AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureInformation ScienceHealth Care
HydrolasesGlycoside HydrolasesEpoxide HydrolasesCarboxylic Ester HydrolasesLysosomesAcid PhosphataseGlucuronidaseHexosaminidasesMannosephosphatesN-Acetylmuramoyl-L-alanine AmidasePeptide HydrolasesMucolipidosesMolecular Sequence DataSubstrate SpecificityThiolester HydrolasesReceptor, IGF Type 2Amino Acid SequenceAcetylglucosaminidasealpha-L-FucosidaseHydrolysisXylosidasesCellulaseCellvibriobeta-GlucosidaseGalactosidasesbeta-MannosidaseEndo-1,4-beta XylanasesXylansAcid Anhydride HydrolasesArylsulfatasesbeta-N-AcetylhexosaminidasesDisaccharidasesSequence Homology, Amino AcidPyrophosphatasesCathepsinsAmidohydrolasesGlucosidasesMannosidasesN-Glycosyl HydrolasesCathepsin DCell WallMonocrotophosCloning, MolecularTransferases (Other Substituted Phosphate Groups)CellulasesCatalytic DomainEsterasesKineticsVacuolesSequence AlignmentCatalysisCelluloseHydrogen-Ion ConcentrationBacterial ProteinsSaposinsAcetylesteraseCellulose 1,4-beta-CellobiosidaseSulfatasesModels, MolecularSucraseSterol EsterasePhosphoric Monoester HydrolasesGlucansalpha-Mannosidasealpha-GlucosidasesEnzyme StabilityCathepsin AOligosaccharidesCrystallography, X-RayPalmitoyl-CoA HydrolasePhylogenyNeocallimastigalesEscherichia coliAlteromonasAspartylglucosylaminaseFibrobacterGlucan Endo-1,3-beta-D-GlucosidasePhosphoric Diester HydrolasesEpoxy CompoundsRecombinant ProteinsSequence Analysis, DNABacteriolysisCarboxylesterasePeptidoglycanBinding SitesGlucan 1,4-beta-GlucosidasePiromycesPolysaccharidesChitinaseAspergillus nigerGlycosyltransferasesCarboxypeptidasesUbiquitin ThiolesteraseBase SequenceLeucyl AminopeptidaseEndopeptidasesCathepsin BTrichodermaSphingolipid Activator ProteinsHexosephosphates
Glycoside hydrolasesEnzymeEnzymesCatalyticPeptide hydrolasesMetabolismProteinSoluble Epoxide HInhibitorsLysosomal hydrolasesCharacterizationOrganic SynthesisPutativeInhibitory activityAntibodyDeficiencyGeneFamilyHydrolysisLeukotrieneCompoundsSubstratesAssaySterolLiverStructuresSpecificitySpecificallyHydrolyticAbstractHumanAcid
Glycoside hydrolases8
  • In molecular biology, glycoside hydrolase family 92 is a family of glycoside hydrolases. (wikipedia.org)
  • Glycoside hydrolases EC 3.2.1. (wikipedia.org)
  • A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. (wikipedia.org)
  • Functional, biochemical, and preliminary structural properties are reported for three glycoside hydrolases of the recently described glycoside hydrolase (GH) family 159. (rcsb.org)
  • The genome of Rhodothermus marinus DSM 4253 encodes six glycoside hydrolases (GH) classified under GH family 3 (GH3): RmBgl3A, RmBgl3B, RmBgl3C, RmXyl3A, RmXyl3B and RmNag3. (nih.gov)
  • We show that CcsZ is in fact capable of this activity and belongs to a broader family of glycoside hydrolases with unexpected taxonomic diversity. (biorxiv.org)
  • The research focus is within the area "enzymes of extremophilic microorganisms", especially concerning thermostable glycoside hydrolases (including representatives from GH-family 1, 3, 10, 12 and 13). (lu.se)
  • 5. Rather, MY, Nordberg Karlsson E, Adlercreutz P. ( 2015 ) Complexation of alkyl glycosides with α-cyclodextrin can have drastically different effects on their conversion by glycoside hydrolases. (lu.se)
Enzyme16
  • The bifunctional enzyme soluble epoxide hydrolase (sEH) is found in all regions of the brain. (frontiersin.org)
  • Summary of studies investigating the roles of the carboxy- and the amino terminal domains of the soluble epoxide hydrolase enzyme. (frontiersin.org)
  • FAAH belongs to the serine hydrolase enzyme family. (tocris.com)
  • The 3D structure and its substrate-binding pocket also provides an explanation at the molecular level for the observed exo-activity of the enzyme. (rcsb.org)
