Transplantation hematopoietic stem cells adherent cells cultured. Medical search. Frequent questions

While the terms mesenchymal stem cell (MSC) and marrow stromal cell have been used interchangeably for many years, neither term is sufficiently descriptive: Mesenchyme is embryonic connective tissue that is derived from the mesoderm and that differentiates into hematopoietic and connective tissue, whereas MSCs do not differentiate into hematopoietic cells. (wikipedia.org)
There has been an increasing interest in recent years in the stromal cell system functioning in the support of hematopoiesis. (nih.gov)
The stromal cell system has been proposed to consist of marrow mesenchymal stem cells that are capable of self-renewal and differentiation into various connective tissue lineages. (nih.gov)
Data from preclinical transplantation studies suggested that stromal cell infusions not only prevent the occurrence of graft failure, but they have an immunomodulatory effect. (nih.gov)
We examined whether bone marrow-derived adherent stromal cell transplantation (BMSCT) could restitute irradiated intestinal stem cells niche and mitigate radiation-induced gastrointestinal syndrome. (elsevierpure.com)
CXCR4 is a G protein-linked seven transmembrane spanning receptor that has been identified as a receptor of stromal cell-derived factor-1 (SDF-1) for stem cells [ 22 - 24 ]. (hindawi.com)
The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
The stromal cell products responsible for these effects of estrogens have not been identified. (jci.org)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Stromal cell feeder layers (e.g. (stemcell.com)

