• We evaluated FOXP1 activity by an in vitro luciferase model and assessed protein stability in vitro by western blotting. (bmj.com)
  • DUSP3/VHR, related to the vaccinia virus phosphatase VH1, is initially found to be a phosphatase that dephosphorylates multiple protein tyrosine kinases (PTKs) in vitro [ 21 ]. (biomedcentral.com)
  • Nck-associated protein 1 (NAP1/NCKAP1), a protein that associates with the Src homology 3 (SH3) domain of NCK protein both in vitro and in intact cells, was found to localize along the lamellipodia and to mediate contact-dependent cell migration [ 4 ]. (biomedcentral.com)
  • Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
  • and cross-linking agents confer this coherence on intracellular F-actin ( Matsudaira, 1994 ). (rupress.org)
  • Intracellular L. monocytogenes replicate in the cytoplasm of host cells and induce the polymerization of host actin filaments at the bacterial surface. (silverchair.com)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Upon i.m. injection of DNA vectors expressing Nef mut -derivatives, nanovesicles containing antigens fused with Nef mut are released by muscle cells, can freely circulate into the body, and can be internalized by antigen-presenting cells (APCs). (nature.com)
  • QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
  • The pQE vectors (see table and figure pQE Vectors. Numbered elements listed in table. "> pQE Vectors ) enable placement of the 6xHis tag at either the N- or C-terminus of the recombinant protein. (qiagen.com)
  • To define the molecular basis of the IL-6-induced mitogenic response in myeloma cells, we applied STAR (subtractive transcriptional amplification of mRNA), a new differential expression analysis technology, to isolate mRNAs preferentially expressed in IL-6-treated versus untreated cultures of the factor-responsive myeloma cell line U266. (elsevierpure.com)
  • The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
  • Most of the important molecular processes in the cell, such as DNA replication, are performed by large molecular network of protein-protein interactions. (researchsquare.com)
  • As a result, there are various molecular forms with distinct sizes for this protein, while the precise nature of each form remains elusive. (bvsalud.org)
  • The identification of new genes/proteins involved in breast cancer (BC) occurrence is widely used to discover novel biomarkers and understand the molecular mechanisms of BC initiation and progression. (bvsalud.org)
  • Together these proteins form a molecular switch that can be turned on and off. (elifesciences.org)
  • 1. Identification and characterization of Arabidopsis rat mutants and molecular cloning of RAT genes (Gelvin, Citovsky, Hohn, and Ziemienowicz laboratories, with help from undergraduate students at Tuskegee University). (purdue.edu)
  • Furthermore, to the best of our knowledge, there are only a few reports on the role of molecular motor and TCA cycle-related proteins in the salivary glands of ticks. (biomedcentral.com)
  • Using bioinformatics methods to analyze the dynamic changes of a large number of proteins, we found that molecular motor and TCA cycle-related proteins play an important role in the physiological changes of the salivary glands. (biomedcentral.com)
  • By analyzing these changes, some proteins, such as molecular motor and TCA cycle-related proteins, were screened and RNAi carried out. (biomedcentral.com)
  • This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. (cancerindex.org)
  • The production of these constructs induced the formation of high molecular aggregates with a potential impact on the efficacy and safety of the protein. (bmj.com)
  • The molecular cloning of GCSF gene (Nagata et al. (allindianpatents.com)
  • Filamin A (FLNa) can effect orthogonal branching of F-actin and bind many cellular constituents. (rupress.org)
  • The dynamic remodeling of actin filaments (F-actin) concentrated at the cell periphery is responsible for cell translocation, for cell shape changes, and for cellular resistance to potentially disruptive mechanical stresses. (rupress.org)
  • The regulatory mechanism of lncSNHG5 was investigated by RNA sequencing, fluorescent in situ hybridization, cellular fractionation assay, mass spectrometry, RNA pull-down, RNA immunoprecipitation, gene-specific m6A assay, chromatin immunoprecipitation, dual luciferase reporter assay and actinomycin D treatment in CAFs and NFs. (thno.org)
