• WNT1-inducible-signaling pathway protein 2, or WISP-2 (also named CCN5) is a matricellular protein that in humans is encoded by the WISP2 gene. (wikipedia.org)
  • Wnt-1 inducible signaling pathway protein 2 (WISP-2/CCN5) is a recently identified adipokine that has been described as an important mediator of canonical Wnt activation in adipogenic precursor cells. (harvard.edu)
  • Hepatic Wnt1 Inducible Signaling Pathway Protein 1 (WISP-1/CCN4) Associates with Markers of Liver Fibrosis in Severe Obesity. (mpg.de)
  • Recombinant Human WNT1-inducible-signaling pathway protein 1 encodes amino acid residues 23-367. (reprokine.com)
  • The CCN acronym is derived from the first three members of the family identified, namely CYR61 (CCN1), CTGF (connective tissue growth factor, or CCN2), and NOV. These proteins, together with WISP1/CCN4, WISP2 (CCN5, this gene), and WISP3 (CCN6) comprise the six-member CCN family in vertebrates. (wikipedia.org)
  • WISP-2 (CCN5) inhibits the proliferation of vascular smooth muscle cells, human uterine myometrial cells, and leiomyoma cells. (wikipedia.org)
  • There are three other family members WISP-1, WISP-2 and WISP-3, which are designated as CCN4, CCN5 and CCN6 (Brigstock, 2003). (abic2004.org)
  • This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. (utsouthwestern.edu)
  • This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. (utsouthwestern.edu)
  • The CCN family presently consists of six members in human also known as: Cyr61 (Cystein rich 61), CTGF (Connective Tissue Growth Factor), Nov (Nephroblastoma Overexpressed gene), WISP-1, 2 and 3 (Wnt-1 Induced Secreted Proteins). (neuromics.com)
  • Gene silencing of WISP-2 increased the induction of the catabolic markers MMP-13 and ADAMTS-5 and the inflammatory mediators IL-6 and IL-8 triggered by IL-1β in human primary OA chondrocytes in a Wnt/β-catenin dependent manner. (harvard.edu)
  • The CCN6 gene provides instructions for making a protein that appears to be involved in bone growth and the maintenance of cartilage, which covers and protects the ends of bones. (medlineplus.gov)
  • The tumor also contained a novel t(2;22)(q34;q12) translocation involving the EWSR1 gene, which is consistent with additional reports suggesting that a growing list of translocations can drive formation of, and potential new management strategies for, EMC. (cancer-genetics.org)
  • Incubation of HGC27 WISP-2 kd cells with both 0.75?small inhibitors and 1.5?with WISP2 tumour/normal ratio are an interesting observation. (abic2004.org)
  • Mechanistically, WISP2 exerts its tumor suppressive functions via regulation of ERK1/2, Slug, and E-cadherin in ESCC cells. (biomedcentral.com)
  • WISP-2 is a member of the CCN family (CCN intercellular signaling protein) of secreted, extracellular matrix (ECM)-associated signaling matricellular proteins. (wikipedia.org)
  • CCN proteins are matricellular proteins which are involved in the regulation of various cellular functions including: proliferation, differentiation, survival, adhesion and migration. (neuromics.com)
  • The CCN genes encode secreted proteins associated with the Extracellular Matrix (ECM) and cell membrane. (neuromics.com)
  • In conclusion, here we have shown for the first time that WISP-2 may have relevant roles in modulating the turnover of extracellular matrix in the cartilage and that its downregulation may detrimentally alter the inflammatory environment in OA cartilage. (harvard.edu)
  • Full length secreted CCN proteins can show an antiproliferative activity, whereas truncated isoforms are likely to stimulate proliferation and behave as oncogenes. (neuromics.com)
  • Kd of WISP-2 expression promoted the proliferation, migration and invasion of GC cells. (abic2004.org)
  • WNT, fibroblast growth factor (FGF), Notch, Hedgehog, and transforming growth factor β/bone morphogenetic protein signaling network are implicated in the maintenance of tissue homeostasis by regulating self-renewal of normal stem cells as well as proliferation or differentiation of progenitor (transit-amplifying) cells. (aacrjournals.org)
  • We demonstrated that recombinant human WISP-2 protein reduced IL-1β-mediated chondrocyte catabolism, that IL-1β and WNT/b-catenin signaling pathways are involved in rhWISP-2 protein and IL-1β effects in human chondrocytes, and that WISP-2 has a regulatory role in attenuating the catabolic effects of IL-1β in chondrocytes. (harvard.edu)
  • Recombinant WISP1 migrates as an approximately 38 kDa protein under reducing conditions in SDS-PAGE. (reprokine.com)
  • WISP-2 expression is modulated along chondrocyte differentiation and downregulated at the onset of hypertrophy by inflammatory mediators. (harvard.edu)
  • Positive WISP-2 protein staining in GC are associated with a longer survival of the patients, and with differentiation of GC cells in gastric tumour, namely, 23 out of 37 (62%) positively stained in well/moderate differentiated tumours 58 out of 140 (41%) in poorly differentiated tumours. (abic2004.org)
  • Proteolysis of the secreted full-length CCN proteins that has been reported in the case of CCN2 and CCN3 might result in the production of CCN-derived peptides with high affinity for ligands that full-length CNN proteins bind only poorly. (neuromics.com)
  • Ectopic expression of WISP-2 also inhibits the motility and invasiveness of breast carcinoma cells. (wikipedia.org)
