CytotoxicityAssaysAutoimmune hemolysisVivo and in vitroMalariaToxicityHELLPCoagulationIntravascularHemagglutinationHypotonicHemolytic anemiaErythrocytesOccurHemoglobinAssay1994Biocompatibility testsAdditionallyAffectsExperimentsTestsAssessUnderstoodMeasurementsEffectsExtractsStudyProteinBlood cellsMethodsRiskTissueReactionsMaterialsDevelopmentalActivityHumanSamplesDataClinicalFreeStudiesFormation
Cytotoxicity4
- The PFTU NPs and PFTU@ DEX NPs showed no hemolysis and cytotoxicity. (nih.gov)
- Because the biocompatibility properties resulting from this new MIM cobalt alloy process are not well understood, we conducted tests to evaluate cytotoxicity (in vitro), hemolysis (in vitro), toxicity effects (in vivo), tissue irritation level (in vivo), and pyrogenicity count (in vitro) on such samples. (iospress.com)
- Blood hemolysis is a basic cytotoxicity (cell killing) test to see the interactions of a material with red blood cells (RBC). (purdue.edu)
- Influence of Crystallization Habit of Minerals on 'In Vitro' Cytotoxicity. (epa.gov)
Assays4
- The produced drug loaded nanoparticles were checked for drug release pattern and hemolysis assays. (ssrn.com)
- Venom toxicity and neutralization assays were as follows: An in vitro pharmacological evaluation was performed by direct hemolysis assay, indirect hemolytic assay, proteolytic activity, neutralization of procoagulant activity, and gelatin liquefaction method. (ijccm.org)
- We have a dedicated team that provides support for ex vivo pharmacodynamic (PD) and pharmacokinetic (PK) studies, and in vitro primary and cultured cell-based assays. (biocytogen.com)
- In vitro assays using patient muscle specimens revealed almost undetectable PFK enzyme activity, and patient blood samples exhibited erythrocyte PFK activity at about 50% of normal activity. (medscape.com)
Autoimmune hemolysis2
- Several reports suggest involvement of a drug-dependent autoimmune hemolysis mechanism ( 8 ), but systematic investigations have not been performed in most published cases ( 2 , 9 ). (cdc.gov)
- Materials and Methods: Patient samples sent for serological evaluation of autoimmune hemolysis were included in the study. (amrita.edu)
Vivo and in vitro2
- The inhibitory effects of CS-Ag hydrogel on disease in grape caused by P. expansum, A. niger and B. cinerea were investigated both in vivo and in vitro. (preprints.org)
- Mathematical modeling was used to estimate more generalizable key replication kinetic parameters from raw in vitro viral titers, revealing commonalities between viral infection progression in vivo and in vitro. (cdc.gov)
Malaria6
- Because the feeding process of the Plasmodium parasites damages red blood cells, malaria is sometimes called "parasitic hemolysis" in medical literature. (wikipedia.org)
- Episodes of delayed hemolysis 2-6 weeks after treatment of severe malaria with intravenous artesunate have been described. (cdc.gov)
- We performed a prospective observational study of patients with uncomplicated malaria to investigate whether posttreatment hemolysis also occurs after oral artemisinin-based combination therapy. (cdc.gov)
- Eight of 20 patients with uncomplicated malaria who were given oral artemisinin-based combination therapy met the definition of posttreatment hemolysis (low haptoglobin level and increased lactate dehydrogenase level on day 14). (cdc.gov)
- Given the key role of artemisinins in malaria treatment, WHO calls for prospective clinical studies and further research to improve the understanding of delayed hemolysis after artemisinin therapy ( 10 ). (cdc.gov)
- We hypothesize that delayed hemolysis occurs not only after intravenous treatment for severe malaria but also in a substantial number of patients given oral ACTs for uncomplicated malaria. (cdc.gov)
Toxicity2
- for developmental toxicity by combining in vitro toxicity data and calculated an even higher number of laboratory animals in silico kinetic modeling. (cdc.gov)
- In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
HELLP2
- Overexpression of both sEng and sFlt1 in pregnant rats develops nephrotic-range proteinuria, severe hypertension, biochemical evidence of HELLP ("H" for hemolysis, "EL" for elevated liver enzymes, and "LP" for low platelet count), and intrauterine growth restriction of the pups [ 19 , 20 ]. (hindawi.com)
- HELLP syndrome (hemolysis, elevated liver enzymes, low platelet count) may complicate severe preeclampsia. (medscape.com)
Coagulation1
- In vitro biocompatibility was studied for the first time and was evaluated by blood coagulation index (BCI) and haemolytic tests for using as wounds dressing. (ac.ir)
Intravascular3
- Intravascular hemolysis describes hemolysis that happens mainly inside the vasculature. (wikipedia.org)
- Intravascular hemolysis may occur when red blood cells are targeted by autoantibodies, leading to complement fixation, or by damage by parasites such as Babesia. (wikipedia.org)
- Intravascular haemolysis of vari- ous causes can result in acute tubular necrosis due to haemoglobinuria lead- ing to acute renal failure ( 12 ). (who.int)
Hemagglutination2
- No evidence of hemolysis, hemagglutination, or thrombocytopenia was observed in vitro or in cynomolgus macaques. (aacrjournals.org)
