Acceptor glycosylation. Medical search. Frequent questions

Hypoglycosylation is the hallmark of a group of rare genetic diseases called congenital disorders of glycosylation (CDG). (nih.gov)
Reduced or altered transferrin glycosylation is a common sign of N-linked congenital disorders of glycosylation. (cdghub.com)
Carbohydrate deficient transferrin is commonly found in N-linked congenital disorders of glycosylation. (cdghub.com)

The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. (nih.gov)
This protein may function as a follow-up enzyme in the initiation step of O-glycosylation. (nih.gov)
Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)
Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
Roitsch T, Lehle L. Structural requirement for protein glycosylation. (rusmedserv.com)
The N- glycosylation is an essential protein modification taking place in the membranes of the endoplasmic reticulum (ER) in eukaryotes and the plasma membranes in archaea. (biomedcentral.com)
The origin of the eukaryotic N- glycosylation pathway is not unique and less straightforward than previously thought: some basic components likely have proteoarchaeal origins, but the pathway was extensively developed before the eukaryotic diversification through multiple gene duplications, protein co-options, neofunctionalizations and even possible horizontal gene transfers from bacteria. (biomedcentral.com)
A genetically heterogeneous group of heritable disorders resulting from defects in protein N-glycosylation. (lookformedical.com)
Flippases are also essential for transporting lipid-linked oligosaccharides ("LLOs"), which are transferred onto acceptor proteins during N-linked protein glycosylation. (chemeurope.com)
The glucosylphosphotransferase acceptor in rat liver is also immunoprecipitated by the anti-parafusin antibody and is very similar in physical characteristics to the paramecium protein. (elsevierpure.com)
Some important glycosides, the glycosyl phosphopolyprenols , are involved in several reactions leading to protein glycosylation. (gerli.com)

Unlike other family members, this enzyme shows exclusive specificity for partially GalNAc-glycosylated acceptor substrates and shows no activity with non-glycosylated peptides. (nih.gov)
A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates. (nih.gov)

N-glycans are covalently linked to an asparagine residue in a simple acceptor sequence of proteins, called a sequon. (nih.gov)
However, N-glycosylation of platelet proteins has been poorly investigated in CDG. (nih.gov)
Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)
Asparagine-linked glycosylation of proteins occurs within the lumen of the RER. (umassmed.edu)
The comparison of several polyisoprenol-based glycosylation pathways from the three domains of life shows that most of the implicated proteins belong to a limited number of superfamilies. (biomedcentral.com)
More than 10,000 glycosylation sites in various proteins have been identified in humans. (chemeurope.com)
A large family of GalNAc-Ts initiate mucin type O-glycosylation transferring α-GalNAc from a UDP-GalNAc donor to the hydroxyl groups of Ser and Thr residues of peptides and proteins, thereby defining sites of O-glycosylation. (araid.es)

Current evidence indicates that the STT3A isoform of the OST is primarily responsible for cotranslational glycosylation of nascent glycoproteins as the acceptor sequons exit the luminal face of the translocation channel. (umassmed.edu)
Influence of acceptor peptides on cotranslational glycosylation of yeast invertase and site-directed mutagenesis around a sequon sequence. (rusmedserv.com)

Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. (lookformedical.com)
The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme. (ucm.es)
Glycosylation can have a profound effect on the biological and physicochemical properties of a molecule. (ugent.be)
Enzymes that transfer an amino group from an amino acid to an acceptor molecule, typically an alpha-ketoacid. (cdghub.com)

A chemical glycosylation reaction involves the coupling of a glycosyl donor, to a glycosyl acceptor forming a glycoside. (wikipedia.org)
If both the donor and acceptor are sugars, then the product is an oligosaccharide. (wikipedia.org)
The glycosylation reaction involves the coupling of a glycosyl donor and a glycosyl acceptor via initiation using an activator under suitable reaction conditions. (wikipedia.org)
The stereochemical outcome of a glycosylation reaction may in certain cases be affected by the type of protecting group employed at position 2 of the glycosyl donor. (wikipedia.org)
Different protecting groups on either the glycosyl donor or the glycosyl acceptor may affect the reactivity and yield of the glycosylation reaction. (wikipedia.org)
Typically, electron-withdrawing groups such as acetyl or benzoyl groups are found to decrease the reactivity of the donor/acceptor and are therefore termed "disarming" groups. (wikipedia.org)
Electron-donating groups such as the benzyl group, are found to increase the reactivity of the donor/acceptor and are therefore called "arming" groups. (wikipedia.org)
We have found that generation of electron-donor-acceptor (EDA) complexes between thioglycosides (and related substrates) and sulfonium ions serves as a prerequisite to effective photochemical activation and subsequent glycosylation. (bsu.edu)
The major findings were, that HEX1 and HEX2 catalyze trans-glycosylation reactions with lactose as acceptor, giving rise to the human milk oligosaccharide precursor lacto-N-triose II (LNT2) with yields of 2 and 8 % based on the donor substrate. (dtu.dk)
Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid). (ucm.es)
Electrochemical glycosylation of a selenoglycoside donor proceeds efficiently in an undivided cell in acetonitrile to yield beta-glycosides. (ox.ac.uk)
Alternatively, stepwise glycosylation of 12 with a D donor compatible with a selective deblocking at position 3(D) and a 2-O-acetyl C donor following exposure of OH-3(D) led to a pentasaccharide, which was partially and fully deprotected into free (Ac)CD(E)AB and CD(E)AB, respectively. (pasteur.fr)
Furthermore, chain elongation of the common D(E)AB acceptor with a 2(B)-O-levulinoyl rhamnobiose donor BC and subsequent partial or total deprotection of the resulting hexasaccharide provided B(Ac)CD(E)AB and BCD(E)AB, respectively. (pasteur.fr)

