Zebrafish muscle development. Medical search. Frequent questions

The role of ESRRs during animal development may also be linked to metabolic regulation by which developing embryos meet their high energy demand for growth. (nature.com)
Our approach is to manipulate fiber-type regulators that function early in development in dmd zebrafish embryos. (seattlechildrens.org)
The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift. (jove.com)
This method can help answer key questions in the developmental biology field because it allows direct observation of tissues and organ development in living embryos over several hours. (jove.com)
The findings could provide new evidences for better understanding the functions of rab11 in the development of zebrafish embryos. (researchsquare.com)
The secondary heart field (SHF) is a conserved developmental domain in avian and mammalian embryos that contributes myocardium and smooth muscle to the cardiac arterial pole. (duke.edu)
In this study, zebrafish embryos were used to evaluate fetal embryo toxicity of the belladonna mother tincture. (ijpsonline.com)
Zebrafish embryos offer a unique opportunity to investigate the mechanisms by which nicotine exposure impacts early vertebrate development. (oregonstate.edu)
We previously demonstrated that secondary spinal motoneurons in nicotine-exposed embryos were delayed in development and that their axons made pathfinding errors (Svoboda, K.R., Vijayaraghaven, S., Tanguay, R.L., 2002. (oregonstate.edu)
The Martin Lab uses zebrafish embryos as a model system. (stonybrook.edu)
The overall goal of the lab is to advance an understanding of the vertebrate body plan via the zebrafish embryos and provide insights to understand stem cell biology and mechanisms of cancer metastasis. (stonybrook.edu)
Martin and colleagues observe zebrafish to demonstrate how differentiated neurons and muscle expand as embryos grow. (stonybrook.edu)
Neuromesodermal progenitors exist in all vertebrate embryos, so zebrafish are used as a common model to these cells' development to better define how the embryonic body plan is formed and how spinal cord and skeletal muscle are induced from this population. (stonybrook.edu)
This image captured by the Martin lab shows three different time points of a developing zebrafish to demonstrate how differentiated neurons and muscle expand as the embryos grow. (stonybrook.edu)
Studies in zebrafish embryos support this hypothesis. (zfin.org)
Several floors down, the team of molecular biologist Christian Mosimann, PhD , is injecting DNA into the embryos of zebrafish. (childrenscolorado.org)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

Gene expression analysis indicates that esrra is expressed in developing pharyngeal arches where genes necessary for cartilage development are also expressed. (nature.com)
Expression of esrra is colocalised with genes necessary for cartilage development in pharyngeal arches during zebrafish embryogenesis. (nature.com)
He studies genes that control positioning of the heart in zebrafish and has found that some of these genes are also critical for normal human heart development through collaborations with clinical geneticists whose patients have family histories of congenital heart defects. (upstate.edu)
We are using CRISPR genome screening and precision editing in zebrafish to engineer mutations in genes that have been implicated in human congenital heart defects. (seattlechildrens.org)
Recently our screening has identified several new genes required for heart development. (seattlechildrens.org)
I describe the temporal and spatial expression patterns of these genes through embryonic development. (rice.edu)
All zebrafish actinin genes have unique expression profiles, indicating specialization of each gene. (rice.edu)
Conclusions: Our results suggest that rab11 genes play important but distinct roles in the development of the nervous system in zebrafish. (researchsquare.com)
In the present project, we showed that zebrafish rab11 genes are conserved in vertebrate evolution by in silicon analysis. (researchsquare.com)
They're vertebrates, we share 70% of the same genes, and 80% of disease-causing genes in humans have a corresponding gene in zebrafish. (chla.org)
In children, cancers may arise from genes that have mutated during development. (chla.org)
What's more, approximately 85% of human genes associated with disease have a counterpart in the zebrafish. (chla.org)
The scientific presentations emphasized developmental processes, encompassing such areas as: 1) the role of homeobox genes and growth factors in inner ear development and plasticity, 2) effects of altered gravitational loading on the development of the inner ear gravity receptor, 3) effects of altered gravitational loading on developing and mature neuromuscular systems and 4) molecular mechanisms that determine muscle fiber diversity and hypertrophic responses. (nih.gov)
Dr. Martin's research expertise includes the use molecular biology techniques such as PCR, mRNA differential display, in situ hybridization, and DNA chip microarrays to identify, clone and characterize genes that are expressed during zebrafish development and whose transcription is regulated by DNA methylation. (sgu.edu)
Dr. Martin's journal review activities include peer reviewed journals Genome, Genes & Development, Transgenic Research, and GENE. (sgu.edu)
During myogenesis, the muscle transcription factor Myogenin plays a pivotal role in orchestrating the expression of other muscle-specific genes. (kcl.ac.uk)
2021. Nitrate-induced improvements in exercise performance are coincident with exuberant changes in metabolic genes and the metabolome in zebrafish skeletal muscle. . (oregonstate.edu)
We found 20 genes that distinguished motor neurons that innervate digit muscles from the others, and there's a strange gene code involved in the cells' development. (scientificamerican.com)
The same genes- RALDH2 and the Hox genes-control motor neuron development, and one intriguing theoretical postulate is that RALDH2 may also be involved in limb development. (scientificamerican.com)
The Crown Research Institute Scion [6] have genetically modified pine trees with the insertion of genes that report the reproductive development of the tree. (sciencemediacentre.co.nz)
The generation of new models will be based on candidate genes identified in WP1 and/or WP2The consortium also aims to use knowledge from genetic and biomarker discovery programmes (in particular, novel pathway information) to support development of novel animal models, and includes European players with a strong track record in this area. (lu.se)
The techniques will include generation of models featuring tissue-specific disruption of selected genes using Cre-loxP systems (in adipocytes, muscle, endothelium, pericytes and myocardium as required). (lu.se)

