Tumor stem cell assay. Medical search. Frequent questions

We found that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. (cdc.gov)
Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)
In addition, it is further related with occurrence and metastasis of malignant tumors. (medscimonit.com)
Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
Cancer stem cells, instrumental in metastasis, would seem to ignore mechanisms normally functioning in the removal of excess cells. (iospress.com)
These results indicate that CTCDOs recapitulate several features of colorectal CTCs and may be used to investigate the features of metastatic CRC cells, to identify new prognostic biomarkers and to devise new potential strategies for metastasis prevention. (biomedcentral.com)
Circulating tumor cells (CTCs) contain the physical elements responsible for tumor dissemination and metastasis. (biomedcentral.com)
It has been demonstrated that gastric cancer mesenchymal stem cells (GC-MSCs) can promote the progression, metastasis , and chemoresistance of GC through various mechanisms, but the effect of GC-MSCs on GC during chemotherapy is still unknown. (bvsalud.org)
Cancer stem-like cells (CSCs) are associated with cancer progression, metastasis, and recurrence, and may also represent a subset of circulating tumor cells (CTCs). (biorxiv.org)
Combined, these data indicate that CD117 expression potentially promotes tumor initiation and metastasis. (biorxiv.org)
The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
The CSC theory is attractive because it offers an explanation for tumor heterogeneity, for the inefficiency of metastasis formation and for tumor regrowth after an apparently complete remission. (medscape.com)

ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)
This study investigates whether UM progression is driven by a subpopulation of stem-like cells, termed "cancer stem cells" (CSCs). (whiterose.ac.uk)
Furthermore, there is an absence of a cellular hierarchy in cell lines and all cells in culture are able to drive tumor progression. (whiterose.ac.uk)
By targeting residual tumor cells with precise gamma ray doses, the procedure slows the progression of a returning brain tumor. (tgh.org)
EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
Our previous study showed that it plays an essential role in the progression of multiple tumors. (nature.com)
Tumor microenvironment (TME) remodeling is a critical process in primary tumor progression. (nature.com)
In cancer progression, FOXO3a inhibition stimulates cell transformation and angiogenesis ( 9 ). (spandidos-publications.com)
This editorial highlights the relevance of interfering in cancer cell progression through the pharmacological manipulation on the cell metabolism of cyclic nucleotides such as cAMP, and on the intracellular Ca2+ signaling, which may avail the reduction of toxic effects promoted by chemotherapy, radiotherapy and immunotherapy, thus decreasing the incidence of interruption in antitumoral treatment. (researchgate.net)
and melanoma tumor progression. (sigmaaldrich.com)
BACKGROUND: Breast cancer stem cells (BCSCs) have a crucial role in breast carcinogenesis, development, and progression. (harvard.edu)
During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
Further, in cell lines, CD117 activation by SCF promotes faster proliferation and invasiveness, while blocking CD117 activation with tyrosine kinase inhibitors (TKIs) decreased progression in a context-dependent manner. (biorxiv.org)
Efforts to link CD44 to CSCs and tumor progression should consider the expression of various CD44 isoforms. (lu.se)

Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. (nih.gov)
Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)
Glioma tumor tissues were collected from 3 patients who received surgery, and the glioma stem cells were then separated, cultured, and identified by flow cytometry. (medscimonit.com)
CTCs-derived organoids (CTCDOs) were characterized through proteome profiling, immunohistochemistry, immunofluorescence, flow cytometry, tumor-forming capacity and drug screening assays. (biomedcentral.com)
3) Flow cytometry (FCM) assay using CD44, CD24, and EpCAM. (harvard.edu)

SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)
This observation has led to the 'cancer stem cell' (CSCs) hypothesis, stating that a subpopulation of cancer cells survive therapy and lead to tumor relapse. (mdpi.com)
A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
Emerging evidence suggests that cancer stem cells (CSCs), a subpopulation of tumor cells, have the properties of self-renewal, heterogeneous progeny, drug-resistance, and carcinogenesis in vitro and in vivo ( 4 , 5 ). (spandidos-publications.com)
Therefore, a therapeutic strategy targeting CSCs may help cure malignant tumors, including SCLC ( 7 ). (spandidos-publications.com)
[ 5 ] Obviously, the study of CSCs in any given tumor type, by proteomics or other approaches, requires prior validation of their actual existence. (medscape.com)

