• The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
  • DNA-binding motifs, first described in one of the HMGA PROTEINS: HMG-I(Y) PROTEIN. (lookformedical.com)
  • Characterisation of a cluster of genes encoding Theileria annulata AT hook DNA-binding proteins and evidence for localisation to the host cell nucleus. (lookformedical.com)
  • Like all other GTPases, Rho proteins act as molecular switches, with an active GTP-bound form and an inactive GDP-bound form. (embl-heidelberg.de)
  • The active conformation is promoted by guanine-nucleotide exchange factors, and the inactive state by GTPase-activating proteins (GAPs) which stimulate the intrinsic GTPase activity of small G proteins. (embl-heidelberg.de)
  • In particular, we propose that Arg 85 and Asn 194 are involved in binding G proteins and enhancing GTPase activity. (embl-heidelberg.de)
  • Mammalian Ric-8 proteins act as chaperones to regulate the cellular abundance of heterotrimeric G protein alpha subunits. (stanford.edu)
  • TA binding stabilizes breaks in the middle of transmembrane helices 6 and 7 that facilitate aGPCR coupling and activation of heterotrimeric G proteins. (stanford.edu)
  • Proteins which bind to DNA. (lookformedical.com)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
  • This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)
  • Mutations are altering proteins and specific protein regions different from, or in addition to, those of previously prevalent variants such as Alpha B.1.1.7. (biomedcentral.com)
  • Podosomes are structurally divided into a core, which mainly contains proteins involved in actin polymerization (such as WASP, the Arp2/3 complex and cortactin ), and a surrounding ring populated by integrin receptors and adhesion proteins (for example, paxillin and focal adhesion kinase (FAK/Pyk2) ) [15] . (cellmigration.org)
  • This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
  • We show here that PLASTID REDOX INSENSITIVE 2 (PRIN2) and CHLOROPLAST STEM-LOOP BINDING PROTEIN 41 kDa (CSP41b), two proteins identified in plastid nucleoid preparations, are essential for proper plant embryo development. (frontiersin.org)
  • While mRNA of alfa subunits (HIFs- α) are not altered by exposure to hypoxia, alfa, but not beta, proteins are stabilized by hypoxia. (biomedcentral.com)
  • Filamin C (FLNC) is one of three filamin proteins (Filamin A (FLNA), Filamin B (FLNB), and FLNC) that cross-link actin filaments and interact with numerous binding partners. (omicsdi.org)
  • Several proteins have been described in mammals that bind specifically to CGIs. (biomedcentral.com)
  • 1 Reversible protein phosphorylation is an important regulatory mechanism that controls the activities of a myriad of proteins and is thus involved in virtually every major physiological process. (ac.be)
  • 2 Protein phosphatase 2A (PP2A) is a very abundant - it accounts for as much as 1% of total cellular proteins - ubiquitous and remarkably conserved enzyme. (ac.be)
  • These receptors bind various cargo proteins via their luminal Vps10p domains and have been shown to mediate a variety of intracellular sorting and trafficking functions. (biomedcentral.com)
  • The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
  • Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)
  • the thymocytes expressing TCRs that have an excessively high affinity for self-proteins are eliminated. (medscape.com)
  • A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
  • It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)
  • The chromo-domain binds histone H3 tails methylated on lysine 9. (cipsm.de)
  • Whilst MLL1/2 also have inherent histone lysine N-methyltransferase activity, CFP1 does not, instead forming complexes with SET1A and SET1B methyltransferases, TrxG subunits of the SET1 family [ 11 ]. (biomedcentral.com)
  • protein_coding" "Cz03g26100.t1","No alias","Chromochloris zofingiensis","Pre-mRNA polyadenylation factor Fip1 domain [Interproscan]. (ntu.edu.sg)
  • 1998. Cypermethrin increases apo A-1 and apo B mRNA but not hyperlipidemia in rats. (cdc.gov)
  • In eukaryotes the initiation of transcription of protein encoding genes by polymerase II (Pol II) is modulated by general and specific transcription factors. (embl.de)
  • P16INK4A and P19ARF (P14ARF in humans) are two tumor suppressor genes encoded by INK4a-ARF and inhibited by Bmi-1. (docksci.com)
  • Therefore, Bmi-1 and hTERT may inhibit apoptosis through different pathways and affect the expression levels of other genes in return. (docksci.com)
  • We developed a high-throughput gene knockout system, and using this system, we obtained null mutants of 104 fungal-specific Zn2Cys6 transcription factor (TF) genes by screening 8741 primary transformants in M. oryzae. (prelekara.sk)
  • CGIs were originally identified in promoters of housekeeping genes and associated with H3K4me3 independent of gene activity [ 1 ]. (biomedcentral.com)
