• Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
  • The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
  • These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)
  • Apoptosis is an important mechanism in regulating K562 cells and can be induced by the changes in the metabolic state of the cells. (wikipedia.org)
  • There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
  • This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
  • When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
  • Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • The result on this occasion was the initiation of apoptosis (programmed cell death) in cancer cells, which shows that curcumin can be an anti-cancer agent. (antiaging-systems.com)
  • Numerous studies currently suggest that quadruplex formation in this overhang alters the structure and function of telomeres, inducing a damage response and rapid apoptosis in particular in cancer cells [ 14 - 24 ]. (hindawi.com)
  • AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)
  • It is thus concluded that AVM inhibits the activity of BCR/ABL and their subsequent molecular signals, including AKT and MAPK, resulting in cytotoxicity via apoptosis. (spandidos-publications.com)
  • Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • It led to loss of Δψm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. (edu.hk)
  • The aim of this study was designed to investigate whetherRg1 could induce apoptosis in TF-1/Epo cells and further to explore the underlying molecular mechanisms. (waocp.org)
  • Herewe found that Rg1 could inhibit TF-1/Epo cell proliferation and induce cell apoptosis in vitro in a concentrationand time dependent manner. (waocp.org)
  • 2014). 'Ginsenoside Rg1 Induces Apoptosis through Inhibition of the EpoR-Mediated JAK2/STAT5 Signalling Pathway in the TF-1/Epo Human Leukemia Cell Line', Asian Pacific Journal of Cancer Prevention , 15(6), pp. 2453-2459. (waocp.org)
  • Our NK-Activity ePBMC® have been characterized for cytolytic activity against K562 tumor cells. (immunospot.com)
  • However, solid tumor cells at rest are known to secrete either very low amounts of galectin-9 or, in most of the cases, do not secrete it at all. (bmj.com)
  • It's been proven that long-term publicity of tumor cells for some varieties of chemotherapy medications causes level of resistance [21]. (cancerrealitycheck.com)
  • Other T lymphocytes (ie, T-suppressor/cytotoxic cells) possess the killing function and also inhibit B-cell development. (medscape.com)
  • Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
  • Methods A wide range of human cancer cell lines derived from solid tumours, keratinocytes and primary embryonic cells were employed, together with helper and cytotoxic T cell lines and human as well as mouse primary T cells. (bmj.com)
  • These NK cells demonstrate significant cytotoxic activity against human AML and ALL cell lines and patient leukemia blasts. (biologynews.net)
  • The nitrogen mustards and folate and purine analogs were much later shown to interfere with DNA replication, in part explaining their anti-tumor activity. (biomedcentral.com)
  • Our previous study demonstrated thatRg1 had anti-tumor activity in K562 leukemia cells. (waocp.org)
  • Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active regulatory region sequencing (ExP STARR-seq) and applied it to examine the combinatorial compatibilities of 1,000 enhancer and 1,000 promoter sequences in human K562 cells. (nature.com)
  • High-throughput GLP-capable target cell visualization assay for measuring cell-mediated cytotoxicity. (immunospot.com)
  • Potentiation of vincristine-induced growth inhibition by these MFs was also tested in K562/ADM cells by means of WST-1 [2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay. (elsevierpure.com)
  • MTT assay was used to evaluate cell growth inhibition. (edu.hk)
  • Here, by exploiting a high-throughput enhancer reporter assay, we unravel a set of mammalian promoters displaying enhancer activity. (nih.gov)
  • The relative contents of interleukin- (IL-) 1ß, tumor necrosis factor- (TNF-) α, IL-6, and the chemokine (C-C motif) ligand 2 (CCL2) in the colon tissues and HHT-treated RAW264.7 cells were detected with the enzyme-linked immunosorbent assay. (bvsalud.org)
  • Here, we use a human cell-based reporter assay to characterize off- target cleavage of Cas9-based RGENs. (cdc.gov)
  • Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line. (shen-nong.com)
  • K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
