Affinity oligonucleotides. Medical search. Frequent questions

Therapeutic tiny or short modified antisense oligonucleotides (Tiny ASOs) can block gene expression or modulate splice-switching! (biosyn.com)
Aptamers 2016 will be co-hosted with our 2nd symposium on antisense and therapeutic oligonucleotides, Oligo 2016 , on 6th April 2016. (libpubmedia.co.uk)
Therapeutic oligonucleotides, such as antisense oligonucleotides or ribozymes, are short segments of DNA that have been designed to hybridize to a sequence on a specific mRNA. (justia.com)
1993. Inhibition of HIV-1 Replication in Cultured Cells with Antisense Oligonucleotides Encapsulated in Immunoliposomes. (justia.com)
Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)
Many oligo backbone modifications create unique binding capabilities and bolster sequence discrimination, making them useful for a variety of diagnostic and therapeutic applications such as SNP genotyping, antisense oligonucleotides, siRNA, and cell delivery. (biosearchtech.com)
Generate uncharged and nuclease-resistant oligonucleotide linkages- particularly useful for targeted cellular delivery of antisense therapeutic agents. (biosearchtech.com)
Materials and Methods CD39 and CD73 expression was suppressed using LNAplus TM antisense oligonucleotides (ASOs). (bmj.com)

Affinity Plus DNA & RNA Oligonucleotides are custom, single-stranded, and duplexed sequences that contain 1−20 locked nucleic acid nucleotides. (idtdna.com)
Modified nucleosides and nucleic acids, Oligonucleotide chemistry, developing covalently mercurated hybridization probes. (utu.fi)
In recent years the use of oligonucleotide based therapeutics derived from nucleic acids such as DNA or RNA, has increased significantly. (jascoinc.com)
Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
ASOs were synthesized as gapmers with flanking locked nucleic acids (LNA) to increase stability and affinity to the target RNA, leaving a central gap for recruitment of the RNA-degrading enzyme RNaseH I. Knockdown efficacy of ASOs on mRNA and protein level was investigated in primary human T cells. (bmj.com)

Phosphoryl guanidine oligo-2′-O-methylribonucleotides in neutral therapeutic oligonucleotides enable enhanced cell penetration! (biosyn.com)
Consequently, therapeutic oligonucleotides have tremendous potential for specificity of action (i.e. the down-regulation of a specific disease-related protein). (justia.com)
One well known problem with the use of therapeutic oligonucleotides having a phosphodiester internucleotide linkage is its very short half-life in the presence of serum or within cells. (justia.com)

A parallel development has been the preparation of imaging agents which can bind their targets with high specificity and affinity [ 10 ]. (hindawi.com)
Over the past decade, with the progress of molecular biology and radiochemistry, a variety of tracer with high specificity and affinity appeared. (hindawi.com)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
Such assays can be used to study binding affinity and recruitment specificity of either recombinant or cellular proteins [ 27 , 28 ]. (biomedcentral.com)

When generating synthetic oligonucleotides, including a terminal 3' or 5' phosphate requires alternate manufacturing steps to maintain or add the modification. (idtdna.com)
Synthetic oligonucleotides with covalently-attached CDPI3 have enhanced DNA affinity and have improved the hybridization properties of sequence-specific DNA probes. (genelink.com)

Electrophoretic mobility shift assays (EMSA) identified oligonucleotide-protein binding for risk and non-risk SNPs to nuclear extracts of A549, BEAS 2B, and IMR-90 lung cell lines. (cdc.gov)
Bound proteins were purified by DNA affinity precipitation assays (DAPA) and eluted proteins analyzed by mass spectrometry (MS), demonstrating that rs14798008 bound H1 histones. (cdc.gov)

Infoscitex will develop high affinity aptamers (synthetic DNA oligonucleotides) capable extremely high sensitivity detection of the pathogen (bacteria) or material deriving from the pathogen. (usda.gov)

My research focuses on the synthesis of modified nucleosides, oligonucleotides, metallated oligonucleotides, and their conjugation to develop hybridization probes. (utu.fi)

Oligonucleotide therapeutics are mainly based on artificial oligonucleotides that are chemically modified DNA or RNA, which improves their structural stability in the human body and affinity to their target molecules 1 ). (jascoinc.com)

In this dissertation, we examine the gas phase acidity and proton affinity of cytosine using Fourior transform mass spectrometry (FTMS), liquid chromatography mass spectrometry (LCMS) and ab initio calculations at B3LYP/6-31+G*. The experimental gas phase acidities and proton affinities were established using bracketing method, equilibrium method and Cooks kinetic method. (rutgers.edu)