Though this method can provide insights into spacial and temporal alterations that occur during development of sweeper fish embryo and larvae, it can also be applied to investigate early developmental processes and other model organisms. (jove.com)
We made high-resolution 3D scans of 2-12 days post fertilisation (dpf) zebrafish larvae with fluorescent muscles. (wur.nl)
Some initial findings in larvae of 4 dpf show that muscle fibre size is similar, but that fibre orientation and curvature vary, both along the length of the fish and within a transversal cross section. (wur.nl)
To address this question, the researchers are studying zebrafish larvae. (healthcapital.de)
The researchers used zebrafish larvae whose heart muscle cells produce a fluorescent substance, making it easy to detect them under a microscope. (healthcapital.de)
During early development, the survival of zebrafish larvae GNE-0439 is dependent solely around the innate immune responses because the adaptive immune system is usually functionally mature after 4C6 weeks post fertilization [24]. (mingsheng88.org)
Basnet R ( 2020 ) Peer Review #2 of 'Quantification of the influence of drugs on zebrafish larvae swimming kinematics and energetics (v0.2)' . (peerj.com)

We are investigating the mechanisms of skeletal muscle fiber-type differentiation. (seattlechildrens.org)
A goal of this project is to test a fundamental hypothesis: that factors that promote slow muscle differentiation will ameliorate the effects of DMD. (seattlechildrens.org)
We take advantage of zebrafish models to address whether factors that regulate fiber-type differentiation enhance or suppress the zebrafish dmd muscle degeneration phenotype. (seattlechildrens.org)
Members of this family have numerous roles, including regulation of cell adhesion, cell differentiation, directed cell motility, intracellular signaling and stabilization of f-actin at the sarcomeric Z-line in muscle. (rice.edu)
In zebrafish, Blimp1 promotes differentiation from the embryonic sluggish muscle tissue lineage (9) and specifies neural crest and sensory neuron progenitors (10). (immune-source.com)

Although Rab11a subcellular localization has been demonstrated by transgenic zebrafish [10], and molecular function of Rab11 proteins in primary cilia membrane assembly in Kupffer Vesicle has been studied [6], the developmental expression profile of Rab11 proteins in zebrafish remains elusive. (researchsquare.com)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

A complex expression pattern of ESRRs during animal development seems to be consistent with the potential roles for ESRRa during appropriate developmental programs of tissues and organs in mouse and zebrafish 9 . (nature.com)

The zebrafish is an important and widely used vertebrate model organism in scientific research, for example in drug development, in particular pre-clinical development. (wikipedia.org)
Taken together, our results indicate for the first time that ESRRa is essential for cartilage development by regulating sox9 expression during vertebrate development. (nature.com)
In this report, we used the zebrafish model to examine the role of esrra in cartilage development during vertebrate embryogenesis. (nature.com)

The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family (Cyprinidae) of the order Cypriniformes. (wikipedia.org)
A 4 days post fertilisation zebrafish (Danio rerio) larva. (wur.nl)
Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio). (oregonstate.edu)