  • K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. (wikipedia.org)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • When given to mice with aggressive human leukemias, these NK cells reduced the circulating human acute lymphocytic leukemia (ALL) and acute myelogenous leukemia (AML) cells by 60 to 85 percent. (biologynews.net)
  • He sought to determine how different levels of these antioxidants may affect oxidative activation of VP-16 in K562 human leukemia cells and the extent of cytotoxicity of VP-16 in these cells. (aicr.org)
  • The expanded NK (D2NK) cell has strong natural killing activity against both K562 and Vero cells, and killed the WNV infected Vero cells through antibody-dependent cellular cytotoxicity (ADCC). (fda.gov)
  • Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. (edu.hk)
  • Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
  • Evidence for distinction of the differentiation-inducing activities and cytostatic properties of 9-(2-phosphonylmethoxyethyl)adenine and a variety of differentiation-inducing agents in human erythroleukemia K562 cells. (aspetjournals.org)
  • The acyclic nucleoside phosphonate derivative 9-(2-phosphonylmethoxyethyl) adenine (PMEA) is a strong inducer of differentiation in several tumor cell lines, including human erythroleukemia K562 cells. (aspetjournals.org)
  • This cell line proved to be insensitive to the induction of erythroid differentiation by PMEA. (aspetjournals.org)
  • The K562/PMEA-1 cells showed reduced sensitivity to the differentiation-inducing capacity and cytostatic activity of several closely related PMEA analogs. (aspetjournals.org)
  • Furthermore, the mutant cells exhibited a decreased sensitivity to the differentiation-inducing activity of a wide variety of structurally nonrelated antimetabolites targeted at different enzymes of nucleotide biosynthesis. (aspetjournals.org)
  • A 5-25-fold higher concentration of each of these compounds was required to obtain the same level of differentiation in the K562/PMEA-1 cells as in the wild-type cells. (aspetjournals.org)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
  • We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
  • Our data clearly suggest that granulocytic AML differentiation relies on noncanonical autophagy pathways and that restoring autophagic activity might be beneficial in differentiation therapies. (hindawi.com)
  • In addition, HHT inhibited the M1 polarization of both human and mouse macrophages, while HHT did not affect the differentiation of human CD4 T cells into Th17, Th1, or Treg cells and did not affect the proliferation and migration of human colon epithelial cells. (bvsalud.org)
  • The Leukemia-Lymphoma Cell Line Factsbook. (wikipedia.org)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
  • In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
  • 1 Wnt/b-catenin is also known to drive the emergence and maintenance of leukemia stem cells in AML. (haematologica.org)
  • A) Immunoblot of myeloid leukemia cell lines showing the relative level of b-catenin (~92 kDa) and WT1 (~50 kDa) protein, with b-actin (42 kDa) used to assess protein loading. (haematologica.org)
  • Interestingly, JOA possessed stronger anti-leukemia activity than its analogues such as OGP46 and Oridonin, which has been investigated extensively. (jcancer.org)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. (edu.hk)
  • on human chronic myeloid leukemia K562 cells. (edu.hk)
  • Chronic myeloid neoplasms are malignant clonal hematopoietic stem cell disorders driven by recurrent genetic events, with an inherent risk of transformation to acute myeloid leukemia (AML) 1 , 2 . (nature.com)
  • Ganoderma Lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function. (shen-nong.com)
  • Researchers from The University of Texas M. D. Anderson Cancer Center have found a therapy that effectively kills human leukemia cells in mice using natural killer (NK) cells from umbilical cord blood. (biologynews.net)
  • Patrick Zweidler-McKay, M.D., Ph.D., assistant professor of pediatrics from the Children's Cancer Hospital at M. D. Anderson, has shown an effective method for expanding the number of NK cells from a single cord blood unit while retaining the cells' anti-leukemia effects, as presented at the American Society of Pediatric Hematology/Oncology annual conference on May 16. (biologynews.net)
  • However, Zweidler-McKay and co-senior investigator Elizabeth Shpall, M.D., professor in M. D. Anderson's Department of Stem Cell Transplantation and Cellular Therapy, have found a novel process to increase NK cells in cord blood more than 30-fold, generating more than 150 million NK cells from one cord blood unit while maintaining their activation to find and kill acute leukemia cells. (biologynews.net)
