• Xeno-free dermal, buccal and gingival fibroblasts can successfully generate iPS with a comparable genotype/phenotype to their xenogenic counterparts. (biomedcentral.com)
  • Fixation and Sirius Red Assay The Sirius Red dye-binding assay for measuring collagen accumulation in gingival fibroblasts was adapted from a prior study completed in osteoblasts [Tullberg-Reinert and Jundt, 1999]. (gpr44.com)
  • To investigate the expression of alkaline phosphatase (ALP) activity in gingival fibroblasts from individuals with chronic periodontitis (CP) and drug-induced gingival hyperplasia (DGH) induced by diphenylhydantoin. (bvsalud.org)
  • Co-culture of skin or synovial fibroblasts with synovial fluid-derived mononuclear cells provided further evidence that these transcriptional differences were functionally significant in an ex vivo setting. (birmingham.ac.uk)
  • A novel 3D spheroid model of rheumatoid arthritis synovial tissue incorporating fibroblasts, endothelial cells, and macrophages. (ucytech.com)
  • Here, we present the engineering of a spheroid-based model of RA synovial tissue which mimics 3D interactions between cells and pro-inflammatory mediators present in the inflamed synovium. (ucytech.com)
  • METHODS: Spheroids were generated by culturing RA fibroblast-like-synoviocytes (RAFLS), human umbilical vein endothelial cells (ECs) and monocyte-derived macrophages in a collagen-based 3D scaffold. (ucytech.com)
  • The fibroblasts grown from frozen stocks have been passaged twice for expansion, prior to becoming plated for experimental remedies at an initial concentration of 50,000 cells per effectively in 6-well plates or 25,000 cells per well in 12-well culture plates. (gpr44.com)
  • Soon after the cells had been grown to full confluence, the fibroblasts had been cultured inside the presence of among the solutions for 7 days, with three media alterations, or 6 days, with two media modifications, every single in the continuous presence of ascorbate, CTGF/CCN2 proteins and anti-CCN2/ CTGF antibodies. (gpr44.com)
  • Platelet-derived growth factors (PDGFs) acting via their tyrosine kinase receptors are major mitogens for many cell types of mesenchymal origin, including fibroblasts and vascular smooth muscle cells (VSMCs)[ 1 - 4 ]. (biomedcentral.com)
  • Their role in enhancing migratory and proliferative responses and extracellular matrix (ECM) synthesis in these cells makes them key regulators of critical biological and pathological functions including tissue remodelling, scarring and fibrosis. (biomedcentral.com)
  • The role of exosomes collected by ultracentrifugation were also analyzed as mediators of p53 expression in cancer cells and APJ expression in fibroblasts. (spandidos-publications.com)
  • APJ expression in fibroblasts co‑cultured with p53‑suppressed colon cancer cells (HCT116 sh p53 cells) was significantly lower than in control colon cancer cells (HCT116 sh control cells). (spandidos-publications.com)
  • In addition, xenografts of HCT116 cells with APJ‑suppressed fibroblasts showed accelerated tumor growth. (spandidos-publications.com)
  • MicroRNA 5703 enriched in exosomes derived from HCT116 sh p53 cells inhibited APJ expression, and inhibition of miR‑5703 diminished APJ suppression in fibroblasts caused by cancer cells. (spandidos-publications.com)
  • Fibroblasts are the main connective tissue cells present in the body. (news-medical.net)
  • As well as being present as fibroblasts, these cells exist in an alternative state, as fibrocytes. (news-medical.net)
  • Fibroblast is the term used to describe these cells when they are in an activated state. (news-medical.net)
  • In some cases, epithelial cells may also produce fibroblasts, a process which is referred to as epithelial-mesenchymal transition (EMT). (news-medical.net)
  • Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
  • Our tissue engineering research focuses on repairing diseased or damaged tissues, incorporating the areas of biomaterials, stem cells, computer modelling and molecular biology. (edu.au)
  • The fundamental concept combines various tissue engineering elements, most often a scaffold as a supporting matrix in combination with living cells and/or bioactive molecules, to form a tissue engineering construct that repairs or regenerates the diseased or damaged tissue or organ. (edu.au)
  • Tissue engineering and regenerative medicine can provide a novel treatment regime based on the use of synthetic biomaterials, which may be constructed into three-dimensional implants and combined with biologics (such as cells and/or bioactive molecules). (edu.au)