Transparency of the embryo and rapid heart development make zebrafish especially appealing to scientists who research the causes of cardiac birth defects. (upstate.edu)
These traits make zebrafish an excellent model for studying human disease. (chla.org)

Duplication of actn3 in the zebrafish indicates that variation in actn3 expression may promote physiological diversity in muscle function among vertebrates. (rice.edu)
Vertebrates use a complex circuit of neurons to coordinate movement and balance-a system that begins with nerve cells in the ears and extends through the spinal cord to activate the muscles that regulate posture and keep the animal upright. (pewtrusts.org)
Zebrafish is a superb model for developmental and toxicological research that may be straight in comparison to various other vertebrates, including mammals. (mingsheng88.org)
As in other vertebrates, the zebrafish anterior segment derives from diverse origins including surface ectoderm, periocular mesenchyme, and neuroepithelium. (biomedcentral.com)
However, several morphogenic features of the zebrafish anterior segment differ with those of higher vertebrates. (biomedcentral.com)

We are also working to identify new epigenetic factors that regulate muscle fiber type. (seattlechildrens.org)
Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. (researchsquare.com)
Among various Rab proteins, Rab11 is known to localize to recycling endosome and regulate vesicular recycling and primary ciliogenesis, playing a key role in Kupffer vesicle development in zebrafish [5-7]. (researchsquare.com)
Some molecular pathways required for SHF development in birds and mammals are conserved in teleosts, as Nkx2.5 and Nkx2.7 as well as Fgf8 regulate Bmp signaling in the zebrafish heart fields. (duke.edu)
Furthermore, several reports claim that Blimp1 might regulate different cellular procedures including cell development or success. (immune-source.com)
These findings indicate that the identified lncRNAs may play essential roles in tissue function and regulate the mechanism of ZBED6 action in skeletal muscle development in pigs. (biomedcentral.com)

We aim to answer this question at the Experimental Zoology Group of Wageningen University, using fluorescence imaging techniques in larval zebrafish. (wur.nl)
For larval fish, the helical pattern was shown to be relatively weak in the anal region, but it is unknown how fibre orientations change along the length of the body and how they adapt these orientations during development. (wur.nl)
This so-called "vestibular" system begins operating early in development and in many different species: Larval zebrafish, for example, can sense when they are lying on their sides and will contract their body muscles to right themselves. (pewtrusts.org)
I will use larval zebrafish as a model system to explore how individual neurons in the vestibular system behave when the animal is tilted in different directions, and how these nerve cells relay this information to the spinal nerves that allow the animal to twist its body to regain an upright posture. (pewtrusts.org)
A transposon-mediated gene trap screen identified the zebrafish line qmc551 that expresses a GFP reporter in primitive erythrocytes and also in haemogenic endothelial cells, which give rise to haematopoietic stem and progenitor cells (HSPCs) that seed sites of larval and adult haematopoiesis. (worktribe.com)

2004). Differential expression of the methyl-cytosine binding protein 2 (MeCP2) gene in embryonic and adult brain of zebrafish . (sgu.edu)
In a paper published in Nature Communications, the authors found that Myogenin is a master regulator of muscle cell fusion, therefore controlling the formation of functional muscle fibres and sustaining normal muscle growth during both embryonic and adult muscle development. (kcl.ac.uk)

Zebrafish represent a powerful model organism for investigating the genetics and cell biology of development and disease. (biomedcentral.com)
scientists with a deep insight into genetics, biomarker discovery, development of novel imaging assays for assessing progression of complications, novel animal models for chronic diabetic complications, in silico models to predict development of complications and response to novel treatments. (lu.se)

To lay the foundation for genetic studies of anterior segment development, we have described the morphogenesis of this structure in zebrafish. (biomedcentral.com)
A detailed understanding of the mechanisms of development of the anterior segment can provide general insights into questions such as tissue induction, cell type fate determination, and the regulation of cellular morphogenesis. (biomedcentral.com)

In this report, we establish an in vivo role of Esrra in cartilage development during embryogenesis in zebrafish. (nature.com)
Vitamin E deficiency dysregulates thiols, amino acids and related molecules during zebrafish embryogenesis. (oregonstate.edu)

