CytotoxicityMiceHumansBiologicalVitroChemotherapyResistancePreclinicalCompoundsTumorEspecially for hypoxic cellsAntibodiesBlotCompoundMoleculesNSAIDsAnticancer drugsActivityDiseaseAnti-tumourConventionalBacteriaPharmaceuticalsLaboratoryPatientsAgarATCCChemicalInfectionTherapiesCancerDiscoveryCellsEffectsPharmacologyDevelopmentInterrogateIdentifyCellularHumanIncludeLargeDiagnosticTestMultipleStudiesTreatmentNatural
Cytotoxicity10
- Mytomycin C (MC), an antibiotic produced by S. lavendulae, exhibits cytotoxicity when the activated drug covalently binds complementary strands of DNA. (wikipedia.org)
- The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
- Prediction of Chemical Carcinogenicity in Rodents from in Vitro Genetic Toxicity Assays R AYMOND W. TENNANT, BARRY H. MARGOLIN, MICHAEL D. SHELBY, E RROL Z … As an in vivo toxicity endpoint, the acute lung inflammogenicity in a rat instillation model was compared with the in vitro toxicity endpoints comprising cytotoxicity, pro-inflammatory cytokine expression, or haemolytic potential. (holocausto.online)
- We conduct cytotoxicity estimation using a panel of cell proliferation and viability tests, which can be sorted into four types by different endpoints: Functional assays: assessment of ATP, ADP, ATP/ADP ratios. (holocausto.online)
- Kroll A(1), Pillukat MH, Hahn D, Schnekenburger J. The in vitro assays covered baseline toxicity in bacteria, oxidative stress, cytotoxicity in mammalian cells and yeast, estrogenicity, and anti-androgenicity. (holocausto.online)
- In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. (holocausto.online)
- Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
- It has been proven that combining electroporation with chemotherapy potentiates the cytotoxicity of drugs when the drugs' efficacy is limited by its uptake in the cell [ 9 - 13 ]. (hindawi.com)
- The aim of this study was to prepare and characterize new ruthenium NAMI-type compounds, to test their in vitro cytotoxicity and study the influence of electroporation on the cytotoxic activity of the synthesized compounds. (hindawi.com)
- The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
Mice2
- Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
- Re-expression of PLIN5 S155A in the liver of Plin5 liver-specific null mice reduced lipolysis compared with wild-type PLIN5 re-expression, but was not associated with other changes in hepatic lipid metabolism. (uci.edu)
Humans3
- The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE , infecting B-cells in humans. (lookformedical.com)
- In vitro toxicity assays are used to determine the potential of a new pharmaceutical, agrochemical, food additive, or other chemical product to be hazardous to humans.In vitro studies, which are performed on cultured bacterial or mammalian cells, can be used as an initial screen to avoid the unnecessary use of animals in determining which candidates should move forward for further safety testing. (holocausto.online)
- Therefore, the types of ious forms of radiation, carcinogen ifested as a higher incidence of the tumours that occur during childhood ic infectious agents, and chemicals same kinds of tumours that occur in in humans, including various embry and chemical mixtures. (who.int)
Biological2
- To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
- With the wide introduction of flow cytometry in the early 70-ies markers and subsequent cell types have evolved but it was only in the last decade that the introduction of single cell transcriptome sequencing, high dimensional cytometry and imaging cytometry started revolutionizing the way we interrogate biological samples. (frontiersin.org)
Vitro7
- The application of High Content Screening for in vitro toxicity testing is a relatively new approach in the preclinical research phase of drug development. (holocausto.online)
- In regulatory testing situation these in vitro toxicity assays must be reliable, because from these tests it is only possible to generalize the data to … First Published 1995. (holocausto.online)
- While toxicity test has been traditionally completed in the preclinical phase, in vitro toxicity studies in the early drug discovery stages could significantly reduce failures at a later stage and prevent economic loss. (holocausto.online)
- Considering the ethical issues and the cost of in-vivo animal tests, the pharmaceutical industry now relies more on in-vitro methods for toxicity testing in the drug development phase. (holocausto.online)
