• We have shown that the mer determinant can either be located at the chromosome, or on a plasmid in the Bacillus species, and is carried by class II mercury resistance transposons: Tn5084 from B. cereus RC607 and B. cereus VKM684 (ATCC10702) and Tn5085 from Exiguobacterium sp. (dadospdf.com)
  • They often participate in lateral gene transfer, which is more or less one microbe extending a cytoskeletal shiv and shaking down neighboring microbes for plasmids or transposons. (futurzweb.com)
  • Tn5085 is identical in nucleotide sequence to TnMERI1, the only other known mer transposon from Bacillus species, but it does not contain an intron like TnMERI1. (dadospdf.com)
  • Your primer may have a secondary priming site on the plasmid that may be identical or closely related, with different nucleotide sequences following each site, giving superimposed bands within your sequence. (etonbio.com)
  • Tn5083, which we have isolated from B. megaterium MK64-1, contains an RC607-like mer determinant, that has lost some mercury resistance genes and possesses a merA gene which is a novel sequence variant that has not been previously described. (dadospdf.com)
  • The sequence data showed evidence for recombination both between transposition genes and between mer determinants. (dadospdf.com)
  • 1981. Jones and Woods (1986) have thoroughly documented its history of use … This plasmid seems to be contain nearly all genes involved with solvent production and is aptly named pSOL1. (thecoffeeparlor.com)
  • Sometimes, though, the plasmid thieves get more than they bargained for: Some genes make bacteria immune to one drug, but just because they have the sequence they do, they make the bacteria susceptible to another drug. (futurzweb.com)
  • Sequence analyses of unigenes revealed four potential groups: hydrolases, cell envelope associated proteins (CEAPs), candidate effectors and other proteins. (biomedcentral.com)
  • The three affected patients were extensively evaluated with metagenomic sequencing for infectious pathogens, exome sequencing, spinal fluid immune analyses, neurometabolic and toxicology testing, continuous electroencephalography, and neuroimaging. (cdc.gov)
  • Resistance to mercury salts is found in many genera of bacteria isolated from the environment, and many mer determinants have been sequenced. (dadospdf.com)
  • Edited by Mark Hower, student of Rachel Larsen and Kit Pogliano, From MicrobeWiki, the student-edited microbiology resource, "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. (thecoffeeparlor.com)
  • During the normal course of cell division, a bacterium can sort of ditch the more expensive plasmids they've been keeping around, leaving only those cells alive that can handle the scarcity. (futurzweb.com)
  • Health Canada Not Concerned About Scientists' Finding of Plasmid DNA Contamination in COVID ShotsAfter two scientists independently raised red flags about an inordinate amount of plasmid DNA in the COVID-19 mRNA shots-which they say could potentially alter the human genome-Health Canada did not express concern with the findings, given the testing data provided by the manufacturers. (shtf.tv)
  • Mr. McKernan warned that the presence of DNA plasmids in the vaccines meant they could potentially integrate into the human genome. (shtf.tv)
  • Exome sequencing did not identify potentially causal candidate gene variants. (cdc.gov)
  • Drugs like -cyclines and -mycins interfere with protein production, which brings the whole cell abruptly to a stop. (futurzweb.com)
  • Although chromosomal integration of the plasmid DNA was initially a major theoretical concern, the data obtained to date do not support this concern," it says. (shtf.tv)
  • The two most common causes for failure to get good or any sequence data for your samples are concentration and purity of your template DNA. (etonbio.com)
  • If it appears that you have done everything correctly and followed our suggestions, then look below for some additional reasons why you might obtain less than optimal DNA sequence data quality. (etonbio.com)
  • If you've designed your own custom primer from previous sequence data, make sure you were using a reliable area of sequence - look for sharp, well-defined peaks with no ambiguity. (etonbio.com)
  • You may need to reprep your sample to sufficiently remove one or more inhibitory components to obtain any sequence data. (etonbio.com)
  • Health Canada was aware of the presence of residual plasmid DNA as a process-related impurity during review and prior to the authorization of the mRNA COVID-19 vaccines," the agency said in an email to The Epoch Times. (shtf.tv)
  • In April, he published a paper documenting his research in which he used several methods to test four expired Pfizer and Moderna COVID-19 vaccine vials-which were thought to only contain mRNA-and discovered the presence of double-stranded DNA plasmids. (shtf.tv)
  • The presence of residual plasmid DNA in the mRNA COVID-19 vaccines does not change the safety assessment of these vaccines by Health Canada," says Health Canada. (shtf.tv)
  • The cycle sequencing reaction used to amplify samples for automated sequencing is very sensitive to the presence of certain contaminants, some of which will completely inhibit our sequencing enzyme. (etonbio.com)
  • The presence of multiple peaks within your sequence can be caused by numerous factors. (etonbio.com)
  • If you are having trouble getting good sequencing results for your samples, you may first want to look through our How to Prepare Samples section for some recommendations on template preparation and quantitation. (etonbio.com)
  • DNA polymerases isolated from cells and artificial DNA primers can be used to start DNA synthesis at known sequences in a template DNA molecule. (wikipedia.org)
  • The nucleotide sequence of the PLF-1 cDNA revealed a single open reading frame encoding a protein of about M, 24,000 with clear homology to preprolactin (2). (nih.gov)
  • By convention, if the base sequence of a single strand of DNA is given, the left end of the sequence is the 5′ end, while the right end of the sequence is the 3′ end. (wikipedia.org)