Duchenne muscular dystrophy (DMD) is a muscle wasting disease caused by mutations in the DMD gene, which encodes dystrophin. (seattlechildrens.org)
Scientists can study how a gene associated with cancer works in the zebrafish-how mutations in the gene cause cells to become cancerous, and how the cancer cells interact with blood vessels, the immune system and other tissues-and can then apply this information to new treatment studies. (chla.org)
And with CRISPR technology we can then introduce those mutations in zebrafish and see, in great detail, what effect these changes have on cardiovascular development. (childrenscolorado.org)

This image, taken by PhD student Yongchang Ji, shows a cross section of a developing heart in a zebrafish embryo only two days after fertilization. (upstate.edu)
Our study provides new insight into the rab11 expression and promotes the use of this model organism to tackle future studies on the role of rab11 in embryo development. (researchsquare.com)
We have combined zebrafish embryo-based screens with cardiomyogenesis assays to discover selective small molecules that modulate heart development and regeneration with minimal adverse effects. (vanderbilt.edu)

By illustration, a large number of zebrafish and mouse mutants with vestibular system involvement have been developed. (nih.gov)
Even though muscle development is unaffected by nicotine exposure in sop mutants, motoneuron axonal pathfinding errors still occur in these mutants, indicating a direct effect of nicotine exposure on nervous system development. (oregonstate.edu)

In that study, we did not consider the potential role that altered skeletal muscle development caused by nicotine exposure could play in contributing to the errors in spinal motoneuron axon pathfinding. (oregonstate.edu)
In this study, we show that an alteration in skeletal muscle development occurs in tandem with alterations in spinal motoneuron development upon exposure to nicotine. (oregonstate.edu)
The grant enables Martin and colleagues to carry out research designed to more clearly define and observe neuromesodermal progenitors, stem cells that contribute to spinal cord or skeletal muscle development. (stonybrook.edu)
Long noncoding RNAs (lncRNAs) may participate in numerous biological processes, including skeletal muscle development. (biomedcentral.com)
However, the role of the ZBED6-lncRNA axis in skeletal muscle development is poorly characterized. (biomedcentral.com)
Furthermore, by conjoint analysis of longissimus dorsi data (tissue-specific expression, muscle module and DE-lncRNAs) and ChIP-PCR revealed NONSUSG002145.1 (adjusted p -values = 0.044), which is coexpressed with the IGF2 gene and binding with ZBED6, may play important roles in ZBED6 KO pig skeletal muscle development. (biomedcentral.com)
These results may open new research directions leading to a better understanding of the global functions of ZBED6 and of lncRNA functions in skeletal muscle development in pigs. (biomedcentral.com)

We then analyzed the temporal and spatial expression of rab11a gene in embryonic development by RT-PCR and whole mount in situ hybridization. (researchsquare.com)

However, direct evidence regarding its involvement in cartilage development remains elusive. (nature.com)

In one research line, we measure muscle activation patterns in fish muscles labelled with voltage sensitive fluorescent proteins. (wur.nl)
In a second research line, on which we focus here, we study the 3D architecture of axial muscle fibres labelled with Green Fluorescent Protein. (wur.nl)
Her pictures of these technicolor brains, which show individual nerve cells aglow in a spectrum of fluorescent colors, can help explain how neural connections form in zebrafish-uncovering principles likely to be directly relevant to the human brain. (lclark.edu)

Apart from brain, rab11a was also expressed in caudal vein, pronephric duct, proctodeum, pharyngeal arches and digestive duct, rab11ba was detected to express in muscle, and rab11bb was expressed in kidney, fin and spinal cord. (researchsquare.com)
Nicotinic receptors mediate changes in spinal motoneuron development and axonal pathfinding in embryonic zebrafish exposed to nicotine. (oregonstate.edu)

This work will eventually help us understand why certain types of muscle fibers are more susceptible to muscular dystrophy. (seattlechildrens.org)
Certain muscular dystrophies preferentially affect either fast or slow twitch muscle fibers. (seattlechildrens.org)
In human and mouse models of Duchenne Muscular Dystrophy (DMD), fast muscle fibers are more susceptible to damage than slow fibers. (seattlechildrens.org)
As a result, we were better able to observe elusive features in several different biological samples, such as retinal structures in the zebrafish eye and muscle fibers in mouse esophagus. (photonics.com)
Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
And how do they generate long-distance fibers that stretch out to the digit muscles as opposed to short ones that are going to innervate, say, a shoulder muscle? (scientificamerican.com)
and (uncommonly) necrotic muscle fibers. (medscape.com)

