Addition epitopes. Medical search. Frequent questions

The IEDB contains data related to antibody and T cell epitopes for humans, non-human primates, rodents, and other animal species. (wikipedia.org)
In this work, we isolate a dromedary camel V H H single-domain antibody (also known as nanobody) named D4 and a monoclonal antibody (mAb) named HM2 recognizing membrane-distal and membrane-proximal epitopes of GPC1, respectively. (nature.com)
Epitopes for GAD65 antibody associated with type 1 diabetes are located in the middle region and the COOH-terminal of the GAD65 protein, whereas epitopes associated with SPS reside in the NH2-terminal in addition to the middle region and COOH-terminal. (nel.edu)
We have previously described R1a-B6, an alpaca-derived single domain antibody (nanobody), that is capable of potent cross-subtype neutralization in vitro of H1N1, H5N1, H2N2, and H9N2 influenza viruses, through binding to a highly conserved epitope in the influenza hemagglutinin stem region. (frontiersin.org)
The premise for this vaccine concept rests on (i) the significance of CD4+ T cell memory space to influenza immunity, (ii) the essential role CD4+ T cells perform in development of neutralizing antibodies, (iii) linked specificity of HA-derived CD4+ T cell epitopes to antibody reactions, (iv) the structural plasticity of HA and (v) an illustration of improved antibody response to a prototype manufactured recombinant H7-HA vaccine. (fabretp.org)
The beauty of the antigen - antibody reaction is that each antibody is specific to only a portion of the antigen, called an epitope, and does not bind other molecules that do not match its target, including the body's own molecules. (technologynetworks.com)
Our complete maps of how mutations impact serum antibody binding identify three major epitopes in the RBD. (biorxiv.org)
A better understanding of how antibodies develop membrane affinity and target membrane-proximal epitopes would be impactful for antibody therapeutics, auto-immunity, and vaccine development 10 - 12 . (elifesciences.org)
In addition, ProMIS Neurosciences owns a portfolio of therapeutic and diagnostic patents relating to misfolded SOD1 in ALS, and currently has a preclinical monoclonal antibody therapeutic against this target. (newswire.ca)
In addition, the findings can guide the formulation of effective antibody combinations for therapeutic use. (bvsalud.org)
Our data suggest that antibody epitopes including V2q (e.g. (bvsalud.org)
Sera from each period ( n = 185 and n = 693) were screened in an epitope blocking enzyme immunoassay for flavivirus antibody detection. (who.int)
[ 6 ] First, standard first line therapy for HER2-positive mBC is based on the combination of chemotherapy (namely a taxane) with a dual anti-HER2 blockade with trastuzumab and pertuzumab, a humanized monoclonal antibody targeting another HER2 epitope than trastuzumab. (medscape.com)

