Including Mast cellsDegranulationHuman basophils and mast cellsEosinophilsHistamine releaseAnaphylaxisMediatorsReceptorReactivityCD63Allergic reactionsAllergenTestInhibit the releaseGranulesStimulationAllergyMonocytesVitroAssaysMast cell activationDifferentiationAngioedemaAntibodyClinicallyInhibitionCellsInhibitsMechanismAntibodiesSpecificResultsExpressionSignificant
Including Mast cells1
- There are receptors (FcεR) for the constant region of IgE, the Fc region, on several types of cells, including Mast cells and Basophils. (wikipedia.org)
Degranulation9
- Basophils contain many granules inside the cell, which are filled with a variety of active substance triggering an allergic response upon degranulation. (wikipedia.org)
- The inner cell surface of the granules becomes the outer cell surface of the basophil /mast cell during degranulation process. (wikipedia.org)
- These reactions tend to be mediated by IgE, which differentiates them from non-IgE-mediated (formerly called anaphylactoid) reactions that involve IgE-independent mast cell and basophil degranulation. (medscape.com)
- Nonimmunologic anaphylaxis" is caused by agents or events that induce sudden, massive mast cell or basophil degranulation in the absence of immunoglobulins. (worldallergy.org)
- Any process that modulates the frequency, rate or extent of basophil degranulation. (planteome.org)
- Link to all annotated objects annotated to regulation of basophil degranulation. (planteome.org)
- Link to all direct and indirect annotations to regulation of basophil degranulation. (planteome.org)
- Mast cell degranulation and activation of Th2 cells triggered either by allergen-specific immunoglobulin E (IgE) or by alternative mechanisms, such as locally produced neurotransmitters, underlie the pathophysiological process of airway constriction during an asthma attack. (cdc.gov)
- When the specific antigen, or one similar to it, binds to the high affinity FcεRI-α receptor of the immunoglobulin, mast cell and basophil degranulation occurs. (medscape.com)
Human basophils and mast cells2
- My research has focused on the IgE receptor activation of human basophils and mast cells. (hopkinsmedicine.org)
- In these experiments we compared the effect of zinc chloride on the release of chemical mediators from human basophils and mast cells isolated from human lung. (nih.gov)
Eosinophils5
- CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ/δ T cells), monocytes, basophils, and eosinophils. (biolegend.com)
- It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. (rndsystems.com)
- IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. (rndsystems.com)
- The granulocytes include the neutrophils, basophils and eosinophils. (loinc.org)
- Eosinophils and basophils respond to allergic reactions and are capable of ingesting antigen-antibody complexes. (loinc.org)
Histamine release2
- Allergic symptoms are caused by an initial systemic histamine release by activated basophils and mast cells, that may lead to shock with laryngeal edema, lower-airway obstruction and hypotension. (wikipedia.org)
- Evidently, proton channels are active in basophils during IgE-mediated responses and prevent excessive acidification, which may account for their role in histamine release. (johnshopkins.edu)
Anaphylaxis5
- He has served as the principal investigator and co-investigator for basic and translational studies examining the role of IgE receptor expression and activation in allergic airways disease, anaphylaxis, and chronic urticaria. (hopkinsmedicine.org)
- The World Allergy Organization defines "anaphylaxis" as an acute, potentially lethal, multisystem syndrome resulting from the sudden release of mast cell and basophil-derived mediators into the circulation. (worldallergy.org)
- One of the most important cells in studying this ailment are basophils, which activate inflammation and other allergic responses such as rashes and anaphylaxis. (stanford.edu)
- Many of the clinical presentations seen in anaphylaxis are due to activation of multiple histamine receptors. (medscape.com)
- Tryptase levels of 11.5 ng/mL or greater are indicative of either mast cell activation (as in anaphylaxis) or increased total mast cell levels (as in mastocytosis). (medscape.com)
Mediators2
- Type I reactions (i.e., immediate hypersensitivity reactions) involve immunoglobulin E (IgE)-mediated release of histamine and other mediators from mast cells and basophils. (medscape.com)
- The activation of these cells leads to the release of chemical mediators such as histamine and arachidonic acid metabolites, which increase vascular permeability and tissue swelling 9 . (bvsalud.org)
Receptor2
- However, stimulation of human basophils in perforated-patch configuration with PMA, N-formyl-methionyl-leucyl-phenylalanine, or anti-IgE greatly enhanced proton currents, the latter suggesting involvement of proton channels during activation of basophils by allergens through their highly expressed IgE receptor (FcεRI). (johnshopkins.edu)
- My recent studies focus on the mechanisms underlying chronic urticaria, where we found altered IgE-receptor signaling related to the phenotype of mast cells and basophils. (hopkinsmedicine.org)
Reactivity3
- Diagnostic testing included serum levels of Ses i 1-specific IgE, skin prick test with high-protein extract, and basophil reactivity (% induced CD63 expression) for each patient. (tau.ac.il)