  • Unlike many of today's meat alternatives, cultured meat has the potential to create a product that is completely identical to conventional meat, containing exactly the same cells and tissue. (idtechex.com)
  • The heart consists of many kinds of cells, such as heart cells, fibroblasts, and smooth muscle cells. (kcl.ac.uk)
  • They found that cardiac fibroblasts can produce CCL2 and name the cells inflammatory fibroblasts. (kcl.ac.uk)
  • Therefore, the aim of the present study was to identify a regulatory signaling pathway in cardiac inflammation based on the hypothesis that a distinct subset of inflammation-related activated fibroblasts plays crucial roles in the recruitment of inflammatory cells, especially monocytes/macrophages, and in the pathology of heart failure. (kcl.ac.uk)
  • Next steps for this research is to identify the marker for the inflammatory fibroblasts to isolate the cell population and examine the characteristics of the cells to regulate their activity. (kcl.ac.uk)
  • Not only do these wrap pancreatic tumours in a dense, protective layer, they also foster complex relationships with the cancerous cells: some fibroblasts may fuel tumour growth, while other may help to contain its spread. (elifesciences.org)
  • Being able to specifically eliminate pro-cancer fibroblasts requires a better understanding of the factors that shape the role of these cells, and how to identify them. (elifesciences.org)
  • MSCs have multilineage differentiation potential, allowing them to differentiate, for example, into chondrocytes and osteoblasts, the key cells from the two tissues that constitute the osteochondral unit. (hindawi.com)
  • A focus is on the steps of mobilization of cells from the subchondral bone and repair tissue formation, including adherence of the bone marrow clot to the subchondral bone. (hindawi.com)
  • In skin cells we plan to study the contribution of the protein synthesis apparatus to cellular and tissue aging. (uni-ulm.de)
  • Invasion and metastasis of malignant epithelial cells into normal tissues is accompanied by adaptive changes in the mesenchyme-derived supporting stroma of the target organs. (aacrjournals.org)
  • Malignant epithelial cancers, the major cause of cancer morbidity and mortality, arise in organs composed of both epithelial and mesenchyme-derived stromal cells, such as fibroblasts, myofibroblasts, endothelial cells, pericytes, smooth muscle, and hematopoietic cells. (aacrjournals.org)
  • Fibroblasts are cells that help produce connective tissues, such as collagen and elastin, that keep skin firm. (medicalnewstoday.com)
  • Greene said that amniotic stem cells derive their healing power from an ability to develop into any kind of tissue, but he failed to mention that mainstream science does not support his claims. (truthdig.com)
  • For more than half a century, the regenerative possibilities of stem cells - which the body stores to repair damaged tissue and organs and restore blood supply - have tantalized the medical community. (truthdig.com)
  • A subset of orbital fibroblasts in the orbit can differentiate into mature, lipid-accumulating cells or de novo adipogenesis. (medscape.com)
  • Researchers produced pluripotent stem cells from the fibroblast cells in the brain lining of human corpses. (livescience.com)
  • In other words, dead people can yield living cells that can be converted into any cell or tissue in the body. (livescience.com)
  • Mature cells can be made or induced to become immature cells, known as pluripotent stem cells , which have the ability to become any tissue in the body and potentially can replace cells destroyed by disease or injury. (livescience.com)
  • Fibroblasts are the most common cells of connective tissue in animals, and they synthesize the extracellular matrix, the complex scaffolding between cells. (livescience.com)
  • Cadaver-collected fibroblasts can be reprogrammed into induced pluripotent stem cells using chemicals known as growth factors that are linked with stem cell activity. (livescience.com)
  • Now scientists have taken fibroblasts from the scalps and the brain linings of 146 human brain donors and grown induced pluripotent stem cells from them as well. (livescience.com)
  • Studying how induced pluripotent stem cells develop into various tissues could also shed light on disorders that are due to malfunctions in development. (livescience.com)
