• The bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). (biomedcentral.com)
  • In vitro, MPL515/630 but not MPL515/625 or MPL515/625/630 retained the ability to induce TPO-independent proliferation and increase colony-forming unit megakaryocytes (CFU-Mk). (biomedcentral.com)
  • We hypothesized that changes in morphology may indicate changes in cell wall metabolism and identified an aceE gene mutant ( aceE -mut) which presented a deficient colony morphology on 7H10 agar by screening transposon mutagenesis in Mycolicibacterium smegmatis , basonym Mycobacterium smegmatis ( M. smegmatis ). (biomedcentral.com)
  • We observed that the colony morphology of aceE -mut was quite different, smaller and smoother on the solid culture medium than the wild-type (WT) strain during the transposon library screening of M. smegmatis . (biomedcentral.com)
  • Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage. (biomedcentral.com)
  • This mutant had obvious differences in colony morphology (smaller plaque, edge smooth and round) and defects in biofilm formation in contrast with the wild-type (WT) strains. (biomedcentral.com)
  • UW Δ mlaF has a distinct colony morphology. (elifesciences.org)
  • Enzyme assays confirm that PTC-847 and PTC-672 inhibit Ng RNR and reveal that allosteric effector dATP potentiates the inhibitory effect. (elifesciences.org)
  • Other TB diagnostic methods include blood cultures for extrapulmonary TB (ETB) and serologic tests by Enzyme-linked ImmunoSorbent Assay (ELISA), which do not present sufficient sensitivity and specificity to be useful and determination of adenosine deaminase. (scielo.org.mx)
  • CA-125 levels were quantified by chemiluminescent enzyme immuno-assay. (go.jp)
  • This bacterium causes chronic gastritis and duodenal ulcers, and produces the enzyme urease, which neutralizes gastric acid locally, enabling colonization of the stomach. (biomedcentral.com)
  • Assay specificity was evaluated using three E. coli K12 strains (ATCC W3110, MG1655 & DH1), 24 non-K12 E. coli and 23 bacterial genera. (who.int)
  • Compared to culture-based DS tests, the MTBDR( plus ) line probe assay displays a pooled sensitivity of 0.98 and a pooled specificity of 0.99 for detecting rifampin resistance in isolates or directly from clinical specimens (10-12). (cdc.gov)
  • A meta-analysis of the performance of the Hain MTBDR (plus) assay for detecting isoniazid resistance revealed a pooled sensitivity of 0.85 (95%CI 0.77- 0.90) and a pooled specificity of 0.99 (95%CI 0.98-1.00) (11,12). (cdc.gov)
  • Investigators assayed soluble immune factors and the absolute abundance of bacterial taxa using multiplex ELISA and quantitative polymerase chain reaction (PCR). (naturalmedicinejournal.com)
  • Our study also suggests that MPL cytosolic phosphorylated Y625 and flanking amino acids could become targets for pharmacologic inhibition in MPNs. (biomedcentral.com)
  • Global responses in both bacteria included competition for nitrogen sources, amino acids and increased tRNA levels. (biomedcentral.com)
  • Notably, in contrast with the WT, which aggregates and forms biofilm, the aceE -mut lost its ability of growing aggregately and biofilm formation, which are two very important features of mycobacteria. (biomedcentral.com)
  • The biofilm detection limit was 10(3) colony-forming units (CFU) E. coli K12 mL(-1), but required a modified protocol, which included a bio-blocker Pseudomonas aeruginosa with ethylenediaminetetraacetic acid buffered to pH 5 prior to cell lysis/DNA extraction. (who.int)
  • Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against B. subtilis and S. aureus, respectively. (scielo.br)
  • The heterotrophic bacteria count and GNNR identification were performed on water samples collected from 25 dental units (air/water syringe and reservoir). (bvsalud.org)
  • The quantity of isolated bacteria from the reservoir was lower than from the air/water syringe in 88% of the dental units. (bvsalud.org)
  • Yet, there is no standard procedure and the water used in dental units during dental treatment still shows high amounts of heterotrophic bacteria 25 . (bvsalud.org)
  • Absorbance assay, crystal violet staining and adherence assay were used to detect the antifungal activity of flavopiridol. (researchsquare.com)
  • The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