In addition, many of the mutations associated with the Omicron variant are found in areas that are likely bound by neutralizing antibodies, suggesting that the first line of immunological defense against COVID-19 may be compromised. (biorxiv.org)
However, both natural infection and vaccination develop T-cell based responses, in addition to antibodies. (biorxiv.org)
These antibodies may be generated against the hormonogenic epitopes of Tg. (avmi.net)
They can bind to epitopes that are not accessible to conventional antibodies because of their small size and shape. (inverse.com)
In addition, they can be easily linked together to create multi-specific antibodies binding to different epitopes on the same or different targets. (inverse.com)
Antibodies are blood proteins that can combine chemically with bacteria and viruses, while epitopes are the part of an antigen molecule where antibodies can attach. (inverse.com)
Binding by polyclonal serum antibodies is affected by mutations in three main epitopes in the RBD, but there is substantial variation in the impact of mutations both among individuals and within the same individual over time. (biorxiv.org)
A potent class of HIV-1 broadly neutralizing antibodies (bnAbs) targets the envelope glycoprotein's membrane proximal exposed region (MPER) through a proposed mechanism where hypervariable loops embed into lipid bilayers and engage headgroup moieties alongside the epitope. (elifesciences.org)
The work provides insights into how broadly neutralizing antibodies associate with lipids proximal to membrane-associated epitopes to drive neutralization. (elifesciences.org)
Antibodies can target epitopes on integral membrane proteins very near to the lipid bilayer surface, even those partially embedded within the headgroup region. (elifesciences.org)
We sought to address this phenomenon for broadly neutralizing antibodies (bnAbs) 4E10, PGZL1, 10E8, and LN01 of unique lineages which all target the semi-concealed membrane-proximal epitope region (MPER) 13 - 16 of the HIV-1 envelope glycoprotein (Env). (elifesciences.org)
In addition, different SARS-CoV-2 epitope antibodies from 135 vaccinated kidney transplant recipients were compared with antibodies in 25 matched healthy controls after second vaccination. (lww.com)
In addition to these autoantibodies, patients with thymoma-associated MG produce autoantibodies to various neuromuscular antigens, including antibodies to the skeletal muscle calcium release channel (ryanodine receptor of sarcoplasmic reticulum) and antibodies to cytoplasmic filamentous proteins (particularly titin) or neurofilaments. (medscape.com)
L104M, L115Q, V210A) between the HA of the virus from the Chilean case and A/Astrakhan/3212/2020-like candidate vaccine, and they are not in major antigenic epitopes strongly suggesting that antibodies elicited by the A/Astrakhan/3212/2020-like vaccine would be expected to have good cross-reactivity - and therefore protection - against this virus. (cdc.gov)

Then, evolving tolerance or even affinity to lipid bilayers could be beneficial in recognition of membrane-proximal epitopes, for example gaining avidity and specificity via cooperative interactions simultaneously with the epitope and membrane. (elifesciences.org)

Scientists analyzed known human CD8+ T cell epitopes from EBV antigens to design a polyepitope vaccine immunogen that incorporates 20 CD8+ T cell epitopes into an engineered protein immunogen. (news-medical.net)
Within this population, only one low-prevalence epitope from the Spike protein restricted to two HLA alleles and found in 2/30 (7%) individuals contained a single amino acid change associated with the Omicron VOC. (biorxiv.org)
In addition to identifying significant binding probes, HERON also provides functions for finding epitopes (string of consecutive peptides within a protein). (bioconductor.org)
HERON also calculates significance on the probe, epitope, and protein level by employing meta p-value methods. (bioconductor.org)
In addition, supplementary protocols for protein purification and other applications are available in the 'Resources' section of this page or at www.preanalytix.com . (qiagen.com)
NetCTL is a web-based tool designed for predicting human CTL epitopes in any given protein. (biomedcentral.com)
According to Coe, Celsee is targeting downstream proteogenomics as one of the first applications for the platform because the market is expanding as researchers not focus on expression data, but also protein epitopes and "we offer the ability to do this at scale. (genomeweb.com)
Although a reliable and reproducible tape-stripping procedure was developed for tripropylene glycol diacrylate (TPGDA) exposed human skin, synthesis of a TPGDA-protein haptenic complex failed to produce epitope specific antisera for quantification. (cdc.gov)
11. Amsel, A. & Roussel, J. Motivational properties of ences in post-transcriptional processes spark interest in the development of frustration: I. Effect on a running response of the (protein redistribution, degradation), pharmacotherapies that selectively reg- addition of frustration to the motivational com- plex. (lu.se)

The inclusion of both latent and lytic antigenic epitopes is intended to induce robust T-cell response targeting different phases of the viral life cycle. (news-medical.net)

Clustered Iridoviridae subfamilies possess host-specific characteristics, which can be considered as exclusive features for in-silico prediction of effective epitopes for vaccine development. (biomedcentral.com)
Host-specific characteristics are important features and constraints for effective epitope prediction. (biomedcentral.com)

