ApoptosisMitotic cell cycleCheckpointsInhibitionRegulationInduce cell cycle arrestPhosphorylationProtein serineAssayFlow cytometryKinase activityInduced cell cycle arrestDisruptionMetastasisTranscriptionVitroReplicationCheckpointPhasesProgression through the cell cycleEnzyme activitySubstratesCytotoxic activityCyclin DependeFurther inhibitedCytotoxicityGrowthMolecularFACSGenesBiolAntitumorInhibitorCellularProteinsRenalMitosisProcessesPhaseCancerInhibitoryAngiogenesisMenstrualDegradationGeneExtracellularOccurMigrationExogenousChromatinApoptoticAntioxidant activitySurvival
Apoptosis29
- Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. (nih.gov)
- Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non-small cell lung cancer cells via p53-p21 signaling pathway. (nih.gov)
- Downregulation of CDCA5 Can Inhibit Cell Proliferation, Migration, and Invasion, and Induce Apoptosis of Prostate Cancer Cells. (nih.gov)
- Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
- In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
- These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
- The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
- Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
- Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
- Protein kinase B (PKB), also known as Akt, is the collective name of a set of three serine/threonine-specific protein kinases that play key roles in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. (wikipedia.org)
- Since it can block apoptosis and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. (wikipedia.org)
- Purified cannabinoids have been reported to prevent proliferation, metastasis, and induce apoptosis in a variety of cancer cell types. (nih.gov)
- Annexin V Assay and fluorescence-activated cell sorting (FACS) were used to determine apoptosis and cell cycle, and RNA sequencing was used to determine gene expression. (nih.gov)
- The F7 and F7+F3 cytotoxic activity involved cell apoptosis and cell cycle arrest in S or G0/G1 phases, respectively. (nih.gov)
- Activated p53 transcriptionally regulates hundreds of genes that are involved in multiple biological processes, including in DNA damage repair, cell cycle arrest, apoptosis and senescence. (nih.gov)
- In this Review, we discuss the multiple molecular mechanisms of p53 regulation and how they modulate the induction of apoptosis or cell cycle arrest following DNA damage. (nih.gov)
- To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis. (jcpjournal.org)
- EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
- Cancer cells can grow unusually via limitless number of cell division and can avoid apoptosis. (jcpjournal.org)
- 6 , 7 Therefore, the inhibition of cancer cell proliferation by inducing cell cycle arrest and apoptosis is being an important preventive strategy against cancer. (jcpjournal.org)
- If cells containing damaged DNA were to divide, the errors would be transmitted to daughter cells, generating genomic instability and resulting in tumorigenesis or apoptosis . (tocris.com)
- Then, the relatively insensitive gastric cancer cell lines (MGC-803 and BGC-823) were treated with low concentrations of chidamide alone, bortezomib alone, or chidamide and bortezomib combination to detect the effects on cell proliferation, apoptosis, migration, and invasion. (dovepress.com)
- Compared with low-dose chidamide or bortezomib alone, the low-dose drug combination significantly inhibited the proliferation, migration, and invasion of MGC-803 and BGC-823 cells and induced apoptosis of the cells. (dovepress.com)
- Histone deacetylase (HDAC) plays a pivotal role in regulating the level of cellular DNA acetylation and participate in cell growth, differentiation, and apoptosis. (dovepress.com)
- These fragments may also be generated during DNA repair, cell death (apoptosis), and other processes. (medlineplus.gov)
- We summarized that four main organic sulfides in garlic, diallyl disulfide (DADS), diallyl trisulfide (DATS), S‑allylmercaptocysteine (SAMC) and allicin, may contribute to the regulation of tumor cell apoptosis, migration and the cell cycle. (spandidos-publications.com)
- The active ingredients in garlic oil correspond mainly to a family of organosulfur molecules, which selectively increase redox stress in cancer cells, leading to apoptosis and death ( 23 ). (spandidos-publications.com)
- In addition, it can stimulate apoptosis of cells containing damaged DNA. (medscape.com)
