• MAPK
  • The Drosophila gene mushroom bodies tiny ( mbt ) encodes a putative p21-activated kinase (PAK), a family of proteins that has been implicated in a multitude of cellular processes including regulation of the cytoskeleton, cell polarisation, control of MAPK signalling cascades and apoptosis. (biologists.org)
  • mammalian cells
  • Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells Mo Zhou 1 , Mark R. Philips 1 1 Langone Medical Center, New York University Here we present protocols for detergent-free homogenization of cultured mammalian cells based on nitrogen cavitation and subsequent separation of cytosolic and membrane-bound proteins by ultracentrifugation. (jove.com)
  • The internalization of plasma membrane proteins by mammalian cells is crucial for many essential cellular processes, such as nutrient uptake, control of ion channels, retrieval of synaptic vesicle components in neurons, and the regulated expression of signaling receptors and adhesion molecules at the cell surface ( Conner and Schmid, 2003 ). (biologists.org)
  • Drosophila
  • In a screen for viable mutations affecting adult central brain structures, the mushroom bodies tiny ( mbt ) gene of Drosophila, which encodes a protein related to p21-activated kinase (PAK), has been isolated. (sdbonline.org)
  • homology
  • The Eps15-homology (EH) domain is a highly conserved motif comprising ∼100 residues that is found in proteins from species as diverse as yeast and mammals. (biologists.org)
  • 1995
  • The β and γ subunits of the G‐protein then activate a MAP kinase cascade consisting of Ste7p (a MAP kinase kinase or MEK homolog), Ste11p (a MEK kinase homolog) and the partially redundant MAP kinase homologs Fus3p and Kss1p ( Herskowitz, 1995 ). (embopress.org)
  • localization
  • Subcellular localization of wild‐type and mutant Ste20p fused to green fluorescent protein showed that the Cdc42p binding domain is needed to direct localization of Ste20p to regions of polarized growth. (embopress.org)
  • carboxyl-terminal
  • Direct visualization of the gamma secretase-generated carboxyl-terminal domain of the amyloid precursor protein: association with Fe65 and translocation to the nucleus. (virginia.edu)
  • plasma membrane
  • Following growth factor stimulation, binding of SH2 domains of Grb2 to the autophosphorylated receptor localizes Sos adjacent to Ras in the plasma membrane, thereby enabling Sos to activate Ras. (embopress.org)
  • Just as important is the ability to recycle a subset of internalized proteins to the plasma membrane to partake in additional rounds of internalization. (biologists.org)
  • 2002
  • Different PAK proteins can also exert pro- and anti-apoptotic effects (reviewed by Jaffer and Chernoff, 2002 ). (biologists.org)
  • regulates
  • In summary, our studies reveal a novel G protein-coupled receptor senses nutrients and regulates the dimorphic transition to filamentous growth via a Gα protein-cAMP-PKA signal transduction cascade. (genetics.org)
  • In Saccharomyces cerevisiae , a single cyclin-dependent kinase, Cdc28, regulates both G1/S and G2/M phase transitions by associating with stage-specific cyclins. (sdbonline.org)
  • motifs
  • Each EH domain contains two calcium-binding helix-loop-helix motifs known as EF-hands, which are linked by a short anti-parallel β-sheet. (biologists.org)
  • sevenless
  • The Son of Sevenless (Sos) proteins control receptor-mediated activation of Ras by catalyzing the exchange of guanosine diphosphate for guanosine triphosphate on Ras. (embl-heidelberg.de)
  • distinct
  • Localisation of PAK proteins to distinct cellular compartments appears to be an important mechanism to control their function in the reorganisation of the cytoskeleton. (biologists.org)
  • domain
  • While different subfamilies of Ras superfamily GTPases have a conserved GTP binding domain, this is not the case for GEFs. (wikipedia.org)
  • WASp and N-WASP are analogs, they contain an N-terminal EVH1 domain, a C-terminal VCA domain and central B and GBD (GTP binding domain) domains. (wikipedia.org)
  • Taxonomic distribution of proteins containing RasGEF domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with RasGEF domain is also avaliable . (embl-heidelberg.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing RasGEF domain in the selected taxonomic class. (embl-heidelberg.de)
  • Here we report that the synprint site can also directly bind the μ subunit of AP-2, an adaptor protein for clathrin-mediated endocytosis, in competition with the synaptotagmin 1 (Syt 1) C2B domain. (jneurosci.org)
  • C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH? (biologists.org)
  • Indeed, C-terminal EH-domain-containing proteins appear to regulate several key endocytic steps, including internalization and recycling. (biologists.org)
  • Moreover, the Cdc42p binding domain was required for cell-cell adhesion during conjugation. (embopress.org)
  • differentiation
  • The mutant phenotype of mbt is characterised by fewer neurones in the brain and the eye, indicating a role of the protein in cell proliferation, differentiation or survival. (biologists.org)