  • CRISPR/Cas9 Genome Editing of the Human Topoisomerase II Intron 19 5' Splice Site Circumvents Etoposide Resistance in Human Leukemia K562 Cells. (osu.edu)
  • hsa-miR-9-3p and hsa-miR-9-5p as Post-Transcriptional Modulators of DNA Topoisomerase II in Human Leukemia K562 Cells with Acquired Resistance to Etoposide. (osu.edu)
  • Around the same time, and building on the observation that the vitamin folic acid could stimulate acute lymphoblastic leukemia (ALL) cells, Farber used folate analogs such as aminopterin and then amethopterin (methotrexate) to treat ALL, in what is often heralded as the first 'rational' drug development approach [ 4 ]. (biomedcentral.com)
  • 50% of all common SNPs in a hepatocarcinoma and a myeloid leukemia cell line. (biologists.com)
  • Enhancer activity map of a genomic region snapshot containing the Chronic Myeloid Leukemia (CML)-associated variant rs4869742 in K562 cells. (biologists.com)
  • The hematopoietic tumor suppressor interferon regulatory factor 8 (IRF8) is upregulated by the antimetabolite cytarabine in leukemic cells involving the zinc finger protein ZNF224, acting as a cofactor of the Wilms' tumor gene 1 (WT1) protein. (lu.se)
  • Treatment of leukemic K562 cells with cytarabine upregulated IRF8 protein and mRNA, which was correlated to increased expression of ZNF224. (lu.se)
  • We conclude that cytarabine-induced upregulation of the IRF8 in leukemic cells involves increased levels of ZNF224, which can counteract the repressive activity of WT1 on the IRF8-promoter. (lu.se)
  • The impact of miR-146b-5p and TRAF6 on proliferation and migration of CRC cells was determined using Cell Counting Kit-8 and Transwell assays, respectively. (bvsalud.org)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • Further, C.decandratreatment altered the mitochondrial membrane potential in AGS cells. (pdfplayer.org)
  • The objective was to define the mechanism of the growth inhibition of active hexose correlated compound (AHCC) alone and evaluate its activity in combination with pegylated liposomal doxorubicin (PLD). (researchgate.net)
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
  • The cAMP-dependent pathway was activated in K562 cells using the adenylate cyclase activator forskolin. (elsevierpure.com)
  • The cAMP-dependent pathway was found to be activated in K562 cells in which the cGMP-dependent pathway was activated by hemin. (elsevierpure.com)
  • Activation of the cAMP-dependent pathway by forskolin inhibited hemin-induced expression of γ-globin mRNA and decreased transcriptional activity of the γ-globin gene promoter. (elsevierpure.com)
  • These results suggested that the cAMP-dependent pathway, which is independent of MAPK pathways, plays a negative role in γ-globin gene expression in K562 cells. (elsevierpure.com)
  • Recent studies revealed that NOP2/Sun RNA methyltransferase 5 (NSUN5) - ferritin heavy chain (FTH1) pathway is associated with ferroptosis in stem cells, whereas its roles in gastric cancer are still unclear. (bvsalud.org)
  • It also suppressed the expression of EpoR on the surface membrane and inhibitedJAK2/STAT5 pathway activity. (waocp.org)
  • We observed a statistically significant correlation between b-catenin and WT1 expression across 16 myeloid cell lines, with 50% (8/16) co-expressing b-catenin and WT1 to varying degrees ( Figure 1A , B). There was no particular association of this correlation with cell line morphology or genotype. (haematologica.org)
  • CONCLUSIONS: Co-culture of PBMC with K562D2 stimulatory cells is an efficient technique to prepare large quantities of pure and active NK cells, and these expanded NK cells inhibited WNV infection of Vero cells through both cytolytic and noncytolytic activities, which may imply a potential role of NK cells in combating WNV infection. (fda.gov)
  • CapStarr-seq analysis of DnaseI regions in K562 and HeLa cell lines (2 replicates) and in the plasmid library before transfection (Input). (nih.gov)
  • These dihydro-benzoxepins have shown antitumor activity against human tumor cell lines HeLa and K562. (scentopia-singapore.com)
  • The IC50 of phenytoin sodium and doxorubicin in K562/Dox cells was considerably greater than that in wild-type K562 cells indicating the medication level of resistance of K562/Dox cells. (cancerrealitycheck.com)
  • Through the preventing of p38 activity in the current presence of SB202190 cellular number was considerably reduced following the phenytoin sodium and doxorubicin treatment as well as the IC50 of phenytoin sodium and doxorubicin was Isorhynchophylline reduced in K562/Dox cells. (cancerrealitycheck.com)