Analysis with a seriesof mutated oligonucleotides defines a minimal single-stranded DNAPur-binding element. (embl-heidelberg.de)
Short CDPI3-oligonucleotides hybridize with single-stranded DNA to give more stable DNA duplexes than unmodified ODNs of similar length. (genelink.com)

Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)

Are you attaching the oligonucleotide to another molecule or surface? (idtdna.com)
All of these modifications can be used to link an oligonucleotide with another molecule or a surface. (idtdna.com)
A huge diversity of organic molecules can be synthesized efficiently where each individual molecule carries a unique oligonucleotide tag serving as amplifiable barcode. (novartis.com)
The oligonucleotide tag provides single-molecule detection by allowing amplification and sequencing for hit identification from in-vitro affinity screening of encoded libraries of immense diversity. (novartis.com)

We employed conditional translating ribosome affinity purification (TRAP) followed by RNA sequencing (TRAP-seq) for transcriptome profiling of DA neurons in transgenic Drosophila lines expressing human PARIS wild type (WT) or mutant (C571A). (nature.com)
In cell cultures, through RNA affinity purification, a process that isolates a single type of protein from a complex mixture, the team identified telomeric proteins (Shelterin components TRF1 and TRF2, and origin recognition complex subunits ORC1, ORC2, and ORC4) that bound to a TERRA oligonucleotide sequence but not to control oligonucleotides. (news-medical.net)

A methylated DNA affinity precipitation method was implemented to assay binding of proteins to methylated DNA. (biomedcentral.com)
Methylated DNA affinity capture by methyl-CpG binding proteins produces fractions highly enriched for methylated DNA, suitable for coupling to next generation sequencing technologies. (biomedcentral.com)

Nucleoside phosphate backbone modifications are useful for increasing the stability of oligonucleotides duplexes and expanding the library of potential conjugates. (biosearchtech.com)

A Systematic Evolution of Ligands by EXponential enrichment (SELEX) experiment begins in round one with a random pool of oligonucleotides in equilibrium solution with a target. (projecteuclid.org)
Oligonucleotide which displays specific binding to a protein or other target, often selected by an iterative cycle of affinity- based enrichment. (genomicglossaries.com)

Learn about our broad family of oligonucleotide modifications and get suggestions for selecting modifications that can help you in your research. (idtdna.com)
IDT offers hundreds of useful modifications for oligonucleotides. (idtdna.com)

DNA oligos up to 120 bases manufactured by an exclusive production process to minimize oligonucleotide crosstalk. (idtdna.com)

However, they do not simultaneously and directly measure the affinity parameters relevant to biological function and are biased towards strongly binding sequences. (nature.com)
Random priming is a type of primer extension in which a mixture of small oligonucleotide sequences, acting as primers, anneal to a heat-denatured double-stranded template. (enzolifesciences.com)

To synthesise a given set of genes, researchers design a set of shorter fragments (oligonucleotides), from which the genes can be assembled. (chemistryworld.com)

We report here the cloning and sequencing of a cDNA encodinga protein with strong affinity for the PUR element. (embl-heidelberg.de)

This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)
The method is based on a magnetic barcoding strategy: PCR-amplified target MTB genes are captured and magnetically labeled by a pair of complementary oligonucleotides conjugated to microspheres and magnetic nanoprobes. (cdc.gov)

The Hydrogen bonding is critical to DNA stability and reactivity of oligonucleotides. (rutgers.edu)

Both Avidin and Streptavidin possess four Biotin binding sites of very high affinity (Ka=1015 M−1). (justia.com)

The publication describes the preclinical activity of Secarna's high-affinity bimodal LNAplus™-based ASO, IM-T9P1, that simultaneously triggers toll-like receptor 9 (TLR9) signaling and downregulates programmed cell death ligand 1 (PD-L1) expression, a protein involved in the suppression of the adaptive immune system, in one molecular entity. (izb-online.de)

This can slightly increase affinity to streptavidin. (idtdna.com)

In of ion competition experiments, indicating that potassium, rubid- particular, the cryogenic temperatures used in all recent crys- ium, and cesium ions bind to the minor groove with similarly weak tallographic work may drastically shift the enthalpy-entropy affinity as sodium ions, whereas ammonium ion binding is some- balance of ion-water substitution. (lu.se)