Glycosyl iodides were first introduced for use in glycosylation reactions in 1901 by Koenigs and Knorr although were often considered too reactive for synthetic use. (wikipedia.org)
Thioglycosides used in glycosylation reactions as donors can be activated under a wide range of conditions, most notably using NIS/AgOTf. (wikipedia.org)
Importantly, all of these enzymes catalyzed N -glycosylation reactions in vitro with no purification or processing needed. (biorxiv.org)
In total, trans-glycosylation reactions were tested with the disaccharide acceptors β-lactose, sucrose, and maltose, as well as with the monosaccharides galactose and glucose resulting in the successful attachment of GlcNAc to the acceptor in all cases. (dtu.dk)
Several types of carbohydrate-active enzymes (CAZymes) may be used in glycosylation reactions. (researchsquare.com)
In this project, glycosylation reactions catalysed by GH-13 enzymes will be analysed by the systematic mutagenesis of aminoacids in their active site. (ugent.be)

In the present study the role of flanking charged residues was investigated using a library of charged peptide substrates containing the central -YAVTPGP- acceptor sequence. (araid.es)
NADH-cytochrome b5 reductase is capable of reducing different types of electron acceptors: natural, seminatural, and artifical (xenobiotic) substrates. (bomiprot.org)

We recently discovered that phenanthroline could be used as a nucleophilic catalyst to access high yielding and diastereoselective α-1,2-cis glycosides through the coupling of hydroxyl acceptors with α-glycosyl bromide donors. (wayne.edu)
The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid. (ucm.es)
La glyco-enzymologie au travers de laquelle l'équipe cherche à identifier, à caractériser et à modifier les propriétés d'enzymes impliquées dans le métabolisme des sucres (glycosides hydrolases, sulfatases) pour les utiliser en tant qu'outils synthétiques pour la production de molécules à haute valeur ajoutée. (univ-nantes.fr)

A glycosyl acceptor is a sugar with an unprotected nucleophilic hydroxyl group which may attack the carbon of the oxocarbenium ion formed during the reaction and allow for the formation of the glycosidic bond. (wikipedia.org)
Koenigs-Knorr glycosylation of acceptors with more than one free hydroxyl group by 2,3,5,6-tetrabenzoyl galactofuranosyl bromide was performed using diphenylborinic acid 2-aminoethyl ester (DPBA) as inducer of regioselectivity. (researcher-app.com)
High regioselectivity for the glycosylation on the equatorial hydroxyl group of the acceptor was obtained thanks to the transient formation of a borinate adduct of the corresponding 1,2- cis diol. (researcher-app.com)

Additionally thioglycosides act as temporary protecting groups at the anomeric position allowing for thioglycosides to be useful as both glycosyl donors as well as glycosyl acceptors. (wikipedia.org)
A variety of disaccharides are readily synthesised in high yield, but limitations of the use of selenoglycosides as glycosyl donors for selective glycosylation of thioglycoside acceptors are exposed. (ox.ac.uk)

GalNAc-T isozyme surface charge governs charge substrate preferences to modulate mucin type O-glycosylation. (araid.es)
We previously showed that the GalNAc-Ts possess common and unique specificities for acceptor type, peptide sequence and prior neighboring and/or remote substrate GalNAc glycosylation. (araid.es)