  • In order to eliminate 4-MU from the cytosol, the xenobiotic-detoxification machinery of MSC covalently attaches glucuronic acid (GlcUA) to 4-MU, thereby depriving HAS from UDP-GlcUA thus ceasing HA production. (europa.eu)
  • The carboxyl group of mycolic acids is vertically covalently linked to the hydroxyl group of arabino-galactan by ester bond, arabino-galactan is linked to the peptidoglycan layer by phospholipid bond, whereas other glycolipids and free lipids are regularly distributed in the thicker layer of mycolic acids [ 3 , 4 , 5 ]. (biomedcentral.com)
  • In the California Microbial Diseases Laboratory, molecular beacon assays were designed to detect mutations in the rpoB gene directly from clinical specimens and from cultures. (cdc.gov)
  • The analysis of mycolic acid (MA) using LC-MS indicated deficiency of alpha-MA and epoxy-MA in aceE -mut strain whereas complementation of the aceE -mut with a wild-type aceE gene restored the composition of MA. (biomedcentral.com)
  • A quantitative polymerase chain reaction assay (115 bp amplicon) specific to Escherichia coli K12 with an ABI(TM) internal control was developed based on sequence data encoding the rfb gene cluster. (who.int)
  • This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)
  • For hybridization assays such as the INNO-LiPA® Rif.TB (Innogenetics) and GenoType® MTBDR( plus ) (Hain LifeScience GmbH) line-probe assays, the region of a gene associated with resistance is PCR amplified, and the labeled PCR products hybridized to oligonucleotide probes immobilized on a nitrocellulose strip. (cdc.gov)
  • Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance ( mec A) gene was detected by conventional PCR assay. (who.int)
  • KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)
  • According to the World Health Organization (WHO), TB is responsible for about 1.6 million TB deaths and 10 million (5.8 million men, 3.2 million women, and 1.0 million children) new cases have been detected in 2017 and more than a third of the world population is hosting Mycobacterium tuberculosis , the causative pathogen of TB, in its latent form ( 1 , 2 ). (frontiersin.org)
  • Gram negative isolates showed high resistance rate of 73.1% to ampicillin and 65.4% to amoxicillin-clavulanic acid while Gram-positive isolates showed high resistant rate of 94.1% to penicillin. (who.int)
  • Tuberculosis is a highly infectious disease that is spread from person to person by infected aerosols emitted by patients with respiratory forms of the disease. (biomedcentral.com)
  • Mycobacterium tuberculosis , the causative agent is spread from person to person via infected aerosols created by patients with respiratory forms of the disease. (biomedcentral.com)
  • Water samples were assayed for potential respiratory viruses (adenoviruses and enteroviruses), but none were detected. (cdc.gov)
  • The key advantage of the molecular tests is that they can provide results within 24 to 48 hours, because they take advantage of the speed of nucleic acid amplification. (cdc.gov)
  • Genetic or molecular tests for detecting mutations are, in general, variations of nucleic acid amplification (NAA) tests. (cdc.gov)
  • With the unique combination of a targeted menu of molecular POC assays, the cobas ® Liat ® System, and the cobas ® infinity POC solution, Roche offers the total point-of-care PCR solution, empowering healthcare professionals to help their patients more efficiently. (roche.com)
  • In this work, we have tested the human canonical members of the RNase family using a spot-culture growth inhibition assay based mycobacteria-infected macrophage model for evaluating their anti-tubercular properties. (frontiersin.org)
  • This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies. (jove.com)
  • The main advantage of this protocol is that it is less labor-intensive and time-consuming than the traditional method of counting colony forming units to estimate the intracellular growth of Mycobacterium tuberculosis. (jove.com)
  • PPO is often isolated from plant tissues in a latent form, which can be activated by a range of treatments, including proteolysis, aging, and incubation with detergents. (ashs.org)
  • Resistance mutations in Ng map to the N-terminal cone domain of the α subunit, which we show here is involved in forming an inhibited α 4 β 4 state in the presence of the β subunit and allosteric effector dATP. (elifesciences.org)