- Traditionally, the in vitro toxicity assays that have been used to identify molecules that carry drug-related DILI risk factors include two-dimensional (2D) cell systems and other standard preclinical model… The principal application of this assay is to rapidly screen test compounds to identify those that induce acute liver cell toxicity. (holocausto.online)
- H … In vitro assays covered baseline toxicity, oxidative stress responses, and estrogenic and antiandrogenic activity. (holocausto.online)
- The positive control induced a high degree of toxicity in all in vitro tests performed, indicating that the toxicity observed in the direct contact assay was due to in situ extraction of toxic components. (holocausto.online)
Chemotherapy2
- Lung cancer is a predominant type of cancer that causes high mortality, and the survival rate remains relatively low even after surgery, chemotherapy or radiotherapy [ 1 ]. (oncotarget.com)
- The aim is to try to identify drugs that may be effective in killing leukemia cells for those patients who will not be cured with conventional chemotherapy. (clincosm.com)
Resistance4
- This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
- These results demonstrate the importance of DCs in overcoming ICI resistance and achieving effective and durable antitumor responses and, in many cases, antitumor immunity. (izb-online.de)
- Emerging evidence suggests that they provide a cellular mechanism for induction and emergence of drug resistance and contribute to increased invasive and metastatic potential. (frontiersin.org)
- The development of platinum-drug resistance in cancer patients, the general toxicity and severe side effects of platinum drugs however required a different approach in the research of anticancer metal complexes [ 2 ]. (hindawi.com)
Preclinical2
- Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)
- Collectively, these data show that IM-T9P1 leads to long-lasting, adaptive antitumor immunity that can be further improved when combined with T cell-targeting immune checkpoint inhibitors and can have efficacy against large, established tumors in preclinical tumor models. (izb-online.de)
Compounds6
- Many natural compounds have led to the discovery of drugs used to treat human disease. (wikipedia.org)
- The inclusion of other assays and compounds will most likely expand the types of activities that can be reliably detected. (holocausto.online)
- Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)
- Our researchers are now extracting bacterial DNA from soil and marine habitats, cloning large fragments into, for example, bacterial artificial chromosomes, expressing in a host bacterium and screening the library for new compounds. (microbialtec.com)
- With an eye to filling those drying pipelines with an endless supply of potentially therapeutic compounds, companies heavily invested in ways to randomly generate tens of thousands of "drug-like" compounds from the chemical building blocks of known drugs (see sidebar, "Leaving Lipinski? (drugdiscoverynews.com)
- As the American Chemical Society 's Mark Lesney glibly wrote back in 2002: "A large enough library with the right high-throughput screening assay promised the ultimate brute-force method for obtaining bioactive compounds without biologists and organic chemists getting in the way. (drugdiscoverynews.com)
Tumor7
- We studied the chemosensitivity of these two MCSP tumor xenografts to anticancer drugs in vivo. (ox.ac.uk)
- The combined downregulation of PD-L1 expression in a single-molecule approach resulted in exceptional antitumor activity in different tumor models. (izb-online.de)
- Treatment with IM-T9P1 resulted in the sustained activation of a specific type of tumor infiltrating cell, dendritic cells 3 (DC3s), which can promote antitumor immunity by supporting T cells with survival signals and enabling them to perform their effector functions. (izb-online.de)
- Increasing amounts of evidence indicate that autophagy is induced by a number of stressors in tumor cells, such as starvation, growth factor deprivation, hypoxia, damage stimulation and therapeutic drugs, and is an important survival mechanism in response to cellular stress ( 9 ). (spandidos-publications.com)
- Discuss the current challenges and key considerations to be taken when designing assays to identify new biomarkers in pan tumor applications. (hub-xchange.com)
- Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. (medsci.org)