Enhancer of zeste homolog 2 (EZH2) belongs to poly-sulfur protein family and can mediate cell proliferation and differentiation via the modulation of various genes expressions. (medscimonit.com)
This study investigated the effect of EZH2 expression on proliferation and tumorigenesis of brain glioma cells. (medscimonit.com)
Clonal formation assay analyzed the change of cell proliferation potency. (medscimonit.com)
Our data demonstrated that in brain glioma cells, the decrease of EZH2 level could suppress cell proliferation and tumorigenesis potency, and meanwhile inhibit the expressions of oncogenes including c-myc and Akt. (medscimonit.com)
This study aimed to investigate the relationship between miR-506 and proliferation and migration of breast cancer cells. (medscimonit.com)
Cell proliferation, cell counting, colony formation assay, and Transwell assay were applied to evaluate the proliferation and migration of breast cancer cells. (medscimonit.com)
Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
Inhibition of YAP or TGF-β breaks this loop and suppresses cancer stemness and proliferation In nude mice, RAD18 promoted subcutaneous transplanted tumor growth and M2-type TAM recruitment. (nature.com)
Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
Their larval and adult cells have narrow potencies, sometimes coupled to virtually unlimited … proliferation, and function in the growth, maintenance and regulation of body size. (iospress.com)
The embryos of larger arthropods and deuterostomes with well-provisioned eggs or viviparity, on the other hand, exhibit regulative development, while their larval "set-aside" or adult stem cells function in the growth, maintenance, and regulation of organ size coupled to constrained proliferation and cell turnover. (iospress.com)
Mammalian embryonic stem cells would seem adapted to rapid proliferation, functioning in part to enclose yolk or to acquire access to maternal resources. (iospress.com)
Mammalian adult stem cells resemble the blastomeres of planktonic and benthic organisms with small eggs and may have evolved in mature organisms as an adaptation to the growth and maintenance of tissues via proliferation and the regulation of organ size via cell loss (e.g., terminal differentiation). (iospress.com)
STEM CELLBANKER significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage. (onenucleus.com)
AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion, and proliferation. (onenucleus.com)
Furthermore, the conditioned medium of GC-MSCs pretreated with chemotherapeutic drugs was more effective in promoting the proliferation, migration, and stemness of GC cell lines than the conditioned medium of GC-MSCs without chemotherapeutic drugs treatment . (bvsalud.org)
A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
We demonstrate that CD117+ cells display increased proliferation and migration. (biorxiv.org)

Last, we show that established UM cell lines and UM STCs are plastic in nature and switch their phenotype in response to environmental stimuli. (whiterose.ac.uk)
miR-506 over-expression may thus be able to improve the malignant phenotype of breast cancer cells. (medscimonit.com)
Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
In addition, the cytokine byproduct TGF-β activates macrophages to have an M2-like tumor-associated macrophage (TAM) phenotype. (nature.com)
Using cell-based and biochemical assays, transcriptomic analyses, and patient-derived xenograft models, we find that knockdown of HDAC1 alone has profound effects on the glioma stem cell phenotype in a p53-dependent manner. (jci.org)
Tumors characterized as positive for the CD44+/CD24- phenotype by immunohistochemistry were associated to all isoforms except the CD44 standard (CD44S) isoform, which lacks all variant exons. (lu.se)

We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. (nih.gov)
miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. (nih.gov)
By combining those two observations, Wnt drives cancer but Wnt drives healthy stem cells as well, we came up with a novel marker, LGR5, which is now widely used as a stem cell marker and LGR5 helped us to find the colon stem cell but also stem cells of many other tissues. (ecancer.org)
In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
Among these, Raman spectroscopy has attracted increasing attention as a highly sensitive molecular imaging technique for studying complex biological systems, including cells, tissues, and various biological materials [ 4 ]. (medsci.org)
This technique allows non-destructive, label-free spectral imaging and analysis of cells, tissues, and nanoparticles [ 5 - 7 ]. (medsci.org)
Strategies for regenerative therapies in adult mammals, therefore, might be based on stimulating growth of adult stem cells or their surrogates in specific tissues rather than on introducing embryonic stem cells into adults. (iospress.com)
Fluorescence in-situ hybridization (FISH) assays on fresh (including Amniotic fluid, chorionic villi sampling, peripheral blood, product of conception, tumour tissue) and FFPE tissues. (kkh.com.sg)
4) RT-PCR profiler Arrays using stem cell (SC) panel of 84 genes for four group of cells (1) CD44 + /CD24 - /EpCAM - BCSCs, (2) CD44 + /CD24 - /EpCAM + BCSCs, (3) mammospheres, and (4) normal breast tissues. (harvard.edu)
A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)
Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)
Tumors, like the tissues from which they originate, contain a unique SC population that is capable of self-renewal and of sustaining tumor growth indefinitely. (medscape.com)