- IgE reactivity and basophil activation studies were performed in clinically defined mugwort-allergic patients. (nih.gov)
- Mugwort-allergic patients and humanized mice sensitized by allergen inhalation showed IgE reactivity and/or basophil activation mainly to folded, complete Art v 1 but not to unfolded, sequential peptide epitopes. (nih.gov)
CD633
- As flow cytometry is a valuable tool for analyzing large numbers of cells and for identifying cell populations, even at low concentrations, the percentage of basophils activated after in vitro stimulation by allergens and expressing the CD63 marker can be determined. (wikipedia.org)
- Several minutes at room temperature gives the marker time to bind to the CD63 proteins on the cell membrane of the basophil. (wikipedia.org)
- At a bee venom concentration of 100 ng/mL, the mean proportion of basophils expressing CD63 was 56% in reactors and 13.2% in nonreactors (P=.0321). (jiaci.org)
Allergic reactions2
- The role of basophil activation test and component-resolved diagnostics in the workup of egg allergy in children at low risk for severe allergic reactions: A real-life study. (bvsalud.org)
- Developed by researchers at King's College London, the new test described in the JACI letter is based on the activation of mast cells, which are similar to basophils and play a key role in allergic reactions. (foodallergy.org)
Allergen2
- A test tube is prepared with basophil stimulation buffer (BSB) including Interleukin 3 and an allergen which is to be tested. (wikipedia.org)
- In a paper published in Lab on a Chip , the researchers outline a microfluidic system that isolates allergen-reactive basophils from blood samples. (stanford.edu)
Test11
- The aim of the study was to evaluate the presence of specific D. pteronyssinus (DP) activation of basophils by basophil activation test (BAT) in subjects with confirmed LAR. (biomedcentral.com)
- Our study aimed to evaluate the utility of the basophil activation test (BAT) and component-resolved diagnostic in the diagnostic workup of children with egg allergy . (bvsalud.org)
- We investigated usefulness of the basophil activation test (BAT) in predicting the outcome of sting challenge in bee venom allergic patients after VIT. (jiaci.org)
- We identified the basophil activation test as the most promising one given all the data from our clinical collaborators and labs throughout the world. (stanford.edu)
- Scientists have shown that a test that's been around for more than 10 years, the basophil activation test, is a lot more accurate than other allergy tests - like a skin prick test or traditional blood test - but it's currently only used in research settings. (stanford.edu)
- Currently, the basophil activation test requires a cumbersome laboratory procedure. (stanford.edu)
- The basophil activation test is just the first application of this device. (stanford.edu)
- This mast cell activation test was compared to other available diagnostic tests on the basis of sensitivity (the rate of true positive tests, in which a disease is successfully identified) and specificity (the rate of true negative tests, in which a disease that isn't present is successfully ruled out). (foodallergy.org)
- A less common and more complicated blood test, the basophil activation test (BAT), assesses whether exposure to food extract can stimulate basophils - a type of white blood cell - to produce proteins associated with allergic reaction. (foodallergy.org)
- This mast cell activation test (MAT) assesses the extent to which peanut-treated serum from patients' blood samples can trigger allergy-associated changes in commercially available cultured mast cells. (foodallergy.org)
- The immunological background of the sesame seed and tree nut IgE sensitization was characterized with Western blotting and a basophil activation test (BAT). (wur.nl)
Inhibit the release2
- We have previously shown that physiological concentrations of zinc (congruent to 7 X 10(-6) M) inhibit the release of histamine from human basophil leukocytes (Marone et al. (nih.gov)
- Thus physiological concentrations of zinc inhibit the release of histamine from human basophils and lung mast cells, presumably by blocking Ca2+ uptake induced by anti-IgE activation. (nih.gov)
Granules2
- [ 8 ] As such, tryptase is specific to mast cell granules and can provide information about mast cell number, distribution, and activation depending on the clinical context. (medscape.com)
- The secretion of histamine from mast cell and basophil granules by exocytosis. (bvsalud.org)
Stimulation1
- Adherence reaction mediated through the CR receptors on phagocytes lead to stimulation of phagocytosis, activation of metabolism and secretory function and movement of phagocytes into the inflammatory site. (dadamo.com)
Allergy1
- While BATs are better than SPTs and peanut-specific IgE tests at distinguishing peanut allergy from peanut sensitization, basophil testing requires fresh blood and cannot be performed on stored samples. (foodallergy.org)
Monocytes1
- FcεRI is expressed on mast cells, basophils, and at lower levels, on dendritic cells and monocytes. (bdbiosciences.com)
Vitro1
- Human IL-5 is a potent eosinophil differentiation and activation factor in vivo and in vitro . (rndsystems.com)
