Zinc finger dna binding proteins. Medical search. Frequent questions

Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. (nih.gov)
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. (embl.de)
They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)
Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. (embl.de)
DNAbinding proteins combine with DNA through several motifs called zinc fingers, zinc twists, ring fingers, or zinc clusters. (dojindo.com)
LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. (thermofisher.com)
DEAF-1 recognizes several TTCG motifs within the portion of the Dfd autoregulatory region termed 'module E.' In addition, DEAF-1 binds several similar motifs within a Dfd response element ( DRE ) from the 1.28 gene that enhances maxillary gene expression during embryogenesis (Pederson, 2000). (sdbonline.org)
We created several deletion mutants of this protein based on its conserved motifs, and from these structures functional chimeras were synthesized, supporting the hypothesis that gene duplication and recombination could lead to novel functional forms of single-stranded DNA-binding proteins. (johnshopkins.edu)
The DNA-binding domain (DBD) of the hGRα corresponds to amino acids 420-480 and contains 2 zinc finger motifs through which the hGRα binds to specific DNA sequences, the glucocorticoid-response elements (GREs) in the promoter region(s) of target genes. (medscape.com)
The 2 zinc finger motifs are able to tetrahedrally coordinate a zinc atom and are held by 4 cysteine (Cys) residues (see the image below, panel C). (medscape.com)
Leucine zipper motifs are usually found as part of a DNA-binding domain in many transcription factors , and are therefore these motifs are involved in regulating gene expression . (vigyansarovar.com)

This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. (nih.gov)
The requirement for a total of 6-12 fingers, each of which binds three base pairs, provides enough specificity to address a unique sequence in a genome as complex as the three billion base pairs of human DNA. (ddw-online.com)
We have performed comprehensive analysis of homeodomain and Cys2His2 zinc finger specificities in D. melanogaster - (fruit fly) in collaboration with Michael Brodsky (UMMS-MCCB) & Gary Stormo (Wash. U). We have coupled this data with specificity data from artificial zinc fingers and homeodomains that we have selected using our bacterial one-hybrid system. (umassmed.edu)
Using this information we can build simple qualitative models of recognition that allow the design of homeodomains with novel DNA-binding specificity. (umassmed.edu)
This dataset can also be used to broadly predict the specificity of homeodomains ( http://stormo.wustl.edu/flyhd/ ) and zinc fingers ( http://stormo.wustl.edu/ZFModels/ ). (umassmed.edu)
2012 ), generalizing the observation that hotspot localization is determined by the DNA-binding specificity of the zinc finger array (Grey et al. (springer.com)

Our data rule out a model suggesting that xKaiso is a direct repressor of Wnt signalling target genes in early Xenopus development via binding to promoter-proximal CTGCNA sequences as part of a xTcf3 repressor complex. (biologists.com)
The new tools that have opened the door to facile manipulation of genomic DNA sequences are enzymes that can be designed to cut DNA specifically at essentially any arbitrarily chosen sequence. (ddw-online.com)
We isolated 29 unique ZFP sequences of which zinc proliferation 1 (Zipro1) was the most abundant. (tamu.edu)
Parallel-stranded DNA with natural base sequences. (mpg.de)
As a result, tests in RNA/DNA sequences have become more accessible and affordable. (explorebiotech.com)