- We found that curcumin inhibited cell growth , enhanced apoptosis , induced cell cycle arrest and retarded cell invasion in pancreatic cancer cells . (bvsalud.org)
Mitotic cell cycle2
- and syncytial blastoderm mitotic cell cycle. (nih.gov)
- Cyclin dependent-kinase 2 (CDK2) plays important functions during the mitotic cell cycle and also facilitates several key events during germ cell development. (lu.se)
Checkpoints5
- d) investigation of cell-cycle checkpoints and replicative responses to the changing environment in the human host and to antimalarial drugs. (europa.eu)
- For a cell to progress through the cycle and replicate, it must pass through checkpoints between phases to ensure that DNA is replicated correctly and that chromosomes segregate. (tocris.com)
- These checkpoints may stop the cell cycle after DNA damage, loss of DNA replication or disruption of the mitotic spindle, in order for repair processes to take place. (tocris.com)
- Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)
- Many more investigations have been made on cells, sorted according to size, or artificially arrested at various cell cycle checkpoints. (frontiersin.org)
Inhibition8
- In whole cells, Dox treatment prevented p34cdc2 kinase dephosphorylation without altering its synthesis, and this effect was due to neither down-regulation of cdc25C nor inhibition of protein-tyrosine phosphatase activity. (aspetjournals.org)
- A good correlation was found between extent of DNA double-strand breaks and p34cdc2 kinase activity inhibition. (aspetjournals.org)
- It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
- This was associated with disruption of cell cycle-related events, including hyperphosphorylation and inhibition of p34 cdc2 kinase activity, phosphorylation of cdc25C, and alteration in cyclin B1 expression. (elsevierpure.com)
- The immediate partial restoration of respiration upon addition of membrane-permeant dimethyl-2- oxoglutarate to inhibited cells demonstrated the absence of 2OGdehydrogenase substrate post aminotransferase inhibition.Surprisingly, the AOA (bithionol) inhibition stopped after 72 hours of 5% O2 hypoxia, and respiration was fully recovered. (pulsus.com)
- Many potential anticancer mechanisms of these compounds were proposed, including the inhibition of cell proliferation, changes in enzyme activity and immune regulation ( 21 , 22 ). (spandidos-publications.com)
- Inhibition of Cell Survival by Curcumin Is Associated with Downregulation of Cell Division Cycle 20 (Cdc20) in Pancreatic Cancer Cells. (bvsalud.org)
- In the current study, we explore whether curcumin exhibits its anti- cancer function through inhibition of oncoprotein cell division cycle 20 (Cdc20) in pancreatic cancer cells . (bvsalud.org)
Regulation6
- Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
- and regulation of cell cycle process. (nih.gov)
- This activity regulation strategy could inspire research into high-efficiency and high-power TREC devices for practical low-grade heat harnessing. (nus.edu.sg)
- In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. (tocris.com)
- Cell Biochemistry & Function publishes original research articles and reviews on the mechanisms whereby molecular and biochemical processes control cellular activity with a particular emphasis on the integration of molecular and cell biology, biochemistry and physiology in the regulation of tissue function in health and disease . (wiley.com)
- PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
Induce cell cycle arrest2
- C. sativa compounds interact synergistically for cytotoxic activity against colon cancer cells and induce cell cycle arrest, apoptotic cell death, and distinct gene expression. (nih.gov)
- It specifically negatively regulates the PI3K-AKT signaling pathway to induce cell cycle arrest. (medscape.com)
Phosphorylation6
- 12 The activation of cyclin/CDK complex accelerates cell cycle progression, which are regulated by phosphorylation. (jcpjournal.org)
- The most typical kind of cell cycle phosphorylation is on H3's serine. (news-medical.net)
- A Hepatocellular carcinoma in humans HepG2 cells are forced to undergo Oxidative Phosphorylation (OXPHOS) when grown in aglycemic conditions with galactose and glutamine.These OXPHOS cells serve as a prototype for cancer cells' combined aerobic glycolysis and OXPHOS bioenergetics. (pulsus.com)