- In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods. (medsci.org)
Especially for hypoxic cells2
Antibodies2
- While the epitope of most B7-H3 antibodies has not been described, one of the clinically tested antitumor antibodies (8H9) was found to bind to the IgV domain 19 . (nature.com)
- When I was at GSK and also when I was with Shire , one of the things I really had antibodies to was this whole notion of industrializing drug discovery-at the end of the day, that's really what combinatorial chemistry and HTS is or was-because I really believe that drug discovery of new medicines is truly a creative process," he says. (drugdiscoverynews.com)
Blot2
- Corresponding cultures yielded a strain identified as M . haemophilum at the Netherlands National Institute for Public Health and the Environment (RIVM) by using the Inno-LiPA Mycobacteria v2 reverse line blot assay (Innogenetics, Ghent, Belgium). (cdc.gov)
- Using cDNA microarray screens and northern blot analysis, a previous study showed that Beclin 1 is upregulated in liver cancer tissues ( 11 ). (spandidos-publications.com)
Compound2
- Mammalian genotoxicity assay reveals if the compound causes abnormalities in chromosome distribution (aneugenity) or chromosome breaks (clastogenity) during cell division. (holocausto.online)
- In the 25 years from 1981 to 2006, they suggest, only one de-novo compound arising from combinatorial chemistry achieved regulatory approval, that being Bayer 's antitumor compound sorafenib. (drugdiscoverynews.com)
Molecules3
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- Discovery pipelines have been horribly inefficient, and if you look at the major pharmas, what they're doing typically is dismantling that industrial framework and moving much more toward either internal-focused discovery with very small discovery units-and that's certainly how GSK's done it-or partnering with academic units or highly creative companies like Ambrx to bring forward those creative molecules as rational drug design from different disciplines converges," he adds. (drugdiscoverynews.com)
- Isolation of multiple types of molecules (DNA, RNA, or protein) from a single cell simultaneously, stands at the beginning of each approach and having standardized and validated protocols for single cell solutions is surely the foundation of all the herein described approaches. (frontiersin.org)
NSAIDs3
- Non-steroidal anti-inflammatory drugs (NSAIDs) have a variety of potential indications that include management of pain and inflammation as well as chemoprevention and/or treatment of cancer. (nih.gov)
- Immunosuppressive agents encompassed a large number of drugs, mainly represented by anti-tumour necrosis factor (TNF)-α, anti-retroviral therapy (ARV), azathioprine (AZT), methotrexate (MTX), mycophenolate mofetil (MMF), non-steroidal anti-inflammatory drugs (NSAIDs), rituximab and steroids. (ersjournals.com)
- Patients can usually be treated with nonsteroidal anti-inflammatory drugs (NSAIDs) alone. (msdmanuals.com)
Anticancer drugs1
- The methanolic extracts of seaweeds Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata possess high total phenolic content and shows a good free radical scavenging activity and hence are proven to have better antioxidant activity and they might be good candidates for further investigations in order to develop potential anticancer drugs. (google.com)
Activity5
- In a continuation of our work probing the anticancer activity of small sulindac libraries, we have prepared and screened a small diversity library of α-methyl substituted sulindac amides in the profen class. (nih.gov)
- An MTT assay was used to determine cell metabolic activity and a TUNEL assay for detecting DNA fragmentation. (holocausto.online)
- The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. (cancerindex.org)
- MTT assay was employed to study the anticancer activity against cancer cell lines Hep-G2, MCF7 and normal VERO cell lines. (google.com)
- Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay. (google.com)
Disease6
- ICIs have transformed the treatment of cancer, producing responses in patients with various types of metastatic disease. (izb-online.de)
- On any given day, the media streams are filled with companies announcing their latest biologic achievements in medicine, and given the high profile of these therapeutics in disease areas such as oncology and autoimmunity, it is hardly surprising if some people have forgotten the role of medicinal chemistry in the drug discovery process. (drugdiscoverynews.com)