  • cells in the outer layer of the skin called keratinocytes, and structural cells called fibroblasts. (medlineplus.gov)
  • I-cell disease fibroblasts were subsequently discovered to be able to internalize and use lysosomal enzymes produced by normal cells, whereas normal or other lysosomal disease fibroblasts were incapable of internalizing lysosomal enzymes secreted by the I-cell disease fibroblasts. (medscape.com)
  • These are observed in cells of mesenchymal origin, especially fibroblasts. (medscape.com)
  • however, significant vacuolization is present in the heart's connective tissue cells of the heart valves. (medscape.com)
  • The initial tissue reaction to infection is a focal, intensely suppurative necrosis consisting largely of accumulations of polymorphonuclear leukocytes, followed by invasion of macrophages, epithelioid cells, and lymphocytes. (cdc.gov)
  • Suppurative lesions become granulomatous, and histopathological examination of the granulomas shows a central necrotic, sometimes caseating, zone surrounded by a layer of epithelioid cells, multinucleated giant cells, and fibroblasts in a radial arrangement, typical of other granulomatous conditions such as tuberculosis and sarcoidosis. (cdc.gov)
  • Synovial fibroblasts were isolated via enzymatic processing from synovial tissues obtained from patients with RA or Osteoarthritis (OA). (biomedcentral.com)
  • A mitogenic growth factor activity was found in pituitary extracts by Armelin in 1973 and further work by Gospodarowicz as reported in 1974 described a more defined isolation of proteins from cow brain extract which, when tested in a bioassay that caused fibroblasts to proliferate, led these investigators to apply the name "fibroblast growth factor. (wikipedia.org)
  • These independently discovered proteins were eventually demonstrated to be the same sets of molecules, namely FGF1, HBGF-1 and ECGF-1 were all the same acidic fibroblast growth factor described by Gospodarowicz, et al. (wikipedia.org)
  • Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family. (bvsalud.org)
  • collagen and other extracellular proteins in various tissues are overproduced. (msdmanuals.com)
  • Interestingly, the transcriptional response of skin fibroblasts to IL-4 converged with that of TNF-alpha-treated synovial fibroblasts, suggesting resident tissue fibroblasts and their blood-borne precursors may be imprinted by inflammatory cytokines that are characteristic of different tissues. (birmingham.ac.uk)
  • Upon activation within the joint's inflammatory milieu, they gain a transformed phenotype and produce pro-inflammatory cytokines (mainly IL-6) and tissue destructive enzymes [ 1 ]. (biomedcentral.com)
  • CAFs and normal fibroblasts differ with respect to the expression of various markers, such as fibrotic markers, growth factors, chemokines and cytokines ( 9 ). (spandidos-publications.com)
  • In murine and human lung fibroblasts, DE induced the release of AREG and inflammatory cytokines. (cdc.gov)
  • The fibroblasts then produce hyaluronan and the cytokines interleukin (IL)-1 beta, IL-6, IL-8, IL-16, tumor necrosis factor (TNF) alpha, and CD4 ligand. (medscape.com)
  • The cytokines and hyaluronan expand the orbital tissue, muscles, and fat by drawing water into these tissues. (medscape.com)
  • 6). Lung tissue, bronchoalveolar lavage fluid (BALF) and plasma were analyzed for cellular inflammation, cytokines, immunoglobulin E and heme oxygenase-1 (HO-1) expression. (cdc.gov)
  • Synovial fibroblasts are key players in the pathogenesis of Rheumatoid Arthritis (RA) and potentially attractive treatment targets. (biomedcentral.com)
  • Synovial fibroblasts (passages 2-4) were stimulated with TNF-α (10 ng/ml) only on day 1. (biomedcentral.com)
  • In stark contrast, synovial fibroblasts displayed a remarkably more sustained response to TNF-α. (biomedcentral.com)
  • The levels of IL-6 mRNA induced by TNF-α in synovial fibroblasts were substantially higher compared to human Mϕ, suggesting that within the joint microenvironment, synovial fibroblasts and not Mϕ are the main source of IL-6. (biomedcentral.com)
  • By adding the supernatants from 96 h TNF-α-stimulated fibroblast cultures on unstimulated synovial fibroblasts, a similar robust induction of IL-6 mRNA was observed, suggesting that there is a TNF-α-induced soluble factor that mediates the sustained response. (biomedcentral.com)