This analysis examined if the previously identified viral epitopes targeted by CD8+ T-cells in these individuals (n=52 distinct epitopes) are mutated in the newly described Omicron VOC (n=50 mutations). (biorxiv.org)
In addition, 38 constructs were also created through a collaboration with Harvard university, aiming at mimicking the structure of the MPER on the surface of the viral particles. (europa.eu)
Importantly, CD4+ and CD8+ T-cell clones specific for viral epitopes from one strain often showed diminished recognition of the corresponding epitopes in other strains. (nih.gov)
These results demonstrate that viral strain heterogeneity may impair antiviral T-cell immunity and suggest that immunotherapeutic approaches against EBV should preferably target broad sets of conserved epitopes including their flanking regions. (nih.gov)

Collectively, ROS caused extensive damage to Hb altering its immunogenicity, the neo-epitopes, thus generated might play a role in the induction of circulating autoantibodies in type 1 diabetes mellitus. (scialert.net)
Among gluten proteins, α-gliadins display the strongest immunogenicity with four main T-cell stimulatory epitopes. (biomedcentral.com)
Spring accessions showed a significantly higher immunogenicity than winter ones and no influence of spelt breeding on the epitope expression levels could be assessed when comparing landraces and varieties from Northwestern Europe. (biomedcentral.com)

The peptide epitopes were restricted through several common HLA class I alleles for broader coverage across many ethnic groups. (news-medical.net)
This successful demonstration of an antigenic epitope using this method provides a model for synthesis of an acrylated cysteine for synthesis of an antigenic keratin-lor keratin-lO peptide for preparation of an immunogenic complex. (cdc.gov)

In addition, T-cell recognition of a conserved epitope was affected by amino acid exchanges within the epitope flanking region. (nih.gov)

These rare mAbs bind to functionally conserved epitopes such as those in the hemagglutinin (HA) stem, thereby providing strain independent protection. (frontiersin.org)

In addition to the database, the IEDB website hosts an Analysis Resource, which contains a collection of tools to predict and analyze epitopes. (wikipedia.org)
The objective of this work was to study the expression of α-gliadin epitopes related to CD in a wide collection of 121 spelt accessions (landraces and varieties, spring and winter accessions) from different provenances, and to analyze the correlation between the presence of epitope sequences in gDNA and their expression (cDNA). (biomedcentral.com)

We conduct a comprehensive set of experiments using the latest Immune Epitope Database (IEDB) datasets. (nec-labs.com)
We use two evaluation datasets consisting of known HIV CTL epitopes and their source proteins. (biomedcentral.com)

HA incorporates the major epitopes conferring protective immunity ( 1 - 3 ). (cdc.gov)
However, many cross-reactive conserved epitopes are often occluded on virus particles, contributing to the evasion of humoral immunity. (bvsalud.org)
Given that, bNAbs are ideal for providing sterilizing immunity and are the goal of current HIV-1 vaccine efforts, these data offer insight on how HIV-1 may occlude these vulnerable epitopes from the host immune response. (bvsalud.org)

The Immune Epitope Database and Analysis Resource (IEDB) is a project hosted by scientists at the La Jolla Institute for Allergy and Immunology (LIAI), with support from the National Institute of Allergy and Infectious Diseases (NIAID), a part of the National Institutes of Health[permanent dead link] (NIH), and Department of Health and Human Services (HHS). (wikipedia.org)

Among them, 42 could be produced and characterized for solubility, oligomerization state, thermal stability, epitope exposure and structural properties. (europa.eu)
Cross-reactivity risk analysis of the candidates TCRs showed high binding stability to MAGE-A3-Mp4 epitope and low risk of cross-reaction. (thno.org)
A relative stability of the amount of epitopes expressed by a same accession across four harvest years was detected. (biomedcentral.com)

Only one of 52 epitopes identified in this population contained an amino acid that was mutated in Omicron. (biorxiv.org)

Reliable predictions of Cytotoxic T lymphocyte (CTL) epitopes are essential for rational vaccine design. (biomedcentral.com)
Reliable predictions of immunogenic peptides can minimize the experimental effort needed to identify new epitopes to be used in, for example, vaccine design or for diagnostic purposes. (biomedcentral.com)