  • These results provide evidence for the secretion of a bile salt-dependent CEH activity, from both a hepatic cell line and the intact liver, that has similar properties to the enzyme previously isolated from rat liver homogenates and rat pancreas. (psu.edu)
  • This is the first report of a dual activity enzyme, highly conserved in land plants, which catalyzes the hydrolysis of a peptide bond and of a phosphate bond, acting both as a dipeptidyl peptidase III and an atypical Nudix hydrolase. (irb.hr)
  • Unlike the rat and mouse enzymes, which require DTT for maximal activity, the human enzyme is DTT-independent. (novusbio.com)
  • Leukotriene A 4 hydrolase (LTA 4 H) is a bi-functional enzyme that promotes a pro-inflammatory epoxy hydrolase (EH) pathway and an anti-inflammatory aminopeptidase (AP) pathway. (gmu.edu)
  • In conclusion, our research involves the high-throughput assay for screening small molecules to modulate the AP activity of LTA 4 H. The discovery of small molecules that activate the AP activity of the LTA 4 H enzyme is a potential new therapeutic agent for lung inflammatory diseases, such as chronic obstructive pulmonary disease (COPD). (gmu.edu)
  • Reliance on plant GH32 enzyme activity for C acquisition in these symbionts supports earlier predictions of possible plant control over C allocation in the mycorrhizal symbiosis. (elsevierpure.com)
  • The AHCY gene provides instructions for producing the enzyme S-adenosylhomocysteine hydrolase. (medlineplus.gov)
  • Most of these variants substitute one amino acid for another amino acid in the S-adenosylhomocysteine hydrolase enzyme, causing it to process methionine less efficiently. (medlineplus.gov)
  • Other variants introduce a premature stop signal in the instructions for making the S-adenosylhomocysteine hydrolase enzyme. (medlineplus.gov)
  • These changes reduce the activity of the S-adenosylhomocysteine hydrolase enzyme, impairing the breakdown of methionine. (medlineplus.gov)
  • Thermo Scientific Active Site Probes covalently bind to the active sites of kinases, GTPases and serine hydrolases to enable selective enrichment and profiling of target enzyme classes. (thermofisher.com)
  • Thermo Scientific Pierce Active Site Probes are chemical probes that covalently bind to the active sites of specific enzyme classes such as kinases, GTPases, and serine hydrolases. (thermofisher.com)
Enzymes11
  • Glycoside hydrolase family 92 includes enzymes with mannosyl-oligosaccharide α-1,2-mannosidase EC 3.2.1.113, mannosyl-oligosaccharide α-1,3-mannosidase EC 3.2.1. (wikipedia.org)
  • Observing the changes of activity of some lysosomal enzymes in blood serum of female rabbits subjected to injection of 10 microg of ghrelin/kg of body weight. (nel.edu)
  • As a result of ghrelin injection the activity of all the enzymes examined in blood serum increased markedly. (nel.edu)
  • Changes of lysosomal enzymes activities in the blood serum caused by the effects of ghrelin should be regarded as the response of the lysosomal system. (nel.edu)
  • Photosynthate within plants is transported from source to sink tissues as sucrose, which is hydrolyzed by plant glycosyl hydrolase family 32 enzymes (GH32) into its constituent monosaccharides to meet plant cellular demands. (elsevierpure.com)
  • Microbial enzymes have a wide range of metabolic activities and are involved in the transformation of pollutants. (igi-global.com)
  • All active site probes can be used to determine inhibition of enzymes by small molecules, and some probes also preferentially react with only active enzymes, allowing for activity-based proteomic profiling (ABPP). (thermofisher.com)
  • 3 Since these reagents only label active enzymes, they are able to monitor enzymatic activity in addition to being used for studying inhibitor binding affinities. (thermofisher.com)