  • Interestingly, there was no difference between OA- and RA-derived synovial fibroblasts in their response to TNF-α. (biomedcentral.com)
  • In contrast to human Mϕ, synovial fibroblasts display a sustained inflammatory and tissue destructive response to TNF-α. (biomedcentral.com)
  • Our observations suggest that synovial fibroblasts may lack the homeostatic mechanisms that control and terminate the effects of TNF-α on human Mϕ [ 2 ]. (biomedcentral.com)
  • To support this hypothesis, further investigation is needed at the level of proximal and distal TNF-α signaling events and at the level of epigenetic regulation of TNF-α-target genes in synovial fibroblasts. (biomedcentral.com)
  • iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
  • The strategy is compared against both manual peak picking and industry standard automated peak picking on a set of mass spectra obtained after tryptic in gel digestion of 2D-gel samples from human fetal fibroblasts. (lu.se)
  • PDGF-AA is a potent mitogen for cardiac fibroblasts and has been shown to be critical in lung alveolar myofibroblast development and alveogenesis[ 13 , 14 ]. (biomedcentral.com)
  • A team of researchers, led by Professor Kinya Otsu, showcase how a distinct subset of cardiac fibroblasts become activated by pressure overload and attract monocytes to the heart. (kcl.ac.uk)
  • They then examined the mRNA expression pattern at a single cell level using so-called single-cell RNA sequencing analysis and found a subset of cardiac fibroblasts highly expressed genes activated by the transcription factor NF-kB and CCL2 mRNA. (kcl.ac.uk)
  • In the mouse which did not express the NF-kB activator IKKb in cardiac fibroblasts, pressure overload induced less monocyte recruitment and did not induce heart failure. (kcl.ac.uk)
  • The adult heart contains many resident cardiac fibroblasts, which play important roles in maintaining tissue architecture. (kcl.ac.uk)
  • In response to tissue injury or stress, the resident cardiac fibroblasts undergo a transition to activated myofibroblasts, a highly synthetic cell type that induces the genes encoding smooth muscle α actin (ACTA2) and collagen. (kcl.ac.uk)
  • Our results provide insight into the mechanisms underlying cardiac inflammation and fibroblast-mediated inflammatory responses that could be therapeutically targeted to treat heart failure. (kcl.ac.uk)
  • To conclude, we found that a subset of cardiac fibroblasts involved in cardiac inflammation under stress. (kcl.ac.uk)
  • The cardiac fibroblast might therefore be a therapeutic target to treat patients with heart failure. (kcl.ac.uk)
  • CONCLUSION: We present a novel spheroid based model consisting of RAFLS, ECs and macrophages that reflects the RA synovial tissue microenvironment. (ucytech.com)
  • Thus, the APJ system in fibroblasts in the tumor microenvironment may be a promising therapeutic target. (spandidos-publications.com)
  • We aim to gain a better understanding of the relationship between T cell functions and their physical microenvironment by 3D printing hydrogels as a model for the soft tissues and organs in our bodies. (edu.au)
  • Stromal fibroblasts are a dominant but heterogenous population within the tumor microenvironment and therapeutic targeting of stromal subsets may have therapeutic utility. (elifesciences.org)
  • The plasticity and heterogeneity of fibroblasts in the tumor microenvironment of pancreatic ductal adenocarcinoma (PDAC) has emerged as a key factor in determining tumor growth and therapeutic response. (elifesciences.org)
  • Although myofibroblasts have been considered the only phenotype of activated fibroblasts, distinct subsets of fibroblasts, namely inflammatory fibroblasts and myofibroblasts, have been reported in arthritis and pancreatic cancer. (kcl.ac.uk)
  • Tumor-proximal fibroblasts comprise large populations of myofibroblasts, strongly expressed podoplanin, and were enriched for Wnt ligand signaling. (elifesciences.org)
  • For example, certain fibroblasts close to the tumour resemble the myofibroblasts present in healing wounds, while those at the periphery show signs of being involved in inflammation. (elifesciences.org)
  • Human FGF18 is involved in cell development and morphogenesis in various tissues including cartilage. (wikipedia.org)
  • Different versions (isoforms) of the USB1 enzyme are produced in different tissues, where they play various roles. (medlineplus.gov)
  • Up to 10 isoforms can be present in different amounts in different tissues. (lu.se)