Mesenchymal stem cells (MSCs) also known as mesenchymal stromal cells or medicinal signaling cells are multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give rise to marrow adipose tissue). (wikipedia.org)
Mesenchymal stem cells (MSCs), a term first coined by Arnold I. Caplan in 1991, are characterized morphologically by a small cell body with a few cell processes that are long and thin. (wikipedia.org)
However, MSCs are found in much higher concentration in the Wharton's jelly compared to cord blood, which is a rich source of hematopoietic stem cells. (wikipedia.org)
These cells have several advantages over bone-marrow-derived MSCs. (wikipedia.org)
MSCs have been seen to even differentiate into neuron-like cells, but doubt remains about whether the MSC-derived neurons are functional. (wikipedia.org)
Less quantity of transplanted mesenchymal stem cells (MSCs) influences the therapeutic effects on radiation-induced lung injury (RILI). (hindawi.com)
Previous studies have demonstrated that MSCs overexpressing Chemokine (C-X-C motif) receptor 4 (CXCR4) could increase the quantity of transplanted cells to local tissues. (hindawi.com)
It has been observed that mesenchymal stem cells (MSCs) could home and engraft within the injured tissues and differentiate into vascular cells and alveolar cells to regenerate the damage in the irradiated mouse lung [ 10 ]. (hindawi.com)
Open up in another window Shape 1 Phase comparison microscopic images displaying major cultures of human being bone tissue marrow produced mesenchymal stem cells (hBMMSCs) at passages P0 Mcl1-IN-9 (A) and P1 (B). Non-adherent cells are indicated by dark arrows in P0 (A). The hBM-MSCs at P1 exhibited short and epitheloid spindle shaped and morphology. (mingsheng88.org)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
Mesenchymal stem cells (MSCs) are the adult stem cells that have been studied in recent years and used to treat many diseases. (biomedcentral.com)
Thus, these properties of MSCs make these cells potentially ideal candidates for tissue technology. (stemcellsgroup.com)
Arnold Caplan (1991), coined the term "mesenchymal stem cell and stated that the cells as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies. (stemcellsgroup.com)
However, the first clinical trials of MSCs were completed in 1995 when a group of 15 patients were injected with cultured MSCs to test the safety of the treatment. (stemcellsgroup.com)
Mesenchymal stem/stromal cells (MSCs) can be isolated from neonatal tissues, most of which are discarded after birth, including placental tissues, fetal membranes, umbilical cord, and amniotic fluid. (stemcellsgroup.com)
Thus, neonatal tissues are easily available and they have biological advantages in comparison to adult sources that make them a useful source for stem cells including MSCs. (stemcellsgroup.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Suture mesenchymal stem cells (SuSCs), a heterogeneous stem cell population, belong to mesenchymal stem cells (MSCs) or skeletal stem cells (SSCs), with the ability to self-renew and undergo multi-lineage differentiation. (encyclopedia.pub)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
iii) absence of several haematopoietic and endothelial markers (ie, CD45, CD34, CD11b or CD14, CD79 or CD19 and HLA-DR in human MSCs). (bmj.com)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
On infusion, culture-expanded MSCs regulate inflammatory and immune responses and tissue repair. (bmj.com)
Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
Mesenchymal stem cells (MSCs) are a promising cell type for cell-based therapies. (bvsalud.org)
Amniotic epithelial cells (AECs) and mesenchymal stem cells (MSCs) were isolated from placenta (a source of neonatal stem cells) by explant culture method. (bvsalud.org)
Results indicated that placenta-derived AECs and MSCs were efficiently differentiated into skin cells, that is, keratinocytes and fibroblasts, respectively, as indicated by morphological changes, immunostaining, and polymerase chain reaction analysis. (bvsalud.org)
Mesenchymal stem cells (MSCs) are an attractive candidate for autologous cell therapy, but their ability to repair damaged myocardium is severely compromised with advanced age. (bvsalud.org)
In addition to their stem/progenitor properties, mesenchymal stromal cells (MSCs) possess broad immunoregulatory properties that are being investigated for potential clinical application in treating immune-based disorders. (pdffox.com)
Introduction Mesenchymal stromal cells (MSCs) are adult, fibroblast-like multipotent cells characterized by the ability to differentiate into tissues of mesodermal origin, such as adipocytes, chondroblasts, and osteoblasts (Friedenstein et al. (pdffox.com)
MSCs can be isolated based on their ability to adhere to plastic culture dishes, and they are capable of significant expansion by consecutive in vitro passaging (Pittenger et al. (pdffox.com)
In response to this challenge, the International Society for Cellular Therapy formulated minimal criteria for defining MSCs in order to create a broader consensus for more uniform characterization of these cells (Dominici et al. (pdffox.com)
2007). In addition to their stem/progenitor properties, MSCs have also been shown to possess broad immunoregulatory abilities and are capable of influencing both adaptive and innate immune responses. (pdffox.com)
This ability of MSCs to adopt a different phenotype in response to sensing an inflammatory environment is not captured in assays that are commonly used to characterize these cells, but it is crucial for understanding their therapeutic potential in immune-mediated disorders. (pdffox.com)
Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, cartilage, fat and muscle cells and are being investigated for their utility in cell-based transplantation therapy. (biomedcentral.com)
In addition, functional integration of QD labeled MSCs in an in vitro cardiomyocyte co-culture was assessed. (biomedcentral.com)
Finally, in a cardiomyocyte co-culture QD labeled MSCs were easy to locate and formed functional cell-to-cell couplings, assessed by dye diffusion. (biomedcentral.com)
In part, this is due to limited methods to track MSCs in vivo , precluding long-term functional studies of transplanted cells. (biomedcentral.com)
We assessed the ability to track QD labeled MSCs in an in vitro cardiomyocyte co-culture. (biomedcentral.com)
Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) hold great promise in the treatment of DM. (biomedcentral.com)
MSCs were isolated from human umbilical cord (UC-MSCs) and expanded in an in vitro culture system. (biomedcentral.com)
UC-MSCs were then cultured in the IPC induction and differentiation medium in the presence of laminin 411. (biomedcentral.com)
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
Mesenchymal stem cells (MSCs) have gained interest because of its potential application in regeneration medicine and cytotherapy. (biomedcentral.com)