  • Typically, the polymerase chain reaction (PCR) is used to amplify a target sequence followed by a second assay to determine if the sequence contains a mutation associated with resistance, such as DNA sequencing or hybridization assays. (cdc.gov)
  • Guluronic acid residues can form so-called egg-box complexes with calcium ions or some other divalent metal cations leading to gel formation (Figure 1). (blogspot.com)
  • The name 'egg-box' is used because, if depicted schematically, the cations look like eggs situated inside puckered boxes formed by four guluronic acid residues of two superimposed chains. (blogspot.com)
  • Mannuronic acid residues have much less affinity to metal ions [1]. (blogspot.com)
  • Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
  • Our functional metagenomic screening of human fecal metagenomes yielded a novel β-farnesene synthase, which does not show amino acid sequence similarity to known β-farnesene synthases. (biomedcentral.com)
  • The novel DNA extraction protocol and specific E. coli K12 assay are sensitive and robust enough for detection and quantification within iron drinking water pipe biofilms, and are particularly well suited for studying enteric bacterial interactions within biofilms. (who.int)
  • C ) Outer membrane vesicle (OMV) quantification of WT and Δ mlaF deletion mutants harvested at 4 hr and quantified by measuring 3-deoxy-D- manno -oct-2-ulosonic acid (Kdo) levels using the Purpald assay. (elifesciences.org)
  • GPLs were separated in solvent system containing chloroform, methanol, and acetic acid (65:25:10, v/v/v). ( C ) LSC quantification of extracted GPLs. (elifesciences.org)
  • The sensitivity threshold for this PCR primer-based method was approximately 10 2 -10 3 colony forming units (CFU) per reaction, when H. pylori genomic DNA was used as a template. (biomedcentral.com)
  • In October 1981, Part II of the Guideline for Hospital Environmental Control, which contained the sections 'Housekeeping Services and Waste Disposal,' 'Laundry Services,' 'Intensive Care Units,' and 'Pharmacy,' was published. (cdc.gov)
  • METHODOLOGY: A cross-sectional study was performed by means of direct observation involving 60 Intensive Care Units (ICU) and clinical nursing professionals in a Brazilian hospital. (bvsalud.org)
  • Thanks to rapidly growing genomic and metagenomic sequencing data, high-throughput prediction approaches based on amino acid sequence similarity have emerged to efficiently screen potential TS genes [ 16 ]. (biomedcentral.com)
  • La présente étude menée dans un hôpital de soins tertiaires en Égypte a utilisé la méthode de PCR en temps réel pour évaluer la présence du gène blaKPC dans les isolats de K. pneumoniae non sensibles à l'ertapénème, puis a comparé les résultats à l'aide du test de Hodge modifié. (who.int)
  • A Cell Counting Kit-8 assay was used to test cell viability. (researchsquare.com)
  • These results provide information on the contamination problem of dental unit waterlines (DUWL) and indicate a need for treatment of the water used in dental units. (bvsalud.org)
  • OMV assays were performed in biological duplicate and quantified in technical triplicates. (elifesciences.org)
  • A ) Schematic of the biological principle behind the OMV pulse chase assay. (elifesciences.org)
  • Colony-forming unit assays yielded denser cell colonies in the HA treated samples compared to the controls, yet no difference in the number of colonies was detected, indicating that the number of stem cells remained unchanged. (europa.eu)
  • However, the iPS-like cells failed to form teratomas. (bvsalud.org)
  • Adipose tissue-derived MSCs had greater proliferative potential than bone marrow-derived MSCs, while no significantly difference in colony efficiency were observed between the two types of cells. (biomedcentral.com)
  • Phagocytosis of RAW 264.7 cells was evaluated by counting colony forming units. (researchsquare.com)
  • 1 , 2 In addition, mRNA encoding the cytokines interleukin 2 (IL-2) and granulocyte macrophage colony stimulating factor (GM-CSF) are increased at sites of disease activity, suggesting local activation of antigen specific T cells. (bmj.com)