  • The serine hydrolase family includes a variety of enzymes such as cholinesterases, hydrolases, lipases, and proteases like trypsin, kallikreins and dipeptidyl peptidases. (thermofisher.com)
  • Since many of these enzymes are expressed as inactive pro-proteins, the ability of these probes to assess activity is especially advantageous over traditional protein or RNA expression profiling techniques which only measure abundance. (thermofisher.com)
  • They are challenging enzymes to express recombinantly as parts of the proteome can be hydrolyzed by their activity. (lu.se)
Catalytic5
  • It has two different catalytic activities, each assigned to one of its terminal domains: the C-terminal domain presents hydrolase activity, whereas the N-terminal domain exhibits phosphatase activity. (frontiersin.org)
  • This review discusses the function of sEH in mammals and its protein structure and catalytic activities. (frontiersin.org)
  • This review aims to summarize the role of sEH in mammals and its protein structure and catalytic activities. (frontiersin.org)
  • The purified recombinant catalytic domain of Acp displayed lytic activity on the cell walls of several Gram-positive bacterial species. (pasteur.fr)
  • does not seem to have catalytic activity. (hmdb.ca)
Peptide hydrolases1
Metabolism1
  • S-adenosylhomocysteine hydrolase deficiency in a human: a genetic disorder of methionine metabolism. (medlineplus.gov)
Protein9
  • Mechanism of 53BP1 activity regulation by RNA-binding TIRR and a designer protein. (nih.gov)
  • Fatty acid amide hydrolase , (FAAH, Oleamide hydrolase, Anandamide amidohydrolase), is an integral membrane protein that hydrolyzes bioactive amides, including anandamide , to free fatty acid and ethanolamine. (tocris.com)
  • Although bile salt-dependent CEH activity was measured in the medium at 6 to 96 h (up to 4500 pmol/h per mg cell protein), there was very little activity detected in the corresponding cell homogenates (less than 70 pmol/h per mg cell protein). (psu.edu)
  • Incubation of HepG2 cells with brefeldin A resulted in an 80 to 90% inhibition of secretion of the bile salt-dependent CEH activity, while only inhibiting total protein secretion by 42%, Bile salt-dependent CEH activity could also be detected in rat liver perfusates. (psu.edu)
  • In a search for plant homologues of dipeptidyl peptidase III (DPP III) family, we found a predicted protein from the moss Physcomitrella patens (UniProt entry: A9TLP4), which shared 61% sequence identity with the Arabidopsis thaliana uncharacterized protein, designated Nudix hydrolase 3. (irb.hr)
  • Both proteins contained all conserved regions of the DPP III family, but instead of the characteristic hexapeptide HEXXGH zinc-binding motif, they possessed a pentapeptide HEXXH, and at the N-terminus, a Nudix box, a hallmark of Nudix hydrolases, known to act upon a variety of nucleoside diphosphate derivatives.To investigate their biochemical properties, we expressed heterologously and purified Physcomitrella (PpND) and Arabidopsis (AtND) protein. (irb.hr)
  • 2020) [PMID=31595646] who demonstrated the b-N-acetylglucosaminidase activity of the GH109_Pool2 protein from an uncultured archaeon in a metagenomic sample. (cazy.org)
  • These products are based on Activity-Based Protein Profiling Technology, licensed from ActivX Biosciences, Inc. (thermofisher.com)
  • May be a single membrane channel protein acting both as a hydrolase and a translocase. (lu.se)
Soluble Epoxide H2
  • Development of on-line liquid chromatography-biochemical detection for soluble epoxide hydrolase inhibitors in mixtures. (cdc.gov)
  • In this study, an end-point-based fluorescence assay for soluble epoxide hydrolase (sEH) was transformed into an on-line continuous-flow format. (cdc.gov)
