Vitro cell protocols cells cultured. Medical search. Frequent questions

Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
This co-culture system allows investigation into the effects of myocyte derived soluble factors uponepicardial EMT and mesenchymal cell differentiation. (arizona.edu)
However, it increases proliferation rates in some cell types (e.g. mesenchymal stem cells, osteosarcoma cells, HUVECs) which puts extra demands on nutrient supply and waste removal [1,2,3] . (reprocell.com)
In vitro expansion of mesenchymal stem cells using 3-D matrix derived from cardiac fibroblast. (reprocell.com)
This study tested the hypothesis that double overexpression of miR-19a & miR-20a (dOex-mIRs) in human induced pluripotent stem cell (iPS)-derived mesenchymal stem cells (MSCs) effectively preserved left-ventricular-ejection-fraction (LVEF) in dilated cardiomyopathy (DCM) (i.e., induced by doxorubicin) rat. (researchsquare.com)
11. Kramer PR, Nares S, Kramer SF, Grogan D, Kaiser M. Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro. (bvsalud.org)

The release of IL-8 was quantified in cell culture media using the enzyme-linked immunosorbent assay (ELISA). (cosmeticsandtoiletries.com)
For example, after 1 hr exposures to 0.5 μ/ml, surviving fractions determined by colony formation assay were approximately 0.3 for cells from spheroids treated intact and 0.001 for single exponential phase cells. (researchgate.net)
Assay conditions, such as culture format and duration, media supplements etc., were reported previously to alter the phenotype and induction response of cultured hepatocytes ( LeCluyse, 2001 ). (aspetjournals.org)
Engineered NK cells had an enhanced ability to mediate ADCC as measured both via NK cell degranulation (CD107a expression, Figure 2A) and specific lysis of B cell lymphoma cells ( 51 Cr release assay, Figure 2B). (maxcyte.com)
The aim of this study was three-fold, firstly to identify new regulators of tendon development and to analyze their expression in Meox2 mutants, secondly, to analyze the intrinsic regulation of myogenic differentiation by Meox2 transcription factor, and thirdly to establish an in vitro assay to analyze tendon fibroblast-myoblast interactions. (confex.com)
function of proliferative responses provoked in the induction cell products favor immediate type hypersensitivity reactions, phase of contact allergy, the murine local lymph node assay serving as growth and differentiation factors for mast cells and (LLNA), has been accepted as a stand-alone method for skin eosinophils, the cellular effectors of the clinical manifestations of sensitization testing (NIH, 1999). (cdc.gov)

Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq , " Evaluation of Cellular Processes by In Vitro Assays ", Bentham Science Publishers (2018). (benthamscience.com)
Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. (benthamscience.com)
To determine how the ASAP complex interacts with the EJC core, we performed in vitro coprecipitation assays 3 with Tandem Affinity Purification (TAP)-tagged recombinant proteins corresponding to full-length human SAP18, RNPS1 and ACINUS. (nature.com)

The potential to generate functional pancreatic beta cells from human embryonic stem cells provides a promising avenue for beta cell replacement therapy for treatment of diabetes mellitus. (ca.gov)
In our lab, we found that hundreds of genes enriched in functional beta cells were not properly induced in the nonfunctional cells derived from embryonic stem cells, suggesting that manipulating critical regulators that can affect multiple beta cell genes simultaneously might be instrumental in directing beta cell differentiation and maturation in the culture dish. (ca.gov)
They can be derived directly from isolated organs or differentiated from embryonic stem cells or induced pluripotent stem cells. (springeropen.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

