic relative of DNA , into single strands. (livescience.com)

More than 100,000 samples may need to be re-tested after a second inquiry, which examined the lab's 'fundamentally flawed' automated DNA extraction method used from 2007 to 2016 - known as Project 13. (yahoo.com)
Which DNA gel extraction kit is right for you? (thermofisher.com)
TRI reagent ® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. (sigmaaldrich.com)

Anatomy18

Organisms74

Diseases16

Chemicals and Drugs68

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes62

Information Science3

Health Care2

Nucleic12

Infection14

Binding protein2

Replication10

Polymerase12

Genome4

Viruses15

Proteins1

Particles7

Genes5

Assays4

Detection8

Swabs4

Genomic1

Bacterial4

Vectors4

Reverse transcriptase1

Inactivation3

Purification7

Contamination4

Range of downstream applications1

Influenza2

Hepatitis2

Binds2

Swab1

Specific viral1

Isolates2

Small amounts1

Lysis2

Pathogens2

Silica membrane1

Infections1

Immune3

Serum2

Vaccine3

Contaminants3

Fragments5

Double-stranded DNA virus2

Taxonomic3

Molecular1

Cellular2

Nasopharyngeal1

Cytomegalovirus1

QPCR1

Antiviral2

Digestion2

Magnetic bead1

Genetic material2

Respiratory1

Mechanism1

Assay1

Residual3

QIAGEN1

Findings2

SARS-CoV1

Virus10

Researchers4

Chronic1

Gene Therapy2

Buffer3

Primers2

Nucleotides1

Exponentially1

Vaccines1

Detectable3

Vector systems1

Strands1

Extraction3