Inhibitors2
  • Previously published studies focused on blocking EH activity to inhibit the LTB 4 formation with small molecule inhibitors. (gmu.edu)
  • Grape seed and tea extracts and catechin 3-gallates are potent inhibitors of α-amylase and α-glucosidase activity. (oregonstate.edu)
Lysosomal hydrolases2
  • Effect of ghrelin on activities of some lysosomal hydrolases in rabbits. (nel.edu)
  • Sphingomyelinase follows the same intracellular targeting and posttranslational processes as most of the lysosomal hydrolases. (medscape.com)
Characterization1
Organic Synthesis1
  • Hydrolases in Organic Synthesis - Regio- and Stereoselective Biotransformations. (thieme-connect.com)
Putative1
  • To test this hypothesis, we subcloned the putative glycoside hydrolase CcsZ and solved the X-ray crystal structure of both apo- and product-bound CcsZ. (biorxiv.org)
Inhibitory activity1
  • DSpace at EWHA: Synthesis of apio analogues of neplanocin a and their inhibitory activity against S-adenosylhomocysteine hydrolase. (ewha.ac.kr)
Antibody1
  • There are currently no images for ADP-ribosylarginine hydrolase Antibody (NBP3-20168AF532). (novusbio.com)
Deficiency3
  • S-Adenosylhomocysteine hydrolase deficiency: a second patient, the younger brother of the index patient, and outcomes during therapy. (medlineplus.gov)
  • S-adenosylhomocysteine hydrolase deficiency in a 26-year-old man. (medlineplus.gov)
  • The Niemann-Pick group of diseases can be subclassified into two categories: (1) those with a primary deficiency in acid sphingomyelinase (ASM) activity (ie, types A and B) and (2) those with defective intracellular processing and transporting of low-density lipoprotein (LDL)-derived cholesterol (ie, type C). (medscape.com)
Gene1
  • Fumarylacetoacetate hydrolase gene as a knockout target for hepatic chimerism and donor liver production. (nih.gov)
Family2
  • Our results demonstrate that CcsZ is in fact an endo -acting cellulase belonging to glycoside hydrolase family 5 (GH-5). (biorxiv.org)
  • 2016) Construction of the first thermostable β-glucosynthase derived from glycoside hydrolase family 3. (lu.se)
Hydrolysis3
  • LTA 4 H EH activity catalyzes the hydrolysis of leukotriene A 4 (LTA 4 ) to the neutrophil chemoattractant leukotriene B 4 (LTB 4 ), while AP activity hydrolyzes the tripeptide proline-glycine-proline (PGP) to clear neutrophils in the lungs. (gmu.edu)
  • Incubation of liver microsomes with EC (1 mM) produced slight but significant decreases in NDMA demethylation and PNA hydrolysis activities. (aspetjournals.org)
  • The on-line BCD was based on a flow system wherein sEH activity was detected by competition of analytes with the substrate hydrolysis. (cdc.gov)
Leukotriene1
  • The cell-free media from MWCNT-polarized macrophages induced the migration of neutrophilic cells (differentiated from HL-60), which was blocked by Acebilustat, a specific leukotriene A4 hydrolase inhibitor, or LY239111, an LTB4 receptor antagonist, but not NS-398, a cyclooxygenase 2 inhibitor, revealing LTB4 as a major mediator of neutrophil chemotaxis from MWCNT-polarized macrophages. (cdc.gov)
Compounds2
  • Written by Roger Pertwee, this review discusses compounds which affect the activity of the endocannabinoid system, focusing particularly on ligands that are most widely used as experimental tools and denotes compounds available from Tocris. (tocris.com)
  • Specifically, S-adenosylhomocysteine hydrolase controls the step that converts the compound S-adenosylhomocysteine to the compounds adenosine and homocysteine. (medlineplus.gov)
Substrates4
  • Activity screening using a set of para -nitrophenyl ( p NP) glycosides suggested additional arabinosidase activity on substrates with arabinosyl residues, which has not been previously reported for members of GH159. (rcsb.org)