Exons 1-6 of the WT1 gene encode the regulatory domain, which regulates expression of target genes, and exons 7-10 encode the 4 zinc fingers of the DNA-binding region of the WT1 protein. (medscape.com)
The WT1 protein mediates the mesenchymal-epithelial transition and differentiation during morphogenesis of the kidney and gonad by repressing genes that encode cell proliferation factors and by activating genes that encode markers of epithelial cell differentiation. (medscape.com)
We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
Most unexpectedly, genes coding for similar proteins were found in the genomes of eukaryotes, including humans. (johnshopkins.edu)
At the cellular level, the actions of glucocorticoids are mediated by a 94-kd protein, the glucocorticoid receptor (GR). The human (h) GR belongs to the steroid/thyroid/retinoic acid superfamily of nuclear receptors and functions as a ligand-dependent transcription factor that regulates the expression of glucocorticoid-responsive genes positively or negatively. (medscape.com)
We found that the acidic domain of MZF-1 and the heparin-binding domain of Elk-1 facilitate the heterodimeric interaction between the two genes before the complex formation binds to the PKCα promoter. (oncotarget.com)
[7] Removing DNA methylation marks allows the activation of downstream genes. (wikipedia.org)
1999). Molecular cloning of six novel Kruppel-like zinc finger genes from hematopoietic cells and identification of a novel transregulatory domain KRNB. (geneticsmr.com)

Targeting a DNA binding motif of the EVI1 protein by a pyrrole-imidazole polyamide. (rochester.edu)
In the proposed "zinc finger" DNA-binding motif, each repeat unit binds a zinc metal ion through invariant Cys and His residues and this drives the folding of each 30-residue unit into an independent nucleic acid-binding domain. (embl.de)
Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. (thermofisher.com)
SELEX assays and footprinting data indicate that DEAF-1 binds to and activates Mtk and Drs regulatory DNAs via a TTCGGBT motif. (sdbonline.org)
The term 'motif' is also describes a consensus sequence of amino acids, i.e. a partial sequence common to a number of different proteins, which may or may not adopt similar conformations in the different proteins. (vigyansarovar.com)
Helix-turn-helix motif are involved in many processes that includes DNA repair, RNA metabolism, and protein-protein interaction . (vigyansarovar.com)
Unlike HTH proteins, the actual DNA-binding motif of the HLH proteins is a stretch of about 13 "basic" amino acids adjacent to the HLH whose main role is in protein dimerization. (vigyansarovar.com)
Therefore, entire motif comprising the DNA-binding basic region and the HLH is termed bHLH . (vigyansarovar.com)

We found that after CST transection, levels for Zipro1 mRNA doubled and that Zipro1 protein expression increased in non-myelinating CST SCs. (tamu.edu)
Alternative splicing of the primary transcript gives rise to the 2 mRNA and protein isoforms, hGR-alpha and hGR-beta. (medscape.com)
The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. (idhinhibitor.com)
Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)

Our data demonstrate expression of replacement enzymes for multiple different proteins, including those deficient in lysosomal storage disorders, which serves to demonstrate the potential of this approach for a broad range of other monogenic diseases. (medicalmvp.com)
Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)

We hypothesize that zinc-finger transcription factors could play a role during tSC activation. (tamu.edu)
The AF-1 plays an important role in the interaction of the receptor with molecules necessary for the initiation of transcription, such as coactivators, chromatin modulators, and basal transcription factors, including RNA polymerase II, TATA-binding protein (TBP), and a host of TBP-associated proteins (TAFIIs). (medscape.com)
These helix-turn-helix structure found in many proteins(transcription factors). (vigyansarovar.com)
In general, transcription factors including this domain are dimeric, each with one helix that contains basic amino acid residues facilitate DNA binding. (vigyansarovar.com)
The SCAN domain family of zinc finger transcription factors. (geneticsmr.com)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

This gene encodes a member of the Kruppel family of zinc finger proteins. (wikipedia.org)
The Snail gene family encodes DNA-binding zinc finger proteins that function as transcriptional repressors. (plos.org)
Background: This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. (idhinhibitor.com)

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. (nih.gov)
The others are the site-specific TFs or the DNA sequence-specific binding proteins. (intechopen.com)
however, finger 3 is non-sequence-specific. (sinica.edu.tw)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)