  • After preventing p38 with 10 μM SB202190 in K562/Dox cells we noticed the fact that IC50 of phenytoin was considerably reduced from 2186.33±214.70 to 949.83±131.31 μg/mL with an RI of 2.30 much like that of the verapamil control (2.56). (cancerrealitycheck.com)
  • The IC50 of doxorubicin was also low in cells treated with 10 μM SB202190 than in neglected K562/Dox cells (4.33±0.50 μg/mL and 0.40±0.09 μg/mL respectively) with an RI of 10.83 much like that of verapamil (12.37). (cancerrealitycheck.com)
  • The focus … Desk 1 IC50 of doxorubicin and phenytoin in K562 and Isorhynchophylline K562/Dox cells. (cancerrealitycheck.com)
  • K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
  • Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
  • A PMEA-resistant K562 cell line (designated K562/PMEA-1) was selected in the presence of escalating PMEA concentrations. (aspetjournals.org)
  • SP1 and GATA3) and HDAC2-GATA1 regulatory networks on rescued regions in K562 cell line. (biomedcentral.com)
  • Additionally, we observed increased apoptotic morphological changes in berberine treated group.Mangrove fraction exhibit potent antiproliferative effect on AGS cell line and upon further will going to check invivo experimental animal studies. (pdfplayer.org)
  • Shallangwa, G. Insilico Modelling of Quantitative Structure-Activity Relationship of Pgi50 Anticancer Compounds on k-562 Cell Line. (ufv.br)
  • The results demonstrated that miR-146b-5p was highly expressed in CRC tissue samples compared with in normal adjacent tissue samples and in CRC cells compared with in the normal NCM460 cell line, whereas TRAF6 was expressed at low levels. (bvsalud.org)
  • Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
  • alone and evaluate its activity in combinatio n with pegylated liposomal doxorubicin (PLD). (researchgate.net)
  • HPLC demonstrated the fact that intracellular degrees of phenytoin sodium and doxorubicin had been considerably low in K562/Dox cells than those in K562 cells. (cancerrealitycheck.com)
  • The intracellular degrees of phenytoin and doxorubicin had been considerably low in K562/Dox cells than those in K562 cells (Body 4A B) additional confirming the medication level of resistance of K562/Dox cells. (cancerrealitycheck.com)
  • The loss of the intracellular degree of phenytoin and doxorubicin in K562/Dox cells was considerably abolished in the current presence of SB202190 (Body 4). (cancerrealitycheck.com)
  • K562/Dox cells had been pretreated with SB202190 for one hour and treated with phenytoin (A) or doxorubicin (B) for 48 hours in the current presence of SB202190. (cancerrealitycheck.com)
  • Verapamil was utilized Isorhynchophylline as a confident … Body 4 Aftereffect of SB202190 Isorhynchophylline on focus of doxorubicin and phenytoin in K562/Dox cells. (cancerrealitycheck.com)
  • In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
  • CCK-8 kits were used to determine cell viability. (bvsalud.org)
  • NSUN5 inhibited ferroptosis including decreasing cell viability and increasing levels of lipid ROS and Fe2+ in GC cells. (bvsalud.org)
  • Raw and processed data for ExP STARR-seq, motif ExP STARR-seq, HS-STARR-seq and K562 PRO-seq can be found at the NCBI's Gene Expression Omnibus under accession number GSE184426 . (nature.com)
  • cAMP and cGMP may have differential roles in the regulation of γ-globin gene expression in erythroid cells. (elsevierpure.com)
  • Conclusions: There is potential improvement in PLD activity when co-administered with AHCC and decrease side effects of PLD. (researchgate.net)
  • Expression of γ-globin mRNA was examined by primer extension, and transcriptional activity of the γ-globin gene promoter was determined by reporter gene assays. (elsevierpure.com)
  • MDR1 mRNA appearance in addition to PGP in K562/Dox cells. (cancerrealitycheck.com)
  • In recent years, Massively Parallel Reporter Assays (MPRAs) have allowed to assess promoter or enhancer activity in thousands of DNA fragments en masse moving the genomics field forward ( 2 ). (biologists.com)
  • The formation of G-quadruplex DNA is easily conceivable in DNA sequences that are present as single strands in cells, such as the telomeric overhang. (hindawi.com)
  • The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)
  • Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)
  • However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
  • Strategies to circumvent resistance involve CRISPR/Cas9 gene editing to restore proper RNA splicing function in resistant cells. (osu.edu)