These limitations include a lack of available tools for biochemical characterization of enzymes involved in glycosylation. (biorxiv.org)
Based on the obtained information, the enzymes will subsequently be optimised through enzyme engineering to increase their activity towards non-carbohydrate acceptors. (ugent.be)
Les interactions sucres/protéines (enzymes, lectines) sont au cœur de la communication cellulaire tant extracellulaire (interactions cellule-cellule, cellule-matrice, hôte-pathogène) qu'intracellulaire (GlcNAcylation en tant que modification post-traductionnelle). (univ-nantes.fr)
The glycosylation machinery is responsible for assembling a diverse repertoire of glycan structures, collectively termed 'glycome', through the synchronized action of a portfolio of glycan-modifying enzymes including glycosyltransferases and glycosidases. (nature.com)

For the past 12 years, my research group has been interested in the development of approaches to chemical glycosylation that leverage new and unusual forms of reactivity. (bsu.edu)
Selective electrochemical glycosylation by reactivity tuning. (ox.ac.uk)

It appears likely that the dephosphorylation observed with secretion reflects the removal of αGlc-1-P from parafusin's oligosaccharides and is consistent, therefore, with a regulatory role for this cytoplasmic glycosylation event. (elsevierpure.com)

This avoided the multi-step synthesis of a more complex disaccharide acceptor 3, greatly simplifying the route to tetrasaccharide target 2. (gazi.edu.tr)
Interestingly electron-withdrawing groups on O -6 or on C -1 of the acceptor displaced the reaction in favor of the desired galactofuranosyl containing disaccharide. (researcher-app.com)

It shares mechanistic similarities based on the use of polyisoprenol lipid carriers with other glycosylation pathways involved in the synthesis of bacterial cell wall components (e.g. peptidoglycan and teichoic acids). (biomedcentral.com)
Paramasivam, S. & Fairbanks, A. J. Synthesis and semisynthesis of N-glycans and ENGase catalysed glycosylation using Fmoc-Asn-GlcNAz as acceptor, Carbohydrates Gordon Research Conference 2019, Hong Kong. (ac.lk)

To better understand this, the mechanism of a glycosylation reaction must be considered. (wikipedia.org)
A monosaccharide 5, N-acetyl-alpha-D-glucosamine- O-methylglycoside (GlcNAc-OMe), was successfully used as an acceptor in a heparosan synthase (pmHS2)-catalyzed glycosylation reaction. (gazi.edu.tr)
The synthetic strategy will utilize the β-hydroxyl groups on the pentofuranosides as guiding groups for stereospecific C-glycosylation reaction. (ntnu.edu.tw)

Defects in N-glycosylation and O-glycosylation constitute the largest CDG groups. (nih.gov)
Traditionally, CDG were classified as either Type I or Type II CDG, but this classification system can only differentiate CDG caused by defects in N-glycosylation and is no longer used to classify CDG types. (cdghub.com)

The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure-activity relationship study. (ucm.es)

Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms. (umassmed.edu)

In this review, we highlight normal and deficient N-glycosylation of platelet-derived molecules and discuss the involvement of platelets in the congenital disorders of N-glycosylation. (nih.gov)

which catalyses transfer of saccharidic residue to the appropriate acceptor. (sav.sk)

Use of an EAB intermediate orthogonally protected at 2(A) provided both the trisaccharide target and acceptor 12, the condensation of which with a chain terminator D followed by full deprotection, gave tetrasaccharide D(E)AB. (pasteur.fr)

Is there a hallmark 'glycosylation signature' that characterizes the initiation, execution and resolution of cell death programs in physiologic and pathologic settings? (nature.com)

Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)

Oligosaccharyltransferase (OST) catalyzes the transfer of a preassembled high-mannose oligosaccharide onto N-X-T or N-X-S acceptor sites (seqons) as the nascent polypeptide is transported into the RER lumen through the Sec61 complex. (umassmed.edu)

Following a thorough investigation on the use of N-trichloroacetylglucosamine- versus N-acetylglucosamine-containing tri- and tetrasaccharide acceptors, the successful strategy was based on an efficient combination of the trichloroacetimidate chemistry, a trichloroacetyl used as permanent N-protection, and an allyl aglycon as temporary and/or permanent anomeric protection of selected building blocks. (pasteur.fr)

With the latter acceptor, we were able to isolate and characterize one glycosylation product in a low yield. (ucm.es)

The objective of this proposal is to explore generally applicable approaches and potentially new strategies for the stereoselective C-glycosylation of pentofuranose derivatives. (ntnu.edu.tw)

The most common technique is transferrin isoelectric focusing, but mass spectrometry is increasingly used to analyze different glycosylation patterns on transferrin in greater detail. (cdghub.com)

Here, a phylogenomic analysis was carried out to examine the validity of rival hypotheses suggesting alternative archaeal or bacterial origins to the eukaryotic N- glycosylation pathway. (biomedcentral.com)

In this proposal, we will start with the evaluation of an intramolecular strategy for the nucleosidic bond formation to control the stereochemistry and enhance the reactivities for C-glycosylation. (ntnu.edu.tw)

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