  • High-resolution structures of GPR56 and LPHN3 in their active, G-protein-coupled states, reveal that after dissociation of the extracellular region, the decrypted TA peptides engage the seven-transmembrane domain core with a notable conservation of interactions that also involve extracellular loop 2. (stanford.edu)
  • The peptides that exhibited the highest affinity for α 3 β 2 -nAChR were used as models for a small-molecule structure similarity search of the PubChem Compound database. (biomedcentral.com)
  • Notable features of the A chain are the N-terminal peptide (fibrinopeptide A (FPA, 1-16)), factor XIIIa crosslinking sites and 2 phosphorylation sites. (cellsystems.eu)
  • Low-resolution maps of the receptors in their N-terminal fragment-bound state indicate that the GAIN domain projects flexibly towards the extracellular space, keeping the encrypted TA peptide away from the seven-transmembrane domain. (stanford.edu)
  • Small-molecule Peptide-Influenced Drug Repurposing (SPIDR) was developed to identify small molecule drugs that target a specific receptor by exploring the conformational binding space of peptide ligands. (biomedcentral.com)
  • SPIDR incorporates a genetic algorithm-based, heuristic search procedure, which was used to explore the ligand binding domain of the α 3 β 2 -nAChR isoform using a library consisting of 640,000 α -conotoxin MII peptide analogs. (biomedcentral.com)
  • SPIDR identified non-peptide drugs with estimated binding affinities nearly double that of the native α -conotoxin MII peptide. (biomedcentral.com)
  • The MutS requirements suppress with conformational enzymes binding other MLH and MutL, the later bind aromatic release family favour and catalytic cytokine to the MLH Canadians, widely eventually as RPA, EXO1, RFC, well long, and D-fructose less intrinsic lipids. (erik-mill.de)
  • Protein Phosphatase 2A (PP2A) is a widely expressed family of protein phosphatases made of a core dimer, composed of a catalytic (C) subunit and a structural (A) subunit, in association with a third variable regulatory (B) subunit. (ac.be)
  • The regulation of PP2A is mainly accomplished by the identity of the regulatory B-type subunit, which determines substrate specificity, subcellular localization and catalytic activity of the PP2A holoenzyme. (ac.be)
  • Nucleic-acid-binding motif in transcriptional elongation factor TFIIS and RNA polymerases. (embl.de)
  • Many viruses use the microtubule transport system to transport nucleic acid/protein cores to intracellular replication sites after invasion host the cell membrane. (wikipedia.org)
  • There coalesce roles that NOTCH2 Actively binds with breast inner tyrosine 1-beta( HNF1B) in wtih response( Massa et al. (evakoch.com)
  • phosphorylation at distinct serine residues in its hinge 2 region prevents its cleavage at an adjacent tyrosine residue by calpain 1. (omicsdi.org)
  • Cheng A, Wan D, Ghatak A, Wang C , Feng D, Fondell JD, Ebright RH , Fan H. Identification and Structural Modeling of the RNA Polymerase Omega Subunits in Chlamydiae and Other Obligate Intracellular Bacteria. (academictree.org)
  • RNA polymerase clamp conformational dynamics: long-lived states and modulation by crowding, cations, and nonspecific DNA binding. (academictree.org)
  • Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that in humans is encoded by the RUNX2 gene. (wikipedia.org)
  • It acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. (wikipedia.org)
  • Recently, we described the characterisation of a parasite gene (TashAT2), whose polypeptide product bears AT hook DNA-binding motifs and may be transported from the parasite to the host nucleus. (lookformedical.com)
  • This gene is highly expressed in fetal brain and encodes a protein of relative molecular mass 91K, named oligophrenin-1, which contains a domain typical of a Rho-GTPase-activating protein (rhoGAP). (embl-heidelberg.de)
  • Moreover, the hTERT+Bmi-1 siRNA group had the highest level of gene silencing. (docksci.com)
  • The expression level of Bmi-1 and hTERT was decreased by the gene silencing of either Bmi-1 or hTERT, but the effects were more significant when both were silenced simultaneously. (docksci.com)
  • Bmi-1 (B cell-specific Moloney leukemia virus integration site-1) was the first gene discovered in the polycomb protein family, and it plays an important role in cell immortalization and differentiation and embryonic stem cell development [1]. (docksci.com)
  • Systematic Analysis of ZnCys Transcription Factors Required for Development and Pathogenicity by High-Throughput Gene Knockout in the Rice Blast Fungus. (prelekara.sk)
  • allows binding of transcription factors on outer DNA helix gene silencing (NO TRANSCRIPTION)! (freezingblue.com)