The interaction of Kaiso with xTcf3 is highly conserved and is dependent on its zinc-finger domains (ZF1-3) and the corresponding HMG DNA-binding domain of TCF3/4 factors. (biologists.com)
LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. (thermofisher.com)
The two amphipathic helices, particularly the highly conserved hydrophobic amino acids, are thought to mediate dimerization, which is a prerequisite to DNA binding. (vigyansarovar.com)

It is also a company to conduct clinical trials with gene-edited T cells and use zinc finger nuclease technology. (explorebiotech.com)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

The architecture of single-stranded DNA-binding proteins, which play key roles in DNA metabolism, is based on different combinations of the oligonucleotide/oligosaccharide binding (OB) fold. (johnshopkins.edu)
1995. Copper and zinc metabolism in health and disease: Speciation and interactions. (cdc.gov)

Members of this DNA-binding protein family act as transcriptional regulators. (wikipedia.org)
EVI1 Interferes with Myeloid Maturation via Transcriptional Repression of Cebpa, via Binding to Two Far Downstream Regulatory Elements. (rochester.edu)
It is a nuclear protein and functions as a transcriptional regulator. (wikipedia.org)

The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)

The human EGR-1 protein contains (in its unprocessed form) 543 amino acids with a molecular weight of 57.5 kDa , and the gene is located on the chromosome 5 . (wikipedia.org)

Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)
The functional domains and subdomains are indicated beneath the linearized protein structures. (medscape.com)

Photophysics of green and red fluorescent proteins: Implications for quantitative microscopy. (mpg.de)

The effects of DNA methylation and the histone code are due, at least in part, to modification-specific recruitment of factors, such as heterochromatin-associated proteins (HP1) and methyl-binding domain proteins, which establish and maintain higher order of chromatin structure. (aacrjournals.org)
DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)

Cross-talk between different histone modifications, as well as DNA methylation, seems complex. (aacrjournals.org)
In mammalian cells, histone H3 Lys 9 (H3K9) methylation and histone hypoacetylation are usually associated with methylated DNA, heterochromatin, and gene silencing. (aacrjournals.org)
Histone hyperacetylation and H3K4 methylation are associated with unmethylated DNA, euchromatin, and gene expression ( Fig. 2 ). (aacrjournals.org)
This role involves its DNA-binding domain, which is composed of a tandem array of zinc fingers, and PRDM9-dependent trimethylation of histone H3 at lysine 4. (springer.com)
2010 ). PRDM9 includes several functional domains, such as a DNA-binding zinc finger array, a histone lysine methyltransferase PR/SET (PRDI-BF1 and RIZ1 homology) domain, and a Krüppel-associated box (KRAB)-related domain. (springer.com)

They have yielded positive results from their combined strategy of adjusting the binding affinity of the zinc finger array and slowing the catalytic rate of the Fok1 cleavage domain. (explorebiotech.com)

In the auxin and jasmonic acid systems, ligand recognition is shared between protein-binding partners. (nature.com)
The human GR is a modular protein composed of distinct regions illustrated in panel B in the image below, as follows: (1) The amino-terminal A/B region, also called immunogenic or N-terminal domain (NTD) and (2) the C, D, and E regions, which correspond to the DNA-binding domain, the hinge region, and the ligand-binding domain, respectively. (medscape.com)

Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)
We are in the midst of a revolution in genome engineering, based on reagents that can be designed to cut chromosomal DNA at arbitrary sites. (ddw-online.com)
Creating more precise CRISPR/Cas9 nucleases for gene therapy - We are utilzing our expertise in protein engineering and protein-DNA recognition to create hybrid CRISPR/Cas9 nucleases to achieve single-site cleavage precision within the human genome. (umassmed.edu)
Perfecting the use of zinc-finger nucleases (ZFNs), this company has enhanced genome editing technology. (explorebiotech.com)
In addition to producing cost-effective and applicable crop species, this company aims to develop mammalian protein and human drugs at cost-effective prices using the genome-edited plant platform. (explorebiotech.com)
Based on Sangamo's zinc finger DNA-binding protein (ZFP) genome-editing technology, the platform enables the permanent production of therapeutic proteins from a specific genomic site in the liver with a single systemic treatment, potentially providing curative treatments for a range of monogenic diseases such as hemophilia and lysosomal storage disorders (LSD) including Gaucher and Fabry disease. (medicalmvp.com)
These data provide proof of concept for this broadly applicable genome editing strategy by demonstrating that our process is scalable to large animals and by validating the use of the albumin safe harbor as a site for expression of therapeutic proteins," said Philip Gregory. (medicalmvp.com)
Our In Vivo Protein Replacement Platform is a highly leverageable and disruptive application of our ZFN-mediated genome editing technology and can potentially be applied to any disease-relevant gene where enabling the liver to provide a stable source of corrective replacement protein will be therapeutic. (medicalmvp.com)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

They contain a short beta hairpin and an alpha helix (beta/beta/alpha structure), where a single zinc atom is held in place by Cys(2)His(2) (C2H2) residues in a tetrahedral array. (embl.de)
The protein-DNA interaction is stabilized by nonspecific contacts between the DNA backbone and residues in α 2 and the turn between α 2 and α 3. (vigyansarovar.com)
The residues marked 'x' are not part of the zinc fingers, but rather serve to connect them all together. (wikipedia.org)

1990. A review on biochemical roles, toxicity and interactions of zinc, copper and iron: IV. (cdc.gov)

Here we show that unlike these groups of organisms, the Euryarchaeota has exploited the potential in the OB fold to re-invent single-stranded DNA-binding proteins many times. (johnshopkins.edu)
Most interestingly, a second single-stranded DNA-binding protein, FacRPA2, in this archaeon exhibited the wrapping mode. (johnshopkins.edu)
Although the diversity shown by archaeal single-stranded DNA-binding proteins is unparalleled, the presence of their simplest form in many organisms across all domains of life is of greater evolutionary consequence. (johnshopkins.edu)
The protein has a preference for binding single stranded DNA and RNA. (idhinhibitor.com)

The first of the targetable cleavage reagents to be developed were the zinc-finger nucleases (ZFNs) (1), which consist of a non-specific cleavage domain from a restriction endonuclease linked to a DNArecognition domain made up of zinc fingers. (ddw-online.com)
Sangamo's In Vivo Protein Replacement Platform makes use of a highly expressed and liver specific genomic "safe-harbor site" that can be edited with ZFP nucleases (ZFNs) to accept and express any therapeutic gene and thus permanently produce high levels of the missing protein with a single treatment. (medicalmvp.com)
Scientists demonstrated efficient ZFN-mediated gene addition into the albumin locus in adult mouse models of hemophilia A and B with a single systemic administration of ZFNs and a donor DNA sequence encoding a corrective human Factor VIII or IX gene, respectively. (medicalmvp.com)

When a break is made in genomic DNA, cells hasten to repair the damage using two major pathways (Figure 1). (ddw-online.com)
It takes two ZFN monomers to make a break, each of which carries three to six fingers chosen to bind the desired genomic target (Table 1). (ddw-online.com)

Znf domains are often found in clusters, where fingers can have different binding specificities. (embl.de)
There are 2594488 ZnF_C2H2 domains in 441262 proteins in SMART's nrdb database. (embl.de)
To obtain structural information, we synthesized single and double zinc finger peptides from the yeast transcription activator ADR1, and assessed the metal-binding and DNA-binding properties of these peptides, as well as the solution structure of the metal-stabilized domains, with the use of a variety of spectroscopic techniques. (embl.de)
The 576-aa DEAF-1 protein possesses two conserved domains, SAND and MYND. (sdbonline.org)
The MG132 proteasome inhibitor, and knockdown of checkpoint with forkhead and ring finger domains (CHFR) and ring finger protein 146 (RNF146), E3 ubiquitin ligases targeting PARP1, suppressed nutlin-3a-induced PARP1 reduction. (oncotarget.com)
The DNA-binding domain of EGR-1 consists of three zinc finger domains of the Cys 2 His 2 type. (wikipedia.org)
The crystal structure of DNA bound by the zinc finger domain of EGR-1 was solved in 1991, which greatly aided early research in zinc finger DNA-binding domains. (wikipedia.org)