  • Cell motility analysis indicated that WISP-2 kd contributed to GC cells' motility and can be attenuated by PLC-and JNK small inhibitors. (abic2004.org)
  • Although recent evidence suggests a role for Wnt signaling in OA physiopathology, little is known about the involvement of WISP-2 in cartilage degradation. (harvard.edu)
  • We also investigated the effect of WISP-2 on cartilage catabolism and performed WISP-2 loss-of-function experiments using RNA interference technology in human T/C-28a2 immortalized chondrocytes. (harvard.edu)
  • It is part of a family of proteins that are involved in the growth and maintenance of connective tissues, such as bone, cartilage, and blood vessels. (medlineplus.gov)
  • The CCN6 protein is made in cells called chondrocytes, which produce and maintain cartilage, and is associated with the production of certain proteins that make up cartilage, but its role in their production is unclear. (medlineplus.gov)
  • Loss of CCN6 protein function likely disrupts normal cartilage maintenance and bone growth, leading to the joint problems in PPRD. (medlineplus.gov)
  • The function of the CCN6 protein is not well understood. (medlineplus.gov)
  • Most of the mutations involved in this condition lead to production of an abnormally short CCN6 protein that is probably nonfunctional. (medlineplus.gov)
  • 1.12?inhibitor STK870702, the migration distance of HGC27 WISP-2 kd cells (Resistance) decreased significantly as the concentration increased from 0.112 to 1 1.125?inhibitor did not change the migration distance of pEF cells significantly at any concentrations (*(B) and JNK (C) pathways. (abic2004.org)
  • Conclusions: Increased expression of WISP-2 in GC is positively correlated with favourable clinical features and the survival of patients with GC and is a negative regulator of growth, migration and invasion in GC cells. (abic2004.org)
  • CCN proteins characteristically contain an N-terminal secretory signal peptide followed by four structurally distinct domains with homologies to insulin-like growth factor binding protein (IGFBP), von Willebrand type C repeats (vWC), thrombospondin type 1 repeat (TSR), and a cysteine knot motif within the C-terminal (CT) domain. (wikipedia.org)
  • Wnt-1 is a secretory protein to conduct signal transduction necessary for embryogenesis via a cell surface receptor, the Frizzeled family. (fujifilm.com)
  • WISP-2 expression was inversely correlated with that of Twist and Slug in paired samples. (abic2004.org)
  • WISP-2 suppressed GC cell metastasis through reversing EMT and suppressing the expression and activity of MMP9 and MMP2 via JNK and ERK. (abic2004.org)
  • This study has provided new data Difloxacin HCl that expression of WISP-2 at mRNA and protein levels are also aberrant in GC. (abic2004.org)
  • The expression of ERK-1/2, Slug and E-cadherin was measured by Western blot respectively. (biomedcentral.com)
  • Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. (utsouthwestern.edu)
  • WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. (utsouthwestern.edu)
  • failure of the signals or of the B cells to adapt adequately in relation to insulin sensitivity results in inappropriate insulin levels, impaired fasting glucose (IFG), impaired glucose tolerance (IGT), and type 2 diabetes. (medscape.com)
  • It has been shown that WISP proteins are upregulated in Wnt-1-transformed cells (Pennica studies suggest loss of WISP-2 signalling may be a crucial permissive event for EMT and ECM degradation and cell migration. (abic2004.org)
  • Noncanonical WNT signals are transduced through Frizzled family receptors and ROR2/RYK coreceptors to the Dishevelled-dependent (Rho family GTPases and c-jun NH 2 -terminal kinase) or the Ca 2+ -dependent (NLK and nuclear factor of activated T cells) signaling cascades. (aacrjournals.org)
  • Other mutations change single protein building blocks (amino acids) in the protein. (medlineplus.gov)
  • It is 72% identical to the mouse protein at the amino acid level. (utsouthwestern.edu)
  • It is the leasts conserved one of the four domains at the level of nucleotide sequence, but it appears to be critical for several of the biological functions attributed to the CCN proteins. (neuromics.com)
  • Davis L, Chen Y, Sen M. WISP-3 functions as a ligand and promotes superoxide dismutase activity. (medlineplus.gov)
  • WISP-2 also inhibits cardiac hypertrophy and fibrosis, an effect that appears linked to the absence of the CT domain. (wikipedia.org)
  • Moreover, WISP overexpression retarded tumor growth in mouse model. (biomedcentral.com)
  • However, WISP-2 is unique among this family of proteins by lacking precisely the CT domain. (wikipedia.org)
  • Module IV resembles the CT domain of several extracellular protein including, Von Willebrand's factor and mucins. (neuromics.com)
  • The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. (utsouthwestern.edu)
  • The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. (reprokine.com)
  • Thus, targeting WISP-2 might represent a potential therapeutical approach for degenerative and/or inflammatory diseases of musculoskeletal system, such as osteoarthritis. (harvard.edu)
  • These findings suggest that WISP-2 is a potential tumour suppressor in GC. (abic2004.org)
  • Go to Diabetes Mellitus, Type 1 and Diabetes Mellitus, Type 2 for complete information on these topics. (medscape.com)
  • Thus, in line with the reports in breast cancer, the current study would support the hypothesis that WISP-2 is a candidate biomarker. (abic2004.org)