We found that the expanded cells possessed significantly improved immune effector functions, including proliferation, differentiation, and cancer cell killing, both in vitro and in the humanized mouse model. (nature.com)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
Cytosine arabinoside (AraC) 10 μ M was added to the media the last 2 days to prevent proliferation of other glial cells. (hindawi.com)
The introduction of cell culture techniques has helped a lot in understanding the physiological processes like cell signalling, neurobiology, cell proliferation, pathogenesis of diseases, apoptosis and even more. (benthamscience.com)
regulating the differentiation, proliferation, maintenance and sculpturing organs and tissues, functioning of immune system and the elimination of defective harmful cells. (benthamscience.com)
The systems have been shown to improve cell proliferation and specific protein expression beyond that seen for static 3D cultures [4,5,6,7] . (reprocell.com)
The levels of glucose and secreted lactate contained in the cell culture medium were monitored as indicators of metabolic depletion, while protein syntheses and cell proliferation were measured by expression of phosphorylated Ribosomal protein S6 and cyclin D1. (reprocell.com)
Results suggested that perfusion culture reduces hypoglycemia, anaerobic stress, and preserves protein synthesis and cell proliferation. (reprocell.com)
Once a tissue sample is received, it is enzymatically digested, directly embedded into a 3D environment, and fed with growth factor and Wnt ligand-rich media that stimulates cancer cell proliferation and organoid formation. (corning.com)
This loss in the glucose stimulated insulin secretion (GSIS) phenotype correlates with changes in morphology, increased proliferation and increased alkaline phosphatase (AP) activity, suggesting that the loss of GSIS may be due to de-differentiation of the high passage MIN6 cells. (dcu.ie)
On the other hand, for primary teeth, enzymatic digestion always promoted a higher cell proliferation. (bvsalud.org)

The loss of pancreatic beta-cells in type 1 diabetes results in absence of insulin secreted by the pancreas, and consequently elevated blood sugar which leads to various long-term complications. (ca.gov)
In a separate project, scientists from the University of Oxford cultured the human pancreatic cancer cell line PSN-1 over a period of seven days, in either static or perfused conditions, using the Alvetex perfusion plate . (reprocell.com)
In this article, Dr. Hervé Tiriac, associate project scientist at the University of California (UC), San Diego, discusses his role in creating a pancreatic organoid lab for testing cancer therapeutics in vitro . (corning.com)
Dr. Hervé Tiriac has dedicated his career to fine-tuning the protocols for establishing pancreatic organoids. (corning.com)
The murine embryonic stem (ES) cell line, ES-D3 was used in this project as a model for (a) investigating controversial aspects of current protocols used for the derivation of (3 cell types from ES cells and (b) investigating the potential to regulate the differentiation of ES cells into endodermal derivatives 1 e 0-1 ike cells, using extra-cellular factors previously associated with various aspects of pancreatic development. (dcu.ie)
Treatment of these aggregates with retinoic acid (RA) was previously shown to induce endodermal differentiation We exposed RA_EBs to secondary treatment with agents associated with pancreatic endocrine differentiation, e g Activin A (AA), Sodium Butyrate (SB) and Betacellulin (BTC), with the aim of developing a novel protocol for the regulated derivation of insulin-expressing cell types. (dcu.ie)
Applying 2-stage regulated differentiation protocols developedduring the course of the study, we demonstrated the derivation of an intermediate multi-potential population (RAJEBs) from undifferentiated ES cells that preferentially gave rise to pancreatic endocrine insulin-expressing cell types in the presence of SB and neuronal-like cell types in the presence of AA or BTC. (dcu.ie)

In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
July 20, 2021 -- Cellular reprogramming of ex vivo cells may offer improvements on first-generation adoptive cell therapies, which have experienced limited clinical success due to complex manufacturing, potential patient rejection, and availability of clinical cell types. (scienceboard.net)
The clinical success of anti-CD19 CAR T-cell therapies has not only provided efficacious treatment alternatives for cancer patients but also aided the developmental and regulatory precedent for the translation of cell-based therapies for other disease areas. (scienceboard.net)
Developing next-generation cell therapies centers around the enhancement of efficacy, safety, and accessibility. (scienceboard.net)
Prior to the invention of CAR, cell therapies were largely limited to allogeneic hematological transfusion and transplantation. (scienceboard.net)
First-generation ex vivo cell therapies, such as autologous anti-CD19 CAR T cells, demonstrated the clinical value and curative potential of enhancing the therapeutic qualities of immune and other patient-derived cell types. (scienceboard.net)
Only 10% of the human population is homozygous for this polymorphism, suggesting that genetic manipulation of NK cells to express CD16-158V prior to adoptive transfer may improve clinical success not only of NK cell therapies but also anti-tumor IgG1 antibodies. (maxcyte.com)
Through his extensive research, Tiriac also reports that, if a patient's organoids are sensitive to therapies in vitro , then the patient tends to be sensitive to the same drug given in the clinic. (corning.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
In Papers I and Paper III of my thesis, we examined stem cell-derived therapies' potential on two most common conditions of xerostomia, which are acute irradiated and chronic immune dysregulation-caused salivary gland hypofunction. (lu.se)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)

