The main breakdown products of the enzyme on alginate were disaccharides, trisaccharides, and tetrasaccharides, which demonstrated that this enzyme acted as an endo-type alginate lyase. (up.pt)
Characterization1
Title: Purification and characterization of a novel alginate lyase from a marine streptomyces species isolated from seaweed Abstract: Alginate, a natural polysaccharide derived from brown seaweed, is finding multiple applications in biomedicine via its transformation through chemical, physical, and, increasingly, enzymatic processes. (up.pt)
Oligosaccharides3
The enzyme guluronate-specific alginate lyase (EC 4.2.2.11, formerly called poly(α-L-guluronate) lyase) catalyzes the following process: Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-α-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and α-L-uluronate at their reducing end. (wikipedia.org)
AlyDS44 is a novel enzyme, with the potential for efficient production of alginate oligosaccharides with low degrees of polymerization. (up.pt)
The applicability of different enzymes such as glycosidases, glycosyltransferases, glycophosphorylases and glycosynthases have been de-scribed around the review article considering their advantages and disadvantages of these biocat-alysts in the stereoselective and regioselective synthesis of different types of glycosylated mole-cules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glyco-peptides and glycoproteins with a clear focus on food and pharmaceutical chemistry. (preprints.org)
Pseudomonas4
The persistence of chronic Pseudomonas aeruginosa lung infections in cystic fibrosis (CF) patients is due to biofilm-growing mucoid (alginate-producing) strains. (scienceopen.com)
Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy. (scienceopen.com)
PelA deacetylase activity is required for Pel polysaccharide synthesis in Pseudomonas aeruginosa. (scienceopen.com)
Secretory alginate lyases from Pseudomoalteromonas elyakovii and from Pseudomonas aeruginosa were cloned by replacing the signal peptide of the alginate lyase with the eukaryotic signal peptide. (igem.org)
Microorganisms2
A collection of enzymes has been isolated from microorganisms and reported to function against the diverse polysaccharide constituents of seaweed cell wall, including alginate (Gao et al. (springeropen.com)
or most of the history of microbiology, microorganisms in these biofilms was polysaccharide. (cdc.gov)
Hydrolysis1
Oligomannuronic acids and oligoguluronic acids were prepared by enzymatic hydrolysis of alginate with alginate lyases. (maigrir-regime-musculation.com)
The systematic name of this enzyme class is alginate α-L-guluronate-uronate lyase. (wikipedia.org)
The optimal pH of this enzyme is 8.5 and the optimal temperature is 45¿C. It is a salt-tolerant alginate lyase with an optimal activity at 0.6 M NaCl. (up.pt)
We designed our device to leave no trace after the therapy has been completed , by initiating secretion of an alginate-degrading enzyme to break down the microcapsules followed by the apoptosis of therapeutic cells . (igem.org)
Pectate2
The tertiary structures of pectate lyases and rhamnogalacturonase A show a stack of parallel beta strands that are coiled into a large helix. (embl.de)
Three bacterial pectate lyases, a pectin lyase from Aspergillus niger, thestructures of rhamnogalacturonase A from Aspergillus aculeatus, RGase A,and the P22-phage tailspike protein, TSP, display the right-handedparallel beta-helix architecture first seen in pectate lyase. (embl.de)
Biofilm matrix2
In CF lungs, the polysaccharide alginate is the major part of the P. aeruginosa biofilm matrix. (scienceopen.com)
The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
Poly1
Other names in common use include alginase II, guluronate lyase, L-guluronan lyase, L-guluronate lyase, poly-α-L-guluronate lyase, and polyguluronate-specific alginate lyase. (wikipedia.org)
Complete genomic analysis has also been implemented to understand the genetic elements imparting the polysaccharide degradation ability of seaweed-associated bacteria (Sun et al. (springeropen.com)
We implemented microencapsulation of engineered human cells and designed the system for degradation of alginate microcapsules. (igem.org)
Degradation of alginate beads was demonstrated by alginate lyase from Sphingobacterium multivorum . (igem.org)
