• Vivo
  • This mouse uses an inducible, adipose specific LacZ transgene, where one can label all the currently existing adipocytes as LacZ positive (blue stained) and evaluate the new (white, unlabelled) adipocytes in vivo. (wikipedia.org)
  • macrophage
  • Methods and Results- Coculture of differentiated 3T3-L1 adipocytes and macrophage cell line RAW264 results in the marked upregulation of proinflammatory cytokines, such as tumor necrosis factor α (TNF-α), and the downregulation of the antiinflammatory cytokine adiponectin. (ahajournals.org)
  • Marrow
  • MAT, by its "specific marrow location, and its adipocyte origin from at least LepR+ marrow MSC is separated from non-bone fat storage by larger expression of bone transcription factors", and likely indicates a different fat phenotype. (wikipedia.org)
  • Histologic sections demonstrating Marrow Adipocytes Representative distal femur histologic section of a 16-week old healthy C57BL/6 mouse demonstrating a typical quantity of marrow adipocytes. (wikipedia.org)
  • Marrow adipocytes are difficult to isolate and quantify because they are interspersed with bony and hematopoietic elements. (wikipedia.org)
  • proliferation
  • Lipoma in patients with the multiple endocrine neoplasia type 1 (MEN1) syndrome is a type of benign fat-cell tumor that has biallelic inactivation of MEN1 that encodes menin and could serve as a model to investigate normal and pathologic fat-cell (adipocyte) proliferation and function. (hindawi.com)
  • mRNA
  • Hcy (300-1000 μmol/l) also increased mRNA expression of resistin in primary rat adipocytes in a time- and concentration-dependent manner, with maximal induction at 24 h of approximately fourfold with 1,000 μmol/l. (diabetesjournals.org)
  • Adipocytes from females have higher mRNA/protein levels of several genes involved in glucose and lipid metabolism. (diabetesjournals.org)
  • genes
  • The role of menin and its target genes in adipocytes is not known. (hindawi.com)
  • We examined the effects of alliin in lipopolysaccharide- (LPS-) stimulated 3T3-L1 adipocytes by RT-PCR, Western blot, and microarrays analysis of 22,000 genes. (hindawi.com)
  • Incubation of cells for 24 h with 100 μ mol/L alliin prevented the increase in the expression of proinflammatory genes, IL-6, MCP-1, and Egr-1 in 3T3-L1 adipocytes exposed to 100 ng/mL LPS for 1 h. (hindawi.com)
  • knockdown
  • Adipocytes derived from Men1-KO mESCs or after menin knockdown in 3T3-L1 cells showed a 1.5-1.7-fold increase in fat-cell size. (hindawi.com)
  • Human APOE knockdown hMSC-Tert adipocytes accumulated markedly less triglycerides compared to control cells. (osti.gov)
  • cells
  • The aim of this study was to assess both glycerol permeability and metabolism in undifferentiated 3T3-L1 cells (UDCs) as well as in untreated (CTL-DCs) versus lipopolysaccharide (LPS-DCs)-treated differentiated 3T3-L1 adipocytes. (mdpi.com)
  • In the current study, we demonstrate that adipocyte CD1d plays a key role in the stimulation of adipose iNKT cells, leading to anti-inflammatory responses in high-fat diet (HFD)-fed mice. (diabetesjournals.org)
  • Accordingly, adipocyte-specific CD1d-knockout (CD1d ADKO ) mice showed reduced numbers of iNKT cells in adipose tissues and decreased responses to α-galactosylceramide-induced iNKT cell activation. (diabetesjournals.org)
  • Approximately 10% of fat cells are renewed annually at all adult ages and levels of body mass index without a significant increase in the overall number of adipocytes in adulthood. (wikipedia.org)
  • Nevertheless, histological techniques and fixation make possible visualization of MAT, quantification of adipocyte size, and MAT's association with the surrounding endosteum, milieu of cells, and secreted factors. (wikipedia.org)
  • Both adipocytes and brown adipocyte may be derived from pericytes, the cells which surround the blood vessels that run through white fat tissue. (wikipedia.org)
  • knockout
  • Differentiated hMSC-Tert were stably transduced with or without siRNA targeting APOE while murine adipocytes were isolated from wild type and Apoe knockout mice. (osti.gov)
  • Adipocyte-specific Cul4b knockout (AKO) mice exhibited increased adiposity relative to wild-type (WT) control when challenged with a high-fat diet (HFD). (diabetesjournals.org)
  • cell
  • In contrast to the upper body fat cell response, the number of lower-body adipocytes did significantly increase during the course of experiment. (wikipedia.org)
  • This condition cannot be effectively corrected through diet and exercise alone, as the panniculus consists of adipocytes and other supporting cell types shrunken to their minimum volume and diameter. (wikipedia.org)