  • Background Several research show that multidrug transporters such as for example P-glycoprotein (PGP) get excited about cell level of resistance to chemotherapy and refractory epilepsy. (cancerrealitycheck.com)
  • These data obviously show that p38 reaches least partly mixed up in legislation of medication level of resistance in K562/Dox cells. (cancerrealitycheck.com)
  • PGP is really a transmembrane glycoprotein working as a medication transport that positively pumps out a number of anti-cancer agencies as well as other hydrophobic substances through the cells [7 20 hence reducing intracellular medication concentrations and resulting in medication resistance [10]. (cancerrealitycheck.com)
  • PGP-overexpressing K562/Dox cells allow us to study the effect of p38 inhibitor on drug resistance. (cancerrealitycheck.com)
  • K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes and are easily killed by natural killer cells as they lack the MHC complex required to inhibit NK activity. (wikipedia.org)
  • The expanded NK cells were tested for their ability to inhibit WNV infection in vitro. (fda.gov)
  • Agonist activity at alpha5beta1 in human K562 cells assessed as increase in cell adhesion to fibronectin pre-incubated for 30 mins before fibronectin. (bindingdb.org)
  • Agonist activity at alpha5beta1 integrin in human K562 cells assessed as increase of fibronectin-mediated cell adhesion after 30 mins by fluorescence. (bindingdb.org)
  • Dendrite pruning requires the developmentally regulated degradation of the cell adhesion molecule Neuroglian , and loss of Rab11 causes defects in the developmental degradation of Neuroglian and another target, the ion channel Ppk26 . (sdbonline.org)
  • Serum amyloid A medicates the inhibitory effect Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells. (shen-nong.com)
  • The correlation between the predictions and the guide activity varied considerably, especially for zebrafish. (biomedcentral.com)
  • Indeed, the identification of Kcnq1ot1, which targets Polycomb complexes to repressed genomic domains, and the significant overlap of ncRNAs isolated via a conventional technique suggest that RedChIP represents the way forward for studies of RNA-protein-DNA interactions in live cells. (epigenie.com)
  • The examination of women for the activation of NK, allows specialized scientists to apply a targeted therapeutic approach in order to regulate the function of NK cells. (articlebiz.com)
  • These results support the evaluation of cord blood-derived NK cells as a potential immuno-therapeutic approach in acute leukemias. (biologynews.net)
  • Here, we discuss how research has driven cancer drug development in the past and describe how recent advances in biology, technology, our conceptual understanding of cell networks and removal of some roadblocks may facilitate therapeutic advances in the (hopefully) near future. (biomedcentral.com)
  • Our work demonstrates that RGENs are highly active even with imperfectly matched RNA-DNA interfaces in human cells, a finding that might confound their use in research and therapeutic applications. (cdc.gov)
  • Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)
  • Not so long ago it was shown (1) that derivatives of curcumin enhance telomerase activity, both directly and indirectly. (antiaging-systems.com)
  • An interesting study has shown that cycloastragenol not only promotes telomerase activity, but it may also be useful in reducing depressive symptoms (7). (antiaging-systems.com)
  • Thus, over the past decade telomeric G-quadruplex DNA has been thoroughly studied with an initial focus on the possible interferences with telomerase activity [ 25 ]. (hindawi.com)
  • Uba, S. Molecular docking and quantitative structure-activity relationship study of anticonvulsant activity of aminobenzothiazole derivatives. (ufv.br)
  • Given the complexity of human transcriptional regulation in vivo and the multitude of potential epigenetic mechanisms cooperating to regulate transcriptional activity, questions remain about the interplay of regulatory mechanisms in patients with CMML. (nature.com)
  • Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
  • The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
  • NSUN5-FTH1 axis promoted the growth of GC cells in part by the regulation of ferroptosis. (bvsalud.org)
  • The problem with K562 cells, and many other cancer cell types, is an overabundance of Aurora kinases. (wikipedia.org)
  • Stabilization of quadruplex architecture by small molecules is thus emerging as a potential anticancer approach since it is thought to interfere with oncogenic expression and telomeric maintenance in cancer cells [ 11 - 13 ]. (hindawi.com)