Assays1
- It could be used with other immune functional assays - tests that doctors use to assess particular therapeutic treatments at the cellular level - that require other white blood cell types, not just basophils. (stanford.edu)
Mast cell activation2
- The concept that disease rooted principally in chronic aberrant constitutive and reactive activation of mast cells (MCs), without the gross MC neoplasia in mastocytosis, first emerged in the 1980s, but only in the last decade has recognition of "mast cell activation syndrome" (MCAS) grown significantly. (degruyter.com)
- Subsequent literature regarding this newly recognized (but of course not truly new) "mast cell activation syndrome" (MCAS) included case reports as well as formal studies (mostly relatively small scale), reviews, and various proposals for formal diagnostic criteria. (degruyter.com)
Differentiation1
- Additionally, it has also been reported that IL-5 can stimulate the proliferation and/or differentiation of basophils and B cells. (rndsystems.com)
Angioedema1
- Urticaria also may be accompanied by angioedema, which results from mast cell and basophil activation. (merckmanuals.com)
Antibody1
- We use an antibody cocktail, which targets non-basophils. (stanford.edu)
Clinically1
- Using their basophils, 95% or more of patients with some clinically significant allergies can be identified. (stanford.edu)
Inhibition3
- PKC inhibition reversed the activation of proton channels, and the proton channel response to anti-IgE or PMA persisted in Ca 2+ -free solutions. (johnshopkins.edu)
- title{Protein Tyrosine Phosphatase Inhibition Mimics Fc$\varepsilon$RI-induced Anergy and Reveals the Immunomodulatory Role of Hydrogen Peroxide as a Signaling Molecule in Human Basophils\thanksref{grant}} %Really struggling with a title that captures this paper -- probably a bad sign! (openwetware.org)
- Preincubation (5 min, 37 degrees C) of human basophils and lung mast cells with zinc chloride (10(-6)-3 X 10(-5) M) caused dose-related inhibition of histamine and peptide leukotriene C4 (LTC4) release induced by anti-IgE. (nih.gov)
Cells11
- This is why basophils are considered with mast cells to be the key cells in allergic diseases. (wikipedia.org)
- But basophils are exceedingly rare in a typical vial of blood, composing 1% or less of all white blood cells. (stanford.edu)
- In order to advance the science of food allergies - and to learn more about these elusive cells - engineers and clinicians at Stanford University have focused their attention on a way to isolate basophils. (stanford.edu)
- Graphic by Kateryna Kon , Shutterstock: Illustration of a basophil among red blood cells. (stanford.edu)
- Then, to isolate the basophils, the researchers pull cells through a device that applies a custom-designed magnetic field that attracts and immobilizes everything that's not a basophil. (stanford.edu)
- The cells flow through a progressively stronger magnetic field to ensure the resulting fluid contains pure basophils. (stanford.edu)
- Flow cytometry demonstrated a significant expansion of CD4+ T cell population and dramatically increased CD69+ cells among CD4+ T cells in MCD, suggesting coincident activation of T cells with onset of the disease. (scienceopen.com)
- Our results reveal an association between onset of MCD and activation of Th2 cells. (scienceopen.com)
- Both IgE and non-IgE activation of mast cells and basophils ignites a cascade that results in the release and production of several inflammatory and vasoactive substances. (medscape.com)
- The IgE resides on the mast cells and basophils. (medscape.com)
- Tryptase is a trypsin-like proteinase that is found most abundantly in mast cells and basophils, with the former containing almost 300 times more tryptase. (medscape.com)
Inhibits2
- it inhibits transcription for genes that encode IL-3, IL-4, IL-5, GM-CSF, and TNF-alpha (all involved in early T-cell activation). (medscape.com)
- It leads to decreased expression of IL-3, IL-4, IL-5, and TNFα, and it inhibits mast cell and neutrophil activation. (medscape.com)
Mechanism2
- To demonstrate a specific IgE mechanism of basophil activation, BAT with wortmannin pre-treatment was performed in four LAR patients. (biomedcentral.com)
- Different fragments, released from individual components during complement activation, operate by a non-cytolytic mechanism through specific receptors present on various cell types. (dadamo.com)
Antibodies1
- T-cell immunity is cellular and involves the activation of phagocytes and B-cell immunity uses antibodies to fight infection. (loinc.org)
Specific1
- As such, determinations of tryptase levels are contingent on both the size and activation status of an individual's mast cell population but is not informative of the specific contribution of either of these factors. (medscape.com)
Results2
- In addition, a significant fraction of individuals have non-reactive basophils that give inconclusive BAT results. (foodallergy.org)
- Activation of the complement cascade, with the formation of the effector MAC unit, results in cytotoxic and cytolytic reactions. (dadamo.com)
Expression1
Significant1
- However, at a concentration of 1000 ng/mL, no significant differences in basophil activation were observed between reactors and nonreactors. (jiaci.org)