- The goal is to use this laboratory assay to best match a drug to a patient's disease. (clincosm.com)
- This assay best matches a drug to the patient's disease. (clincosm.com)
- There is still no drug that alters the state of the disease. (helsinki.fi)
- If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
Anti-tumour1
- These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
Conventional1
- A typical workflow for conventional bioprospecting and drug discovery. (microbialtec.com)
Bacteria1
- Table 1 Some FDA-approved drugs from bacteria. (microbialtec.com)
Pharmaceuticals1
- Prior to Flagship Biosciences, he served in senior scientific roles in oncology drug development for OSI Pharmaceuticals and Pfizer. (hub-xchange.com)
Laboratory2
- Normal human colon epithelial cell NCM460, human colorectal cancer cell lines (HCT 116 and SW620) and human embryonic renal cell lines (293 FT) were purchased from the American Type Culture Collection (ATCC, USA) and stored in our laboratory. (biomedcentral.com)
- This clinical trial uses a laboratory test called a high throughput sensitivity assay in planning treatment for patients with relapsed or refractory acute myeloid leukemia. (clincosm.com)
Patients4
- Otherwise, patients receiving at least one immunosuppressive drug (especially steroids) are more likely to have an indeterminate result [ 1 , 4 , 7 , 8 ]. (ersjournals.com)
- We made the hypothesis that the proportion of "bad" responders to mitogen among patients with negative QFT could be underestimated, especially during immunosuppressive drug intake. (ersjournals.com)
- Despite screening, mycobacterial infections have been diagnosed after prolonged treatment in various patients ( 8 ). (cdc.gov)
- Patients receive 1 of 160 possible interventions based on high throughput drug sensitivity assay. (clincosm.com)
Agar1
- The RIVM performed drug susceptibility testing by using a modified agar dilution method ( 5 ). (cdc.gov)
ATCC1
- ATCC, American type culture collection. (researchgate.net)
Chemical1
- Amongst all the marine invertebrates, sponges represent the most described group with potential application in medicinal chemistry, representing a valid starting point for new drug leads due to their chemical defense mechanisms. (microbialtec.com)
Infection2
- Notably, the tuberculin skin test result was negative at screening before she began the TNF-αA treatment, but was now positive (20 mm), suggesting mycobacterial infection. (cdc.gov)
- Side effects of concern are infectious diseases, which prompt the need for screening for latent mycobacterial infection before commencing treatment ( 7 ). (cdc.gov)
Therapies1
- This symposium presents a remarkable opportunity to convene with other researchers, clinicians, and industry leaders to discuss the extraordinary progress and immense potential of new drug therapies and technologies in paving the way for a brighter future in cancer treatment. (nyas.org)
Cancer2
- These drugs inhibit immune-suppressive signals and reinstate cancer immunosurveillance. (izb-online.de)
- These parts include a multitude of cell types that evolve during the course of carcinogenesis and cancer cell progression. (frontiersin.org)
Discovery5
- Further more, one of the most recent approaches in drug discovery from microorganisms is cocultivation. (microbialtec.com)
- If you are interested in microbial drug discovery services, please contact us for more details. (microbialtec.com)
- Part of the reason for medicinal chemistry's disappearing act may be due to attempts by the pharmaceutical industry itself to move medicinal chemists to the back burner by automating molecular discovery and synthesis through methods such as combinatorial chemistry and high-throughput screening (HTS). (drugdiscoverynews.com)
- We will also shortly discuss how leveraging single cell multi-omic approaches can be used to accelerate cellular biomarker discovery during clinical trials to predict response to therapy, follow responsive cell types, and define novel druggable target pathways. (frontiersin.org)
- Brad Nelson has twenty five years of experience in leading rapid growth life science organizations, delivering new technology and applications for drug discovery, development and clinical markets. (hub-xchange.com)
Cells3
- Extraction and direct contact assays were performed using human epidermal keratinocytes and mouse fibroblasts and mouse epithelial cells. (holocausto.online)
- The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