1992. Auditory event-related potential (P300) in relation to peripheral nerve conduction in workers exposed to lead, zinc, and copper: Effects of lead on cognitive function and central nervous system. (cdc.gov)

Using affinity chromatography coupled to multidimensional protein identification technology (MudPIT), DEAF-1 was identified as a candidate regulator. (sdbonline.org)
DNA affinity precipitation and mass spectroscopy of rs147978008 revealed allele-dependent binding of H1 histones, which was confirmed by using Western blotting. (cdc.gov)

Recent reports demonstrate that the expression of protein kinase C alpha (PKCα) in triple-negative breast cancer (TNBC) correlates with decreased survival outcomes. (oncotarget.com)
Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. (geneticsmr.com)
1999). p38 mitogen-activated protein kinase inhibitors-mechanisms and therapeutic potentials. (geneticsmr.com)

PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)
In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)

Identification of binding sites of EVI1 in mammalian cells. (rochester.edu)

Electrophoretic mobility shift assays detected allele-dependent nuclear protein binding in A549 cells for 8 of 21 variants. (cdc.gov)

The process of TZD binding to the cognate DNA is likely stepwise: initially TZD non-specifically binds to DNA, then fingers 1 and 2 insert cooperatively into the major groove of DNA by semi-specific binding, and finally finger 3 non-specifically binds to DNA, which promotes the specific binding on fingers 1 and 2 and stabilizes the formation of a specific TZD-DNA complex. (sinica.edu.tw)
α 3 is recognition helix that binds to DNA major groove through a series of hydrogen bonds and various Vander wall forces with exposed bases. (vigyansarovar.com)

PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)

Zinc finger protein 165 is a protein that in humans is encoded by the ZNF165 gene. (wikipedia.org)
Zinc is an essential metal ion, and its level is reported to be 28.5 mg per 1 g tissue in humans. (dojindo.com)
In humans and mice, hotspots are specified by PRDM9, a meiosis-specific PRDM family protein (Baudat et al. (springer.com)
EGR-1 (Early growth response protein 1) also known as ZNF268 (zinc finger protein 268) or NGFI-A (nerve growth factor-induced protein A) is a protein that in humans is encoded by the EGR1 gene . (wikipedia.org)

DNA binding), and others can be the part of larger structural assemblies. (vigyansarovar.com)
Example-appear in each structural domain of the signalling protein calmodulin and in the muscle protein troponin-C . (vigyansarovar.com)
These enzymes modify histones, which are structural proteins that attach (bind) to DNA and give chromosomes their shape. (medlineplus.gov)

Induction of zinc-finger proliferation 1 expression in non-myelinating Schwann cells after denervation. (tamu.edu)
1994. Inhibition of Na+-glucose cotransport in kidney cortical cells by cadmium and copper: Protection by zinc. (cdc.gov)
Furthermore, nutlin-3a-induced PARP1 degradation enhanced DNA-damaging effects of cisplatin in BRCA1 knockdown cells. (oncotarget.com)
2009). Zinc finger protein 191 (ZNF191/Zfp191) is necessary to maintain neural cells as cycling progenitors. (geneticsmr.com)
It is active in cells and tissues throughout the body, where it interacts with many other proteins. (medlineplus.gov)

This study shows that the expression of both myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) correlates with PKCα expression in TNBC. (oncotarget.com)

Deformed epidermal autoregulatory factor-1 (DEAF-1) is a transcription factor that was originally shown to bind the autoregulatory enhancer of the Deformed ( Dfd ) Hox gene, which is activated in embryonic head segments of Drosophila (Gross, 1996). (sdbonline.org)