The standard test to confirm multipotency is differentiation of the cells into osteoblasts, adipocytes and chondrocytes as well as myocytes. (wikipedia.org)
Recent data from in vitro models demonstrating the essential role of stromal support in hematopoiesis shaped the view that cell-cell interactions in the marrow microenvironment are critical for normal hematopoietic function and differentiation. (nih.gov)
In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
These cells evolve from the differentiation of neural progenitor cells (NPCs). (differencebetween.com)
They play a role in cell development and differentiation. (differencebetween.com)
Rebar N. Mohammed Hematopoietic stem cells (HSCs) are a rare population of cells that reside mainly in the bone marrow and are capable of generating and fulfilling the entire hematopoietic system upon differentiation. (karger.com)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
a) The cells should exhibits plastic adherence (b) The cell should possess specific set of cell surface markers, i.e. cluster of differentiation (CD) 73, D90, CD105 and lack expression of CD14, CD34, CD45 and human leucocyte antigen-DR (HLA-DR). (c) The cells should have the ability to differentiate in vitro into adipocyte, chondrocyte and osteoblast. (stemcellsgroup.com)
Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
low cell number in adult tissues, restricted expansion and differentiation capacity, and donor-related heterogeneity limit their use. (bvsalud.org)
mice could be achieved by reconstituting CD8+ T cells in deficient hosts suggesting that practical plasticity was still possible after thymic development differentiation and emigration. (siamtech.net)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
Here, we investigate the cytotoxic effects of in vitro QD labeling on MSC proliferation and differentiation and use as a cell label in a cardiomyocyte co-culture. (biomedcentral.com)
Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)

Rich source of Hematopoietic Stem Cells. (newcyte.com)

These stem cells are capable of differentiating into chondrocytes, cardiomyocytes, melanocytes, and hepatocyte‐like cells in vitro. (wikipedia.org)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
Granulosa cells were isolated and treated with SiAmh interference and hPMSC supernatant to observe the effects of AMH expression on granulosa cell apoptosis in vitro. (biomedcentral.com)
We employed in vitro cultures and two in vivo models to investigate whether androgens exert these effects directly on marrow lymphoid precursors or whether actions on marrow stromal elements are required. (jci.org)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
Quantum Dots (QDs) offer an alternative to organic dyes and fluorescent proteins to label and track cells in vitro and in vivo . (biomedcentral.com)
Fluorescent QDs label MSC effectively in an in vitro co-culture model. (biomedcentral.com)
Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)

Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
The key difference between hematopoietic stem cells and progenitor cells is that hematopoietic stem cells have the ability to differentiate into different types of cells while progenitor cells are more specific and they differentiate into target cells. (differencebetween.com)
Progenitor cells are descendants of stem cells that further differentiate into specialized cell types. (differencebetween.com)
Progenitor cells are the cells that originate from stem cells and further differentiate to create specialized cell types. (differencebetween.com)
Some cells differentiate into targeted cells, while other cells have the ability to differentiate into more than one type of cell. (differencebetween.com)
Hematopoietic stem cells differentiate into multipotent progenitor cells. (differencebetween.com)
These multipotent progenitor cells differentiate into either common myeloid progenitor (CMP) or common lymphoid progenitor cells (CLP). (differencebetween.com)
On the other hand, progenitor cells are more specific than hematopoietic stem cells and can differentiate into target cells. (differencebetween.com)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
Similarly, they are characterized by an extensive capacity for self-renewal, proliferation, potential to differentiate into multiple lineages and their immune-modulatory role on various cells. (stemcellsgroup.com)
Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)

These bone marrow stem cells do not contribute to the formation of blood cells, and so do not express the hematopoietic stem cell marker CD34. (wikipedia.org)

Insulin-like growth factor-I (IGF-I) and its receptor (IGF-IR) are essential for normal ocular development and are expressed in numerous ocular cell types including lens epithelial cells, retinal pigment epithelial cells, Müller cells and endothelial cells. (shengsci.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Furthermore, they can connect malignant cells to benign cells, including vascular endothelial cells. (frontiersin.org)
Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
After a constitutive activation of the Akt pathway was initiated, early follicles (50-150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) ( n = 32), gel ( n = 24) or within matrigel ( n = 27) in serum free media for 14 days. (biomedcentral.com)
Endothelial cells (ECs) may elaborate essential "angiocrine factors" involved in organ regeneration. (biomedcentral.com)
Current pathophysiology studies are focused on the origin of the antibody response, the nature of the antigenic complex and pathologic epitopes, the mechanisms of interindividual differences in platelet activation, and the functions of monocytes and endothelial cells. (immune-source.com)
At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)