  • Anodization leads to an oxidation of metal species that form a solid oxide on the metal surface. (hindawi.com)
  • The cross infection control in dental office has received great attention from professionals and one of the critical points is the bacteriological control of water used in dental unit. (bvsalud.org)
  • To perform a microbiological evaluation of the water used in dental units, the identification of Gram-negative non-fermentative rods (GNNR) and their ability to adhere to polystyrene, and the antimicrobial activity of disinfectants on the identified strains. (bvsalud.org)
  • Despite the efforts to avoid cross-infection in dental office using sterilized instruments, individual protection equipment and disinfection procedures, other measures such as microbiological control of the water used in dental units are required to prevent the spread of diseases 10,11 . (bvsalud.org)
  • The quality of water in dental units is of considerable importance because both patients and dental staff are regularly exposed to water and aerosol generated by these units 20,23 . (bvsalud.org)
  • Furthermore, until recently, there has been hardly any specific guidance from dental chair unit manufacturers on dental chair unit supply water quality 5 . (bvsalud.org)
  • Actually, the responsibility for ensuring that dental chair units provide good quality water output has, by and large, been considered to rest on the shoulders of dental practitioners and/or dental clinic management. (bvsalud.org)
  • The Brazilian Health Ministry Resolution n. 518 4 states that for water to be considered safe for human consumption, it should contain a maximum of 500 colony forming units per milliliter (CFU/ml), given it is free of coliforms. (bvsalud.org)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • Investigation of toxicity of Cu-Ti thin films was conducted the using the MTT assay, which revealed their biocompatibility and absence of cytotoxicity, further confirming their potential as promising biomaterials for various applications. (nature.com)
  • A single dental chair unit can be used in the treatment of many patients each day, and microbial contamination of specific component parts can be a significant potential source of cross-infection 10 . (bvsalud.org)
  • L -arginine is an essential amino acid for chickens and turns out to be a conditionally essential amino acid for mammals under many stressful conditions [ 4 , 5 , 6 ]. (biomedcentral.com)
  • 35 kg/m(2) each incremental BMI unit increase was associated with greater incremental total OR time increases. (stanford.edu)
  • Antibacterial activity of the prepared sample was assessed by determining the number of colony-forming units per milliliter (CFU/ml) using a standard viable cell count assay. (nature.com)
  • However, the tubercle bacillus may be present in a form without a cell wall and thus not detectable by conventional microbiological methods. (bmj.com)
  • Cellular arginine uptake is mainly mediated by the cationic amino acid transporter (CAT) family, also known as y + transporters, which is involved in active transport [ 8 ]. (biomedcentral.com)
  • These collectively form a thick, dense, poorly permeable cell wall, which not only allows mycobacteria to resist the dry environment and harmful chemicals but also allows it to reproduce in the macrophages [ 4 , 6 ]. (biomedcentral.com)
  • I Unidad de Investigación Médica en Inmunología e Infectología, Hospital de Infectología, Centro Médico Nacional La Raza (CMNR), Instituto Mexicano del Seguro Social (IMSS). (scielo.org.mx)
  • There are currently no official standards or legislation regarding microbial quality of dental unit waterlines (DUWL). (bvsalud.org)
  • RÉSUMÉ L'émergence et la propagation rapide des souches de Klebsiella pneumoniae résistantes aux antibiotiques et porteuses du gène blaKPC codant la production de carbapénèmases ont compliqué la prise en charge des infections des patients. (who.int)
  • Ordered nanotubular structures of TiO 2 , with a controlled and uniform diameter, length, and wall thickness, can be formed if the dissolution is enhanced by fluoride containing electrolytes and suitable anodization conditions are used [ 14 ]. (hindawi.com)
  • TBM must be differentiated not only from other forms of acute and subacute meningitis but also from conditions such as viral infections and cerebral abscess. (medscape.com)