The permanent cell line (BXD-1425EPN), which was derived from a passage II xenograft tumor and has been passaged in vitro more than 70 times, expressed similar differentiation markers of the xenograft tumors, maintained identical chromosomal abnormalities, and formed tumors in the brains of SCID mice. (nih.gov)
Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
The HUCMSCs derived multiple donors were performed comprehensively analysis and potent assays including expressions of surface markers, viability, growth curve, karyotype analysis, tumorigenicity, differentiation potentials, and immune regulation capability. (researchsquare.com)
Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)
Proven capable of recreating aspects of organ structure and function in vivo, organoids are organ-like structures that can be prepared by 3D cell culture and differentiation of stem cells or organ progenitors. (onenucleus.com)
Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
The two populations were tested for differentiation similarities to iCAF and myCAF states through transcriptome-wide RNA-Sequencing (RNA-Seq) including comparison to an available iCAF-myCAF cell state atlas. (lu.se)
Osteogenic differentiation and bone formation assays in culture and in vivo were employed to gauge for origin in bone marrow-derived mesenchymal stem cells (bMSCs). (lu.se)

Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
Chemotherapeutic Drugs Endow Gastric Cancer Mesenchymal Stem Cells with Stronger Tumor-Promoting Ability. (bvsalud.org)

Using a sequence-aware multi-model PMD caller they developed, dubbed MMSeekR, the researchers uncovered a high level of heterogeneity at the methylation level and in the distribution of PMDs across tumor samples. (genomeweb.com)
Specifically, the concepts of tumour heterogeneity, oncogene addiction, non-oncogene addiction, tumour initiating cells, tumour microenvironment, non-coding sequences and DNA damage response will be reviewed. (bmj.com)
As a new type of probe, the heavy isotope of water has been widely used in the study of cell development, metabolism, tissue homeostasis, aging, and tumor heterogeneity. (medsci.org)
The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
The CSC is defined as a cancer cell with self-renewing capacity that can regenerate the original tumor in all its differentiated heterogeneity. (medscape.com)

Limited availability of in vitro and in vivo model systems has hampered efforts to understand tumor biology and test novel therapies for ependymoma, the third most common malignant brain tumor that occurs in children. (nih.gov)
2.2%) of CD133(+) stem cells that can form neurospheres and display multipotent capabilities in vitro. (nih.gov)
IFN-γ release assays (IGRAs) are in vitro blood tests that measure T-cell release of IFN-γ after stimulation with antigens unique to Mycobacterium tuberculosis . (atsjournals.org)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)
However, whether casticin inhibits in vitro carcinogenesis and cancer stem cell (CSC) characteristics, and whether casticin activates FoxO3a in small cell lung cancer (SCLC) cells remain unclear. (spandidos-publications.com)
However, whether casticin inhibits in vitro carcinogenesis and CSC characteristics in the SCLC H446 cell line, and activates FoxO3a remains unclear. (spandidos-publications.com)
These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals. (ucdenver.edu)
The material from each step isscreened using acellular and in vitro assays for evaluating general toxicity, mechanisms of toxicity, and macrophage function. (cdc.gov)

Specialized iPSCs are reprogrammed from adult skin or infant cord blood cells and can become any cell type in the body - a condition called pluripotency that mimics the function of human embryonic stem cells (hESCs). (neurosciencenews.com)
Researchers also compared the molecular and functional characteristics of iPSCs to human embryonic stem cells, which are used sparingly as a gold standard for benchmarking quality. (neurosciencenews.com)
For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
Profoundly different patterns of potency and division are exhibited by mammalian embryonic and adult stem cells. (iospress.com)
The cellular products of embryonic stem cells routinely come under global influences and give rise to the cells of germ layers and organ rudiments. (iospress.com)
and human embryonic stem cells(hESCs). (sigmaaldrich.com)
That's what it took for scientists to uncover how some harmless embryonic stem cells (ESCs) are programmed to become deadly cancer cells later on. (epigenie.com)