- A positive result occurs if there is clumping (hemagglutination) or rupture (hemolysis) of red blood cells exposed to the antiserum or typing reagent. (vin.com)
Hypotonic1
- It also inhibited the hypotonic solution-induced haemolysis by 73.19%, 49.69% and 29.15% at same concentration in membrane stabilizing assay. (banglajol.info)
Hemolytic anemia4
- Because in vivo hemolysis destroys red blood cells, in uncontrolled, chronic or severe cases it can lead to hemolytic anemia. (wikipedia.org)
- Extrinsic hemolysis is caused by the red blood cell's environment: Immune-mediated causes could include transient factors as in Mycoplasma pneumoniae infection (cold agglutinin disease) or permanent factors as in autoimmune diseases like autoimmune hemolytic anemia (itself more common in diseases such as systemic lupus erythematosus, rheumatoid arthritis, Hodgkin's lymphoma, and chronic lymphocytic leukemia). (wikipedia.org)
- Runners can develop hemolytic anemia due to "footstrike hemolysis", the destruction of red blood cells in feet at foot impact. (wikipedia.org)
- Echinocytes occur in many conditions, including malnutrition associated with mild hemolysis due to hypomagnesemia and hypophosphatemia, uremia, hemolytic anemia in long-distance runners, and pyruvate kinase deficiency . (medscape.com)
Erythrocytes2
- Hemolysis or haemolysis (/hiːˈmɒlɪsɪs/), also known by several other names, is the rupturing (lysis) of red blood cells (erythrocytes) and the release of their contents (cytoplasm) into surrounding fluid (e.g. blood plasma). (wikipedia.org)
- Hemolysis is usually self-limiting, but patients need to be actively followed up because transfusion of erythrocytes and rehospitalization might be necessary ( 2 , 5 ). (cdc.gov)
Occur3
- When performing venipuncture without adhering to the recommendations of good phlebotomy practice, a number of complications can occur, with in vitro hemolysis being particularly common ( 3 ). (biochemia-medica.com)
- In vitro hemolysis can occur from errors in specimen transport associated with the position of the tube (horizontal or vertical), mode and time of transport, as well as a temperature (extremely high or low), while the characteristics of centrifugation and separation are the factors affecting occurrence during the preparation of the blood sample for laboratory analysis ( 3 , 7 , 9 ). (biochemia-medica.com)
- Hemolysis may occur in vivo or in vitro. (wikipedia.org)
Hemoglobin1
- Hemolysis can lead to hemoglobinemia due to hemoglobin released into the blood plasma, which plays a significant role in the pathogenesis of sepsis and can lead to increased risk of infection due to its inhibitory effects on the innate immune system. (wikipedia.org)
Assay1
- Furthermore, in vitro scratch assay revealed that ZnFe 2 O 4 NPs improved cell migration and proliferation of cells, with noticeable shrinkage of the artificial wound model. (biomedcentral.com)
19941
- 1994. The SDA alternatives program phase III: Comparison of in vitro data with animal eye irritation data on solvents, surfactants, oxidizing agents, and prototype cleaning products. (cdc.gov)
Biocompatibility tests1
- Blood hemolysis and protein adsorption are the initial biocompatibility tests we are using to analyze cell interaction with our materials. (purdue.edu)
Additionally2
- Additionally, thrombotic microangiopathy (TMA) can result in hemolysis of red blood cells. (wikipedia.org)
- Additionally, hemolysis experiments and measurements of plasma-clotting time were carried out. (ncl.edu.tw)
Affects1
- In vitro hemolysis affects the quality of analyte and can lead to incorrect interpretation of obtained results ( 5 - 8 ). (biochemia-medica.com)
Experiments1
- Meta-analyses inclusive of IAV that display a diverse range of phenotypes in ferrets, interpreted with mathematical modeling of viral kinetic parameters, can provide critical information supporting a more rigorous and appropriate contextualization of in vitro experiments toward pandemic preparedness. (cdc.gov)
Tests2
- Nitrite both with and without nitrosatable precursors was found to be genotoxic in vitro in several tests, but, with the exception of a test in Drosophila melanogaster, the results obtained in vivo were negative. (inchem.org)
- In order to obtain fundamental data on the effects of inner shapes of connectors on blood, in vitro hemolysis tests were performed by using an original designed extracorporeal circuit. (elsevierpure.com)
Assess4
- This study aimed to assess confidence level of healthcare professionals in venipuncture and their knowledge on the possible causes of in vitro hemolysis. (biochemia-medica.com)
- Antibody coated red cells, either by in-vivo or in-vitro sensitization, were used to assess the outcome of three elution methods. (amrita.edu)
- This last topic is particularly relevant since pregnant women normally do not take part in clinical trials, making this human cell-based in vitro platform the only viable alternative to assess developmental cardiotoxicity. (scoop.it)
- In this study, an in vitro model is used to assess the antivenom capability of aqueous extract. (ijccm.org)
Understood2