  • The AICR Lifestyle & Cancer Symposium addresses the most current and consequential issues regarding diet, obesity, physical activity and cancer. (aicr.org)
  • The Annual AICR Research Conference is the most authoritative source for information on diet, obesity, physical activity and cancer. (aicr.org)
  • Cancer Update Program - unifying research on nutrition, physical activity and cancer. (aicr.org)
  • We champion the latest and most authoritative scientific research from around the world on cancer prevention and survival through diet, weight and physical activity, so that we can help people make informed lifestyle choices to reduce their cancer risk. (aicr.org)
  • BACKGROUND: Natural Killer (NK) cells are a crucial component of the host innate immune system with anti-viral and anti-cancer properties. (fda.gov)
  • While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
  • Our aims were to elucidate whether T cells can induce galectin-9 secretion in human cancer cells derived from solid malignant tumors and whether this soluble form displays higher systemic immunosuppressive activity compared with the cell surface-based protein. (bmj.com)
  • Results We discovered that T lymphocytes induce galectin-9 secretion in various types of human cancer cells derived from solid malignant tumors. (bmj.com)
  • As an example, we studied the cooperation of galectin-9 and V-domain Ig-containing suppressor of T cell activation (VISTA) proteins in human cancer cells. (bmj.com)
  • Cancer is a common term for a large group of disease which are characterized by uncontrolled cell growth and formation of tumors. (pdfplayer.org)
  • Cancer cells can spread to any part of the body and affecting individuals from different sexes, ages and races(American Cancer Society, 2020). (pdfplayer.org)
  • Importantly, vast amounts of information about features distinguishing tumor from normal cells is being accumulated, resulting in frequent, major new insights into cancer biology. (biomedcentral.com)
  • It is widely accepted, although clouded by the secrecy of war [ 2 ], that the first tentative steps to treating cancer with drugs emanated from the observation that exposure to chemical warfare agents ('poison gases'), such as nitrogen mustards, could limit the proliferative nature of rapidly dividing lymphoid cells. (biomedcentral.com)
  • T cells play a critical role in the control of cancer. (aacrjournals.org)
  • The development of immune checkpoint blockers (ICB) aimed at enhancing antitumor T-cell responses has revolutionized cancer treatment. (aacrjournals.org)
  • Traditionally, B cells have been believed to be the lymphocytes responsible for antibody production via maturation into plasma cells (ie, humoral immunity), and T cells have been believed to be the lymphocytes responsible for killing other cells or organisms (ie, cellular immunity). (medscape.com)
  • Currently, certain T lymphocytes (ie, T-helper cells) are known to be responsible for helping immature B cells develop into mature B cells. (medscape.com)
  • 1] Deoxyguanosine and deoxyadenosine are more toxic in lymphocytes than in other cell types. (medscape.com)
  • NK cells are a subpopulation of lymphocytes that express the CD56 + antigen on their cell surface. (articlebiz.com)
  • Background Galectin-9 is a member of the family of lectin proteins and crucially regulates human immune responses, particularly because of its ability to suppress the anticancer activities of T lymphocytes and natural killer cells. (bmj.com)
  • FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
  • Artemis deficiency (with mutations in the Artemis protein resulting in defective VDJ recombination) decreases both T cells and B cells and can be considered part of a subset of SCIDs. (medscape.com)
  • 2 Protein interactions are critical to the stability, localization and activity of b-catenin, and we recently performed the first proteomic analyses of the b-catenin interactome in myeloid cells. (haematologica.org)
  • 3 This study identified Wilms tumor protein (WT1) as a putative novel interaction partner in myeloid cells. (haematologica.org)
  • In order to idenitfy appropriate cell lines in which to study b-catenin:WT1 interplay we first performed a screen of myeloid cell lines to examine b-catenin and WT1 protein expression. (haematologica.org)
  • WT1 is an RNA-binding protein, 6 and β-catenin has also been shown to bind RNA, 7 so to confirm this interaction was not indirect via RNA binding we repeated WT1 Co-IP (with or without CHIR99021) after first confirming complete digestion of RNA through RNase A pre-treatment of cell lysates ( Figure 1E ). (haematologica.org)