  • 2006). ED also dimerizes and binds several cellular proteins (Aramini et al. (datexis.com)
  • This is driven by cellular proteins which tether the lentiviral preintegration complexes to specific sites on the host chromosomes. (biomedcentral.com)
  • Mechanistic studies further reveal that the binding of NAP1 to the cellular chaperone heat shock protein 90 (HSP90) is required for its protein stabilization, and NAP1 plays an essential role in HSP90-mediated invasion and metastasis by provoking MMP9 activation and the epithelial-to-mesenchymal transition in NSCLC cells. (biomedcentral.com)
  • Northern blot analysis documented that two transcription factor genes chosen for further study, c-myc promoter-binding protein (MBP-1) and X-box binding protein 1 (XBP-1), were up-regulated in U266 cells about 3-fold relative to the cell cycle-dependent β-actin gene 12 h after IL-6 treatment. (elsevierpure.com)
  • Lysates of (a) BSC-1 or (b to d) SK-MEL-2 cells were immunoblotted against CLTA, DNM2, RFP, GFP, or actin. (accessibletech4all.org)
  • To define the localization of WDFY2 in the endocytic pathway, we transiently transfected hTERT-RPE1 cells with green flourescent protein (GFP)-WDFY2 and performed structured illumination microscopy (SIM) together with APPL1 and EEA1 visualized with antibodies (Fig. 1a ). (nature.com)
  • Thus, existing techniques and current knowledge have not used nor addressed the interactions of GEF-H1 with other proteins as a means by which cell proliferation can be controlled or the detection and treatment of cancerous, tumorigenic cells and tissues be developed. (justia.com)
  • There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. (qiagen.com)
  • The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
  • Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)
  • Recently it has been shown that HPV16 E5 may co-precipitate with the heavy chain of HLA-I in cells over-expressing the E5 protein [ 21 ]. (biomedcentral.com)
  • FGF-source and recipient ASP cells extend cytonemes that present FGF and FGFR on their surfaces and reciprocally recognize each other over distance by contacting through CAM-like FGF-FGFR binding. (biorxiv.org)
  • Contact-mediated FGF-FGFR binding induces bidirectional signaling, which, in turn, promotes ASP and source cells to polarize cytonemes toward each other and reinforce signaling contacts. (biorxiv.org)
  • These conserved families of secreted morphogens/signals, such as Fibroblast Growth Factor (FGF), Hedgehog (Hh), Wingless (Wg)/Wnt, Epidermal Growth Factor (EGF), and Decapentaplegic (Dpp - a BMP homolog), act away from their sources and, upon binding to receptors, activate gene regulatory pathways to induce functions in recipient cells 1 , 2 . (biorxiv.org)
  • The 70 or so members of the Rab subfamily of proteins perform a wide range of important tasks inside cells. (elifesciences.org)
  • The K V 10.1-specific scFv62 antibody -TRAIL fusion protein was expressed in CHO-K1 cells, purified by chromatography and tested for biological activity. (biomedcentral.com)
  • In co-cultures with K V 10.1-positive cancer cells the fusion protein also induced apoptosis in bystander K V 10.1-negative cancer cells, while normal prostate epithelial cells were not affected when present as bystander. (biomedcentral.com)
  • TRAIL is normally present as a membrane protein (memTRAIL) on immune effector cells, like natural killer cells. (biomedcentral.com)
  • In mammalian cells, active and silent rDNA promoters are characterized by specific histone modifications, DNA methylation, and positioning of the promoter-bound nucleosome (reviewed in reference 22 ). (asm.org)
  • Chromatin binding assays in yeast and mammalian cells demonstrated that these IN mutants were impaired for the ability to bind chromatin. (biomedcentral.com)
  • We previously showed that the fusion of the serine protease granzyme B (GrB), the effector molecule or T and B cells, to a binding domain allows the controlled and effective delivery of the cytotoxic payload into the target cell. (bmj.com)
  • Tight junctions (TJ) are multi-protein complexes that hold epithelial cells together and form structural and functional barriers for maintaining proper biological activities. (biomedcentral.com)