- Phosphorylation at CDK1, CDK4, and CDK9 sites on, respectively, protein phosphatase 1alpha, retinoblastoma protein, and RNA polymerase II is inhibited in neuroblastoma SH-SY5Y cells exposed to meriolins. (rcsb.org)
- The forskolin-induced current potentiation was greatly reduced in cells transfected with VR-1 mutants carrying point mutations at the predicted PKA phosphorylation sites. (jneurosci.org)
- Three sequential phosphorylation events on specific residues of p27, regulate the activity of these complexes and ultimately control cell cycle proliferation or arrest. (lu.se)
Protein serine1
- Enables cyclin-dependent protein serine/threonine kinase regulator activity. (nih.gov)
Assay5
- Ethanol extracts of C. sativa were analyzed by high-performance liquid chromatography and gas chromatograph/mass spectrometry and their cytotoxic activity was determined using alamarBlue-based assay (Resazurin) and tetrazolium dye-based assay (XTT) on cancer and normal colon cell lines and on dysplastic adenomatous polyp cells. (nih.gov)
- To examine the antioxidant activity of EESR, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay was performed. (jcpjournal.org)
- Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
- First, the sensitivity and IC 50 values of chidamide and bortezomib in several gastric cancer cell lines (MGC-803, BGC-823, SGC-7901, and MKN45) were measured using the CCK-8 assay. (dovepress.com)
- The uptake of the purified MWCNTs was evaluated using fluorescence activated cell sorting (FACS), while the effects on cell fate were assessed using a 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt colorimetric assay, cell cycle and nanoindentation. (cdc.gov)
Flow cytometry1
- The distribution of the cell cycle was analyzed by flow cytometry. (fluoridealert.org)
Kinase activity3
- In untreated control P388 cells, p34cdc2 kinase localizes in the nucleus and cytoplasms, particularly in the centrosomes, and p34cdc2 kinase activity is dependent on cell cycle progression, with the enzyme activity increasing steadily from G1/S to G2/M and markedly declining thereafter. (aspetjournals.org)
- In a cell-free system, Dox did not inhibit p34cdc2 kinase activity, indicating that is has no direct effect on the enzyme function. (aspetjournals.org)
- Activated Akt isoforms can then go on to activate or deactivate their myriad substrates (e.g. mTOR) via their kinase activity. (wikipedia.org)
Induced cell cycle arrest2
- EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
- Our results showed that exposure to MWCNTs reduced cell metabolic activity and induced cell cycle arrest. (cdc.gov)
Disruption1
- Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)
Metastasis3
- Breast cancer stem cells (BCSCs) play a critical role in chemoresistance, metastasis, and poor prognosis of breast cancer. (hindawi.com)
- is known to exert anticancer effects, such as inducing cell cycle arrest, inhibiting metastasis, and overcoming immunotherapy resistance in breast cancer cells. (hindawi.com)
- Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
Transcription7
- The aryl hydrocarbon receptor (AhR) transcription factor is increasingly recognized as functioning in cell cycle control. (aspetjournals.org)
- Here we reconcile these results and show that whereas many commonly used cell lines do not exhibit a G1 R, primary fibroblasts have a G1 R that is defined by a precise Cdk activity threshold and the activation of cell-cycle-dependent transcription. (ebi.ac.uk)
- Specifically, we discuss how the interaction of p53 with DNA and chromatin affects gene expression, and how p53 post-translational modifications, its temporal expression dynamics and its interactions with chromatin regulators and transcription factors influence cell fate. (nih.gov)
- Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
- Therefore, NPAT could be the link between cell cycle machinery and shared heightened transcription of histone genes during S phase. (news-medical.net)
- pRB functions as a negative regulatory transcription factor during the G1 to S phase cell cycle transition. (medscape.com)
- p53 is a transcription factor whose expression is increased by DNA damage and blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
Vitro3
- The nuclear-centrosome complex was isolated from interphase Chinese hamster ovary (CHO) cells, and, with exogenous brain tubulin as a source of subunits, the centrosome, while attached to the nucleus, was demonstrated to nucleate microtubule formation in vitro. (rupress.org)