A subset of epicardial cells undergoes EMT, migrates into the developing heart, and differentiates into cardiac fibroblast, vascular endothelial, and smooth muscle cells. (arizona.edu)
To label myoblasts for use in the tendon fibroblast-myoblast co-cultures, we investigated the potential use of a variety of fluorescently labeled cellular reporters, such as: Golgi-GFP, nuclear histoneH2B-Cherry, and membrane localized CAAX-Cherry. (confex.com)

3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)
Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Since the mammalian cells do not uptake the foreign DNA efficiently, the availability of effective methods for introducing genes into the cells is essential. (benthamscience.com)
In order to more effectively study epicardial EMT in vitro, we developed several new methods for the in vitro study of coronary vessel development. (arizona.edu)
The assessment of potential drug-drug interactions by in vitro methods has become an indispensable part of the preclinical development process of new drug candidates. (aspetjournals.org)
Methods: Fluorescence microscopy confirmed that mTHPC was incorporated into all three cell types. (open.ac.uk)
This study was developed to compare the morphological, proliferative and immunophenotypic profiles of pulp cells from permanent and primary teeth, obtained by two isolation methods. (bvsalud.org)
To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)

Along with Geeta K. Vemuganti, an ophthalmologist with interest in limbal stem cell studies, he carried out research on the subject and together they developed a methodology for growing stem cells for subsequent transplantation in human eyes for repairing injured epithelium and cornea. (wikipedia.org)
Bioinks can be 3D bioprinted into functional tissue constructs for drug screening, disease modeling, and in vitro transplantation. (sigmaaldrich.com)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)

We propose to manipulate these candidate regulators in vitro in order to force beta-cell precursors to adopt a mature phenotype. (ca.gov)
Indeed, a number of genes identified as potential manipulators of the host cell phenotype are absent from the T . equi genome. (biomedcentral.com)
Specifically, demonstrate efficient expression of high-affinity CD16 in NK cells with minimal effects on cell viability and phenotype using mRNA electroporation and assess the effects of CD16-158V expression on rituximab - mediated cytotoxic activity against B cell lymphoma cells. (maxcyte.com)
Importantly, no major changes in NK cell phenotype, as measured by expression of 15 inhibitory or activating surface receptors, were observed following electroporation with CD16-158V mRNA. (maxcyte.com)

We present you 3 practical solutions on how to improve your 3D spheroid in vitro experiments. (researchgate.net)

3 H]Flunitrazepam and [ 3 H]TBOB binding, [ 3 H]GABA uptake, cell viability, and morphology were assayed. (hindawi.com)
Although no progression to the adult stage, Caco-2 cell co-culture resulted in morphological differentiation of H. contortus L4 and larval viability for up to 28 days. (gla.ac.uk)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
Hit compounds from such screens are not necessarily effective on hypoxic and slowly proliferating cells in 3-D tumour tissue. (researchgate.net)
The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)
Conclusion: Neurons in culture can survive mTHPC-PDT under conditions sufficient to kill tumour cells and other nervous system cells. (open.ac.uk)
No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)

When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
Regulatory T cells are immune cells that in healthy people stop the immune system's response once an infection has cleared and prevent the immune system from attacking the body's own tissues and cells. (lupusresearch.org)
Cells Tissues Organs. (bvsalud.org)
In metabolic tissues such as the pancreas, adipose, muscle, and liver, laminin isoforms are expressed in discrete temporal and spatial patterns suggesting that certain isoforms may support the development and function of particular metabolic cell types. (bvsalud.org)
Here, we determined that LAMB1 is upregulated in gastric cancer tissues and contributes to cell growth and motility. (bvsalud.org)

Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. (stemcell.com)
Survey Report: Where is Pluripotent Stem Cell Research Now? (stemcell.com)
Human liver organoid (HLO) derived from human pluripotent stem cells offers. (springeropen.com)
Human pluripotent stem cells (hPSCs)-derived brain organ. (springeropen.com)
The evolution of pluripotent stem cell-derived retinal organoids (ROs) has brought remarkable opportunities for developmental studies while also presenting new therapeutic avenues for retinal diseases. (springeropen.com)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
This concept gained prominence following Shinya Yamanaka's discovery of induced pluripotent stem cells (iPSCs) in 2007. (scienceboard.net)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)

Later, with the assistance of two philanthropists, they established Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory where he furthered his work. (wikipedia.org)
He has also served as an investigator in a number of clinical projects undertaken by Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory. (wikipedia.org)
To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
In this post, we will explore two examples of how perfusion systems can help improve the relevance of your 3D cell culture, based on two publications from Alvetex users in the field of developmental biology and cancer. (reprocell.com)

Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
Organoids are cultured cellular structures that consist of the major cell types of a tissue including tissue stem cells and other differentiated cells, resemble the basic features and possess the functions of the tissue. (springeropen.com)
The first step is to develop and optimize a tissue culture and transformation system (protocols to introduce foreign DNA to new plants) for UF strawberry lines, so that new plants can be regenerated from cells containing introduced DNA. (ufl.edu)
The major strawberry cultivars grown in Florida such as 'Florida Radiance', Sweet Sensation® 'Florida127' and 'Florida Brilliance' were used for tissue culture optimization. (ufl.edu)
Basically, using one culture dish instead of 10 has allowed us to produce larger batches, faster, whilst using less tissue-culture real estate. (corning.com)
But recently, when a detection method for the crop became available (thus potentially causing canola imports to the EU to be rejected as unauthorised GMOs), Cibus did a U-turn and claimed that the crop was not after all produced using its much-hyped oligonucleotide-directed mutagenesis (ODM) method of gene editing, but was a product of random mutation in the Petri dish during the tissue culture phase of development! (gmwatch.org)
The new twist on this story is provided by the fact that Inf'OGM asked Cibus to clarify how it obtained its canola derived from GM transformation "event" 5715 - whether via ODM gene editing or random mutation in tissue culture. (gmwatch.org)
If the former, there is no way that Cibus could know whether canola 5715 obtained its herbicide-tolerant mutation via ODM gene editing or via spontaneous mutation in tissue culture. (gmwatch.org)
Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. (bvsalud.org)
Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. (bvsalud.org)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

The lead author recently provided ATLA with an update on the project: "In the submitted paper, we set up and characterised 20 different BBB in vitro models, based on the combination of epithelial or endothelial cell lines and primary or immortalised astrocytes/ glial cells or their derivatives. (frame.org.uk)
Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

Moreover, Laurent published a first-author video-protocol in JoVE , outlining how to monitor daily changes in clock proteins and hormone secretion in human primary cell cultures. (unige.ch)
Here, we report the use of a targeted liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method for concurrent quantification of multiple cytochrome P450 (P450), UDP-glucuronosyltransferase (UGT), and transporter proteins in cultured primary human hepatocytes. (aspetjournals.org)
Common culture formats used for in vitro enzyme induction studies comprise conventional and sandwich cultures, the former referring to plated hepatocytes maintained on rigid substratum (e.g., collagen), whereas in the latter cells are "sandwiched" by an additional layer of either collagen or a composite of extracellular matrix proteins, such as Matrigel ( LeCluyse, 2001 ). (aspetjournals.org)

We have now established a robust in vitro system for culturing and manipulating beta-cell precursors. (ca.gov)
The research group specialises in the development of in vitro models of different physiological barriers, such as the blood-brain barrier (BBB), pulmonary barrier and intestinal barrier, with a clear orientation toward the development of robust in vitro models according to the 3Rs principles. (frame.org.uk)