Crystal structure of a zinc-finger-RNA complex reveals two modes of molecular recognition. (geneticsmr.com)

Staining of such tissues with TSQ has demonstrated that zinc ions play very important roles in neuronal cell death, endocrine functions, and other physiological phenomena. (dojindo.com)
Poly (ADP-ribose) polymerase 1 (PARP1) plays important roles in single strand DNA repair. (oncotarget.com)

RAB effector protein involved in melanosome transport. (lu.se)

Zinc ion regulates the activities of several enzymes. (dojindo.com)

Stem cell antigen-1 (Sca-1) is a member of the Ly-6 multigene family encoding highly homologous, glycosyl-phosphatidylinositol-anchored membrane proteins. (plos.org)

They have proved somewhat difficult to engineer for new targets, however, because an individual finger may not bind its triplet well in all possible sequence contexts. (ddw-online.com)

First, the general TFs (GTFs), including preinitiation complex components TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and THIIH, are the primary protein factors that are required for the initiation of transcription from the TATA box (or TATA element), then elongation is executed by RNA polymerase II (RNA pol II) [ 1 ]. (intechopen.com)
Here, we used the rat cervical sympathetic trunk (CST), an autonomic nerve in which non-myelinating SCs are the predominant cell type, to isolate zinc-finger protein (ZFP) cDNAs by reverse transcriptase-polymerase chain reaction. (tamu.edu)

Dojindo offers a wide selection of fluorescent intracellular ion probes for calcium, proton, zinc, and chloride ions. (dojindo.com)
Calcium indicators selectively bind to calcium ions. (dojindo.com)
This means that a deprotonation step is involved in the calcium binding process, which causes a change in the fluorescent signal. (dojindo.com)
In designing calcium probes, the BAPTA structure is required as the calcium-binding moiety. (dojindo.com)
If the calcium ion binds to indicators, donation of the lone electron pair on the aromatic nitrogen to the calcium ion causes a change in the fluorescent signaling. (dojindo.com)
Blood lead was significantly higher in the malignant case croups, as were zinc, iron, and calcium. (who.int)

However, the direction of shift perturbation, change of DNA imino-proton NMR signal, and dynamics on the 15 N backbone atom significantly differed between specific and non-specific binding. (sinica.edu.tw)

This gene is located within a cluster of zinc finger family members. (wikipedia.org)
For example, some family members form salt bridges to stabilise the finger-like folds. (embl.de)
Prior research implicated nine members of the zinc-finger transcription factor family in Schwann cell (SC) development and myelination, and levels of one such protein were reported increased in other non-myelinating SCs after denervation. (tamu.edu)
Large number of prokaryotic repressors belong to the helix-turn-helix family of proteins. (vigyansarovar.com)
The protein encoded by this gene belongs to the EGR family of Cys 2 His 2 -type zinc finger proteins. (wikipedia.org)

Blocking the formation of the heterodimer by transfection of MZF-1 60-72 or Elk-1 145-157 peptide fragments at the MZF-1 / Elk-1 interface decreases DNA-binding activity of the MZF-1 / Elk-1 complex at the PKCα promoter. (oncotarget.com)
The albumin promoter is highly active, continuously producing large amounts of albumin protein (approximately 15g/day) which is in excess of the body's requirements. (medicalmvp.com)
DNA constructs were cloned into a pGL3 promoter vector for luciferase gene reporter assays. (cdc.gov)

To gain insights into the interaction mechanism, here we further investigated the DNA-binding characteristics of TZD bound to non-specific DNAs and its finger segments bound to cognate DNA. (sinica.edu.tw)
Using these unique characteristics, we confirmed that the three single-finger segments (TZD 1 , TZD 2 and TZD 3 ) and the two-finger segment (TZD 23 ) non-specifically bind to the cognate DNA. (sinica.edu.tw)
In comparison, the other two-finger segment (TZD 12 ) binding to the cognate DNA features simultaneous non-specific and semi-specific binding, both slowly exchanged in terms of NMR timescale. (sinica.edu.tw)

