• A sequence alignment of 15 representative [[GH13]] enzymes possessing a CBM58 reveals that the location of this domain is invariant as an extension of the B domain (Figure 2). (cazypedia.org)
  • however, as evidenced by genome sequence analysis, the organism lacks common enzymes required for the de novo synthesis of precursors of PGN, which rationalizes its MurNAc auxotrophy. (karger.com)
  • Several types of carbohydrate-active enzymes (CAZymes) may be used in glycosylation reactions. (researchsquare.com)
  • The genome sequence of Acremonium alcalophilum has revealed a large number of genes encoding biomass-degrading enzymes. (biomedcentral.com)
  • We identified a lipA gene in the genome sequence of Acremonium alcalophilum , encoding a protein with a predicted lipase domain with weak sequence identity to characterized enzymes. (biomedcentral.com)
  • Recently, the genome of A. alcalophilum was sequenced ( http://genome.jgi-psf.org/Acral2/Acral2.home.html ), providing a rich genetic resource for the identification of enzymes that potentially function under alkaline conditions. (biomedcentral.com)
  • Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. (omicsdi.org)
  • Amino acid sequence alignment from CLUSTALW (DNASTAR, Madison, WI). (cazypedia.org)
  • The structural integrity of the N-terminus is sensitive to changes in amino acid sequence surrounding N2 and possibly dependent on the presence of the carbohydrate moiety itself at N15 ( Tam and Moritz, 2009 ). (jneurosci.org)
  • In some embodiments, the mutated HPV L1 capsid protein has an amino acid sequence alteration that modifies in the subject HPV serotype-specific immunogenicity of the capsid protein relative to a naturally-occurring capsid protein. (justia.com)
  • Unusually, the predicted lipase displays ≈ 30% amino acid sequence identity to both feruloyl esterase and lipase of Aspergillus niger . (biomedcentral.com)
  • The amino acid sequence determines the characteristic folding patterns of the protein's structure, which is essential to enzyme specificity. (vidque.com)
  • By crystallographic, biophysical and in vivo approaches, we show that AfuABC is in fact a cyclic hexose/heptose-phosphate transporter with high selectivity and specificity for a set of ubiquitous metabolites (glucose-6-phosphate, fructose-6-phosphate and sedoheptulose-7-phosphate). (rcsb.org)
  • The Human Genome Project has revealed that protein-coding genes represent less than 2% of the total genome sequence [ 7 ], and the remaining greater portion of human genomes are regarded as "junk DNAs", for they do not encode any protein. (oncotarget.com)
  • The outcomes of the western blot and transcriptome sequencing tests were finally confirmed. (hindawi.com)
  • Additionally, the validation outcomes from western blotting and transcriptome sequencing demonstrated that OXEGs were differently expressed. (hindawi.com)
  • Additionally, the binding of the fluorophore to the antibody itself cannot interfere with the immunological specificity of the antibody or the binding capacity of its antigen. (wikipedia.org)
  • This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. (wikipedia.org)
  • This protein (XG-34) is a carbohydrate-bindning module evolved for specificity to a branched carbohydrate, non-fucosylated xyloglucan. (lth.se)
  • The protein, the first of an evolved derivative of CBM4-2, retains the overall fold of CBM4-2 from which is was derived despite its substantially modified specificity. (lth.se)
  • It also features a cleft, in this case lined by a phenylalanine and a leucine residue in place of the tryptophane and phenylalaine residues found in the wild type protein sequence (it carries an additional six modifications, most of which are found in the bindning site (as illustrated in the accompanying figure) among its 165 residues in comparison to the wild type module). (lth.se)
  • 2015). Determining the composition of protein, carbohydrate, moisture, fat, and ash in raw EBN is essential for various purposes, including the hydrolysis process for the extraction of its valuable components. (researchgate.net)
  • For reference, the CBM58 of SusG (BT_3698) extends from residues 216-335.Red, orange and green colors at the top indicate positions of complete conservation, similarity, or more diverse sequence respectively. (cazypedia.org)
  • AfuABC is conserved across a wide range of bacterial genera, including the enteric pathogens EHEC O157:H7 and its murine-specific relative Citrobacter rodentium, where it lies adjacent to genes implicated in sugar sensing and acquisition. (rcsb.org)
  • LncRNAs can also be classified as cis-acting lncRNAs, which regulate target genes at adjacent regions, and trans-acting lncRNAs, which function at independent chromosomal loci [ 8 ]. (biomedcentral.com)
  • Both flagellar and somatic (mostly carbohydrate) surface antigens contribute to the serotypic designation of the organism ( 36 ). (asm.org)
  • Genomic sequencing of A. fulgidus suggests that three [Ni-Fe]-CODHs are present, a "bacterial" monomeric CooS and two archaeal CdhAB-type CODHs [ 9 - 11 ]. (hindawi.com)
  • Transmission of Leishmania tropica was studied in 2 adjacent foci in Israel where vector populations differ. (cdc.gov)
  • In some of the earliest modifications, researchers used double-stranded DNA sequences to express the V H or V L domains, but none of the sequence of the constant region (see, e.g. (justia.com)
  • Here we used PCR testing, viral enrichment-based sequencing and agnostic metagenomic sequencing to analyse samples from 16 HAdV-positive cases from 1 October 2021 to 22 May 2022, in parallel with 113 controls. (cdc.gov)
  • The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. (cancerindex.org)
  • Both V H and V L have the same general structure, with four framework regions (FRs), whose sequences are relatively conserved, connected by three hypervariable or complementarity determining regions (CDRs) (see Kabat et al. (justia.com)
  • Thus the entire SusG sequence can be described from N- to C-terminus (residue numbers): A domain (43-152) - B domain (153-215) - CBM58 (215-335) - B domain (336-363) - A domain (364-607) - C domain (608-692). (cazypedia.org)
  • in most carbohydrates, hydrogen and oxygen are found in the same two-to-one relative proportions they have in water. (chemicalindustrynews.com)
  • Within L. monocytogenes , sequences homologous to gtcA were found in all serogroup 4 isolates but not in strains of any other serotypes. (asm.org)
  • Although the genome of F. virguliforme was sequenced, there were limited studies focused on the PCWDEs of F. virguliforme. (omicsdi.org)
  • These structures may represent progressive reduction of a syncarpous ovary, and the extreme of the reduction sequence, for example in Amyema, is an almost completely undifferentiated ovary with a single group of sporogenous cells at the base of the stylar canal. (cybertaxonomy.org)
  • Finally, although multiple amino acid substitutions of T4 abolished glycosylation at N2 but were not toxic, similar substitutions of T17 were not tolerated, suggesting that the carbohydrate moiety at N15 is critical for cell viability. (jneurosci.org)