Cells may be genetically modified either in vivo or ex vivo . (cellculturedish.com)
In ex vivo applications, cells are modified in culture, which also allows for cell expansion and analytical characterization prior to re-infusion of the treated cells. (cellculturedish.com)
There are several possible viral vectors systems available and the decision of which to use depends on many factors such as tissue tropism, desire for integrative or non-integrative modification, in vivo or ex vivo process, prior immune exposure, whether the target cell is replicating or non-replicating, safety, and others. (cellculturedish.com)
A dose escalation study was performed to determine in vivo efficacy of IgA3.0 ch14.18 in an intraperitoneal mouse model using 9464D-GD2 neuroblastoma cells as well as in a subcutaneous human xenograft model using IMR32 neuroblastoma cells. (bmj.com)
2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
Reactions against the dominating undescribed epitopes had been from the memory space T-cell subset and may even be recognized directly former mate vivo after Th2 cell enrichment. (enmd-2076.com)

Methodology/Principal Findings: Autologous bone marrow was cultured in mesenchymal basal medium and adherent cells were harvested for transplantation to C57Bl6 mice, 24 and 72 hours after lethal whole body irradiation (10.4 Gy) or abdominal irradiation (16-20 Gy) in a single fraction. (elsevierpure.com)
Development of viable autologous cell therapy for treatment of heart failure in the elderly requires the need to address MSC ageing. (bvsalud.org)
Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
6 Cosmetic-plastic surgeons have studied autologous fat grafting for structural augmentation via transplantation of lipoaspirants for many years. (journalofprolotherapy.com)

Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
The term encompasses multipotent cells derived from other non-marrow tissues, such as placenta, umbilical cord blood, adipose tissue, adult muscle, corneal stroma, or the dental pulp of deciduous (baby) teeth. (wikipedia.org)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
The original amount of cells in major monolayer cultures different from 1.4 0.4 106 to at least one 1.9 Mcl1-IN-9 0.6 106 cells (from 5 mL bone tissue marrow aspirate Mcl1-IN-9 cultured in three T175 cm2 flasks). (mingsheng88.org)
Open up in another window Shape 2 Representative Fluorescent triggered cell-sorting (FACS) evaluation showing the Compact disc marker expression design in human being bone tissue marrow mesenchymal stem cells (hBMMSCs). (mingsheng88.org)
Progenitor cells are capable of differentiating into cells that belong to the same tissue or organ of each progenitor cell. (differencebetween.com)
Both cells have common applications in various cell-based therapies such as tissue regeneration and transplantation. (differencebetween.com)
We used the CliniMACS Plus system located in our Cell and Tissue. (karger.com)
The easy accessibility of hematopoietic cells, which are not tightly embedded in tissue, has supported. (karger.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
After hPMSC transplantation, the AMH and FSHR expression in ovarian tissue was significantly higher than in the POF group as determined by immunochemistry and western blot analysis. (biomedcentral.com)
The first source of Mesenchymal stem cells was in the bone marrow and considered to be the gold standard for clinical research, although various other sources have being discovered which include: Adipose tissue, Dental pulp, Mobilised Peripheral blood, Amniotic fluid, Joint synovium, synovial fluid, Endosteum, Periosteum, Menstrual blood and birth-derived tissues. (stemcellsgroup.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
Worldwide, 50,000 hematopoietic stem cell transplant (HSCT) procedures are undertaken annually to replace a person's blood-forming cells - hematopoietic stem cells (HSCs) - to treat blood disorders and cancers, yet two-thirds of patients who need HSCT lack matched donor tissue. (utoronto.ca)
Pathogenic transformation of Tregs in addition has been defined in various other experimental versions (25 26 Furthermore both maintenance of suppressive actions in peripheral tissue and the legislation of endogenous creation of IL-6 by nTregs had been been shown to be dependent on the current presence of Compact disc8+ T cells (21). (siamtech.net)
indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
However, its role in aging-related adipose tissue CD4+ T cell activation remains to be investigated. (exposed-skin-care.net)
CD8+ T Cell Activation Compared to CD4+ T cells, CD8+ T cells show a greater increase in adipose tissue in obesity and in aging (31, 43, 106). (exposed-skin-care.net)
Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)
The mechanism for CD8+ T cell activation in adipose tissue is not fully understood. (exposed-skin-care.net)
showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
Indeed, CD8+ T cells from mouse adipose tissue respond to cytokines and become activated and proliferate under stimulation of IL-12 and IL-18, which are mainly produced by APCs and are elevated in obese adipose tissue (44). (exposed-skin-care.net)
One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
Proteomics is a powerful approach to study the whole set of proteins expressed in an organism, organ, tissue or cell resulting in valuable information on physiological or pathological state of a biological system. (oaji.net)
Tissue from the upper or lower respiratory tract, sputum samples, and samples obtained by nasopharyngeal washing, bronchoalveolar lavage, and biopsy may be submitted for viral culture. (medscape.com)
One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)

Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)

A cell can be classified as an MSC if it shows plastic adherent properties under normal culture conditions and has a fibroblast-like morphology. (wikipedia.org)

Progenitor cells are an intermediary step in the development of mature cells in tissues, organs, blood, and the central nervous system. (differencebetween.com)
Therefore, progenitor cells are necessary for repairs and to maintain tissues after an injury. (differencebetween.com)
They can create new tissues and even whole organs from a few stem cells. (differencebetween.com)
Cells Tissues Organs (2021) 209 (4-6): 209-214. (karger.com)
Cells Tissues Organs (2019) 207 (1): 15-20. (karger.com)
Cells Tissues Organs (2008) 188 (1-2): 139-149. (karger.com)
Cells Tissues Organs (2008) 187 (3): 186-198. (karger.com)
Bone marrow-derived stem cells Hematopoietic stem cells Adipogenesis Fibrin gel culture, 3-dimensional cultures Original Paper Cells Tissues Organs 2008;187:186 198 DOI: 10.1159/000111804 Accepted after revision: August 29, 2007. (karger.com)
Cells Tissues Organs (2005) 180 (4): 195-203. (karger.com)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
These elaborate signals promoted by ECs have been termed "angiocrine factors" and have been shown to be crucial for the maintenance of organ-specific tissues and tumor cells [ 12 ]. (biomedcentral.com)
Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)

This study was performed to determine the effects of human placenta mesenchymal stem cell (hPMSC) transplantation on granulosa cell apoptosis and anti-Müllerian hormone (AMH) and follicle-stimulating hormone receptor (FSHR) expression in autoimmune drug-induced premature ovarian failure (POF) mice. (biomedcentral.com)
hPMSC transplantation was conducted by intravenous injection into mice following pZP3 treatment. (biomedcentral.com)
Morphological staining showed that the basal follicles and sinus follicles after hPMSC transplantation were higher in POF mice than in those without treatment, and the follicle number was significantly decreased with atresia. (biomedcentral.com)
hPMSC transplantation can significantly improve the serum levels of high gonadotropin and low estrogen of POF mice, promote follicular development, inhibit excessive follicular atresia and granulosa cell apoptosis, and improve the ovarian reserve capacity. (biomedcentral.com)
Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
Androgens only affected B cell development in chimeric mice with androgen-sensitive stromal cells. (jci.org)
Using knockin mice expressing functionally stunted β 3 integrin, we show that bone marrow transplantation rescues impaired angiogenesis in these mice by normalizing BMDC recruitment. (rupress.org)
In the lack of or disturbance with MHC I-CD8 connections the regulatory actions of nTregs had been altered not merely leading to the increased loss of suppression however in their transformation to pathogenic IL-13-making Compact disc4+ T effector cells improving lung allergic replies in receiver mice (5). (siamtech.net)
Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
Studies carried out in mice possess demonstrated the introduction of allergic airway hyperresponsiveness mediated by T cells in the lack of IgE (10, 11). (enmd-2076.com)
Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)