  • We investigated the extent to which fibroblasts isolated from diverse tissues differ in their capacity to modulate inflammation by comparing the global gene expression profiles of cultured human fibroblasts from skin, acute and chronically inflamed synovium, lymph node and tonsil. (birmingham.ac.uk)
  • and colon fibroblasts, CCD‑18Co), resected human tissue samples of colorectal cancers, and immune‑deficient nude mouse xenograft models. (spandidos-publications.com)
  • Transient transfection of promoter-chloramphenicol O-acetyltransferase reporter constructs into primary human connective tissue fibroblasts shows that a 904 bp fragment that hybridizes to a murine TIMP-1 promoter fragment contains a functional promoter. (uea.ac.uk)
  • If Quercetin & Fisetin decrease collagen concentration in human fibroblasts, would that have a negative effect on bone density, skin quality, & wound healing? (news-medical.net)
  • The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
  • Fibroblast recellularization of primary human lung mesenchymal matrix scaffolds and wound closure was inhibited by DE and enhanced with recombinant AREG alone. (cdc.gov)
  • Human FLS were prepared from synovial tissues obtained as discarded specimens following joint arthroplasty. (acrabstracts.org)
  • A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
  • Past research showed this same process could be carried out with so-called fibroblasts taken from the skin of human cadavers. (livescience.com)
  • Wiktor TJ, Plotkin SA, Koprowski H. Development and clinical trials of the new human rabies vaccine of tissue culture (human diploid cell) origin. (cdc.gov)
  • This suggests that periodontal inflammation and diphenylhydantoin may influence ALP expression and human gingival fibroblast expansion. (bvsalud.org)
  • Directly Converted Human Fibroblasts Mature to Neurons and Show Long-Term Survival in Adult Rodent Hippocampus. (lu.se)
  • In the extremities, chronic inflammation and fibrosis of the synovial membrane and surfaces and periarticular soft tissues occur. (msdmanuals.com)
  • Conversely, fibroblasts sometimes undergo mesenchymal-epithelial transition (MET) to produce epithelia, a process that is seen in development, tissue repair and tumor growth. (news-medical.net)
  • Fibroblastic connective tissue nevus (FCTN): First described in 2012, FCTN is a mesenchymal lesion with a rare incidence. (medscape.com)
  • Progesterone increases the production of tissue inhibitor of metalloproteinases-2 in rabbit uterine cervical fibroblasts. (ox.ac.uk)
  • Rabbit uterine cervical fibroblasts in culture produces tissue inhibitor of metalloproteinases (TIMP)-1 and TIMP-2. (ox.ac.uk)
  • Thus, some are sequestered in the extracellular matrix of tissues that contains heparan sulfate proteoglycans and are released locally upon injury or tissue remodeling. (wikipedia.org)
  • Extracellular matrix scaffolds derived from porcine small intestinal submucosa (SIS-ECM) have been shown to promote the formation of site-specific tissue in a number of preclinical animal studies. (researchgate.net)
  • A fibroblast is a type of cell that is responsible for making the extracellular matrix and collagen. (news-medical.net)
  • Together, this extracellular matrix and collagen form the structural framework of tissues in animals and plays an important role in tissue repair. (news-medical.net)
  • They achieve this by secreting extracellular matrix precursors required for formation of the connective tissue and various fibres. (news-medical.net)
  • The synovial lining tissue consists of fibroblast-like synoviocytes (FLS) and monocyte-derived macrophage-like synoviocytes (MLS) within a self-built meshwork of dense extracellular matrix (ECM) components. (acrabstracts.org)
  • In humans, 23 members of the FGF family have been identified, all of which are structurally related signaling molecules: Members FGF1 through FGF10 all bind fibroblast growth factor receptors (FGFRs). (wikipedia.org)
  • Immunoglobulins against insulin-like growth factor 1 (IGF-1) receptors activate signaling in the orbital fibroblasts. (medscape.com)
  • White adipose tissue (WAT) has key metabolic and endocrine functions and plays a role in regulating energy homeostasis and insulin sensitivity. (springer.com)
  • Conversely, brown adipose tissue (BAT) and browning of WAT represent potential therapeutic approaches, since dysfunctional white adipocyte-induced lipid overspill can be halted by BAT/browning-mediated oxidative anti-lipotoxic effects. (springer.com)