- We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
- The primary remit of the journal is on mammalian biology both in vivo and in vitro but studies on the biochemistry and function of cells in situ are especially encouraged . (wiley.com)
Replication14
- Malaria parasites replicate inside the cells of their human host via 'schizogony', which is fundamentally different from conventional binary fission - the replication mode used by most cells from human cells to yeast cells. (europa.eu)
- In schizogony a single parasite first generates many nuclei via independent, asynchronous rounds of genome replication, prior to cytokinesis which is the physical division of the cell. (europa.eu)
- Thus, schizogony challenges some basic paradigms about DNA replication control, while gametogenesis demands a speed of DNA replication and cell division that is unprecedented in eukaryotic gametogenesis. (europa.eu)
- This project is elucidating the spatio-temporal dynamics of DNA replication in these contrasting cell cycles. (europa.eu)
- Aim 1: detailed characterisation, at the whole-cell level, of the asynchronous genome replication that occurs in schizogony and gametogenesis. (europa.eu)
- In this chapter, we mainly discuss the coordination regulations between DNA replication initiation and other cell cycle events that ensure genomic integrity. (intechopen.com)
- DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)
- Due to their affiliation with DNA, histones are important for successful cell replication, which takes place via the cell cycle. (news-medical.net)
- Histones can be classified as replication dependent or replication independent, which is decided by their expression pattern during the cell cycle. (news-medical.net)
- The replication independent histone genes are transcribed at a relatively constant low rate, regardless of cell cycle stage. (news-medical.net)
- However, most of the vertebrate histone genes are replication dependent and are therefore more highly expressed during the cell cycle's S phase. (news-medical.net)
- 5 Nucleated cells divide after a sequence of seven phases: initial growth-G 2 , chromosomal replication-S, secondary growth-G 2 , prophase, metaphase, anaphase, and telophase. (icr.org)
- In this way, it breaks down unneeded DNA molecules or fragments that may be generated during copying (replication) of cells' genetic material in preparation for cell division. (medlineplus.gov)
- Standard errors which take into account the complex sample design of the NSFG have been estimated for Cycle IV using the balanced repeated replication technique. (cdc.gov)
Checkpoint2
- Checkpoint control kinases is a term used to describe a group of enzymes that regulate progression of a cell through the cell cycle. (tocris.com)
- Checkpoint control kinases function to halt the cell cycle at these critical points. (tocris.com)
Phases5
- The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
- The cell cycle consists of four phases (G1, S, G2 and M), all of which are characterized by different factors. (news-medical.net)
- Individual unsynchronized cells from three different cell lines were collected in different cell cycle phases (G0/G1 - S - G2/M) with variable cell sizes. (frontiersin.org)
- Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
- Various phases of activity within a cell leading to division are called the cell cycle. (icr.org)
Progression through the cell cycle3
- During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
- Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
- We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
Enzyme activity2
- In this study, we report that surface expression of CD26, through its associated DPPIV enzyme activity, enhanced sensitivity of Jurkat T-cell transfectants to G 2 -M arrest induced by the chemotherapeutic drug, doxorubicin. (elsevierpure.com)
- Glucose rapidly increased DAG content and PKC enzyme activity in microsomes, but not in plasma membranes or other membranes, during a 30-min treatment of rat adipocytes. (jci.org)
Substrates1
- Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
Cytotoxic activity1
- The unheated cannabis extracts (C2F), fraction 7 (F7), and fraction 3 (F3) had cytotoxic activity on colon cancer cells, but reduced activity on normal colon cell lines. (nih.gov)
Cyclin Depende1