  • RmXyl3A and RmXyl3B showed β-xylosidase activity with different specificities for para-nitrophenyl (pNP)-linked substrates and xylooligosaccharides. (nih.gov)
  • Furthermore, PpND and AtND showed DPP III activity against dipeptidyl-2-arylamide substrates, which they cleaved with different specificity. (irb.hr)
  • Within a small sample, some isolates had crude activities comparable to that of Trichoderma reesei, particularly at lower temperatures and on more complicated biomass substrates. (cornell.edu)
Assay1
  • The protease presence was confirmed with a Western blot assay and activity was measured with an in vitro assay. (lu.se)
Sterol1
Liver4
  • Although there was measurable activity in all of 14 livers analyzed (47 ± 10 and 53 ± 17 nmol/h per g liver per h perfusion during two 5-min collections after 15 and 30 min of perfusion, respectively), it did not correlate with the activity found in corresponding liver homogenates, as only four livers had detectable bile salt-dependent CEH activity. (psu.edu)
  • however, reaction with PAX and not PMSF or EC resulted in loss of immunoreactivity for hydrolase B. These data correlated with levels of covalent binding of [ ethyl - 14 C]EC to liver microsomes, which were significantly elevated in incubations conducted with PAX or PMSF. (aspetjournals.org)
  • These results are consistent with the premise that EC is metabolized by CYP2E1 and hydrolase A in liver microsomes of mice. (aspetjournals.org)
  • 2 The antibodies for the carboxylesterases were raised against rat liver microsomal hydrolase A and hydrolase B. For ease of description, the murine liver microsomal proteins recognized by these antibodies are designated with the same nomenclature. (aspetjournals.org)
Structures1
Specificity1
  • Its hydrolytic specificity was established by analyzing the Bacillus subtilis peptidoglycan digestion products by coupling reverse phase-high-pressure liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, which displayed an N-acetylglucosaminidase activity. (pasteur.fr)
Specifically1
  • A. Fluorophosphonate probes covalently attach specifically to the active site of enzymatically active serine hydrolases and proteases. (thermofisher.com)
Hydrolytic2
  • The only activity previously reported for GH159 was hydrolytic activity on β-galactofuranosides. (rcsb.org)
  • Hydrolytic activity enhances the DSB early in the time course for fibers and throughout the time course for mats, indicating a significant effect of cellulose structure and accessibility on cellulase binding in a synergistic mixture. (cornell.edu)
Abstract1
  • abstract = "HepG2 cells and medium were assayed for cholesteryl ester hydrolase (CEH) activity in the presence and absence of sodium cholate. (psu.edu)
Human2
  • Particular attention was given to the distribution and expression of sEH in the human brain, deepening into the enzyme's phosphatase activity and its participation in brain cholesterol synthesis. (frontiersin.org)
  • We also provide a compilation of sEH distribution and expression in the human brain and deepen the discussion on the enzyme's N-terminal phosphatase activity and its involvement in the synthesis of cholesterol. (frontiersin.org)
Acid3
  • Tocris offers the following scientific literature for Fatty Acid Amide Hydrolase to showcase our products. (tocris.com)
  • In the blood serum the activity of cathepsins D and L, alanine aminopeptidase, acid phosphatase, lysosomal lipase and lysosomal esterase was determined. (nel.edu)
  • Finally, the technological characteristics (acidifying capacity, antimicrobial production, proteolytic activity, organic acid, and volatile organic compound generation) of the 50 LAB strains were investigated. (who.int)