Little is known, however, about the metabolic pathways that drive the growth of individual glioblastoma subtypes - knowledge that is crucial for developing novel and effective targeted therapies that might improve treatment for these lethal tumors. (neurosciencenews.com)
Inhibiting ALDH1A3-mediated pathways slows the growth of mesenchymal glioma stem cells and might provide a promising therapeutic approach for glioblastomas with a mesenchymal signature. (neurosciencenews.com)
The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)
Likewise, strategies for the containment of cancer might be based on promoting normal pathways of cell loss, the basal mode for handling excess cells. (iospress.com)
We demonstrate marked suppression in tumor growth upon targeting of HDAC1 and identify compensatory pathways that provide insights into combination therapies for GBM. (jci.org)
Another important focus of the laboratory is development of live cell based assays for identifying molecules that specifically target key pathways in cancer such as cell cycle, proteasome-Ubiquitin pathway, angiogenesis, HIF and tumor stem cells. (rgcb.res.in)

A study led by researchers at The Ohio State University Comprehensive Cancer Center - Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC - James) has identified an abnormal metabolic pathway that drives cancer-cell growth in a particular glioblastoma subtype. (neurosciencenews.com)
The physician scientists sought to identify glioblastoma subtype-specific cancer stem cells. (neurosciencenews.com)
Glioblastoma accounts for about 15 percent of all brain tumors, is resistant to current therapies and has a survival as short as 15 months after diagnosis. (neurosciencenews.com)
The researchers then used microarray analysis and pre-clinical animal assays to identify two predominant glioblastoma subtypes, proneural and mesenchymal. (neurosciencenews.com)
Glioblastoma is the most common form of primary brain tumour. (bmj.com)

miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression. (nih.gov)
These findings suggest a tumor suppressor role for FOXO3a, which could constitute a potential target for cancer treatment. (spandidos-publications.com)
They demonstrated that type I MAGE plays a critical role in conferring resistance to chemotherapy and inhibition of apoptosis in myeloma cells through regulation of Bcl-2 family proteins and the tumor suppressor p53. (themmrf.org)
First, genomic bisulfite sequencing revealed that miRNA tumor suppressor genes (like miR-9, miR-124a1, miR-148a, miR-34b and miR-34c) are hypermethylated in cancer, yet unmethylated in stem cells. (epigenie.com)

Endothelial pipe formation assay Subconfluent HUVECs had been gathered and resuspended in restricting moderate (Moderate 200 with 0.2% LSGS) and treated using the indicated focus of exosomes. (exposed-skin-care.net)

However, the mechanism through which RAD18 influences triple-negative breast cancer (TNBC), especially the interaction between tumor cells and the tumor microenvironment, remains elusive. (nature.com)
First, they are investigating immunologic therapies with a focus on using cutting edge genomic and immunologic assays to map the interaction between myeloma and the immune system in the tumor microenvironment. (themmrf.org)
The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)

MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)
Teratomas, benign tumors containing the developing cells of different body parts, allowed researchers to see if the iPSCs could form the body's three basic germ cell lines - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
Being pluripotent means iPSCs can generate cells from each of the three basic germ cell lines that form the body - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
They analyzed the whole-genome bisulfite sequencing profiles of 45 esophageal tumors, representing both the squamous cell carcinoma and adenocarcinoma subtypes, and matched nonmalignant tissue. (genomeweb.com)
Prof Clevers talks to e cancertv at WIN 2014 about using single stem cells to grow tissue for cancer research and potentially cancer treatment. (ecancer.org)
If you take a stem cell from the stomach you'll grow a stomach organoid and the cells are quite dumb, they will make the tissue where they come from but they will never make anything else. (ecancer.org)
Now, in cancer it is the epithelium that is actually the malignant tissue and a tumeroid would be an organoid not grown from a normal stem cell but grown from a cell that comes out of a tumour. (ecancer.org)
Also when they are normal tissue people would have predicted that they would probably have to become cancer cells to grow for long times outside the body, that's not what we find. (ecancer.org)
There are a lot of ethics involved that are very complicated because we sequence patients and their tumours, people know how to solve that, but we also grow patients' tissue and their tumours and we don't want to recreate a problem that has existed with healer cells where the original patient never had consented that T-cells were used and they were spread around the world. (ecancer.org)
METHODS: Fresh tumor tissue samples were obtained from 31 breast cancer (BC) patients for (1) Mammosphere culture. (harvard.edu)
Epithelial tissue is one of the four major types of tissue found in animals, and is the only type of tissue that is able to form and maintain layers of cells that are just one cell thick. (elifesciences.org)
It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)

Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. (nih.gov)
Casticin, a natural polymethoxyflavone isolated from A. annua, V. trifolia, and V. agnus‑castus induces apoptosis in cancer cells by activating FoxO3a. (spandidos-publications.com)
Conversely, FOXO3a overexpression suppresses cancer cell growth, induces apoptosis, and reduces tumor size by regulating downstream effectors ( 10 ). (spandidos-publications.com)
Dr. Lim has more than 18 years of experience in cell sorting, cell cycle analysis, apoptosis and immunophenotyping. (sc.edu)

In immunocompetent persons, available data suggest that both assays have at least equal sensitivity to the TST, with improved specificity, for the diagnosis of LTBI ( 10 , 11 ). (atsjournals.org)
Many cancers re-emerge after treatment, despite the sensitivity of the bulk of tumor cells to treatments. (mdpi.com)
Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
Herein, we review findings supporting the applications of and research on heavy water in monitoring of bacterial metabolism, rapid detection of drug sensitivity, identification of tumor cells, precision medicine, and evaluation of skin barrier function and promote the use of heavy water as a suitable marker for the development of detection and treatment methodologies. (medsci.org)
Organoids are proving increasingly popular because they maintain genetic information, are CRISPR compatible, expand tractable cell line availability, make sharing libraries easier and are proving useful in drug sensitivity assays. (onenucleus.com)
Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)
Microfluidic systems provide numerous advantages for single cell analysis: economies of scale, parallelization and automation, and increased sensitivity and precision that comes from small volume reactions. (justia.com)

All five mice receiving the initial transplantation of the patient tumor developed intracerebral xenografts, which have since been serially subtransplanted in vivo in mouse brains for 4 generations and can be cryopreserved for long-term maintenance of tumorigenicity. (nih.gov)
Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. (elifesciences.org)

The findings, published recently in the Proceedings of the National Academy of Sciences , show that high levels of the enzyme drive tumor growth. (neurosciencenews.com)

Both EMT and CSC phenotypic changes are associated with tumor recurrence. (techscience.com)
Therefore, EMT-targeted therapy is necessary to effectively overcome tumor drug resistance and recurrence. (techscience.com)
The thrust area of research is to understand molecular mechanisms of chemo resistance and tumor recurrence in triple negative breast cancer. (rgcb.res.in)

Neurosurgeons at TGH perform laser interstitial thermal therapy (LITT) on patients with primary and metastatic brain tumors to reach otherwise inoperable lesions. (tgh.org)
TGH physicians were first in Hillsborough County to perform GammaTile procedures on patients experiencing recurring brain tumors such as glio- blastomas, gliomas and meningiomas. (tgh.org)
Whereas surgical resection, chemotherapy and radiation treatment have been the traditional approaches to combating these brain tumors, GammaTile offers a potentially life- prolonging option. (tgh.org)

Correspondingly, the inhibition of p-AKT reversed the tumor -promoting effect of the conditioned medium of GC-MSCs pretreated with chemotherapeutic drugs . (bvsalud.org)

PBMC fraction also contains progenitor populations, as demonstrated by methylcellulose based colony forming assays. (wikipedia.org)
Where indicated two labeled populations of HUVECs were generated after incubation of the cells in UF010 standard culture monolayer for 3 h with PKH26- or PKH67-labeled exosomes. (exposed-skin-care.net)
However, the vast majority of biological knowledge has emerged as a consequence of studying cell populations and not individual cells. (justia.com)
Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
Methods: Primary breast tumor-derived CAFs were transduced to express human telomerase reverse transcriptase (hTERT) and sorted into CD105 low and CD105 high populations using fluorescence-activated cell sorting (FACS). (lu.se)
[ 2 ] In addition, some tumor types do not appear to adhere to the CSC concept at all [ 3 , 4 ] or may have multiple independent tumor-initiating cell populations. (medscape.com)

SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)
Circulating tumor cells (CTCs) are responsible for the metastatic dissemination of colorectal cancer (CRC) to the liver, lungs and lymph nodes. (biomedcentral.com)
CTCs analysis through liquid biopsies provides not only a full repertoire of tumor biological material (including proteins, lipids, sugars and nucleic acids) but also a sample of living tumor cells endowed with metastatic ability [ 3 ]. (biomedcentral.com)
The CD44 cell adhesion molecule is aberrantly expressed in many breast tumors and has been implicated in the metastatic process as well as in the putative cancer stem cell (CSC) compartment. (lu.se)

Methods: Expression of postulated stem cell markers aldehyde dehydrogenase (ALDH), CD44, and CD133 was analyzed in UM cell lines and primary UM short-term cultures (STCs) established from tumor samples. (whiterose.ac.uk)
The cells were generated with a variety of genes, methods and cells of origin, such as adult skin or infant cord blood cells. (neurosciencenews.com)
How well the 58 iPSC lines met quality criteria depended on the origin of the reprogrammed cells (skin vs. blood, male vs. female) and specific reprogramming methods. (neurosciencenews.com)
The different methods included a variety of reprogramming genes, vectors (engineered viruses that deliver genetic material to cells), or the use of plasmids (small DNA molecules that can deliver reprogramming genes). (neurosciencenews.com)
Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)

CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers. (lu.se)
Unsupervised hierarchical cluster analysis of CD44 isoform expression data divided tumors into four main clusters, which showed significant correlations to molecular subtypes and differences in 10-year overall survival. (lu.se)

Results: We demonstrate that expression of ALDH, CD44, and CD133 does not select for a subpopulation of stem-like cells in either UM cell lines or UM STCs. (whiterose.ac.uk)
To determine how CD117 expression and activation by its ligand stem cell factor (SCF, kit ligand, steel factor) alter prostate cancer aggressiveness, we used LNCaP-C4-2 and PC3-mm human prostate cancer cells, which contain a CD117+ subpopulation. (biorxiv.org)

Subsequently, PBMCs are challenged with tumor antigens such as tumor stem cell antigen. (wikipedia.org)

Stably introduced into cancer cell lines, the system reports on elevated endogenous ADAR1 editing activity induced by interferon as well as knockdown of ADAR1 and ADAR2. (cancerindex.org)

Brain glioma is a type of common primary intracranial malignant tumor, the prognosis of which is frequently unfavorable. (medscimonit.com)
The effect on tumorigenesis potency of glioma stem cells was determined by mouse transplantation assay. (medscimonit.com)
Our study suggests that ALDH1A3 is a potentially functional biomarker for mesenchymal glioma stem cells, and that inhibiting that enzyme might offer a promising therapeutic approach for high-grade gliomas that have a mesenchymal signature," says principal investigator Ichiro Nakano, MD, PhD, associate professor of neurosurgery at the OSUCCC - James. (neurosciencenews.com)
Overall, our data suggest that a novel signaling mechanism underlies the transformation of proneural glioma stem cells to mesenchymal-like cells and their maintenance as stem-like cells," Nakano says. (neurosciencenews.com)
In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
Surprisingly, we find that HDAC1 is the essential class I deacetylase in glioma stem cells, and its loss is not compensated for by HDAC2. (jci.org)

Meanwhile, at the DMR level, the researchers found more than 10,000 hypo-DMRs between the two esophageal cancer types and also note that many of the methylation changes there were also present in normal cells. (genomeweb.com)

This program has a special focus on immunologic therapy in the setting of autologous stem cell transplantation for myeloma. (themmrf.org)
Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)

In a study published June 9 by the journal Stem Cell Reports , and funded by the National Heart, Lung, and Blood Institute, the multi-institutional research team reports on the comprehensive characterization of a large set of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)
We here demonstrated that casticin decreased sphere‑ and colony‑formation capabilities, and downregulated uPAR and CD133 in second‑generation spheres, which were considered as lung cancer stem‑like cells (LCSLCs), from SCLC H446 cells, in a concentration‑dependent manner. (spandidos-publications.com)
Small cell lung cancer (SCLC), a poorly differentiated and highly aggressive tumor, constitutes approximately 15% of all lung cancers ( 1 ). (spandidos-publications.com)
Normally, the PVRL4 gene is not active in breast epithelial cells, but its activity is detected in many breast, lung, and ovarian tumors. (elifesciences.org)