We are screening epigenetic chemicals for their abilities to enhance or suppress the zebrafish dmd phenotype. (seattlechildrens.org)
Muscle-specific ablation of Pik3c2b , but not Pik3c3 , resulted in complete prevention of the MTM phenotype, and postsymptomatic targeting promoted a striking rescue of disease. (jci.org)
We confirmed this genetic interaction in zebrafish, and additionally showed that certain PI3K inhibitors prevented development of the zebrafish mtm phenotype. (jci.org)

Early heart formation in zebrafish is remarkably similar to what takes place in humans. (upstate.edu)
Despite its lack of septation, the tissue patterning of the arterial pole of the zebrafish is remarkably similar to the patterning of pulmonary and aortic arterial poles observed in mouse and chick. (duke.edu)

One focus of our lab is understanding the mechanisms behind how skeletal muscle cells differentiate into a specific muscle fiber type, either fast twitch or slow twitch. (seattlechildrens.org)
Our long-term goal with this project is to achieve a precise understanding of the genetic and transcriptional mechanisms underlying early heart development. (seattlechildrens.org)
Cumulatively, our anatomical findings provide a reference point to utilize zebrafish for genetic studies into the mechanisms of development and maintenance of the anterior segment. (biomedcentral.com)
Mechanisms of Development. (zfin.org)

For example, we have applied light-activatable cMOs to interrogate transcription factor function during zebrafish notochord, pancreas, and vascular patterning. (cdc.gov)

The transposon that mediates this GFP expression is located in intron 1 of the gfi1aa gene, one of three zebrafish paralogs that encode transcriptional repressors homologous to mammalian Gfi1 and Gfi1b proteins. (worktribe.com)

Additionally, the transcription factor Tbx1 and the Sonic hedgehog pathway are necessary for normal development of the zebrafish arterial pole. (duke.edu)
Achn expression is induced in myoblasts that form myotubes and acts upstream of the muscle specific transcription factor MyoD. (zfin.org)

Dr. Amatruda, who holds the Dr. Kenneth O. Williams Chair in Cancer Research, studies solid tumor cancers in children, including liver and kidney cancers and sarcomas (cancers of the bone, muscle and connective tissue). (chla.org)
A heart attack occurs when blood vessels that supply blood and oxygen to the heart muscle become blocked, causing part of the heart muscle tissue to die. (healthcapital.de)
Instead, connective tissue cells known as fibroblasts migrate into the damaged area of the heart muscle. (healthcapital.de)
These signals also played an important role in regenerating heart muscle tissue. (healthcapital.de)
This will help us understand why heart muscle tissue is unable to regenerate in humans. (healthcapital.de)
The team also hopes to identify potential targets for influencing the interaction between the nervous and immune systems in a way that promotes the regeneration of heart muscle tissue and the maintenance of heart function in heart attack patients. (healthcapital.de)
Whales today don't have digit muscles, but some fishes gradually acquired embryonic digit-forming tissue in their fin muscles. (scientificamerican.com)
CDMG treatment of amputated zebrafish hearts reduces nuclear β-catenin in injured heart tissue, increases cardiomyocyte (CM) proliferation, and expedites wound healing, thus accelerating cardiac muscle regeneration. (vanderbilt.edu)

Recent studies indicate that members of ESRRs participate in a number of biological processes including metabolism, reproduction and development 4 . (nature.com)

So it stands to reason that the communication between the autonomic nervous and immune systems determines whether heart muscle scarring will occur or whether the heart muscle can recover. (healthcapital.de)
The nicotine-induced alteration in muscle development does not occur in the zebrafish mutant (sofa potato, [sop]), which lacks muscle-specific AChRs. (oregonstate.edu)

The human heart shows limited natural muscle regeneration in response to myocardial infarction. (fondationleducq.org)

These findings could inform the development of devices that can help people regain their balance and posture, abilities that can be impaired by injury or disease. (pewtrusts.org)
Their findings also revealed that another molecule, named Hedgehog, promotes the formation of multinucleated muscle fibres via a second, Myogenin-independent, pathway. (kcl.ac.uk)
The authors hope these findings will allow them to make progress in understanding how the correct number of muscle fibres with the right sizes are formed in the body. (kcl.ac.uk)
The diagnosis of congenital muscular dystrophy is now based on clinical findings, muscle biopsy results, and genetic information. (medscape.com)