In the present study, we conducted overexpressing CXCR4 human umbilical cord mesenchymal stem cell (HUMSC) therapy for RILI. (hindawi.com)

There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Natural killer (NK) cells can kill cancer cells in the absence of prior stimulation and hold considerable potential for cell-based therapies targeting human malignancies ( 1 - 4 ). (aacrjournals.org)
Recent studies indicate that stem cell-based regenerative therapies can restore severe damaged skin both structurally and functionally. (bvsalud.org)
The methods proposed are cutting-edge and no other team in the world has this combination of technologies, approaches and expertise to make advances in hematopoietic cell therapies. (utoronto.ca)
However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
However, for cell therapies MSC have to be expanded and/or manipulated to obtain a sufficient amount of cells that can be subsequently used for treatment. (biomedcentral.com)

Conclusion/Significance: Mitigation of lethal intestinal injury, following high doses of irradiation, can be achieved by intravenous transplantation of marrow-derived stromal cells, including mesenchymal, endothelial and macrophage cell population. (elsevierpure.com)

studies indicated that RANTES is Purpureaside C an adipokine that can be produced by adipocytes and takes on an important part in T cell migration, suggesting a potential part of the RANTES/CCR5 axis in adipose T cell build up in obesity (24). (exposed-skin-care.net)
Taken together, one important mechanism for obese adipose CD4+ T cell activation may be mediated through MHCII expressed on ATMs and adipocytes. (exposed-skin-care.net)

Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)

In the presence of a genetic lesion, downregulation of SETD2 contributed to both initiation and progression during leukemia development by promoting the self-renewal potential of leukemia stem cells. (stemcellsciencenews.com)

Here, a combination of different cell surface markers is used to separate the rare HSCs. (differencebetween.com)
Scientists demonstrated that murine tumor propagating cells with the markers Sca1 and CD24 are enriched for metastatic potential in orthotopic transplantation assays. (stemcellsciencenews.com)
The group plans to identify better markers to track and purify key cell intermediates as well as uncover key genes/pathways that lead to HSC development. (utoronto.ca)

ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

Researchers report that loss of Wnt-1-induced signaling protein-2 (WISP2) in MCF7 breast cancer cells can also promote the emergence of a cancer stem-like cell phenotype characterized by high expression of CD44, increased aldehyde dehydrogenase activity and mammosphere formation. (stemcellsciencenews.com)
Sickle cell disease is inherited in an autosomal recessive manner when patients have 2 abnormal beta-globin genes and at least 1 of the beta-globin mutations is Hb S. Hb S can also be inherited with other mutations such as Hb C (Glu6Lys) or beta thalassemia, which results in a sickle phenotype. (medicalhomeportal.org)
Herein, the growth and proliferation, gene expression, multipotency and phenotype of cultured hBMSCs were compared with those incubated with CTX. (ijbs.com)

IgA3.0 ch14.18 was evaluated in binding assays and in ADCC and antibody-dependent cellular phagocytosis (ADCP) assays with human, neuroblastoma patient and non-human primate effector cells. (bmj.com)
Results ADCC and ADCP assays showed that particularly neutrophils and macrophages from healthy donors, non-human primates and patients with neuroblastoma are able to kill neuroblastoma tumor cells efficiently with IgA3.0 ch14.18. (bmj.com)
With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)

The cells do not have the capacity to reconstitute an entire organ. (wikipedia.org)
With the increase in organ transplantation and improved survival of transplant recipients, the incidence of disease will likely increase in the coming years. (cdc.gov)
In this study, we sought to describe characteristics, risk factors, and outcomes of Rhodococcus infection among solid organ transplant (SOT) and hematopoietic stem cell transplant (HSCT) recipients in the United States. (cdc.gov)
[ Kato: 2018 ] Chronically, sickle cell disease affects all organs in the body and can lead to end-organ damage in the brain, heart, lungs, and kidneys (as well as other areas). (medicalhomeportal.org)