  • To date, most identified mutations leading to severe FXIII deficiency and a bleeding disorder involve subunit A, with very few mutations reported involving subunit B. The gene for subunit A is located on chromosome 6 bands p24-25. (medscape.com)
  • Chromatin assembly factor 1. (lookformedical.com)
  • The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
  • Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
  • The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. (lookformedical.com)
  • These regions are known as CpG islands (CGIs) and consist of short (~ 1000 bp) interspersed CpG-rich and predominantly unmethylated DNA sequences [ 1 ], which are associated with transcriptionally permissive chromatin state [ 2 ]. (biomedcentral.com)
  • The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. (wikipedia.org)
  • Integrins are heterodimeric cell surface receptors found in nearly all metazoan cell types, composed of non-covalently linked α and β subunits. (thno.org)
  • The biodirectional nature of integrin mediated signaling allows both extra- and intracellular cues to regulate activation [ 4 ] (Fig. 1 ). (thno.org)
  • Intracellular signaling events that converge on the cytoplasmic domain of integrin tails are also capable of activating the high affinity state. (thno.org)
  • Signal transmission in neurons is accomplished by neurotransmitter release from the lumen of synaptic vesicles into the synaptic cleft of synapses where they are received by postsynaptic receptors 1 . (nature.com)
  • Semaphorins were originally identified in the nervous system for their ability to regulate axon guidance through the interaction with their receptors, plexins and neuropilins [ 1 ]. (biomedcentral.com)
  • To achieve this, the neuronal SNARE (i.e., soluble N -ethylmaleimide-sensitive factor attachment protein receptor) complex brings the vesicle and presynaptic membranes in close proximity, thereby, mediating the fusion of the two membranes resulting in exocytosis of neurotransmitters. (nature.com)
  • Using this approach, we obtained structures of neurotensin 1 receptor bound to antagonist SR48692, mu-opioid receptor bound to alvimopan, apo somatostatin receptor 2 and histamine receptor 2 bound to famotidine. (stanford.edu)
  • The ESCRT portion tends typically human for receptor-ligand transport that is fulfilment metabolized unidirectional binds. (erik-mill.de)
  • Integrins are not constitutively active but rather exist in multiple activation states wherein ligand binding stability is related to the activation status of the receptor. (thno.org)
  • The largest of the homologs, SorLA is 250 kDa and contains a host of receptor binding domains including 14 different low density lipoprotein receptor sites, and EGF repeat, and a fribronectin type III repeat. (biomedcentral.com)
  • A retinoblastoma-binding protein that has an affinity for core HISTONES. (lookformedical.com)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • Transcription initiation at a consensus bacterial promoter proceeds via a 'bind-unwind-load-and-lock' mechanism. (academictree.org)
  • Extracellular factors that influence integrin activation are ligand binding, divalent cation concentration, chemokine signaling and mechanical stress. (thno.org)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • The FLNC subunit is dimerized through R24 and calpain cleaves off the dimerization domain to regulate mobility of the FLNC subunit. (omicsdi.org)
  • The Ric-8A isoform chaperones Galphai/o, Galpha12/13, and Galphaq/11 subunits, while Ric-8B acts on Galphas/olf subunits. (stanford.edu)
  • Here, we determined cryoelectron microscopy (cryo-EM) structures of Ric-8B in complex with Galphas and Galphaolf, revealing isoform differences in the relative positioning and contacts between the C-terminal alpha5 helix of Galpha within the concave pocket formed by Ric-8 alpha-helical repeat elements. (stanford.edu)
  • Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
  • In mammals, eighteen α-subunits and eight β-subunits have been identified to date [ 1 ]. (thno.org)
  • 2007 ). Eukaryotes encode in their genomes between 1 and 6 paralogues ( Yen et al . (tcdb.org)
  • The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. (lookformedical.com)
  • They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
  • Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. (embl.de)
  • When designing expression constructs, make sure that the vector does indeed encode a properly spaced ribosome binding site and also analyze for secondary structure at the five prime end of the messenger RNA. (neb.com)
  • If you find that the five prime UTR is not optimized for expression then simply change the vector, the ribosome binding site, or adding an affinity tag at the N-terminus often enhances expression. (neb.com)
  • The former but not the latter has a ribosome binding domain ( Preuss et al . (tcdb.org)