In the next step, the researchers engineered genetically modified zebrafish in which the adrenergic signal reached the macrophages but could not be transmitted from the receptor into the cell's interior. (healthcapital.de)

Evolution & Development (2023) 25 (3): 197. (biologists.com)

We are using a zebrafish model of Duchenne muscular dystrophy (the dmd mutant strain) to investigate the developmental biology and epigenetics of DMD as well as to identify new drug therapies for DMD. (seattlechildrens.org)
Measurements at different developmental stages will show us how the fish restructure their swimming muscles while growing. (wur.nl)
Digit-innervating motor neurons also lack the RALDH2 gene, which encodes an enzyme that synthesizes a key developmental biochemical called retinoic acid, and is critical to the development of all other types of motor neurons. (scientificamerican.com)

The 3,000-square foot lab will support scientists studying many pediatric conditions and house up to 120,000 zebrafish. (chla.org)

We use the zebrafish as an animal model because of advantages for genetic manipulations, in vivo imaging and drug screening. (seattlechildrens.org)
We expect that this project will identify genetic and epigenetic regulators of muscle fiber-type identities that confer susceptibility or resistance to muscular dystrophy. (seattlechildrens.org)
The development of caged MOs (cMOs) has significantly extended the versatility of these reverse-genetic tools. (cdc.gov)

Although the existence of additional anomalies of the nervous system or other organ systems is difficult to exclude, the existing animal models are a valuable resource for understanding molecular bases for normal inner ear development. (nih.gov)
Researchers from the Randall Centre for Cell & Molecular Biophysics believe they have reached a new milestone in the understanding of muscle formation that could help define new cellular and molecular approaches in pathological conditions affecting muscle wellness. (kcl.ac.uk)
Identifying the molecular basis of sexual dominance in male zebrafish. (exeter.ac.uk)
However, the relevant cellular interactions between various structures of the anterior segment and the molecular basis of development is just beginning to be understood. (biomedcentral.com)

The team found that a cancer-causing "fusion oncogene" called PAX3-FOXO1 disrupts muscle development in children, causing these cells to keep growing instead of maturing. (chla.org)
Using the zebrafish, his team found a specific gene that disrupts muscle development, locking cells into rapid division. (chla.org)

Weissman-Unni's neurobiology training focused on mammalian systems, but she chose to shift her independent research program to zebrafish because they are one of the most powerful model systems for imaging the developing brain in living organisms. (lclark.edu)

We recently identified novel epigenetic small molecules that improve muscle degeneration in DMD zebrafish ( https://doi.org/10.1186/s13395-020-00251-4 ). (seattlechildrens.org)
Uncoupling nicotine mediated motoneuron axonal pathfinding errors and muscle degeneration in zebrafish. (oregonstate.edu)

Vitamin E is necessary for zebrafish nervous system development. (oregonstate.edu)

To grow large and strong, each fibre is formed by the fusion of several muscle precursor cells in a process called myogenesis. (kcl.ac.uk)

Our research includes the use of knockout mice and zebrafish as laboratory models. (ncl.ac.uk)
IGF2 mutation knock-in (IGF2 KI) and ZBED6 knockout (ZBED6 KO) lead to changes in IGF2 expression and increase muscle mass in mice and pigs. (biomedcentral.com)
Muscle fiber size was analyzed in developing (P9) mice lacking SPARC, Hevin, and both SPARC and Hevin. (bvsalud.org)
NMJ morphology was assessed in developing (P9), adult (P90) and injured (fibular nerve crush) mice lacking SPARC, Hevin, and both SPARC and Hevin skeletal muscle. (bvsalud.org)
Hevin-/- mice exhibited delayed NMJ and muscle fiber development but displayed normal NMJ morphology in adulthood and accelerated NMJ reinnervation following nerve injury. (bvsalud.org)
Mice lacking SPARC displayed normal NMJ and muscle fiber development but exhibited smaller NMJs with fewer acetylcholine receptor islands in adulthood. (bvsalud.org)

The generation of mutant and transgenic animal models, involving such species as the mouse, Drosophila, zebrafish and Caenorhabditis elegans (C. elegans), provides powerful tools for elucidation of these functions. (nih.gov)
To gain insight into Myogenin function the team used the zebrafish as an animal model and created a mutant in which the expression of Myogenin protein was abolished. (kcl.ac.uk)

