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  • dissociation
  • The addition with lithium ion to the protein caused a decrease of the amounts of ADPribosylation of Gi protein by IAP in human platelet membranes and weekened the inhibition'of adenylate cyclase activity by activated Gi-protein, though the dissociation of the three subunits did not change. (nii.ac.jp)
  • The treatment caused a decrease of the amounts of ADP-ribosylation of Gi protein by IAP in human platelet membranes, weekened the phospholipase C activity by activated Gi-protein in differentiated HL-60 cells and inhibited the dissociation of the three subunits induced by Mg2+ and GTPgamma S. Western blot showed no remarkable quantitative changes in Gi protein in the experimental conditions mentioned above. (nii.ac.jp)
  • Receptor activation catalyzes the exchange of GDP for GTP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. (wikipedia.org)
  • The ribosomes are exceedingly small granules associated with endoplasmic reticulatum or free floating in the cytoplasm.They are also found in nucieus,chloroplast and mitochondria.Resent studies have shown that association and dissociation of ribosomal subunits depends on Mg++ ion concentration. (enotes.com)
  • This helical insertion domain functions to sequester the guanine nucleotide at the interface with the GTP-binding domain and must be displaced to enable nucleotide dissociation. (wikipedia.org)
  • PMID
  • Vesicles have specific coat proteins (such as clathrin or coatomer) that are important for cargo selection and direction of transfer [ PMID: 15261670 ]. (ebi.ac.uk)
  • While clathrin mediates endocytic protein transport, and transport from ER to Golgi, coatomers primarily mediate intra-Golgi transport, as well as the reverse Golgi to ER transport of dilysine-tagged proteins [ PMID: 14690497 ]. (ebi.ac.uk)
  • For example, the coatomer COP1 (coat protein complex 1) is responsible for reverse transport of recycled proteins from Golgi and pre-Golgi compartments back to the ER, while COPII buds vesicles from the ER to the Golgi [ PMID: 11208122 ]. (ebi.ac.uk)
  • Coatomers reversibly associate with Golgi (non-clathrin-coated) vesicles to mediate protein transport and for budding from Golgi membranes [ PMID: 17041781 ]. (ebi.ac.uk)
  • Coatomer protein complex I (COPI)-coated vesicles are involved in transport between the endoplasmic reticulum and the Golgi but also participate in transport from early to late endosomes within the endocytic pathway [ PMID: 12893528 ]. (ebi.ac.uk)
  • G proteins and their receptors (GPCRs) form one of the most prevalent signalling systems in mammalian cells, regulating systems as diverse as sensory perception, cell growth and hormonal regulation [ PMID: 15294442 ]. (ebi.ac.uk)
  • The length of the G protein signal is controlled by the duration of the GTP-bound alpha subunit, which can be regulated by RGS (regulator of G protein signalling) proteins or by covalent modifications [ PMID: 11313912 ]. (ebi.ac.uk)
  • These fall into 4 main groups on the basis of both sequence similarity and function: alpha-S ( IPR000367 ), alpha-Q ( IPR000654 ), alpha-I ( IPR001408 )and alpha-12( IPR000469 ) [ PMID: 1902986 ]. (ebi.ac.uk)
  • activates
  • CD45 -mediated dephosphorylation of Src family tyrosine-protein kinases FYN and LCK activates these kinases. (bio-rad.com)
  • G-alpha q11 activates phospholipase C beta ( PLC-beta ). (bio-rad.com)
  • The carbohydrate-responsive element binding protein ( ChREBP ) is a transcription factor that activates the lipogenic genes in the liver in response to excess carbohydrate in the diet. (bio-rad.com)
  • Signaling molecules bind to a domain of the GPCR located outside the cell, and an intracellular GPCR domain then in turn activates a particular G protein. (wikipedia.org)
  • The G protein activates a cascade of further signaling events that finally results in a change in cell function. (wikipedia.org)
  • whereas
  • All of them are activated by activated G-alpha (s) subunits, whereas G-alpha (i) is capable to inhibite some forms Adenylyl cyclases , including Adenylyl cyclases I [ 2 ]. (bio-rad.com)
  • Farnesyltransferase catalyzes the chemical reaction farnesyl diphosphate + protein-cysteine ⇌ {\displaystyle \rightleftharpoons } S-farnesyl protein + diphosphate Thus, the two substrates of this enzyme are farnesyl diphosphate and protein-cysteine, whereas its two products are S-farnesyl protein and diphosphate. (wikipedia.org)
  • regulation
  • The results strongly suggested that the qualitative changes of Gi-proteins are physiologically and pathologically functional in the regulation of transmembrane signal transduction. (nii.ac.jp)
  • Regulation of G proteins by covalent modification. (ebi.ac.uk)
  • sequence
  • Binding of SRP to the signal sequence as it emerges from the ribosome creates a cytosolic targeting complex containing the nascent polypeptide chain, the translating ribosome, and SRP. (ucsf.edu)
  • PKA phosphorylates two physiologically important sites in the ChREBP , which is located near nuclear localization signal sequence (NLS), and within the basic helix-loop-helix site, resulting in inactivation of nuclear translocation of ChREBP and of the DNA-binding activity, respectively [ 1 ]. (bio-rad.com)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • Farnesyltransferase (FTase) adds a 15-carbon isoprenoid called a farnesyl group to proteins bearing a CaaX motif: a four-amino acid sequence at the carboxyl terminus of a protein. (wikipedia.org)
  • ribosome
  • Proteins synthesised on the ribosome and processed in the endoplasmic reticulum are transported from the Golgi apparatus to the trans-Golgi network (TGN), and from there via small carrier vesicles to their final destination compartment. (ebi.ac.uk)
  • First the ribosome, freely floating in the cytosol, begins to synthesize a protein. (enotes.com)
  • and the ribosome/nascent protein/SRP complex is targeted to the SR on the endoplasmic reticulum. (enotes.com)
  • Both subunits act as anchor for the ribosome/nascent chain/SRP complex. (enotes.com)
  • Once GTP is hydrolyzed, the SRP of the ribosome/nascent chain/SRP complex is free from that trio and also free from the SR located on the endoplasmic reticulum. (enotes.com)
  • amino
  • G protein alpha subunits are 350-400 amino acids in length and have molecular weights in the range 40-45 kDa. (ebi.ac.uk)
  • The alpha faces the cytoplasm (outside of the ER) with an amino terminal spanning the membrane. (enotes.com)
  • This protein is contains a conserved, 120 amino acid motif called the RGS domain. (origene.com)
  • This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. (wikipedia.org)
  • This protein contains a conserved, 120 amino acid motif called the RGS domain and a cysteine-rich region. (wikipedia.org)
  • These are all highly similar proteins, with only small amino acid changes causing the diversity of the voltage-dependent gating mechanism, channel conductance and toxin binding properties. (wikipedia.org)
  • There are four binding pockets in FTase, which accommodate the last four amino acids on the carboxyl-terminus of a protein. (wikipedia.org)
  • Only those with a suitable CaaX motif can bind ('C' is Cysteine, 'a' is an aliphatic amino acid, and 'X' is variable). (wikipedia.org)
  • The carboxyl-terminal amino acid (X) discriminates FTase's targets from those of the other prenyltransferases, allowing only six different amino acids to bind with any affinity. (wikipedia.org)
  • KPNB1
  • Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. (genecards.org)
  • KPNB1 (Karyopherin Subunit Beta 1) is a Protein Coding gene. (genecards.org)
  • Importin subunit beta-1 is a protein that in humans is encoded by the KPNB1 gene. (wikipedia.org)
  • UniProt
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • nuclear import
  • The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. (genecards.org)