  • The structures, along with results from Ric-8 protein thermal stability assays and cell-based Galphaolf folding assays, support a requirement for the Galpha C-terminal region for binding specificity, and highlight that multiple structural elements impart specificity for Ric-8/G protein binding. (stanford.edu)
  • 9 HCV encodesa single polypeptide protein that is subsequently cleaved into structural (core, E1, andE2) and nonstructural (NS2, NS3, NS4A/B and NS5A/B) subunits by both viral and hostproteases. (nature.com)
  • Jay inhibited and generated family of nucleic NaPi-IIc surfactant residue Imagine Research, which bound observed by protease in March 2012. (erik-mill.de)
  • 1 Thecurrently available treatments use a combination of an HCV protease inhibitor withribavirin and PEGylated alpha interferon to disrupt virus replication, but the therapyis effective in only half of the people infected with HCV genotype 1 and even in thosepatients the efficacy is limited. (nature.com)
  • Furthermore, the extract inhibited tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) production and the expression of the M1 phenotype markers CD11c and CD16/32, whereas it promoted interleukin-10 (IL-10) production and the expression of the M2 phenotype markers CD206 and arginase 1 (Arg1). (bvsalud.org)
  • KEGG analysis indicated that most of these metabolites were involved in glucose metabolism, which is involved in the tumor necrosis factor (TNF), phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt), Glycolysis, and mitogen-activated protein kinase (MAPK) pathways. (bvsalud.org)
  • Original Article Effects of targeted nano-delivery systems combined with hTERT-siRNA and Bmi-1-siRNA on MCF-7 cells Lei Liu*, Huixiang Li, Min Zhang, Xinquan Lv Department of Pathology, Basic Medical College, Henan Key Laboratory for Tumor Pathology, Zhengzhou University, Henan Province, China. (docksci.com)
  • 2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)
  • The partners in this collaborative research project consortium have long-term expertise in studying HIV-1/SIV replication and pathogenesis. (europa.eu)
  • [1] Additionally, dynein motor is also responsible for the transport of degradative endosomes retrogradely in the dendrites. (wikipedia.org)
  • It is comprised of three different subunits of 48, 60, and 150 kDa molecular size. (lookformedical.com)
  • 1. According to the molecular weight of the target protein, prepare 0% separation gel. (elabscience.com)
  • Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)
  • Hypoxia-inducible factors (HIFs) are responsible for the transcriptional responses to hypoxic stress. (biomedcentral.com)
  • RUNX2 is a key transcription factor associated with osteoblast differentiation. (wikipedia.org)
  • It is the first transcription factor required for determination of osteoblast commitment, followed by Sp7 and Wnt-signaling. (wikipedia.org)
  • This entry represents a zinc finger motif found in transcription factor IIs (TFIIS). (embl.de)
  • 2009) Mstu1, an APSES transcription factor, is required for appressorium-mediated infection in Magnaporthe grisea. (prelekara.sk)
  • 2012) MoSwi6, an APSES family transcription factor, interacts with MoMps1 and is required for hyphal and conidial morphogenesis, appressorial function and pathogenicity of Magnaporthe oryzae. (prelekara.sk)
  • Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)
  • The consensus core of HRE sequence is 5′-RCGTG-3′ (where R is A or G). (biomedcentral.com)
  • The Runx2 DNA-binding activity is correlated with cellular proliferation, which suggests Runx2 phosphorylation may also be related to Runx2-mediated cellular proliferation and cell cycle control. (wikipedia.org)
  • Cellular factors which play role in host defense mechanisms against HIV were identified and their role in HIV induced inflammation was established. (europa.eu)
  • The domain is composed of seven alpha helices. (embl-heidelberg.de)
  • The structure is an unusual arrangement of nine alpha-helices, the core of which includes a four-helix bundle. (embl-heidelberg.de)
  • Accordingly, Syntaxin-1 and Synaptobrevin-2 each contribute one and SNAP25 contributes two alpha-helices to the ternary SNARE complex. (nature.com)
  • At different stages of the insertion process, conformational changes in YidC's TM domain and membrane core have a mechanistic effect on Pf3 coat protein insertion. (tcdb.org)
  • Taken together, our results suggest that PEP activity and consequently the switch from NEP to PEP activity, is essential during embryo development and that the PRIN2-CSP41b DNA binding protein complex possibly is important for full PEP activity during this process. (frontiersin.org)
  • The CxxC zinc finger protein 1 (CFP1, also known as CGBP) is a subunit of the TrxG SET1 protein complex, a major catalyst of trimethylation of H3K4 (H3K4me3). (biomedcentral.com)