The testing of the polyepitope vaccine immunogen using peripheral mononuclear cells isolated from EBV seropositive donors revealed that the immunogen can be effectively processed by antigen-presenting cells to generate epitopes that can be presented on HLA class I molecules and subsequently recognized by human CD8+ T cells to promote an immune response. (news-medical.net)

We identified the HLA-A2 restricted T cell epitopes from MAGE-A3 that could effectively induce the activation and cytotoxicity of CD8+ T cells using artificial APC in vitro . (thno.org)

In addition, epitopes from other infectious agents, allergens and autoantigens are being curated. (wikipedia.org)

Statistical and machine-learning based analyses demonstrated that the MAGE-A3-Mp4 epitope-specific CD8+ T cell clones were mostly in effector and proliferating state. (thno.org)

A cohort of HLA-A2+ NSCLC donors demonstrated that the number of epitope specific CD8+ T cells increased in NSCLC than healthy controls when measured with tetramer derived from the candidate MAGE-A3 epitopes, especially epitope Mp4 (MAGE-A3: 160-169, LVFGIELMEV). (thno.org)

This study examined if the parts of the virus, or epitopes, targeted by the CD8+ T-cell response in thirty individuals who recovered from COVID-19 in 2020 were mutated in the Omicron variant. (biorxiv.org)
However, limited data are available about sequence variability in CD8 + T cell epitope regions of mumps virus (MuV) proteins. (mdpi.com)
In the present study, sequences of 40 CD8 + T cell epitope candidates, including previously and newly identified, obtained from Jeryl-Lynn mumps vaccine strains were compared with genomes from 462 circulating MuV strains. (mdpi.com)
In this study, we comprehensively screened HLA-A2 restricted MAGE-A3 tumor epitopes and characterized the corresponding TCRs using in vitro artificial antigen presentation cells (APC) system, single-cell transcriptome and TCR V(D)J sequencing, and machine-learning. (thno.org)
However, when T-cell epitopes including their flanking regions were compared, a substantial proportion was found to be polymorphic in different EBV strains. (nih.gov)
In addition, the early users commented on Genesis' ability to dramatically reduce the per-cell analysis cost, batch effects, and running time, as well its high throughput due to automated processing. (genomeweb.com)

The Company applies its thermodynamic, computational discovery platform-ProMIS ™ and Collective Coordinates - to predict novel targets known as Disease Specific Epitopes (DSEs) on the molecular surface of misfolded proteins. (newswire.ca)

The experimental results showed that four conserved epitopes among the Iridovirideae family, one exclusive epitope for invertebrate subfamily and two exclusive epitopes for vertebrate family were predicted. (biomedcentral.com)
Most importantly, they can minimize the experimental effort needed to identify epitopes. (biomedcentral.com)

In addition, we observed Tau-C3 immunoreactivity in extracellular tangles, suggesting that the Tau-C3 epitope is characteristically stable. (springer.com)

Scientists from Australia and the USA have developed a lymph node-targeted multi-epitope subunit vaccine that induces strong humoral and cellular immune responses against Epstein-Barr virus (EBV) in mice. (news-medical.net)

Several other groups have likewise attempted to generate methods that enable CTL epitope identification. (biomedcentral.com)

TaqMan probes targeting the canonical form of the epitopes were used to evaluate the epitope expression levels. (biomedcentral.com)

There are differences in recognized epitopes among the three disorders. (nel.edu)
The present case suggests that these differences in epitopes may be related to various pathogenic mechanisms including glucose intolerance. (nel.edu)

These epitopes become accessible after allosteric conformational changes are induced by the pre-binding of select Abs, prompting us to test if similar conformational changes are required for these Abs to exhibit their neutralization capability. (bvsalud.org)
These data provide insight on how bNAbs may gain access to these occluded epitopes to exert their neutralization effects and block HIV-1 infection. (bvsalud.org)

barley ( Hordeum vulgare L.) and rye ( Secale cereale L.). Oat ( Avena sativa L.) also displays a small fraction of prolamin storage proteins, called avenins, which do not contain any CD-related epitopes [ 3 ]. (biomedcentral.com)