Preclinical and early clinical safety studies are paving the way for further applications of mesenchymal stem cells in the field of transplantation with respect to hematopoietic support, immunoregulation, and graft facilitation. (nih.gov)
Case-patients were those with clinical or radiographic features of infection and positive culture results for Rhodococcus spp. (cdc.gov)
These cell types are used in cell culture studies to analyze cellular responses in different clinical and physiological settings. (differencebetween.com)
This promising field may open new avenues to modulate inflammatory responses and to preserve blood cell homeostasis in the clinical setting. (haematologica.org)
In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
We therefore adapted the K562-mb15-41BBL stimulation method to large-scale clinical-grade conditions, generating large numbers of highly cytotoxic NK cells. (aacrjournals.org)
The results that we report here provide rationale and practical platform for clinical testing of expanded and activated NK cells for cell therapy of cancer. (aacrjournals.org)

As many as 1 in 100 cells collected during amniocentesis are pluripotent mesenchymal stem cells. (wikipedia.org)
At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)

Further study will be required in the future to better understand succinate biology in blood cells. (haematologica.org)
This team, which brings together expertise in stem cell biology, cell biology, developmental signalling pathways, and genetic screens, aims at improving methods to achieve generation of HSC. (utoronto.ca)
His work covers various topics, including B cell responses to viral infection and vaccination, human primary immunodeficiency, and biology of lymphocyte development and function and to elucidate etiology of immunological disorders. (stanford.edu)

Hematopoietic stem cells (HSCs) are immature cells found in the peripheral blood and bone marrow. (differencebetween.com)
HSCs develop into different types of blood cells in lines called myeloid and lymphoid , which are involved in dendritic cells formation. (differencebetween.com)
Thus, alternative sources of patient-specific hematopoietic stem cells (HSCs) are required. (utoronto.ca)
The generation of HSCs has been a long-standing goal of stem cell biologists working in the field of developmental hematopoiesis. (utoronto.ca)

Blood glucose, insulin levels, and survival of rats were monitored periodically following intravenous injection of the tested cells. (biomedcentral.com)

Proliferating endothelial cell-specific expression of IGF-I receptor ribozyme inhibits retinal neovascularization. (shengsci.com)
Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)
Recent evidence obtained in mouse models shows its essential role regulating blood cell function through various mechanisms that include pseudohypoxia responses by hypoxia-inducible factor-1α activation, post-translational modifications like succinylation, and communication mediated by succinate receptor 1. (haematologica.org)
GPR91 is a G protein-coupled cell surface receptor for extracellular succinate (Sucnr1). (haematologica.org)

Conclusions: hBMMSC cell pellets are better protected from temperature alterations compared to cell suspensions. (mingsheng88.org)

Transplantation of enriched myeloid and non-myeloid fractions failed to improve survival. (elsevierpure.com)

Timm Schroeder Hematopoietic stem cell research has made tremendous progress over the last decades, and blood has become one of the best understood mammalian stem cell systems. (karger.com)
An invasive Escherichia coli expressing the inv gene from Yersinia pseudotuberculosis was used as a vector for protein delivery to mammalian epithelial cells. (shengsci.com)
Newcyte is performance tested to generate a linear fluorescent signal across a 1000-fold range of adherent mammalian cells. (newcyte.com)

Nevertheless, with following passages where standard monolayer cultures had been acquired, the cell amounts could be extended to 2.1 0.4 106 cells per T175 cm2 flask. (mingsheng88.org)
Scientists showed that MMTV-Her2/Neu mammary tumor cells cultured as non-adherent spheres or as adherent monolayer cells select for stabilizing mutations in p53 that "immortalize" the cultures, and that after serial passages, sphere conditions maintain tumor-initiating cells whereas monolayer cells gradually lose these tumorigenic cells. (stemcellsciencenews.com)

In addition to limiting HSC numbers, HSCT is inefficient, as many HSC do not get to the relevant areas in the body and/or die following transplantation. (utoronto.ca)

7 There has been some variation and question regarding the correct terminology for this population of stromal adipose cells. (journalofprolotherapy.com)
Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)