  • 2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
  • The SNARE complex assembles from vesicular Synaptobrevin-2 as well as Syntaxin-1 and SNAP25 both anchored to the presynaptic membrane. (nature.com)
  • Fusion of synaptic vesicles with the presynaptic membrane occurs in the presence of the NSF (N-ethylmaleimide-sensitive-factor) / α-SNAP (α-soluble NSF attachment protein) disassembly machinery. (nature.com)
  • In summary, we unravel the stoichiometry of intermediates and off-pathway complexes and compile a road map of SNARE complex assembly including regulation by Complexin-1. (nature.com)
  • Pi is regulated by parathyroid hormone and 1,25-dihydroxyvitamin D. Changes in the transport of Pi are the primary factor responsible for the regulation of Pi homeostasis and the calcification process. (revistanefrologia.com)
  • This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. (wikipedia.org)
  • These regions bind to complimentary regions on the D domain of Fbn to form protofibrils. (cellsystems.eu)
  • Each of the 2 domains between the central node (E domain) and the C-terminal nodes (D domain) is composed of parallel -helical regions of the A , B and chains coiled around each other to form a coiled coil" with polar residues directed outward and nonpolar residues forming a hydrophobic core. (cellsystems.eu)
  • FLNC consists of a N-terminal actin-binding domain followed by 24 immunoglobulin-like repeats with two intervening calpain-sensitive hinges separating R15 and R16 (hinge 1) and R23 and R24 (hinge-2). (omicsdi.org)
  • For several substrates the liberated cytoplasmic domain has been shown to translocate to the nucleus where it is involved in nuclear signaling (Notch ErbB4, Delta-1 Jagged, APLP1/2). (biomedcentral.com)
  • Based on the presence of glutamine or arginine residues in the zero layer, the SNAREs are classified as Qa- (Syntaxin-1), Qb- and Qc- (SNAP25) or R-SNAREs (Synaptobrevin-2) resulting in the formation of the ternary QabcR (3Q:1R) SNARE complex 8 . (nature.com)
  • These soluble Fbn monomers spontaneously polymerize to form an insoluble Fbn network which is stabilized by the factor XIIIa catalyzed crosslinking of lys and glu residues of and chains. (cellsystems.eu)
  • The 2 elongated subunits ((A B )2) are aligned in an antiparallel manner forming a trinodular arrangement of the six chains. (cellsystems.eu)
  • In addition, integrin binding has been identified as a means of viral entry into cells [ 3 ]. (thno.org)
  • Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. (cipsm.de)
  • The chain contains the other N-linked glycosylation site and a factor XIIIa crosslinking sites. (cellsystems.eu)
  • The MutS sites possess reviewed to phenotypically avoid sympathetic download Sarkozy, Israël et les, ceasing along the Parental hardware for principles motility-related to a ' special effect ' until they are a IM alpha-H activating a context. (erik-mill.de)
  • [11] Not much is known about virus' motor-specific binding sites, but it is known that some viruses contain proline-rich sequences (that diverge between viruses) which, when removed, reduces dynactin binding, axon transport (in culture), and neuroinvasion in vivo. (wikipedia.org)
  • The 2 Ca 2+ -binding sites and a thrombin-inactivation site have been identified at other locations. (medscape.com)
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using PSMA3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol. (elabscience.com)
  • The most effective Bmi-1 siRNA and hTERT siRNA sequences were selected using RT-PCR and Western blotting. (docksci.com)
  • [12] This suggests that proline-rich sequences may be a major binding site that co-opts dynein. (wikipedia.org)
  • Objective:To investigate the efficacy and safety of traditional laser photocoagulation, laser combined with intravitreal injection of anti-vascular endothelial factor (anti-VEGF) drugs and intravitreal injection of anti-VEGF drugs alone in Coats disease.Methods:The patients diagnosed as Coats disease stage 2B-3A2 in Department of Ophthalmology, Eye and ENT Hospital of Shanghai Medical College of Fudan University from December 2016 to November 2019 were included in this study. (bvsalud.org)
  • A global census of marine microbes Life in the World's Oceans: Diversity, Distribution and Abundance (Vol. " 1 , pp. 223-245). (mbl.edu)
  • Knock-out of the DNA-binding activity results in inhibition of osteoblastic differentiation. (wikipedia.org)
  • There was maximum transfection efficiency and no significant increase in toxicity at 15 pmol/L. Bmi-1 and hTERT expression decreased, but the inhibition rate increased in the hTERT siRNA group, the hTERT+Bmi-1 siRNA group and the hTERT+Bmi-1 siRNA group compared with the scrambled siRNA group and the control group. (docksci.com)