• Gene
  • The sex of the sample was determined by PCR amplification of the SRY gene in genomic DNA isolated from the adipose samples. (docplayer.net)
  • human
  • Total RNA was extracted from frozen human adipose samples using TRIzol Reagent according to the protocol provided by the manufacturer (Invitrogen) and QIAGEN RNeasy Mini kit s manual. (docplayer.net)
  • Chromatin immunoprecipitation (ChIP) was performed in frozen adipose samples using the protocol originally designed for human pluripotent stem cells as described previously. (docplayer.net)