- The pathophysiology of hemolysis after artemisinin therapy is not fully understood. (cdc.gov)
- By testing protein adsorption to a material, the resulting reaction the body can be better understood in an in vitro setting (outside of the body). (purdue.edu)
Measurements2
- As the relative contribution of in vitro experimentation to these rubrics has not been closely examined, we sought to evaluate to what extent viral titer measurements over the course of in vitro infections are predictive or correlates of nasal wash and tissue measurements for IAV infections in vivo. (cdc.gov)
- IMPORTANCE Both in vitro and in vivo models are employed for assessing the pandemic potential of novel and emerging influenza A viruses in laboratory settings, but systematic examinations of how well viral titer measurements obtained in vitro align with results from in vivo experimentation are not frequently performed. (cdc.gov)
Effects1
- Herein, we report in vitro anticancerous effects of doxorubicin (DOX) loaded on gold nanorods coated with the polyelectrolyte poly(sodium-4-styrenesulfonate) (PSS-GNRs) with and without NIR laser (808 nm, power density = 1.5 W/cm 2 for 2 min) irradiation. (beilstein-journals.org)
Extracts2
- The present study investigates the in vitro antivenom properties of Cyanthillium cinereum root extracts. (ijccm.org)
- By using in vitro techniques, the anti-venom activity of Cyanthillium cinereum plant root extracts against the venom of Naja naja (N. naja) and Daboia russelii (D. russelii) was examined. (ijccm.org)
Study2
- In vitro hemolysis study indicated that mPEG-PLA copolymers and ethaselen-loaded polymeric micelles had no hemolytic effect on the erythrocyte. (springer.com)
- Conclusions: The crossmatch results in this present study strongly demonstrates in-vitro incompatibility between canine and rabbit blood. (ed.ac.uk)
Protein1
- In in vitro anti-inflammatory test, the extract significantly inhibited protein denaturation by 85.17% at 500 ?g/ml, 71.72% at 250 ?g/ml and by 66.55% at 125 ?g/ml. (banglajol.info)
Blood cells1
- After being in contact with red blood cells for 1 h, the haemolysis ratio increased from to 0.46 to 1.4 when the concentration of goat GEL increased from 1 to 50 mg/ml suggesting the safety of the tested samples. (ac.ir)
Methods3
- Objective: To determine the in-vitro compatibility of rabbit and canine blood using both a tube and slide agglutination crossmatch technique and to compare the results obtained from these two methods. (ed.ac.uk)
- Both species-specific antisera and chemical reagents, directed against specific red blood cell antigens, are used in in-vitro blood-typing methods. (vin.com)
- In vitro test methods. (cvut.cz)
Risk1
- These analyses provide a necessary first step in enhanced interpretation and incorporation of in vitro-derived data in risk assessment activities and highlight the utility of employing mathematical modeling approaches to more closely examine features of virus replication not identifiable by experimental studies alone. (cdc.gov)
Tissue2
- Relating in vitro to kn viva exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
- 3D bioprinting techniques have brought considerable innovation in biomedicine, especially in the field of tissue engineering, allowing the production of 3D organ and tissue models for in vivo transplantation purposes or for in-depth and precise in vitro analyses. (preprints.org)
Reactions1
Materials2
- Confidence level at last venipuncture among both profiles of healthcare staff was very high, but they showed insufficient knowledge about possible factors affecting hemolysis due to materials used in venipuncture compared with factors due to venipuncture technique and handling of blood sample. (biochemia-medica.com)
- Material response: function and degradation of materials in vitro and in vivo. (cvut.cz)
Developmental1
- response curves for rat were found to be in concordance with the 2004), in vitro and in silico alternatives for developmental embryotoxic dose levels measured in reported in vivo rat studies. (cdc.gov)
Activity2
- SIRPα-Fc-CD40L activated a type I interferon response in macrophages and potentiated the activity of ADCP-competent targeted antibodies both in vitro and in vivo . (aacrjournals.org)
- The aqueous extract of the root of C. cinereum showed potent in vitro venom-neutralizing activity, and it can be used as a formidable therapeutic agent against N. naja and D. russelii envenomation. (ijccm.org)
Human2
Samples1
- Hemolysis in blood samples is usually a consequence of a number of factors, which can be classified as those occurring during venipuncture itself, during specimen transportation and while preparing the blood sample ( 3 , 9 ). (biochemia-medica.com)
Data1
Clinical1
- Clinical examina- was discharged home in good health ciency, who developed life-threatening tion also revealed gallop rhythm and a after having been counselled regarding haemolysis after application of henna short systolic murmur (haemic) on the the condition and given a list of drugs to his skin. (who.int)
Studies1
Formation1
- Like sFlt1, soluble endoglin (sEng) is an antiangiogenic factor capable of inhibiting capillary tube formation in vitro [ 17 ]. (hindawi.com)