  • Compared to those of the DUSP3-expressing cells, we found the expression and distribution of ZO-1, a TJ-anchoring molecule, were abnormal in DUSP3-deficient cells. (biomedcentral.com)
  • TJs, tightly fused regions between the outer membrane of adjacent cells, are formed by integral transmembrane proteins such as OCLN and claudins, anchoring proteins such as zonula occludens (ZO)1-3, and other TJ-associated/regulatory proteins [ 4 , 5 ]. (biomedcentral.com)
  • however, this region is dispensable for actin-based motility in cells. (silverchair.com)
  • Polarized polymerization of actin propels the bacteria through the cytoplasm and into pseudopod-like structures that are engulfed by neighboring cells. (silverchair.com)
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (thermofisher.com)
  • Our previous studies further demonstrated that NAP1 is required for the protein stability of WASF3 in breast cancer cells, implicating that NAP1 is a critical regulator in favor of breast cancer metastasis [ 5 ]. (biomedcentral.com)
  • Only anti-EspA is found in LEE proteins is triggered by close contact with host cells. (cdc.gov)
  • 16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • A similar coiled-coil domain is present in several putative human proteins and in the mitotic phosphoprotein 43 ( MP43 ) of X. laevis. (xenbase.org)
  • Nck-associated protein 1 (NAP1/NCKAP1) is highly expressed in primary non-small-cell lung cancer (NSCLC) when compared with adjacent normal lung tissues, and its expression levels are strongly associated with the histologic tumor grade, metastasis and poor survival rate of NSCLC patients. (biomedcentral.com)
  • Dual specificity phosphatase 3 (DUSP3), a suppressor of multiple protein tyrosine (Tyr) kinases, is decreased in lung cancer tissues. (biomedcentral.com)
  • Diverse KRAB-ZFPs recognize specific DNA sequences with the majority targeting endogenous retroviruses, ensuring their repression. (elifesciences.org)
  • It contains a lipid-binding FYVE domain and seven WD40 repeats which can form a β-propeller. (nature.com)
  • This de novo assembly of a red clover transcriptome from leaf material of droughted and non-droughted plants provides a rich source for gene identification, single nucleotide polymorphisms (SNP) and short sequence repeats (SSR). (biomedcentral.com)
  • FLNa dimeric subunits have N-terminal spectrin family F-actin binding domains (ABDs) and an elongated flexible segment of 24 immunoglobulin (Ig) repeats. (rupress.org)
  • We find that Ig repeats 9-15 contain an F-actin-binding domain necessary for high avidity F-actin binding. (rupress.org)
  • Ig repeats 16-24, where most FLNa-binding partners interact, do not bind F-actin, and thus F-actin does not compete with Ig repeat 23 ligand, FilGAP. (rupress.org)
  • Ig repeats 16-24 have a compact structure that suggests their unfolding may accommodate pre-stress-mediated stiffening of F-actin networks, partner binding, mechanosensing, and mechanoprotection properties of FLNa. (rupress.org)
  • The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
  • It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. (wikipedia.org)
  • In addition to direct interaction, NS1 has been shown to inhibit tripartite motif-containing protein 25 (TRIM25)-mediated RIG-I ubiquitination, which suppresses the RIG-I signaling pathway (Gack et al. (datexis.com)
  • The selected RNA aptamers bind to NS1 specifically and inhibit the interaction between NS1 and TRIM25. (datexis.com)
  • Overall, this study demonstrates that three mutations located in the C-terminal region of the catalytic core domain of HIV-1 IN inhibit the IN-induced lethal phenotype in yeast by inhibiting the binding of IN to the host chromatin. (biomedcentral.com)
  • Efimov VP and Morris NR (2000), The LIS1-related NUDF protein of Aspergillus ni. (xenbase.org)
  • Because nucleic acid aptamers have comparable binding affinities with corresponding antibodies, they are regarded as potential candidates for the development of therapeutic agents or diagnostic probes (Brody and Gold, 2000). (datexis.com)
  • However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)
  • These transcript tags were annotated with gene ontology (GO) terms. (biomedcentral.com)