  • Stimulated by the pioneering studies on the cationic porphyrin TMPyP4 and the natural product telomestatin, follow-up studies have developed, rapidly leading to a large diversity of macrocyclic structures with remarkable-quadruplex binding properties and biological activities. (hindawi.com)
  • Besides it provides a valuable snapshot of the chemical structure-activity relationship of compounds, highlighting the presence or absence of some specific atoms and chemical units in the structure of compounds can greatly influence their biological activities. (frontiersin.org)
  • 30,000 SNPs that altered enhancer activity mostly in a cell-type specific manner . (biologists.com)
  • The authors overcame MPRA size limitations to study mammalian large genomes and they were able to provide a whole-genome snapshot of enhancer activity. (biologists.com)
  • They also i ntegrated enhancer activity data with eQTLs and GWAS information helping prioritization of candidate causal genes. (biologists.com)
  • This means that these compounds may increase or reduce the activities of telomerase. (antiaging-systems.com)
  • Four types of molecular descriptors were generated in other to know the relationship that exit between anti-ulcer activity and structural properties of these compounds. (ufv.br)
  • and R 2 pred= 0.7975, our QSAR model showed an excellent predictive activity with chemical property of the compounds, the results of the docking analysis revealed that most of the compounds show very good relation with the active receptor, with a better docking score of -9.1kcal/mol. (ufv.br)
  • However, unlike the PMEA derivatives, the antimetabolites remained equally cytostatic for the mutant K562 cells and for the wild-type cells. (aspetjournals.org)
  • Secreted galectin-9 pre-opsonised T cells and, following interaction with other immune checkpoint proteins, their activity was completely attenuated. (bmj.com)
  • We further validated the interaction in two other myeloid cell lines (KG-1 and K562) ( Online Supplementary Figure S1A ) and confirmed that this interaction also occurs in the nucleus ( Figure 1D ). (haematologica.org)
  • Association between b-catenin and WT1 in myeloid cell lines. (haematologica.org)
  • Myeloid neoplasms are clonal hematopoietic stem cell disorders driven by the sequential acquisition of recurrent genetic lesions. (nature.com)
  • This arrest leads to "shrinkage, blebbing, nuclear fragmentation, chromatin condensing" and other morphological changes that cause the cell to program death at this point. (wikipedia.org)
  • These immature stem cells were easier to match to patients, especially those from non-Caucasian ethnicities, and could be stored for use as needed. (biologynews.net)
  • Triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 protease. (shen-nong.com)
  • The physicochemical parameters are to be considered when improving the inhibitory activities of benzo[d]thiazole against the enzyme that cause ulcer (H + /K + -ATPase). (ufv.br)
  • Western blot analysis, ELISA, quantitative reverse transcriptase-PCR, on-cell Western and other measurement techniques were used to conduct the study. (bmj.com)
  • A quantitative structure-activity relationship and molecular docking studies have been made on 30 benzo[d]thiazole series as H + /K + -ATPase inhibitors. (ufv.br)
  • The expression levels of miR-146b-5p and TRAF6 in CRC tissue and cells were determined by reverse transcription quantitative PCR and western blotting. (bvsalud.org)
  • Interestingly, NSUN5 regulated the levels of FTH1, instead of FTH1 regulating NSUN5 in GC cells. (bvsalud.org)
  • However, another study suggests that bcr:abl over-expression may actually increase cell adherence to cell culture plastic. (wikipedia.org)
  • However, the degree to which human enhancers and promoters are intrinsically compatible has not yet been systematically measured, and how their activities combine to control RNA expression remains unclear. (nature.com)
  • Stable cell lines were constructed on SGC7901 cells by using shRNAs and pcDNA3.1 expression vectors, respectively. (bvsalud.org)
  • In addition to providing evidence of the efficacy of AHCC, the mechanism of improved activity was also investigated. (researchgate.net)
  • GRN510, through a mechanism of telomerase activation, also suppressed the accumulation of senescent cells in the lung. (antiaging-systems.com)
  • As shown in Figure 1F-H, the b-catenin:WT1 interaction remained in K562, HEL and KG-1 cells under both basal (DMSO) and stimulated (CHIR99021) Wnt signaling. (haematologica.org)
  • Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)