- In 2D culture, all cells can proliferate at a relatively constant rate in a planar space, and can be provided uniform access to nutrients, oxygen or drugs. (medsci.org)
Effects3
- Hepatotoxicity (toxic effects on the liver) and cardiotoxicity (toxic effects on the heart) are the most severe problems encountered in drug development and the major reason for drug withdrawals. (holocausto.online)
- Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)
- However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)
Pharmacology1
- Further applications are possible in various areas of pharmacology, developments catalyzed by the use of simple enzyme assays for screening before testing in intact animals. (microbialtec.com)
Development4
- Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
- Joseph Krueger serves as the scientific leader for Invicro's Advanced Pathology Services and is responsible for developing new biomarker applications to meet drug development needs. (hub-xchange.com)
- Prior to joining Invicro, Joseph was Chief Scientific Officer at Flagship Biosciences where he led the R&D and Scientific efforts in applying novel quantitative tissue image analysis approaches to immuno-oncology and rare diseases to support drug and companion diagnostic development. (hub-xchange.com)
- Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
Interrogate1
- Explore how novel tissue staining technologies can be applied to interrogate new drug targets and immune cell while preserving tissue architecture for spatial analysis. (hub-xchange.com)
Identify2
- Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
- Since the early days of cell biology scientists have been using optical instruments to identify cell types in homeostatic conditions and diseases. (frontiersin.org)
Cellular1
- In synergy with constantly adapting new single-cell analysis approaches and subsequent accumulating big data collections from these platforms, whole atlases of cell types and cellular and sub-cellular interaction networks are created. (frontiersin.org)
Human2
- The type species is HERPESVIRUS 1, HUMAN . (lookformedical.com)
- The human brain has to intervene much more than just random chance and happenstance, which is really what it's all about when it comes to combinatorial chemistry and random screening. (drugdiscoverynews.com)
Include1
- Factors that cause a low incidence of tumours in offspring after birth, compared contribute to fetal susceptibility to near the end of gestation, in offspring with the incidence and multiplicity of these agents include maternal, pla that were exposed trans placental y. the same types of tumours in their cental, and fetal metabolism, the im This pattern can be seen in the directly exposed mothers. (who.int)
Large1
- As urinary screens, they are noninvasve and apble on a large scale with current labo techniques For short-term esue, glucaric acid, thloetbers, and mug tests are usefl. (cdc.gov)
Diagnostic1
- Functional T-cell assays are exposed to bias at several levels (manufacturing, pre-analytical, analytical and immunological) [ 2 ] and, unfortunately, the lack of a gold standard precludes higher diagnostic accuracy. (ersjournals.com)
Test2
- Cytology/Membrane leakage assays: LDH, AK test. (holocausto.online)
- This assay will test multiple drugs simultaneously against a patient's own donated blood sample. (clincosm.com)
Multiple1
- Examine the importance of biomarker quantification when phenotyping multiple targets across cell types. (hub-xchange.com)
Studies1
- Tis a i p il y Im at bece m a e e rater tIu feti exposure isbeingm d Theex eUttowhichIntohtic c hemiaiscrooathep barriermqvary greadyt de-pendng on the type of epsues, tming in p and materal Wi better eqre measures, ep studies of halt probably Wm not ci ienobiotic fegotoc* agents in the environment. (cdc.gov)
Treatment2
- To obtain results from a high throughput drug sensitivity assay within 10 days, procure drug within 14 days and initiate treatment within 21 days. (clincosm.com)
- Whether treatment was administered in the time frame based on the high throughput drug screen. (clincosm.com)
Natural2
- Some aspects of recent research with natural products directed to produce anti-hypertensive drugs are discussed. (scielo.br)
- Drugs of natural origin have been classified as (i) original natural products, (ii) products derived or chemically synthesized from natural products or (iii) synthetic products based on natural product structures. (microbialtec.com)