- As a biologically important example we have studied the complex formed by cyclins and cyclin-dependent kinases (CDKs), which play an essential role in the control of the eukaryotic cell cycle. (lu.se)
Further inhibited1
- Further, the results of miR-4755-5p mimic transfection confirmed that under the action of NaF, miR-4755-5p overexpression reduced Cyclin D1 protein expression within osteoblasts and further inhibited cell proliferation and activation. (fluoridealert.org)
Cytotoxicity1
- We studied the effect of doxorubicin (Dox) on cell cycle progression and its correlation with DNA damage and cytotoxicity in p53-mutant P388 cells. (aspetjournals.org)
Growth10
- Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
- In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
- This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. (wikipedia.org)
- R has been viewed as a point in G1 that is passed when growth factor signaling initiates a positive feedback loop of Cdk activity. (ebi.ac.uk)
- EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
- The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)
- Such data are only indirectly related to quantitative changes in cells at different states of division and growth. (frontiersin.org)
- In studies using animals or cells grown in labs, cinnamon has shown promise for its ability to slow cancer growth and even kill tumor cells. (webmd.com)
- At a fundamental level, cancer is caused by an accumulation of genetic changes that result in unregulated cell growth and proliferation. (medscape.com)
- DSN: CC37.NSFG88 ABSTRACT Introduction The National Survey of Family Growth (NSFG), Cycle IV, was conducted in January through August of 1988 by Westat, Inc., under contract to the National Center for Health Statistics (NCHS). (cdc.gov)
Molecular6
- Taken together, these findings delineate AR function in mitotically active tumor cells, thus providing critical insight into the molecular basis by which AR promotes development of lethal PCa and nominate new avenues for therapeutic intervention. (ox.ac.uk)
- The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
- The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)
- However, the molecular mechanisms of action of HNK in BCSCs, as well as its effects on the cell cycle, remain unclear. (hindawi.com)
- This study aimed to explore the potential targets and molecular mechanisms of HNK on metastatic BCSC (mBCSC)-cell cycle arrest and the impact of the TME. (hindawi.com)
- Finally, the interaction between HNK and two HNK-targets regulating the cell cycle was analyzed using molecular docking analysis. (hindawi.com)
FACS1
- Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
Genes5
- Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
- In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
- Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
- The intersection between the differentially expressed genes (DEGs) and the HNK-targets was determined using a Venn diagram, and the results were analyzed using a protein-protein interaction network, hub gene selection, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, genetic alteration analysis, survival rate, and immune cell infiltration levels. (hindawi.com)
- Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
Biol2
- J Cell Biol (1981) 91 (3): 822-826. (rupress.org)
- J Cell Biol, 2007 Jun 18. (nih.gov)
Antitumor5
- The aim of this study was to investigate the antitumor effect of chidamide in combination with bortezomib on gastric cancer cell lines. (dovepress.com)
- Meriolins thus constitute a new CDK inhibitory scaffold, with promising antitumor activity, derived from molecules initially isolated from marine organisms. (rcsb.org)
- Bladder Cancer Extracellular Vesicles Elicit a CD8 T Cell-Mediated Antitumor Immunity. (rochester.edu)
- Importantly, the potential tumor‑preventing effect of these garlic ingredients warrants further investigation in regards to the specific mechanism of the underlying antitumor activities. (spandidos-publications.com)
- Curcumin , a polyphenol derived from the Curcuma longa plant , has been reported to exert its antitumor activity in pancreatic cancer . (bvsalud.org)
Inhibitor2
- Treating serum-released 5L cells with the alkyne metabolism-based P4501A1 inhibitor 1-(1-propynyl)pyrene results in prolonged AhR activation, enhanced p27 Kip1 expression, and G 1 phase arrest after serum release. (aspetjournals.org)