  • Here, we summarise the mechanisms contributing to adipose tissue (AT) plasticity and function including characteristics and cellular complexity of the various adipose depots and we discuss recent insights into AT origins, identification of adipose precursors, pathophysiological regulation of adipogenesis and its relation to WAT/BAT expandability in obesity and its associated comorbidities. (springer.com)
  • White adipose tissue (WAT) plays a key homeostatic role, not only by ensuring efficient energy storage but also by its quick mobilisation (lipids) to ensure peripheral demands. (springer.com)
  • The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
  • There is also evidence that the deleterious effects mediated by dysfunctional white adipocyte-induced lipid overspill can be halted by the pro-oxidative anti-lipotoxic effects mediated by brown adipose tissue (BAT) activation. (springer.com)
  • The apelin‑apelin receptor (APJ) system has been implicated in suppressing fibroblast‑to‑myofibroblast transition in non‑neoplastic organ fibrosis. (spandidos-publications.com)
  • Single-cell deconvolution of fibroblast heterogeneity in mouse pulmonary fibrosis. (nature.com)
  • Defining the activated fibroblast population in lung fibrosis using single-cell sequencing. (nature.com)
  • In this study, we used PDGFR-specific neutralising antibodies to dissect out receptor-specific signalling events in fibroblasts in vitro , to further understand key cellular processes involved in wound healing and tissue repair. (biomedcentral.com)
  • In vitro , PDGFR isoforms have been shown to be potent activators of fibroblast proliferation, migration and survival[ 10 ]. (biomedcentral.com)
  • This tempted us to study the dynamics of synovial tissue modelling under steady state and inflammatory conditions using a three-dimensional in-vitro model of the synovial tissue. (acrabstracts.org)
  • It concentrates in phagocytes and fibroblasts, as demonstrated by in vitro incubation techniques. (medscape.com)
  • Our work aims to develop optimal tissue engineered constructs for the repair and regeneration of different types of musculoskeletal tissues, including bone, cartilage and tendon. (edu.au)
  • 43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
  • Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
  • MG-63, a cell that has fibroblast morphology isolated from the bone of a White, 14-year-old male patient with osteosarcoma. (atcc.org)
  • Mobilized pluripotent MSCs from the subchondral bone migrate into the defect filled with the clot, differentiate into chondrocytes and osteoblasts, and form a repair tissue over time. (hindawi.com)
  • Over time, they form a fibrocartilaginous repair tissue in the defect and close the connection with the subchondral bone. (hindawi.com)
  • Among different types of scaffolds, polymeric hydrogel scaffolds have received considerable attention because of their biocompatibility and structural similarity to native tissues. (mdpi.com)
  • The primary function of fibroblasts is the maintenance of structural integrity within the connective tissue. (news-medical.net)
  • Tissue exhibits unique mechanical, biochemical and structural properties, with discrete and continuous changes in cellular and extracellular composition that defines intricate channels, chambers and interfaces. (edu.au)
  • 48,49 In addition, cyclic mechanical strain enhances the function and development of engineered tissues by improving the production of collagen and elastin. (researchgate.net)
  • Challenges in organ transplantation such as high organ demand and biocompatibility issues have led scientists in the field of tissue engineering and regenerative medicine to work on the use of scaffolds as an alternative to transplantation. (mdpi.com)
  • Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades. (researchgate.net)
  • Two-dimensional (2D) settings present very limited physiological proximity as they cannot recapitulate cell-cell and cell-matrix interactions occurring in the three-dimensional (3D) tissue compartment. (ucytech.com)
  • APJ suppression from a specific microRNA in cancer cell‑derived exosomes induced CAF‑like properties in fibroblasts. (spandidos-publications.com)
  • Fibrocyte refers to the less active state, when the cell is involved in tissue maintenance and metabolism. (news-medical.net)