  • One of the most striking results from the expression analysis was that the drought sensitive plants were characterised by having approximately twice the number of differentially expressed transcript tags than the tolerant plants after drought. (biomedcentral.com)
  • Finally, we showed that miR-486-5p suppressed NEK2 by directly binding to its transcript 3'UTR. (oncotarget.com)
  • The membrane-bound matrix metalloproteinase MT1-MMP (MMP14) is internalized by endocytosis and recycled in endosomal compartments. (nature.com)
  • Here, we have studied the function of WDFY2, a cytosolic protein that has been described to reside on endocytic vesicles close to the plasma membrane 6 . (nature.com)
  • E5 impairs ligand-mediated apoptosis by reducing the amount of surface CD95 proteins or inhibiting the formation of the DISC complex [ 12 ], and affects the normal functioning of a number of membrane associated proteins, probably by modifying the composition and the interactions in the cell membranes [ 13 ]. (biomedcentral.com)
  • Subsequent un-anchoring of FGFR-bound-FGF from the source cell membrane dissociates cytoneme contacts and delivers FGF target-specifically to ASP cytonemes for paracrine functions. (biorxiv.org)
  • The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)
  • The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. (cancerindex.org)
  • AURK significantly promotes activation of IκBα at serine 32, but not serine 36 residues, unlike IκB kinase (IKK) family proteins activate both IκBα serine residues. (researchsquare.com)
  • This was in contrast to deglycosylation by Endo H, which resulted in a clear size-shift, even though N-linked GlcNAc residues remained on the protein. (bvsalud.org)
  • Strains were transformed with nudE variants in pAid vector and grown at 43°C. Numbers refer to amino acid residues of NUDE protein expressed by the constructs (see Fig. 3 for detailed amino acid sequences). (xenbase.org)
  • The experiments showed that different amino acids in the active site of the Rab protein are involved when the GEFs mediate the release of the GDP during the activation process. (elifesciences.org)
  • The low fre- and EPEC, the proteins that mediate this attachment are quency of antibodies to Stx1 (in 12% of Mexican and in encoded in a chromosomal pathogenicity island called the 22% of U.S. samples) suggests that the rare appearance of "locus of enterocyte effacement" (LEE) (3). (cdc.gov)
  • HUS complicates brane protein (9,10) and triggers the host cytoskeletal approximately 5% to 8% of infections caused by E. coli events that lead to attaching/effacing lesion formation. (cdc.gov)
  • The Listeria monocytogenes ActA protein mediates actin-based motility by recruiting and stimulating the Arp2/3 complex. (silverchair.com)
  • The ability of VASP to contribute to actin filament nucleation and to regulate actin filament architecture highlights the central role of VASP in actin-based motility. (silverchair.com)
  • Our study revealed that nutlin-3a is a PARP1 suppressor that induces PARP1 proteasomal degradation by binding to MDM2 and promoting autoPARylation of PARP1. (oncotarget.com)
  • The indicated mutants (all are conditional, temperature sensitive) were transformed with either the empty vector pAid or pAid clones bearing nudE and nudF6 genes (plasmids recovered in the multicopy suppressor screen) and grown at 32°C. Transformants were gridded on YAG plates with or without KCl and incubated at 43°C or 32°C for 2 d. (xenbase.org)
  • Serine/threonine-protein kinase MRCK alpha is an enzyme that in humans is encoded by the CDC42BPA gene. (wikipedia.org)
  • The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. (wikipedia.org)
  • Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). (wikipedia.org)
  • This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. (wikipedia.org)
  • Methods: Previously we observed that aurora kinase (AURK) binds IκBα in the cell. (researchsquare.com)
  • In addition, we show that the M1 mutant is only able to marginally down-regulate HLA-I surface expression compared to the wild-type protein. (biomedcentral.com)
  • The expression levels of a large number of proteins during the development and degeneration of salivary glands change, which regulate the biological functions of the salivary glands. (biomedcentral.com)