- LY2606368 is a potent and selective ATP competitive inhibitor(IC50=1.5 nM in SW1990 cell) of the Chk1 protein kinase. (chemspider.com)
Cellular2
- Our analysis further emphasized that the MWCNTs-induced cellular fate result from multiple types of interactions that could be analyzed by means of intracellular biomechanical changes and are pivotal in understanding the underlying MWCNT-induced cell transformation. (cdc.gov)
- Once in the cell, they alter cellular activity to turn the individual cells into "nurse cells. (medscape.com)
Proteins1
- The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)
Renal2
- Clinical hallmarks of VHL disease include the development of retinal and central nervous system (CNS) hemangioblastomas (blood vessel tumors), pheochromocytomas , multiple cysts in the pancreas and kidneys, and an increased risk for malignant transformation of renal cysts into renal cell carcinoma. (medscape.com)
- Early identification of VHL is important because of the increased risk of serious complications (eg, renal cell carcinoma) to foster more effective treatment options and better prognoses. (medscape.com)
Mitosis1
- Nucleating activity did not change discernibly throughout interphase but increased approximately fivefold at the transition to mitosis. (rupress.org)
Processes1
- Several of these processes change during the cell cycle, but unlike transcriptional changes, the post-translational changes occur throughout the cell cycle. (news-medical.net)
Phase12
- Several recent reports have shown that AhR activity in the absence of exogenous agonists or presence of the prototypical ligand 2,3,7,8-tetrachlorodibenzo- p -dioxin can affect G 1 phase progression in cultured cells. (aspetjournals.org)
- Serum release of serum-starved (G 0 ) 5L rat hepatoma cells triggers transient AhR activation and P4501A1 protein expression concomitant with the G 0 /G 1 -to-S phase transition. (aspetjournals.org)
- In contrast, sustained AhR activation in response to TCDD treatment increases p27 Kip1 expression in addition to P4501A1, resulting in G 1 phase cell cycle arrest. (aspetjournals.org)
- The data are consistent with a cell cycle role for P4501A1 because they show that P4501A1 negatively regulates the duration of AhR action through the metabolic removal of the receptor agonist, thereby preventing AhR-mediated G 1 phase arrest. (aspetjournals.org)
- Treatment of synchronized cells in early S phase with 1 microM Dox (2 x ID50) for 1 hr induced a marked cell arrest at G2/M phase at 6-12 hr after drug incubation. (aspetjournals.org)
- Catastrophic genetic damage can occur if cells progress to the next phase of the cell cycle before the previous phase is properly completed. (tocris.com)
- Upregulation of histone gene expression is one of the hallmarks of entry into the cell cycle's S phase. (news-medical.net)
- This process is assumed to be under the control of the cell cycle, because of how closely linked to the S phase histone synthesis is. (news-medical.net)
- Entry into the S phase in animal cells is regulated to a large extent by the cyclin E-CDK2 kinase complex. (news-medical.net)
- By applying the random forests algorithm, a supervised machine learning approach, we show how a multi-gene signature that classifies individual cells into their correct cell cycle phase and cell size can be generated. (frontiersin.org)
- Cyclin-dependent kinase 4 is a key regulator of G1 PHASE of the CELL CYCLE. (bvsalud.org)
- [ 8 ] Additionally, the normal cell life cycle of the invaded muscle cell enters a permanent arrest, with nuclear DNA remaining for the remainder of the life of the host at the G 2 /M phase transition. (medscape.com)
Cancer16
- Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
- Cell cycle-coupled expansion of AR activity promotes cancer progression. (ox.ac.uk)
- The androgen receptor (AR) is required for prostate cancer (PCa) survival and progression, and ablation of AR activity is the first line of therapeutic intervention for disseminated disease. (ox.ac.uk)
- This study was aimed to specify the cytotoxic effect of C. sativa -derived extracts on colon cancer cells and adenomatous polyps by identification of active compound(s) and characterization of their interaction. (nih.gov)
- Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
- Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. (tocris.com)
- It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death. (tocris.com)
- Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
- Chemoresistance, or the insensitivity of cancer cells to drug therapy, is a major factor in the failure of chemotherapy against breast cancer. (hindawi.com)
- Breast cancer stem cells (BCSCs) are one of the main factors driving chemoresistance, thereby contributing to poor prognosis and clinical outcomes [ 3 - 5 ]. (hindawi.com)
- It was our goal to identify the portions of the glutaminolytic pathway that involved the aminotransferase reaction that supplied 2-oxoglutarate (2OG) to the Krebs cycle active segment with Aconitase And Isocitrate Dehydrogenase-3 (ACO-IDH3), which is typically inactive in cancer cells because citrate is exported from the mitochondria. (pulsus.com)
- Thus, under conditions frequently established for solid tumours in vivo, such as aglycemia and hypoxia, the metabolic flexibility of cancer cells is demonstrated. (pulsus.com)
- Assessing the Magnitude of Immunogenic Cell Death Following Chemotherapy and Irradiation Reveals a New Strategy to Treat Pancreatic Cancer. (rochester.edu)
- Moreover, we observed that curcumin significantly inhibited the expression of Cdc20 in pancreatic cancer cells . (bvsalud.org)
- Furthermore, our results demonstrated that overexpression of Cdc20 enhanced cell proliferation and invasion, and abrogated the cytotoxic effects induced by curcumin in pancreatic cancer cells . (bvsalud.org)
- Dans une étude cas-témoins non appariés en milieu hospitalier, 108 personnes atteintes d'un cancer et 105 témoins ont été interrogés sur leurs apports alimentaires en recourant à la version en langue arabe d'un questionnaire validé sur la fréquence de consommation alimentaire. (who.int)
Inhibitory1
- Finally, the inhibitory effect of the combined chidamide and bortezomib treatment on MGC-803 cells was verified in vivo through tumor formation experiments in nude mice. (dovepress.com)
Angiogenesis1
- To nourish themselves in the nurse cell, the larvae stimulate angiogenesis leading to formation of a capillary rete around the invaded muscle cell. (medscape.com)
Menstrual1
- The first reference found related to the therapeutic use of the plant data from 2700 B.C. and is present in the pharmacopoeia of the Chinese Emperor Shen-Nung, where this plant was recommended in the treatment of malaria, rheumatic pain, in irregular and painful menstrual cycles. (bvsalud.org)
Degradation2
- In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)
- Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
Gene3
- Analyzing AR activity as a function of cell cycle revealed an unexpected and highly expanded repertoire of AR-regulated gene networks in actively cycling cells. (ox.ac.uk)
- Illustrating the impact of these unmasked AR-driven pathways, dihydroceramide desaturase 1 was identified as an AR-regulated gene in mitotically active cells that promoted pro-metastatic phenotypes, and in advanced PCa proved to be highly associated with development of metastases, recurrence after therapeutic intervention and reduced overall survival. (ox.ac.uk)
- Phenotypic variation in Aicardi-Goutieres syndrome explained by cell-specific IFN-stimulated gene response and cytokine release. (medlineplus.gov)
Extracellular1
- Active cells secreted more extracellular polymeric substances and oxidized lignin more strongly. (lu.se)
Occur1
- These changes in repolarization do not occur in all myocardial cells. (medscape.com)
Migration1
- Our results suggest that the combination of chidamide and bortezomib can significantly reduce the proliferation, invasion, and migration of MGC-803 and BGC-823 cells, providing a framework for the clinical evaluation of combined therapies for gastric cancers. (dovepress.com)
Exogenous1
- In addition, we demonstrate that the addition of exogenous soluble DPPIV enhanced sensitivity of lymphoid tumor cell lines to doxorubicin, suggesting a potentially useful clinical role for CD26/DPPIV in the treatment of selected human hematological malignancies. (elsevierpure.com)
Apoptotic1
- A small increase in early and late apoptotic events in irradiated MCF-7 cells was observed. (bvsalud.org)
Antioxidant activity1
- The anticancer model's overal antioxidant activity (34.72 µg/mL) was slightly lower than quercetin (30.44 µg/mL) but higher than ascorbic acid (41.68 µg/mL). (who.int)
Survival1
- Drosophila CIAPIN1 homologue is required for follicle cell proliferation and survival. (nih.gov)