Background: The effect of photodynamic therapy (PDT) on neural cells is important when tumours are within or adjacent to the nervous system. (open.ac.uk)

In this thesis, human glia-to-neuron direct conversion and engineered viral vectors are explored using pre-clinical in vitro and ex vivo models. (lu.se)

Cell metabolism plays vital roles in organismal development, but it has been much less studied than transcriptional and epigenetic control of developmental programs. (springeropen.com)
It is actually programmed cell death essential for normal metabolism. (benthamscience.com)
During his studies he analyzed human muscle cells, publishing as first author a study in Molecular Metabolism showing that these cells possess their own circadian oscillators which control the normal or basal secretion of immune modulators called myokines, including IL-6, according to a day/night schedule. (unige.ch)
We conclude that targeted absolute LC-MS/MS quantification of drug-metabolizing enzymes and transporters can broaden the scope and significantly increase the impact of in vitro drug metabolism studies, such as induction, as an important supplement or future alternative to mRNA and activity data. (aspetjournals.org)

In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
Findings from this study will provide a foundation for clinical studies testing a potentially first-in-class therapy for lupus nephritis that uses specialized regulatory T cells to treat and even possibly cure this disease. (lupusresearch.org)
Considering that PTZ blocks the chloride channel coupled to the GABA A receptor complex, the present study aimed to analyze the possible modulation of GABAergic homeostasis within synaptic clefts in vitro . (hindawi.com)
These unique clones allow for to study into specific epicardial cell lineages and phenotypes. (arizona.edu)
Direct protein quantification was applied successfully to study in vitro induction in sandwich cultured primary hepatocytes in a 24-well format using the prototypical inducers rifampicin, omeprazole, and phenobarbital. (aspetjournals.org)
Therefore, selection of the appropriate culture format and conditions for a specific in vitro study is crucial with regard to the in vivo predictability of the data generated. (aspetjournals.org)
The purpose of this study was to investigate PDT using the photosensitiser, meta tetrahydroxyphenyl chlorin (mTHPC), on rat neurons and satellite glia, compared with human adenocarcinoma cell (MCF-7). (open.ac.uk)
On one hand, we are assessing the permeability of new drugs against prion diseases in a high-throughput screening study with the in vitro EPA BBB model. (frame.org.uk)
In this initial study a light-sensitive subexcitable Belousov-Zhabotinsky reaction in which a checkerboard image, composed of cells of varying light intensity projected onto the surface of a thin silica gel impregnated with a catalyst and indicator, is controlled using a learning classifier system. (mit.edu)
These 3D models are used to study how cells interact with each other within an organ, to gain insight into how disease processes disrupt these interactions, as well as to test the impacts of potential treatments. (corning.com)
In vitro study was categorized into groups G1 (iPS-MSC), G2 (iPS-MSC dOex-mIRs ), G3 (iPS-MSC + H 2 O 2 /100uM), and G4 (iPS-MSC dOex-mIRs + H 2 O 2 /100uM). (researchsquare.com)
Here, we show that the E12 or E13 SMG organ culture model can be used as an ideal approach to study the process of branching morphogenesis. (bvsalud.org)
Therefore, our study indicates that LAMB1 promotes cell growth and motility via the ERK/c-Jun axis and is a potential biomarker and therapeutic target of gastric cancer. (bvsalud.org)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)
This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)

Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)

Vγ9Vδ2 T cells are promising candidates for cellular tumor immunotherapy. (nature.com)
Techniques such as cellular reprogramming will allow the exploration of scalable allogeneic cell sources and the optimizable generation of cells for therapeutic development. (scienceboard.net)
Cellular reprogramming involves remodeling gene expression to generate a desired cell lineage or type. (scienceboard.net)
Advances in single-cell technologies and exponential growth of high-resolution biological data, harnessed by high-throughput bioinformatics and computational methodologies, have enabled the introduction of systematic means for controlling cellular identity. (scienceboard.net)
One mechanism of NK cell anti-tumor activity is antibody-dependent cellular cytotoxicity (ADCC) induced via Fc receptor (CD16) binding to antibodies. (maxcyte.com)
The ability to cultivate miniature cellular systems in vitro has become a powerful tool in disease modeling and drug discovery. (corning.com)
Organoids are cultured from stem cells within a 3D medium to provide a more physiologically relevant and realistic view of cellular interactions than can be seen with traditional 2D models. (corning.com)

Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Plasmids encoding these reporters were transfected into C2C12 myoblasts and fluorescence was analyzed as these cells underwent in vitro differentiation. (confex.com)

At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)

Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
12 Department of Neurosurgery and Center for Stem Cell & Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, Texas. (aacrjournals.org)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)

We also present a protocol for isolating epicardial clonal colonies from an epicardial cell line derived from the ImmortoMouse. (arizona.edu)

Challenge studies were conducted from September grown in cell culture, and no small animal model of human 2004 through October 2006. (cdc.gov)
Cells were grown for 2 weeks in DMEM : F12 containing 10% fetal bovine serum (FBS) until reaching confluence. (hindawi.com)
This led to the basis of cell culture studies, where cells grown in controlled artificial conditions simulate the conditions prevailing in natural ones, therefore, presumed to act as those in in vivo conditions. (benthamscience.com)
Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis. (reprocell.com)

The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
Adult-male SD rats (n=32) were equally divided into groups 1 (sham-operated control), 2 (DCM), 3 (DCM + iPS-MSCs/1.2 x 10 6 cells/administered by post-28 day's DCM induction) and 4 (DCM + iPS-MSC dOex-mIRs /1.2 x 10 6 cells) and euthanized by day 60 after DCM induction. (researchsquare.com)

This was subsequently put on clinical trial in 2011, reported to be the largest successful trial of stem cell therapy on humans, till then. (wikipedia.org)
Due to their HLA-independent mode of action, allogeneic Vγ9Vδ2 T cells can be considered for clinical application. (nature.com)
To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy, the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized, and clinical safety and efficacy also need to be proven. (nature.com)
Furthermore, a phase I clinical trial in 132 late-stage cancer patients with a total of 414 cell infusions unequivocally validated the clinical safety of allogeneic Vγ9Vδ2 T cells. (nature.com)
Our clinical studies underscore the safety and efficacy of allogeneic Vγ9Vδ2 T-cell immunotherapy, which will inspire further clinical investigations and eventually benefit cancer patients. (nature.com)
With his current LRA grant, Dr. Ooi will produce and test the effectiveness of "clinical-grade" regulatory T cells on lupus nephritis patient samples as well as in mice with lupus nephritis. (lupusresearch.org)
A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. (elifesciences.org)
Early studies on the transfer of unmodified autologous or allogeneic NK cells have established their clinical safety and demonstrated modest anti-tumor efficacy. (maxcyte.com)
Non-viral engineering of NK cells using MaxCyte ® mRNA electroporation provides significant benefits including high efficiency and low toxicity as well as clinical scalability enabling rapid development of novel adoptive cell therapy approaches. (maxcyte.com)
Building on his research at Cold Spring Harbor Laboratory, Tiriac is currently working on translating these protocols for clinical use. (corning.com)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
Secreted EVs from stem cells have become a hot topic with high research and clinical interest since they avoid concerns about the risks associated with directly injecting stem cells into the human body. (lu.se)

Primary cultures of cortical neurons and astrocytes were treated with EO (0-25%) for up to 90 min. (hindawi.com)
Primary cultures of neocortical neurons were obtained from 16-day-old NMRI mouse embryos (Charles River, Iffa Credo, Saint-Germain-sur-l'Arbresle, France) [ 5 , 6 ]. (hindawi.com)
The cell suspension for astrocyte culture was obtained in the same way as for neurons. (hindawi.com)
CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)

STAR Protocols. (amsterdamumc.org)

Three-dimensional (3D) in vitro culture can improve the performance and physiological relevance of immortalized and primary cells. (reprocell.com)

After 24h, basal medium was changed, apical medium was removed and cells were kept in air-liquid interface for 24h, before exposure. (nih.gov)
After that, basal medium was changed and cells were placed in a cell culture incubator for 24 hours. (nih.gov)
Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)

Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)

Coupled with well-established in vitro culture and expansion protocols for T lymphocytes, CAR enabled the antigen-directed cytotoxicity of T cells to be harnessed, expanding the scope of cell therapy. (scienceboard.net)

In scaffold-based culture, cells are supported in all dimensions either by an artificial structure or by a polymer network known as a hydrogel. (sigmaaldrich.com)
Both scaffolded and scaffold-free systems enable interaction in all directions with substrate, other cells, and extracellular factors. (sigmaaldrich.com)
MLO-Y4 osteocytes were cultured in Alvetex scaffold in either perfused or static conditions (using a perfusion plate or well inserts respectively) and the resulting conditioned medium was added to C2C12 myoblasts. (reprocell.com)

Establish overexpression of high-affinity CD16 on NK cells as a potential combination therapy for improving ADCC activity of anti-tumor monoclonal antibodies. (maxcyte.com)

They leverage transcriptomic and epigenetic data to systematically identify key regulatory switches, such as the optimal combination of transcription factors or growth factors, required to directly differentiate any source cell type to any target cell type , as well as maintain cell identity . (scienceboard.net)
Furthermore, we have used this protocol to differentiate PSCs from humans and chimpanzees. (lu.se)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)

This resistance was not due to differences in the cell cycle state of these inner cells since separate experiments showed that both exponential and plateau phase monolayer cells were about equally sensitive when the surviving fraction was plotted vs absorbed drug (D0, = 0.04 μ/106 cells). (researchgate.net)
This was likely due to the fact that we used transiently transfected cells, and so we started selection of stably transfected cells to isolate clones, which show permanent, and strong gene expression to carry out future experiments. (confex.com)
While naturally circulating tecovirimat-resistant monkeypox viruses have not been observed, previous cell culture experiments performed during drug development and independent studies performed prior to the current outbreak have demonstrated induction of resistance following tecovirimat exposure. (cdc.gov)

Previous studies from our laboratory have resulted in a short list of candidate regulators of beta-cell maturation. (ca.gov)
Our studies have identified several novel regulators of gene expression, including transcription factors and small non-coding RNAs, which we predict will have critical roles in beta cell maturation. (ca.gov)
Preliminary studies so far show that forced expression of select candidates in immature precursor cells can induce the expression of several genes critical for beta cell maturation. (ca.gov)
This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in blood and adds a functional dimension to conventional blood analysis. (elifesciences.org)

When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)

Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (sigmaaldrich.com)
U0126, an extracellular signal-regulated kinase (ERK) inhibitor, regulated the expression of LAMB1 in gastric cancer cells. (bvsalud.org)
During my thesis, we evaluated whether cell-derived strategies, in this case, extracellular vesicles (EVs) could be a potential new therapy to repair salivary gland injury and restore function following radiotherapy or in autoimmune diseases. (lu.se)

However, when stem cell-derived beta-cell precursors are transplanted into mice, they acquire properties of functional beta-cells, indicating that the precursors have the potential to transition into a mature beta-cell state. (ca.gov)

The Expert Committee on Biological Standardization reviews developments in the field of biological substances used in human medicine, which include vaccines, biological therapeutics, blood products and related in vitro diagnostic devices. (who.int)

I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)

Pretreatment of spheroids with misonidazole before ADR effectively reduced this resistant population of cells. (researchgate.net)
These clones provided direct evidence that the epicardium is a heterogeneous population of cells. (arizona.edu)
The cell population displayed an immunophenotype compatible to the one of stem cells, with remarkable positive expression of CD117. (bvsalud.org)
Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. (bvsalud.org)

We have also generated and tested requisite reagents for manipulating precursors in the culture dish. (ca.gov)
3 An up-to-date list of WHO International Standards and Reference Reagents is available at http://www.who.int/bloodproducts/catalogue/en/ (accessed 28 February 2013). (who.int)

Cultured hepatocytes offer the possibility to assess long-term effects, such as enzyme induction (i.e., changes of the expression levels of molecular entities of the hepatocytes). (aspetjournals.org)