Substituted 3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one nucleoside analogues have been synthesised from 5-alkynyl-uridine derivatives, incorporated into triplex forming oligonucleotides (TFOs) and found to selectively bind CG inversions with enhanced affinity compared to T. (port.ac.uk)

Different methods to identify the nature of the bound metal cofactor, the binding affinity and stoichiometry are presented. (bvsalud.org)

Choose from a wide variety of ReadyMade™ oligonucleotides for sample preparation, PCR, and gene expression analysis that are ready to ship upon order. (idtdna.com)
Finally, the amplified and selected oligonucleotides are pasted together to create the desired gene. (chemistryworld.com)

Data from a high throughput SELEX experiment consists of lists of thousands of oligonucleotides sampled after each round. (projecteuclid.org)
Thus far, SELEX experiments have been very good at suggesting the highest affinity oligonucleotide, but modeling lower affinity recognition site variants has been difficult. (projecteuclid.org)
We present a novel model, based on a biochemical parametrization of SELEX, which allows us to use data from all rounds to estimate the affinities of the oligonucleotides. (projecteuclid.org)
We use our model to analyze a SELEX experiment containing double stranded DNA oligonucleotides and the transcription factor Bicoid as the target. (projecteuclid.org)

Macrocyclic scaffolds are particularly attractive for designing selective G-quadruplex ligands essentially because, on one hand, they show a poor affinity for the "standard" B-DNA conformation and, on the other hand, they fit nicely with the external G-quartets of quadruplexes. (hindawi.com)

Cholesterol can be conjugated to oligonucleotides and can facilitate uptake into cells. (idtdna.com)

Over a few rounds, oligonucleotides having a high affinity for the target are selected. (projecteuclid.org)
2) Designing, synthesizing, and screening of a oligonucleotide-tagged library to increase our understanding of efficient target modulation. (novartis.com)

These studies have combined peptide affinity pull-downs and quantitative mass spectrometry enabled by SILAC ( S table I sotope L abeling by A mino Acids in C ell Culture) [ 32 ]. (biomedcentral.com)

Therefore, all IDT oligonucleotides are shipped without a terminal phosphate group unless requested. (idtdna.com)

IDT Azide modification uses an NHS ester functional group to attach an azide moiety at the 5', 3', or any internal position in an oligonucleotide. (idtdna.com)

For years IDT has been an industry leader in oligonucleotide production. (idtdna.com)

Within one reaction the backbone of the entire oligonucleotide is converted to the required form (e.g., thiophosphate-sugar backbone). (biosearchtech.com)

We offer a variety of high-quality custom and predesigned oligonucleotides suitable for PCR, cloning, next generation sequencing, CRISPR, and other applications. (idtdna.com)

With these 'selection strands', designed to have comparable binding affinities, a temperature step sorts the correct strands from those that suffered mutations or deletions. (chemistryworld.com)

can also be incorporated into oligonucleotides designed for custom applications. (idtdna.com)

A methylated DNA affinity resin can be applied to probe for MBD activity in extracts. (biomedcentral.com)

High-throughput sequencing-RNA affinity profiling (HiTS-RAP) 22 is conceptually similar to RNA-MaP, but uses the E. coli replication terminator protein Tus to stall the RNA polymerase after transcribing the variable section of the DNA library. (nature.com)

This natural 8-ring 24-membered macroheterocycle (Figure 2 ) displays high affinity for quadruplex and most importantly has no affinity for duplex DNA ( vide infra ). (hindawi.com)

3) Leveraging the results from oligonucleotide-encoded screening by synthesizing corresponding bioactive small molecules that can modulate protein function. (novartis.com)

Anatomy11

Organisms10

Chemicals and Drugs116

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes74

Disciplines and Occupations3

Technology, Industry, Agriculture4

Information Science3

Health Care2

Antisense8

Nucleic5

Therapeutic3

Specificity4

Synthetic oligonucleotides2

Assays2

Aptamers1

Synthesis1

Chemically modified1

Chromatography1

Single-stranded2

Probes1

Molecule4

Purification2

Proteins2

Conjugates1

Enrichment2

Modifications2

Oligos1

Sequences2

Fragments1

CDNA1

Complementary2

Stability1

Biotin1

Preclinical1

Streptavidin1

Ions1

Bind1

Bound1

Gene2

SELEX4

Conformation1

Cells1

Target2

Mass1

Terminal1

Functional1

Production1

Reaction1

High-quality1

Selection1

Custom1

Activity1

Library1

Displays1

Small1