Taken together, our data demonstrated that GC-MSCs gained more tumor -promoting abilities during chemotherapy . (bvsalud.org)
Specific areas of interest include understanding the mechanisms, which allow cancer cells to develop resistance to chemotherapy at the cellular and molecular levels. (sc.edu)
[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)

This study shows that the mesenchymal subtype is the most aggressive subtype, that it has the poorest prognosis among affected patients, and that cancer stem cells isolated from the mesenchymal subtype have significantly higher levels of the enzyme ALDH1A3 compared with the proneural subtype. (neurosciencenews.com)
Gastric cancer (GC) is one of the most aggressive tumors and has a poor prognosis . (bvsalud.org)

In humans, lymphocytes make up the majority of the PBMC population, followed by monocytes, and only a small percentage of dendritic cells. (wikipedia.org)

Here we review the data for the use of QuantiFERON-TB Gold In-Tube and T-SPOT.TB, the two currently available IGRAs, in immunocompromised adults, including persons infected with HIV, patients with immune-mediated inflammatory disorders, candidates for treatment with tumor necrosis factor-α inhibitors, patients receiving hemodialysis, solid-organ transplant recipients, and patients with cancer. (atsjournals.org)
The regulatory relationship between the expression levels of inflammatory factors, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β, and the expression levels of miR-26 in the supernatant of each group of cells was determined by qPCR. (amegroups.org)

The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)

The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. (nih.gov)
MiR-506 mimics, inhibitor, and negative control (NC) were transfected into MDA-MB-231 breast cancer cells. (medscimonit.com)
We demonstrate that the transformation of breast cancer cells is dependent on PVRL4. (elifesciences.org)
used genetic techniques to silence PVRL4 in cells from breast tumors, they found that it reduced the formation of clusters by the cancer cells and also reduced their ability to grow in the absence of attachment. (elifesciences.org)
The cells' gene expression signatures were tested for association with clinical outcome of breast cancer patients using survival data from The Cancer Genome Atlas database. (lu.se)
Conclusions: We propose that iCAFs and myCAFs originate in interlobular and lobular fibroblasts, respectively, and more importantly, that the tumor-promoting properties of lobular fibroblasts render the TDLU an epicenter for breast cancer evolution. (lu.se)
We used q-RT-PCR and exon-exon spanning assays to analyze the expression of four alternatively spliced CD44 isoforms as well as the total expression of CD44 in 187 breast tumors and 13 cell lines. (lu.se)
Breast cancer cell lines showed a heterogeneous expression pattern of the CD44 isoforms, which shifted considerably when cells were grown as mammospheres. (lu.se)

TAMs are the major inflammatory cells in the TME, and are closely related to poor outcomes in several types of tumors [ 11 ]. (nature.com)
In ADSCs transfected with miR-26, the miR-26 expression in the cell supernatant was negatively regulated by the expression of inflammatory factors, TNF-α, IL-6, and IL-1β, in the cell supernatant. (amegroups.org)
To address this, we here established two autologous CAF lines representing inflammatory CAFs (iCAFs) and myofibroblast CAFs (myCAFs) and compared them with already established interlobular- and lobular fibroblasts with respect to their origin and impact on tumor formation. (lu.se)

The ChemoID functional anti-cancer assay targets live cancer stem cells along with the bulk of the tumor cells. (tgh.org)
Cancer stem cells are a population of slow-cycling cells within the tumour bulk, with self-renewal capacity that attracts interest as a therapeutic target. (lu.se)

Part of the Progenitor Cell Biology Consortium, the scientists are working to make sure this growing area of medical research is grounded in safe and sound science. (neurosciencenews.com)
Cell type-specific partially methylated domains and differentially methylated domains found in normal cells persist in malignant cells, researchers from Cedars-Sinai Medical Center in Los Angeles and elsewhere report in Genome Biology . (genomeweb.com)

In both 2D and 3D cultures, stemness marker gene expression is higher in CD117+ cells. (biorxiv.org)
Conversely, tumors with strong expression of the CSC marker ALDH1 had elevated expression of CD44S. (lu.se)