  • The Rab subfamily of proteins is part of the large Ras superfamily, and all members of this superfamily are activated and inactivated in a similar way, with the binding and unbinding of GDP and GTP taking place at a structure called the G-domain. (elifesciences.org)
  • In vertebrates this subfamily contains four proteins: TIF1α/TRIM24, TIF1β/TRIM28, TIF1γ/TRIM33, and TIF1δ/TRIM66, while only one protein, Bonus (Bon), is present in Drosophila , making it an attractive model to understand the conserved functions of TIF1 proteins. (elifesciences.org)
  • miRNAs induce gene degradation and suppression of translation, or both, mainly by imperfect binding to miRNA recognition elements (MREs) within the 3′-untranslated region (UTR) of target mRNAs ( 1 ). (frontiersin.org)
  • In doing so, this GEF-facilitated exchange of GDP for GTP is associated with structural changes in the GTP-binding protein which influence the degree to which the GTP-binding protein can interact with other molecules. (justia.com)
  • When GTP is bound, for instance, Ras proteins can interact with effectors and other molecules to affect cell proliferation, differentiation and apoptosis. (justia.com)
  • The fact that NUDF and LIS1 interact with the same protein domain strengthens the notion that these two proteins are functionally related. (xenbase.org)
  • Additionally, we determined that while these IN mutants failed to interact with LEDGF/p75, they retained the ability to bind Integrase interactor 1. (biomedcentral.com)
  • In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
  • In another embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is less than 25 amino acids in length. (justia.com)
  • In a more preferred embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is 18 amino acids long. (justia.com)
  • For example, the amino acid glutamine is involved when the Listeria GEF and one of the human GEFs activate the protein, whereas a different amino acid-aspartate-is involved when one of the other human GEFs is responsible for the activation. (elifesciences.org)
  • NS1 is comprised of two functional domains, N-terminal RNA-binding domain (RBD) and C-terminal effector domain (ED). RBD, amino acids 1-73 of NS1, forms a six-helical homodimer, and its major role is double-stranded RNA (dsRNA) binding, thereby blocking IFN induction on infection (Pichlmair et al. (datexis.com)
  • The hOAT3 cDNA consisted of 2179 base pairs that encoded a 543-amino-acid residue protein with 12 putative transmembrane domains. (aspetjournals.org)
  • The deduced amino acid sequence of hOAT3 showed 36 to 51% identity to those of other members of the OAT family. (aspetjournals.org)
  • The present invention relates to a chimeric protein of recombinant human granulocyte colony stimulating factor comprising of hG-CSF fused to an affinity fusion tag at its amino terminus. (allindianpatents.com)
  • IκBα inhibits the NF-κB by keeping them in a state isolated from the inactive cytoplasmic mask and the nuclear localization signal of NF-κB proteins. (researchsquare.com)
  • To date, we have sequenced junctions from 23 rat mutants. (purdue.edu)
  • Many of these junctions lie in or near "unknown" or "hypothetical" proteins. (purdue.edu)
  • 2 − 6 These nuclease-resistant polymers persist in live organisms for days and are typically designed to recognize 25-base sequences that span intron-exon junctions or translational start sites. (cdc.gov)
  • 1986, Science 232, 61-64) led to the production of recombinant protein and large scale manufacturing of the product. (allindianpatents.com)
  • Background Mutations in forkhead box protein P1 ( FOXP1 ) cause intellectual disability (ID) and specific language impairment (SLI), with or without autistic features (MIM: 613670). (bmj.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • We need to acquire more knowledge of various protein-protein interactions in order to understand biological phenomena, including diseases, and to provide the basis for new therapeutic approaches. (researchsquare.com)
  • Protein-protein interactions were determined by immunoprecipitation. (biomedcentral.com)
  • CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. (thermofisher.com)
  • Injury-induced cytochrome c -specific cleavage of caspase-9 followed by activation of caspase-3 in mature brain correlated with marked increases in Apaf-1 and caspase-3 mRNA and protein expression. (jneurosci.org)
