• EMSA
  • Isolate the DNA bound protein by EMSA: the DNA bound protein, migrated in acrylamide gel, is isolated by autoradiography as per an Electrophoretic mobility shift assay (EMSA) protocol. (wikipedia.org)
  • Historically, the experimental techniques of choice to discover and analyze DNA binding sites have been the DNAse footprinting assay and the Electrophoretic Mobility Shift Assay (EMSA). (wikipedia.org)
  • molecules
  • A sensor is an assembly required to detect and communicate a particular event: a device or biological structure which (i) recognizes an entity of interest (e.g., molecules, ions, or physical changes such as temperature) and (ii) transduces an event of recognition into a measurable signal. (frontiersin.org)
  • When attempting to identify biological molecules that would disrupt the formation of the F complex, researchers developed a high-throughput fluorescence polarization (FP)-binding assay. (wikipedia.org)
  • high
  • To determine the diagnostic and prognostic utility of high-sensitive troponin assays in very early phase of AMI (less than 3 hours since symptoms onset) by performing a meta-analysis of prospective studies. (jove.com)
  • Lack of biological assays to measure many of these contaminants in tissue specimens, the high cost of using these assays in large epidemiologic studies when they were available, and challenges related to the interpretation of the results when exposures were measured after the adverse reproductive event occurred limit the conclusions that can be drawn from these investigations. (thefreedictionary.com)
  • SAAB also allows the identification of sites with high relative binding affinity since the competition is inherent in the protocol. (wikipedia.org)
  • The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. (wikipedia.org)
  • biomolecules
  • Any change of the hydration shell of biomolecules due to changes in their structure/conformation results in a relative change of the movement along the temperature gradient and is used to determine binding affinities. (wikipedia.org)
  • enzymes
  • A continuous microplate assay for sirtuins and nicotinamide-producing enzymes. (mcw.edu)
  • Restriction enzymes, like transcription factors, yield a gradual, though sharp, range of affinities for different sites and are thus also best represented by PSFM. (wikipedia.org)
  • methods
  • Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. (elsevier.com)
  • The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. (elsevier.com)
  • This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology. (elsevier.com)
  • The development of minimum information standards of different methods is since 2008 being harmonized by "Minimum Information about a Biomedical or Biological Investigation" (MIBBI) project. (wikipedia.org)
  • Reactions
  • Essential aspects of binding assays include, but are not limited to, the concentration level of reactants or products (see radioactive section), maintaining the equilibrium constant of reactants throughout the assay, and the reliability and validity of linked reactions. (wikipedia.org)
  • selective
  • Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
  • Approaches
  • We propose the Minimum Information About a Cellular Assay (MIACA) for reporting a cellular assay, and CA-OM, the modular cellular assay object model, to facilitate exchange of data and accompanying information, and to compare and integrate data that originate from different, albeit complementary approaches, and to elucidate higher order principles. (wikipedia.org)
  • temperature
  • Besides the temperature logger, self-contained biological indicators that contain G. stearothermophilus spores can also be used for autoclave quality control test. (abnova.com)
  • tissues
  • MIAME contains a number of extensions to cover specific biological domains, including MIAME-env, MIAME-nut and MIAME-tox, covering environmental genomics, nutritional genomics and toxogenomics, respectively Electrophysiology is a technology used to study the electrical properties of biological cells and tissues. (wikipedia.org)
  • tissue
  • In addition to the bovine and porcine tissue from which pharmaceutical-grade heparin is commonly extracted, it has also been extracted and characterised from: Turkey Whale Dromedary camel Mouse Humans Lobster Fresh water mussel Clam Shrimp Mangrove crab Sand dollar Atlantic salmon Zebra fish The biological activity of heparin within species 6-11 is unclear and further supports the idea that the main physiological role of heparin is not anticoagulation. (wikipedia.org)
  • synthetic
  • Not only does the synthetic molecule prevent the association between the two initiation factors, but by binding to a different portion of eIF4E via the same motif, it has been shown to actually increase the binding affinity of eIF4E for endogenous (originating within an organism) 4E-BP1. (wikipedia.org)
  • It includes synthetic and computational aspects of the study of existing drugs and agents in development in relation to their bioactivities (biological activities and properties), i.e., understanding their structure-activity relationships (SAR). (wikipedia.org)
  • combines
  • The ApoTox-Glo™ Triplex Assay combines three Promega assay chemistries to assess viability, cytotoxicity and apoptosis within a single assay well. (abnova.com)
  • technology
  • This technology is known as Fab-TACS (Traceless Affinity Cell Selection) and is based on Strep-tagged Fab fragments, which reversibly capture and release the target cells. (wikipedia.org)
  • Through the development of RIA technology, researchers have been able to move beyond the use of radioactivity, and instead, use liquid- and solid-phase, competitive, and immunoradiometric assays. (wikipedia.org)
  • sequence
  • By combining the power of random-sequence selection with pooled sequencing, the SAAB imprint assay makes possible simultaneous screening of a large number of binding site mutants. (wikipedia.org)
  • detection
  • In this study, a comprehensive approach for the QC of a controversial herbal injection Shuang-Huang-Lian lyophilized powder (SHL) was established based on the quality fluctuation detection by a combination of CF and biological fingerprint (BF). (jove.com)
  • systems
  • Digoxigenin may be conjugated to sugars to study glycosylation events, even in biological systems. (wikipedia.org)
  • In biological systems, the analogous terms of host and guest are commonly referred to as enzyme and substrate respectively. (wikipedia.org)
  • The AhR-mediated activities of Cl-PAHs have been determined by using yeast assay systems. (wikipedia.org)
  • specific
  • Likewise, site-specific recombinases also show a varied range of affinities for different target sites. (wikipedia.org)
  • A specific class of Cl-PAHs, polychlorinated naphthalenes (PCNs), are persistent, bioaccumulative, and toxic contaminants that have been reported to occur in a wide variety of environmental and biological matrixes. (wikipedia.org)
  • measure
  • The first part of the assay simultaneously measures two protease activities: GF-AFC is used to measure cell viability, and bis-AAF-R110 to cytotoxicity. (abnova.com)