  • Some researchers use the term fibroblast for both cellular states but the term "blast" usually refers to a stem cell or a cell's activated state. (news-medical.net)
  • Here the authors use innovative approaches to combine spatial profiling with single cell transcriptomics to define tumor-proximal populations of fibroblasts that predict clinical outcome. (elifesciences.org)
  • This may be due, in part, to a type of cell called fibroblasts. (elifesciences.org)
  • The prepared chitin/gelatin membranes with or without GlcNAc were characterized by mechanical, swelling, enzymatic degradation, thermal and growth of NIH/3T3 fibroblast cell studies. (amrita.edu)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • Early enzymologic studies showed that cultured fibroblasts from patients with I-cell disease were deficient in numerous lysosomal enzymes. (medscape.com)
  • Other sites of abnormal cell vacuolization include the renal glomerular podocytes and in the fibroblasts of the liver's periportal spaces. (medscape.com)
  • The tissue culture fluid is harvested and filtered to remove cell debris. (cdc.gov)
  • Using an enzymatic digestion procedure, gingival cell suspensions containing ALP-positive fibroblasts were prepared without affecting ALP activity. (bvsalud.org)
  • Little cell proliferation was observed for fibroblasts from CP and DGH as compared to NG. (bvsalud.org)
  • The responses of these fibroblasts to TNF-alpha, IFN-gamma and IL-4 stimulation were markedly different, as revealed by hierarchical cluster analysis and principal component analysis. (birmingham.ac.uk)
  • This pressing need has led to the rise of 'tissue engineering and regenerative medicine', a multidisclinary field which aims to induce the body's natural regenerative abilities and produce functional substitutes of biological tissue for clinical use. (edu.au)
  • Neutrophils and activated macrophages (CD11c+ CD11bhi) persisted after recovery in lung tissues of anti-AREG-treated mice. (cdc.gov)
  • approaches simulating RA synovial tissue are crucial in preclinical and translational research to evaluate novel diagnostic and/or therapeutic markers. (ucytech.com)
  • Visible and infrared light have been shown to affect positive therapeutic benefits to living tissues and organisms on a cellular level. (nutrimedical.com)
  • This proved to be biologically relevant, as TNF-alpha induced the secretion of different patterns and amounts of IL-6, IL-8 and CCL2 (MCP-1) in the two fibroblast types. (birmingham.ac.uk)
  • Isoforms that lack the n-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. (lu.se)
  • Prior to joining Surrey, her research centred around pulmonary hypertension and connective tissue diseases. (surrey.ac.uk)
  • Our findings suggest that fine PM enhances allergic inflammatory response in pulmonary tissue through mechanisms involving increased oxidative stress. (cdc.gov)
  • APJ‑suppressed fibroblasts treated with an antagonist or small interfering RNA showed myofibroblast‑like properties, including increased proliferation and migratory abilities, via accelerated phosphorylation of Sma‑ and Mad‑related protein 2/3 (Smad2/3). (spandidos-publications.com)
  • The expression of ALP is increased in CP and DGH, and fibroblasts in CP and DGH show low proliferation. (bvsalud.org)
  • Specifically, elevated expression of HIF-1a and podoplanin predicted worse outcome while inflammatory gene expression correlated with increased survival, suggesting future interventions targeting proximal fibroblast populations to mitigate against PDAC. (elifesciences.org)
  • They mapped out the location of individual fibroblasts in the vicinity of the tumour and analysed their gene activity. (elifesciences.org)
  • FGF1 is also known as acidic fibroblast growth factor, and FGF2 is also known as basic fibroblast growth factor. (wikipedia.org)
  • In 1975, they further fractionated the extract using acidic and basic pH and isolated two slightly different forms that were named "acidic fibroblast growth factor" (FGF1) and "basic fibroblast growth factor" (FGF2). (wikipedia.org)
  • while FGF2, HBGF-2, and ECGF-2 were all the same basic fibroblast growth factor. (wikipedia.org)
  • Various genetic abnormalities such as wingless and int-1 (Wnt) signaling, tissue growth factor beta (TGFβ) signaling, or TP53 signaling have been known in the carcinogenesis of colorectal cancer ( 2 , 3 ). (spandidos-publications.com)
  • Bovine fibroblast growth factor 21 (FGF21) ELISA kit is Available at Gentaur Genprice with the fastest delivery. (joplink.net)