  • lncSNHG5 enhanced ZNF281 mRNA stability by binding with the m6A reader IGF2BP2. (thno.org)
  • Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
  • Among the known genes uncovered in the screen were those encoding proteins that function in cell division, cell signalling and gene/protein expression. (elsevierpure.com)
  • This clone was engineered to express the complete ORF with an expression tag. (origene.com)
  • Each fiber type-specific sample is then used to quantify the expression of various target proteins using western blotting techniques. (bvsalud.org)
  • The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
  • Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
  • Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
  • The human papillomavirus type 16 E5 protein (HPV16 E5) down-regulates surface expression of HLA-I molecules. (biomedcentral.com)
  • The results showed that the expression of different types of proteins change in different characteristics in salivary glands during the unfed to engorged process of female ticks. (biomedcentral.com)
  • Corresponding expression changes of these proteins at different developmental stages of female ticks are very important to ensure the orderly development of the organ. (biomedcentral.com)
  • Western blotting confirmed the expression of DRAM2, p53, and the other involved proteins. (cancerindex.org)
  • DRAM2 overexpression also increased proteins CDK4, CyclinD3, and decreased p27 expression, all of which are cell cycle-related factors. (cancerindex.org)
  • Moreover since high levels of biologically active protein expression are achieved in E. coli, it is the host of preference for producing G-CSF. (allindianpatents.com)
  • One way to produce recombinant human G-CSF in soluble form is to produce chimeric protein formed by expression of a hybrid gene made by splicing together two gene sequences, In this approach, ideally GCSF would be operationaffy finked to an affinity tag so as to give chimeric protein with better solubility. (allindianpatents.com)
  • 2008), which is responsible for sequence variations of different virus strains. (datexis.com)
  • All vaccine preparations were conceived to elicit anti-Spike protein immune responses, and their effectiveness relies on the generation of neutralizing antibodies. (nature.com)
  • Single-chain antibodies (scFv) take an important role in the field, because they are less immunogenic than whole antibodies, their smaller size allows faster and deeper penetration into solid tumors, and are by definition recombinant proteins, therefore easier to produce and modify. (biomedcentral.com)
  • Accordingly, the present invention envisions an isolated nucleic acid encoding the GEF-H1S polypeptide of SEQ ID NO:2, as well as an isolated nucleic acid comprising the sequence of SEQ ID NO:1. (justia.com)
  • Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)
  • We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)
  • Protein levels were assessed by Western blotting and immunohistochemistry. (biomedcentral.com)
  • Physique S4 Endocytic protein lifetime distribution of genome-edited cell lines. (accessibletech4all.org)
  • Lifetime distribution for endocytic proteins was determined by the elapsed time between the appearance and disappearance of fluorescent structures present in the time series (6 min) from TIRF microscopy time-lapse videos of (a) BSC-1, (b) SK-MEL-2 CLTA-RFP and (c) SK-MEL-2 DNM2-GFP cell lines. (accessibletech4all.org)
  • The traditional triad in the medical diagnosis of multiple myeloma may be the recognition of M proteins in the serum or urine (Bence-Jones proteins), higher than 10% plasmacytosis in the bone tissue marrow, and the current presence of osteolytic lesions by radiography. (siamtech.net)
  • How signaling proteins generate a multitude of information to organize tissue patterns is critical to understanding morphogenesis. (biorxiv.org)
  • This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
  • Our insights demonstrate the importance and functional regulation of the HSP90-NAP1 protein complex in cancer metastatic signaling, which spur new avenues to target this interaction as a novel approach to block NSCLC metastasis. (biomedcentral.com)
  • Background: The phosphorylation of NF-kappaB inhibitor alpha (IκBα) protein is pivotal to the regulation of NF-κB transcription factor activity in the cell. (researchsquare.com)