Furthermore, the ability to isolate and expand cells of sufficient therapeutic quality and quantity in a patient-to-patient model can limit progress. (scienceboard.net)
This circumvents the need for any intermediary steps and reduces the time required to produce a cell type for therapeutic use. (scienceboard.net)
While increased CD16 expression was transient in nature, this may not negatively impact the therapeutic potential of engineered NK cells due to the short persistence of adoptively transferred NK cells. (maxcyte.com)
Dendritic cells-based therapeutic approach may be a potential strategy for SARS-CoV-2 infection. (medsci.org)

Spheroid culture is often favored for high-throughput compound screening in drug development and toxicology, where spheroids present more biologically-relevant models than 2D cultures. (sigmaaldrich.com)
The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
Next, we show that the proepicardium, which provides the precursor cells required for epicardial and coronary vessel development, migrates to the developing heart via direct contact of multicellular proepicardial villi to the developing myocardium. (arizona.edu)
Our research group continues its work on the development and improvement of in vitro models of the BBB, levelling up to a tri-culture model. (frame.org.uk)
Correct development of the musculoskeletal system relies on intricate signaling pathways and gene regulation that facilitate cell differentiation. (confex.com)
After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)

Dr. Ooi has pioneered the approach of developing specialized regulatory T cells that may be used as a future treatment for lupus nephritis patients. (lupusresearch.org)
show that this approach can detect characteristic changes that affect blood cells as a result of malaria, spherocytosis, bacterial and viral infections, and leukemia. (elifesciences.org)
As a result, developmental trends have shifted toward an allogeneic approach, seeking to replace autologous cell sources with consistent and scalable alternatives, such as iPSCs. (scienceboard.net)
The benefits of an allogeneic "off-the-shelf" cell therapy approach are clear: batch-producing cells derived from an unrelated donor or universal cell source that can be used to treat many patients. (scienceboard.net)

Traditional cell culture was developed on simple, nonporous two-dimensional (2D) surfaces, which facilitated the expansion of this vital technique across the life sciences. (sigmaaldrich.com)

As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)

Tiriac's lab then plates multiple domes, a 3D mix of cancer cells and Matrigel , into 6-well dishes containing this media. (corning.com)

A variety of techniques exist for sperm retrieval for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), including percutaneous epididymal sperm aspiration, microsurgical epididymal sperm aspiration, testicular sperm aspiration, and open testicular sperm extraction. (medscape.com)
in vitro fertilization. (who.int)

The beta-like cells generated with current protocols in vitro lack key features of normal beta-cells, most notably the ability to secrete insulin a regulated manner. (ca.gov)
Despite the rapid advancements in the field, current protocols still do not produce fully functional beta cells and the production of these cells takes at least six weeks. (ca.gov)

Anatomy15

Organisms13

Diseases15

Chemicals and Drugs30

Analytical, Diagnostic and Therapeutic Techniques and Equipment107

Phenomena and Processes48

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture6

Humanities3

Information Science20

Named Groups1

Health Care52

Geographicals4

Mesenchymal9

Assay6

Assays3

Embryonic stem9

Proliferation11

Pancreatic7

Therapies12

Hematopoietic Stem1

Fibroblast2

Methods9

Transplantation5

Phenotype4

Improve your 3D1

Viability3

Tumour5

Tissues6

Pluripotent10

Biology5

Tissue14

Glial3

Proteins3

Robust2

Neural Cells1

Vivo models1

Metabolism4

Study16

Replacement Therapy for Parkinson's Disease1

Cellular7

Underwent2

Progenitor cells2

Regenerative medicine3

Clonal1

Grown4

MSCs9

Clinical12

Neurons7

STAR Protocols1

Physiological1

Basal3

Differentiation of stem cells1

Cytotoxicity1

Scaffold3

Antibodies1

Differentiate3

Experiments3

Studies4

Laboratory1

Extracellular3

Stem cell-derived1

Therapeutics1

Predictive1

Organoid culture1

Population4

Reagents2

Enzyme1

Therapeutic4

Development8

Approach4

Expansion1

Results showed1

Matrigel1

Fertilization2

Current protocols2