It also is vital that - prior to being instructed to become a specific cell type - iPSC lines continuously renew and expand in a blank slate form without the introduction of genetic errors. (neurosciencenews.com)
Considerable effort over the last decade has been directed towards developing integrated and scalable single cell genetic analysis on chip (7, 8). (justia.com)

This study explored the serum concentrations of miR-26 in patients with carotid atherosclerosis (CAS) and defined the roles and mechanisms of miR-26 derived from the exosomes of adipose-derived stem cells (ADSC-exos). (amegroups.org)

Additional confusion surrounds stem-cell surrogates, cache and reserve cells having some characteristics of stem cells and not others. (iospress.com)
Functional characteristics were assessed with respect to contractility in culture and interaction with tumor cells in mouse xenografts. (lu.se)

These findings reveal a novel mechanism for regulating AMPK/FoxO3a signaling in response to casticin, suggesting a new strategy for SCLC therapy by targeting cancer stem‑like cells. (spandidos-publications.com)

This indicates that therapies for high-grade gliomas should be personalized, that is, based on the tumor subtype instead of applying one treatment to all patients," he says. (neurosciencenews.com)
For this study, Nakano and his collaborators used cancer cells from 40 patients with high-grade gliomas, focusing on tumor cells with a stem-cell signature. (neurosciencenews.com)

Mitochondrial function or the ability to generate energy through OXPHOS (oxidative phosphorylation) is vital for cell homeostasis and its dysfunction has been linked to the pathogenesis of nearly all chronic diseases ( Pieczenik and Neustadt, 2007 ). (frontiersin.org)
External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)

Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries. (cancerindex.org)
Yet, suitable high-throughput editing assays are needed to efficiently screen chemical libraries for modifiers of ADAR1 activity. (cancerindex.org)
High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)
Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)

As the promise of using regenerative stem cell therapies draws closer, a consortium of biomedical scientists reports about 30 percent of induced pluripotent stem cells they analyzed from 10 research institutions were genetically unstable and not safe for clinical use. (neurosciencenews.com)
we've done it for mice with liver diseases where we treat the mice with liver stem cells or liver mini-organs, that's one thing, so regenerative medicine. (ecancer.org)
Consequently, use of organoid culture models is now widespread in areas including organogenesis models, drug testing, tumor / disease / infection models, toxicity screening as well as personalized & regenerative medicine. (onenucleus.com)

SCs are not only capable of maintaining themselves (self-renewal) but also give rise to a proliferative 'transit amplifying' compartment and ultimately to postmitotic differentiated cells with specialized functions. (medscape.com)

In conclusion, direct injection of primary ependymoma tumor cells played an important role in the generation of a clinically relevant mouse model IC-1425EPN and a novel cell line, BXD-1425EPN. (nih.gov)
Mantle cell lymphoma (MCL) is a lymphoproliferative disorder derived from a subset of naive pregerminal center cells localized in primary follicles or in the mantle region of secondary follicles. (medscape.com)

We then realised that LGR5 is a receptor for a very potent growth factor called R-spondin and we designed cultures where we can take, we think, any type of stem cell from an adult mouse or human, culture it in a small cocktail of growth factors, the most important being R-spondin, and then have these single stem cells grow out for as long as we want. (ecancer.org)
They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)

Rac1 enzyme is not included in the inhibitor screening assay kit. (bellbrooklabs.com)

Anatomy14

Organisms2

Diseases5

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes5

Metastasis12

Progression15

Flow cytometry5

CSCs6

Proliferation19

Phenotype6

Tissues15

Differentiation9

Mesenchymal stem3

Heterogeneity6

Vitro9

Embryonic7

Pathways6

Glioblastoma5

Suppressor4

Formation assay1

Microenvironment3

Tissue15

Apoptosis4

Sensitivity7

Vivo2

Drive tumor growth1

Recurrence3

Brain tumors3

Inhibition1

Populations6

Metastatic4

Methods7

Subtypes2

Subpopulation2

Antigens1

Knockdown1

Glioma6

Esophageal1

Autologous stem cell trans2

Lung5

Chemotherapy3

Prognosis2

Humans1

Necrosis2

Transplantation1

Breast8

Inflammatory3

Bulk2

Biology2

Marker2

Genetic2

Mechanisms1

Characteristics2

Findings1

Gliomas2

Homeostasis2

High-throughput4

Regenerative3

Self-renewal1

Primary2

Growth2

Inhibitor1