This is, roughly speaking, the same reason Tamily Weissman-Unni makes diagrams and maps of neural circuitry within the humble 1-inch-long zebrafish, whose small brains she lights up like the brilliant rainbows that occasionally appear in the skies above Portland when the sun peeks through the rain. (lclark.edu)

2002). Expression and genomic organization of the zebrafish chaperonin gene complex . (sgu.edu)
A Comprehensive, high-resolution genomic transcript map of human skeletal muscle. (unipd.it)

Each muscle is composed of bundles of individual muscle fibres capable of physical contraction. (kcl.ac.uk)

This zebrafish SHF contributes myocardium after initial heart tube formation, giving rise to both smooth muscle and myocardium. (duke.edu)
2004). Histone deacetylase 1 (HDAC-1) required for normal formation of cartilage and pectoral fins of the zebrafish . (sgu.edu)
An in vivo study of signaling pathways involved in zebrafish thyroid formation. (unipd.it)
Reduction of Achn with antisense morpholinos led to muscle fiber loss and an increase in the number of surviving cells in the somites, while ectopic Achn enhanced muscle fiber formation and reduced cell numbers. (zfin.org)

However, aside from muscle development, the roles of ESRRs in other tissues including bone and cartilage have just begun to be investigated 10 . (nature.com)
In this study, we assembled transcriptomes using RNA-seq data published in previous studies by our group and identified 11,408 known lncRNAs and 2269 potential lncRNAs in seven tissues, heart, longissimus dorsi, gastrocnemius muscle, liver, spleen, lung and kidney, of ZBED6 KO (lean mass model) and WT Bama pigs. (biomedcentral.com)
Weighted gene coexpression network analysis (WGCNA) of 1570 DE-lncRNAs between WT and ZBED6 KO pigs was used to define the following six lncRNA modules specific to different tissues: skeletal muscle, heart, lung, spleen, kidney and liver modules. (biomedcentral.com)

The zebrafish is native to freshwater habitats in South Asia where it is found in India, Pakistan, Bangladesh, Nepal and Bhutan. (wikipedia.org)
Enter the zebrafish, a freshwater member of the minnow family. (chla.org)

Normally, a child's primitive fetal cells grow and mature into organs, bones and muscles. (chla.org)
Over just a few weeks, researchers can track a tumor's development, gaining insight into how normal cells in the tumor's "neighborhood" are coerced into promoting the tumor's growth. (chla.org)
In normal development, cells divide rapidly until an organ forms. (chla.org)
This damage is permanent because the human heart has no ability to grow new heart muscle cells. (healthcapital.de)
The role of DNA methylation on sexual determination and development in zebrafish germ cells. (exeter.ac.uk)
One new promising line of research focuses on expanding the heart's limited ability to produce new muscle cells. (fondationleducq.org)
During development, cells from diverse embryonic lineages interact to form the anterior segment. (biomedcentral.com)
Dr. Nakano first isolates the individual heart muscle cells. (childrenscolorado.org)

Zebrafish are also closely related to the genus Devario, as demonstrated by a phylogenetic tree of close species. (wikipedia.org)
The unusually cold temperature was at one of the highest known zebrafish locations at 1,576 m (5,171 ft) above sea level, although the species has been recorded to 1,795 m (5,889 ft). (wikipedia.org)

Anatomy58

Organisms19

Diseases10

Chemicals and Drugs51

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes52

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Embryos17

Genes22

Larvae7

Differentiation5

Transgenic zebrafish2

Mouse and zebrafish1

Vertebrate3

Danio3

Make zebrafish2

Vertebrates5

Regulate6

Larval5

Embryonic and adult2

Genetics2

Morphogenesis2

Embryogenesis2

Mutations3

Embryo3

Mutants2

Skeletal muscle development7

Gene in embryonic1

Cartilage development1

Fluorescent3

Spinal2

Fibers7

Phenotype3

Remarkably similar2

Mechanisms4

Notochord1

Paralogs1

Transcription factor2

Tissue8

Biological1

Occur2

Regeneration1

Findings4

Genetically1

20231

Developmental3

Scientists1

Genetic3

Molecular4

Disrupts2

Mammalian1

Degeneration2

Nervous system development1

Myogenesis1

Mice6

Mutant2

Neural1

Genomic2

Individual muscle fibres1

Formation4

Tissues3

Freshwater2

Cells8

Species2