• homology
  • In contrast, WAVE subfamily proteins are characterized by the presence of the WHD/SHD domain (WAVE homology domain/SCAR homology domain), which is located at the amino terminus. (biomedcentral.com)
  • Knudsen BS, Feller SM, Hanafusa H. Four proline-rich sequences of the guanine-nucleotide exchange factor C3G bind with unique specificity to the first Src homology 3 domain of Crk. (springer.com)
  • The Eps15-homology (EH) domain is a highly conserved motif comprising ∼100 residues that is found in proteins from species as diverse as yeast and mammals. (biologists.org)
  • mammalian
  • Many of the mammalian Dbl family proteins are tissue-specific and their number in Metazoa varies in proportion of cell signaling complexity. (wikipedia.org)
  • Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells Mo Zhou 1 , Mark R. Philips 1 1 Langone Medical Center, New York University Here we present protocols for detergent-free homogenization of cultured mammalian cells based on nitrogen cavitation and subsequent separation of cytosolic and membrane-bound proteins by ultracentrifugation. (jove.com)
  • Ypk1 is a central regulator of pathways and processes required for plasma membrane lipid and protein homeostasis, and requires phosphorylation on its T-loop by eisosome-associated protein kinase Pkh1 (mammalian ortholog is PDK1) and a paralog (Pkh2). (mdpi.com)
  • The internalization of plasma membrane proteins by mammalian cells is crucial for many essential cellular processes, such as nutrient uptake, control of ion channels, retrieval of synaptic vesicle components in neurons, and the regulated expression of signaling receptors and adhesion molecules at the cell surface ( Conner and Schmid, 2003 ). (biologists.org)
  • scaffold
  • Other functions of WASP depend on its activity as a scaffold protein for assembly of effective signalling complexes downstream of antigen receptor or integrin engagement. (wikipedia.org)
  • In addition to its role in vesicle trafficking and tethering, Cdc42 GTP might stimulate the assembly of a protein scaffold that organizes the sequence of the individual steps of exocytosis at the cortex of the bud ( Liu and Novick, 2014 ). (biologists.org)
  • vesicle
  • The essential PH domains of Boi1p and Boi2p interact with Sec1p, a protein required for SNARE complex formation and vesicle fusion. (biologists.org)
  • signal transduc
  • Studies over the past several years have elucidated the mechanism whereby SH2 domain‐containing proteins without catalytic activity, such as Grb2‐ and p85‐associated PI3 kinase, regulate signal transduction pathways. (embopress.org)
  • This report identifies a novel function for a PAK-homologous protein and suggests a potential unique mechanism by which other signal transduction pathways may cross-talk with AR pathways to regulate AR function in normal and malignant prostate cells (Yang, 2001). (sdbonline.org)
  • regulate the activity
  • In particular, as discussed here, septin-based structures recruit, and thereby localize (and, in some cases, regulate the activity of) a multiplicity of protein kinases that integrate multiple inputs into signaling pathways and ultimately initiate ensuing biological responses (Figure 1 ). (frontiersin.org)
  • GTPases
  • GTPases contain two loops called switch 1 and switch 2 that are situated on either side of the bound nucleotide. (wikipedia.org)
  • motifs
  • The presence of a number of different motifs suggests they are regulated by a number of different stimuli, and interact with multiple proteins. (wikipedia.org)
  • Each EH domain contains two calcium-binding helix-loop-helix motifs known as EF-hands, which are linked by a short anti-parallel β-sheet. (biologists.org)
  • sevenless
  • The Son of Sevenless (Sos) proteins control receptor-mediated activation of Ras by catalyzing the exchange of guanosine diphosphate for guanosine triphosphate on Ras. (embl-heidelberg.de)
  • cascade
  • The RhoGTPase Cdc42p, the master regulator of polar growth in yeast and other eukaryotes, is at the top of the cascade of proteins that initiates and maintains the polarity of the cell ( Bi and Park, 2012 ). (biologists.org)
  • In summary, our studies reveal a novel G protein-coupled receptor senses nutrients and regulates the dimorphic transition to filamentous growth via a Gα protein-cAMP-PKA signal transduction cascade. (genetics.org)
  • domain
  • WASp and N-WASP are analogs, they contain an N-terminal EVH1 domain, a C-terminal VCA domain and central B and GBD (GTP binding domain) domains. (wikipedia.org)
  • Taxonomic distribution of proteins containing RasGEF domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with RasGEF domain is also avaliable . (embl-heidelberg.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing RasGEF domain in the selected taxonomic class. (embl-heidelberg.de)
  • Here we report that the synprint site can also directly bind the μ subunit of AP-2, an adaptor protein for clathrin-mediated endocytosis, in competition with the synaptotagmin 1 (Syt 1) C2B domain. (jneurosci.org)
  • C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH? (biologists.org)
  • Indeed, C-terminal EH-domain-containing proteins appear to regulate several key endocytic steps, including internalization and recycling. (biologists.org)
  • cells
  • The Wiskott-Aldrich Syndrome protein (WASp) is a 502-amino acid protein expressed in cells of the hematopoietic system. (wikipedia.org)
  • The WASp gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. (wikipedia.org)
  • Here, we show that the proteins Boi1p and Boi2p are important elements of this area of active exocytosis at the tip of growing yeast cells. (biologists.org)
  • 2002
  • Different PAK proteins can also exert pro- and anti-apoptotic effects (reviewed by Jaffer and Chernoff, 2002 ). (biologists.org)
  • substrates
  • This review summarizes what we currently understand about how the action of septin-associated protein kinases and their substrates control information flow to drive the cell cycle into and out of mitosis, to regulate bud growth, and especially to direct timely and efficient execution of cytokinesis and cell abscission. (frontiersin.org)
  • gene
  • The expression of the recently identified dermokine (Dmkn) gene leads to four families of proteins with as yet unknown functions. (jove.com)