Schematic representation of alginate microcapsule and its degradation. (igem.org)
Enzymes1
Proteins containing these repeats most often are enzymes with polysaccharide substrates. (embl.de)
Residues1
Consecutive M or G residues form so called M- or G-blocks, whereas the alternating M and G units constitute MG-blocks of alginate (Duan et al. (igem.org)
Polymer2
A biofilm is a structured consortium of bacteria, embedded in a self-produced polymer matrix consisting of polysaccharide, protein and DNA. (scienceopen.com)
Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
Alginate microcapsules are an almost ideal solution for this problem: the encapsulated cells are protected against the cytotoxic cells while the material used is biocompatible and allows exchange of small molecules and proteins (Figure 1). (igem.org)
Amino2
The highly conserved regions of its amino acid sequences indicated that AlyDS44 belongs to the polysaccharide lyase family 7. (up.pt)
Chitosan is a one of the most abundant natural amino polysaccharides extracted from the exoskeleton of crustaceans and insect, from fungal cell walls, etc. (medcraveonline.com)
Sequences1
The lyases havehomologous sequences but RGase A and TSP do not show obvious sequencehomology either to the lyases or to each other. (embl.de)
Species1
The extraction, fractionation and HIV-1 inhibition potential of polysaccharides extracted from three species of marine sponges, Erylus discophorus , Cliona celata and Stelletta sp. (mdpi.com)
Analysis2
Polysaccharide substrate specificity analysis indicated that ALW1 could produce extracellular alginate lyase, laminarinase, fucoidanase and cellulase. (springeropen.com)
The genomic sequence analysis of strain ALW1 was chipped in to enrich our understanding of the genetic loci contributing to its capability of degrading diverse polysaccharides derived from seaweed cell wall. (springeropen.com)
Matrix1
Zobell (2) observed that the number of versibly associated (not removed by gentle rinsing) with a sur- bacteria on surfaces was dramatically higher than in the sur- face and enclosed in a matrix of primarily polysaccharide rounding medium (in this case, seawater). (cdc.gov)
Activity2
The anti-HIV activity of 23 polysaccharide pellets and three crude extracts was tested. (mdpi.com)
Metal ions Mn2+, Co2+, and Fe2+ increased the alginate degrading activity, but it was inhibited in the presence of Zn2+ and Cu2+. (up.pt)
Marine1
In this study a novel alginate lyase, AlyDS44, was purified and characterized from a marine actinobacterium, Streptomyces luridiscabiei, which was isolated from decomposing seaweed. (up.pt)
Structure1
Xanthan gum's chemical structure is distinct from those of host and dietary polysaccharides that are more commonly expected to transit the gastrointestinal tract, and little is known about its direct interaction with the gut microbiota, which plays a central role in digestion of other dietary fibre polysaccharides. (nature.com)
Production1
2020 ). These polysaccharides can be exploited as feedstock for biofuel production and utilized as supplies for food, fertiliser, cosmetic and pharmaceutical products after further processing (Aswathi Mohan et al. (springeropen.com)
Complex1
Processed foods often include food additives such as xanthan gum, a complex polysaccharide with unique rheological properties, that has established widespread use as a stabilizer and thickening agent. (nature.com)
Variety1
Seaweeds synthesise a wide variety of polysaccharides including alginate, agar, carrageenan, laminarin, fucoidan and cellulose, for use as cell wall components or storing nutrients (Jönsson et al. (springeropen.com)
Cell4
Alginate is the most widely used and clinicaly tested biomaterial for cell encapsulation. (igem.org)
Cell encapsulation is technically achieved by dropping a mixture of cells and liquid alginate into a calcium chloride solution which solidifies the droplets, transforming them into the hydrogel beads whose size in our system can be adjusted within the range of 300-500 μm in diameter. (igem.org)
In the next step of cell encapsulation, an outer alginate layer is applied. (igem.org)
2006). To allow cell growth and division within the alginate beads, the core of microcapsules is depolymerized with sodium citrate. (igem.org)