Results Morphology and growth features of hBMMSCs In major cultures by day time 5C7 the hBMMSCs honored the culture surface area as multiple colony developing units (CFU) as well as the cell amounts continued to increase by day time 7C9 achieving up to 60C70% confluence. (mingsheng88.org)
MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)

In the central nervous system of humans, there are three types of fully differentiated cells known as neurons, astrocytes, and oligodendrocytes. (differencebetween.com)

Transplantation of hBMMSCs as pellets rather than as cell suspensions to the cartilage defect site would therefore support their viability and may aid enhanced cartilage regeneration. (mingsheng88.org)

This was associated with increased infiltration of macrophages, lymphocytes, and mast cells. (oaji.net)

Study regulators of myelopoiesis in long-term cultures. (stemcell.com)

Maintenance of the hematopoietic stem cell population has been used to increase the efficiency of hematopoietic stem cell gene transfer. (nih.gov)
Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
The majority of gene therapy applications in development utilize viral vectors to carry the therapeutic gene into the target cells. (cellculturedish.com)
Expression cassettes are expressed within the cell resulting in viral proteins and a genomic ssDNA containing the expression cassette for the therapeutic gene(s). (cellculturedish.com)
Comparisons of the gene expression profiles of the expanded NK cells and their unstimulated or IL-2-stimulated counterparts showed marked differences. (aacrjournals.org)
Sickle cell disease (SCD) is a group of inherited disorders that results from a genetic mutation in the beta-globin gene forming sickle hemoglobin, Hb S. Hb S polymerizes under deoxygenated conditions in red blood cells giving them an abnormal "sickle" shape. (medicalhomeportal.org)

Scientists showed that a deep surgical margin was resected very close to the tumor site in many neoadjuvant chemotherapy (NAC)-treated oral squamous cell carcinoma patients, suggesting that NAC may lead to local recurrence and poor outcomes. (stemcellsciencenews.com)
The field of investigation of TNT-mediated tumor-stromal, and tumor-tumor, cell-cell communication is gaining momentum. (frontiersin.org)

American Society of Hematology 2020 guidelines for sickle cell disease: management of acute and chronic pain. (medicalhomeportal.org)
American Society of Hematology 2020 guidelines for sickle cell disease: prevention, diagnosis, and treatment of cerebrovascular disease in children and adults. (medicalhomeportal.org)

Viral pneumonia can be diagnosed by isolation and identification of the pathogen through viral culture. (medscape.com)

Lymphoid cells include T cells, B cells, innate lymphoid cells, and natural killer cells. (differencebetween.com)
Nonetheless, when cells rely on anaerobic glycolysis, like cancer cells and certain innate immune cells upon activation, other metabolic pathways sustain succinate levels, including glutamine-dependent anerplerosis to α-ketoglutarate, and eventually citrate by reductive carboxylation. (haematologica.org)
In addition to a role in adaptive immunity, memory CD8+ T cells are involved in innate immunity, being able to become activated and to proliferate under cytokine stimulation (107, 108). (exposed-skin-care.net)

Anatomy48

Organisms15

Diseases27

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment69

Psychiatry and Psychology1

Phenomena and Processes46

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Named Groups4

Health Care9

Stromal cell11

MSCs53

Differentiation19

Rich source of hematopoietic stem1

Vitro10

Differentiate13

CD341

Endothelial cells9

Vivo7

Autologous6

Tissue28

Mesenchymal stromal1

Plastic-adherent1

Tissues16

Mice12

Umbilical cord mesenchymal stem1

Therapies6

Achieved by intravenous1

Adipocytes2

Adult stem2

Self-renewal1

Markers3

Abstract1

Phenotype3

Assays3

Organ4

Clinical11

Pluripotent2

Biology3

HSCs4

Intravenous1

Receptor4

Conclusions1

Myeloid1

Mammalian3

Monolayer2

HSCT1

Adipose2

Morphology2

Humans1

Regeneration1

Macrophages1

Long-term cultures1

Gene7

Tumor2

20202

Pneumonia1

Innate3