  • Multiple alternatively spliced variants, encoding the same protein, have been identified. (cancerindex.org)
  • Of the 22 clones that produced legible sequence, 19 perfectly matched WT DNM2 near the ZFN cleavage and donor insertion sites, and three matched the expected sequence of the GFP donor (note that the transgenic allele is usually a less efficient substrate both for cloning and for amplification). (accessibletech4all.org)
  • Until early 1990s, renal organic anion transport was thought to be carried out by a few carrier proteins that showed wide substrate specificity. (aspetjournals.org)
  • From the resulting collection of STAR clones, sequence information was obtained for a total of 72 distinct transcripts. (elsevierpure.com)
  • IN is a 32 kDa protein with three distinct structural domains, the N-terminal zinc-binding domain, the central catalytic core domain and the C-terminal domain. (biomedcentral.com)
  • A) Homology between A. nidulans NUDE protein and N. crassa RO11 protein. (xenbase.org)
  • Protein synthesis is effectively blocked in the presence of high levels of lac repressor and the stability of cytotoxic constructs is enhanced. (qiagen.com)
  • Here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (VASP) recruitment by ActA can bypass defects in actin monomer-binding. (silverchair.com)
  • Guanine nucleotide exchange factors ("GEFs") stimulate the dissociation of the GTP hydrolytic product, GDP, from small GTP-binding proteins, to promote the binding of a new GTP molecule. (justia.com)
  • A Rab protein is always bound to another molecule, which determines whether it is inactive or active. (elifesciences.org)
  • Binding to a molecule called GDP makes the Rab protein inactive, while binding to GTP makes it active. (elifesciences.org)
  • We developed a CD8 + T-cell-based vaccine platform based on intramuscular (i.m.) injection of a DNA vector coding for antigens of interest fused at the C-terminus of a biologically inactive Human Immunodeficiency Virus (HIV)-Type 1 Nef protein (Nef mut ) having an unusually high efficiency of incorporation into EVs. (nature.com)
  • Green shading indicates the GFP coding sequence provided by the donor.Physique S2 Western immunoblot analysis of genome-edited cell lines. (accessibletech4all.org)
  • Top panel: The number of genotyped clones is usually given for each target in each cell type, along with the number (percentage) of correctly tagged clones having at least one (but less than all) allele tagged, or all alleles tagged. (accessibletech4all.org)
  • We previously analyzed the interacting partners of JTBhigh protein extracted from transfected MCF7 BC cell line using SDS-PAGE complemented with in-solution digestion, respectively. (bvsalud.org)
  • Upon upwards migration towards more superficial layers and concomitant differentiation of the infected keratinocyte, the late genes of the virus are expressed leading to the formation of viral particles and their release upon cell death. (biomedcentral.com)
  • Results GrB(C210A)-Fc-IT4 protein exhibited high affinity and selective cytotoxicity within the nanomolar range when tested against a panel of Fn14-positive human cancer cell lines. (bmj.com)
  • However G-CSF in E. coli is expressed as inclusion bodies in the form of insoluble protein, which necessitates the additional steps for the isolation and purification of GCSF. (allindianpatents.com)
  • The LIS1 -related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein. (xenbase.org)
  • The highly conserved NH(2)-terminal coiled-coil domain of the NUDE protein suffices for protein function when overexpressed. (xenbase.org)
  • TRIM/RBCC is an ancient protein family characterized by the presence of an N-terminal RING finger domain closely followed by one or two B-boxes and a coiled coil domain. (elifesciences.org)
  • We generated a library of FLNa fragments to examine their F-actin binding to define the structural properties of FLNa that enable its various functions. (rupress.org)
  • Our study revealed that DUSP3 is an important TJ regulatory protein and its decrease may be involved in progression of epithelial cancers. (biomedcentral.com)
  • States, the predominant EHEC serotype associated with brane, where it binds to intimin, a bacterial outer mem- serious disease is E. coli O157:H7. (cdc.gov)