No correlation was observed between quantitative PCR results obtained from cDNA and gDNA for 45 accessions tested, stressing the need to use markers focusing on epitope transcripts rather than on genomic sequences. (biomedcentral.com)

Fifty-one vaccine candidates were designed mainly to mimic fusion intermediate conformations of gp41 and in some instance to increase exposure of the 2F5/4E10 epitopes. (europa.eu)

We further show that lectin-glycan interactions can also expose these epitopes. (bvsalud.org)

In addition, the text would benefit from clearer subsections that delineate discrete mechanistic discoveries. (elifesciences.org)
In addition, recent discoveries have challenged the current epidemiological model of EVD in which the virus is introduced in a community of naïve individuals via zoonotic spillover. (who.int)

Significant variations in the amount of epitope transcripts were identified between accessions and according to the provenances. (biomedcentral.com)

In addition, NHANES provides the means to better define the epidemiology of other hepatitis viruses. (cdc.gov)

It has general information, but you can find some good information here in addition to the variants page, which Dr. Barnes wil present on a little bit later. (cdc.gov)

The focus is dissemination of immune epitope information to facilitate the generation of new research tools, diagnostic techniques, vaccines and therapeutics. (wikipedia.org)

In addition, we develop a new position encoding method in ConvM and SpConvM to better identify the most important amino acids for the binding events. (nec-labs.com)
This study aimed to identify the Env epitopes that are exposed/occluded on HIV-1 particles and to investigate the mechanisms contributing to their masking. (bvsalud.org)

Another design strategy was developed using gammaretroviruses backbone where 2 domains were substituted with HIV epitopes in the N-terminal part of the ectodomain and the membrane proximal external region (MPER) respectively. (europa.eu)

In this study, scientists have developed a lymph node-targeting multi-epitope subunit vaccine against EVB and tested its efficacy in Human Leukocyte Antigen (HLA)-expressing mice. (news-medical.net)
Recently, the first set of naturally presented human leukocyte antigen Class I (HLA-I) epitopes of MuV was identified by us. (mdpi.com)
In addition, a reliable and reproducible tape-stripping procedure for sampling and isolation of naphthalene-keratin adducts (antigen) from the stratum corneum under defined laboratory conditions was developed and tested on human volunteers exposed to jet fuel instead of TPGDA. (cdc.gov)

This study demonstrates the potential of V H H-based CAR T for pancreatic cancer therapy and provides an engineering strategy for developing potent CAR T cells targeting membrane-distal epitopes. (nature.com)

Any anti-HAV in the test sample competes with the conjugate for HAV epitopes present on the bead. (cdc.gov)

Results indicate that a fraction of Tg AA recognizes T3-containing epitopes in Tg. (avmi.net)

The effect of environmental factors (harvest year and N fertilization) on the epitope expression was also investigated. (biomedcentral.com)

In addition to cleaving effective when administered within 24 hr of exposure [1]. (cdc.gov)

Anatomy17

Organisms13

Diseases3

Chemicals and Drugs96

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes36

Disciplines and Occupations1

Information Science4

Health Care2

Antibody13

Antibodies14

Membrane-proximal epitopes1

Protein9

Antigenic epitopes1

Prediction2

Viral4

Immunogenicity3

Peptide2

Recognition1

Bind1

Analyze2

Datasets2

Immunity3

IEDB1

Stability3

Amino acid1

Vaccine design2

Immune response1

Induce1

Infectious1

Clones1

Donors1

Cell6

Molecular1

Experimental2

Extracellular1

Humoral1

Generate1

Evaluate1

Differences2

Neutralization2

Proteins1

Sequences1

Candidates1

Interactions1

Discoveries2

Significant1

Viruses1

Variants1

Vaccines1

Identify2

Region1

Human3

Study1

Present1

Results1

Effect1

Effective1