The first described CID systems involve microbe-derived dimerizing ligands, such as rapamycin, that direct protein neo-associations that rewire eukaryotic cell signaling 5 . (nature.com)
Whereas the polypeptide serving this function in bacteria contains one OB fold, the eukaryotic functional homolog comprises a complex of three proteins, each harboring at least one OB fold. (johnshopkins.edu)

These data suggest that the next therapeutic strategy in treating PKCα-related cancer will be developed from blocking MZF-1/Elk-1 interaction through their binding domain. (oncotarget.com)
RICHMOND, Calif., Dec. 10, 2013 /PRNewswire/ - Sangamo BioSciences, Inc. (Nasdaq: SGMO) announced the presentation of new pre-clinical data demonstrating therapeutic levels of gene modification in non-human primates (NHPs) from its In Vivo Protein Replacement Platform. (medicalmvp.com)
Early data in primates suggest that we can achieve circulating levels of protein above the threshold for therapeutic effect, which we believe are sufficient for the correction of a range of monogenic diseases. (medicalmvp.com)

The Zinc-finger based ATF, and other technology such as CRISPR (clustered regularly interspaced short palindromic repeat) and TALEs (transcription activator-like effectors), are all purified genetically engineered DNA binding proteins that can be designed as specifically as necessary. (cureangelman.org)

A fifth variant (FAM71A rs147978008) showed nonrisk allele preferential binding to H1 histones. (cdc.gov)

Introduction: Duchenne Muscular Dystrophy is a genetic disease that is caused by a deficiency of dystrophin protein. (plos.org)
Recently, we have not only learned to interpret DNA but also edit genetic code. (explorebiotech.com)

DNA recognition in these proteins is provided by modules, each of which binds a single base pair, and there is a robust code to address each of the four base pairs (2,3). (ddw-online.com)
Its major function is in DNA recognition, as α 3 helix is involved in the recognition and is known as " recognization helix " while another one stabilizes the interaction between DNA and Protein. (vigyansarovar.com)

We demonstrate that a direct interaction between the methyl-CpG-dependent transcription repressor Kaiso and xTcf3, a transducer of the Wnt signalling pathway, results in their mutual disengagement from their respective DNA-binding sites. (biologists.com)
In S. cerevisiae , this interaction is at least in part provided by Spp1 that directly interacts with both methylated H3K4 near DSB sites and the axis-localized protein Mer2 (Acquaviva et al. (springer.com)

C) Enlargement of part of the DBD showing the amino acid sequence (single letter codes) of the 2 zinc fingers and the dimerization loop (in bold). (medscape.com)

Anatomy6

Organisms3

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes37

Information Science3

Motifs11

Specificity6

Sequences5

Genes9

Motif8

MRNA4

Different proteins2

Transcription Factors6

Encodes3

Sequence-specific4

Highly conserved3

Nuclease2

Metabolism2

Transcriptional3

Thought to mediate1

Amino acids1

Structures2

Fluorescent Proteins1

Chromatin2

Histone5

Catalytic1

Ligand2

Genome9

Residues3

Interactions1

Single-stranded4

ZFNs3

Genomic2

Domains7

Peripheral nerve conduction1

Affinity2

Kinase3

KRAB2

Mammalian1

Nuclear protein1

Major groove2

Localization1

Humans4

Structural3

Cells5

Myeloid1

19961

Molecular1

Roles2

Effector1

Enzymes1

Multigene1

Targets1

Polymerase2

Calcium6

ATOM1

Family5

Promoter3

Cognate3

Eukaryotic2

Therapeutic3

CRISPR1

Histones1

Genetic2

Recognition2

Interaction2

Dimerization1