• Jurkat cell lysate and human spleen tissue. (abcam.cn)
  • IP: Jurkat whole cell lysate. (abcam.cn)
  • IP: HeLa cell lysate. (abcam.co.jp)
  • Lysate (reverse phase) arrays contain complex samples, such as cell or tissue lysates, that are printed on an array surface and interrogated with antibodies. (tovi.io)
  • Lysate was prepared from a human breast cancer tissue sample. (tovi.io)
  • Detection:11265-1-AP 1:300) with Jurkat cells lysate 4000ug. (ptglab.com)
  • WB analysis of A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH3T3 (6), PC-12 (7) and Cos7 (8) cell lysate using GTX60552 ASS1 antibody [2C10]. (genetex.com)
  • Western blot: 10 µg of Jurkat cell lysate stained with ARG66751 anti-CD5 antibody [SQab20184] at 1:1000 dilution. (arigobio.cn)
  • Lysate from Jurkat cells was probed with anti-Fli-1 (clone G146-254) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). (bdbiosciences.com)
  • Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
  • human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 10x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 40x lens). (ptglab.com)
  • U-937 cells were subjected to SDS PAGE followed by western blot with 15114-1-AP (FKBP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15114-1-AP (FKBP1B antibody) at dilution of 1:200 (under 10x lens). (ptglab.com)
  • Hybridoma technology, specifically used for generating immortalised antibody-producing B cell lines, where an antibody-producing B cell is fused with a myeloma (B cell cancer) cell. (wikipedia.org)
  • The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
  • C) Jurkat cells were labeled with an anti-ceramide C16 antibody and a secondary Alexa 647 labeled antibody. (uni-wuerzburg.de)
  • Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody. (novusbio.com)
  • Immunocytochemistry/ Immunofluorescence: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1. (novusbio.com)
  • Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Jurkat cells, using anti-SAMHD1 antibody,(Red), compared to a nonspecific negative control antibody,(Blue). (novusbio.com)
  • Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. (novusbio.com)
  • Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody. (novusbio.com)
  • Positive control samples (Jurkat cell pellets) and antibody ( AbFlex ® H3K9ac ) are included in the module for user optimization of the Low Cell ChIP protocol. (activemotif.com)
  • IHC-P analysis of cervical cancer tissue using GTX60552 ASS1 antibody [2C10]. (genetex.com)
  • Mer appearance was dependant on confocal imaging of Jurkat cells stably expressing shRNA aimed against GFP (shControl, non-targeting control) or Mer (shMer1A) pursuing immunofluorescence staining for Mer, and WT Jurkat cells stained just with supplementary antibody (DyLight549) serve to show insufficient nonspecific binding. (greatlakeshighereducationnow.com)
  • Merged pictures of Mer (Immunoblot recognition of Mer in WT, shControl, and shMer1A Jurkat cells (using the same anti-MerTK antibody) acts as a guide for confocal pictures and demonstrates enough shRNA-mediated knockdown of AKT inhibitor VIII (AKTI-1/2) Mer. (greatlakeshighereducationnow.com)
  • The tissue section was stained with ARG66751 anti-CD5 antibody [SQab20184] at 18°C - 25°C for 30 minutes. (arigobio.cn)
  • OriGene offers a range of tools to analyze CD markers including cDNA clones, antibodies, overexpression cell lysates and more. (origene.com)
  • WB: 293T, HeLa, Jurkat and K562 cell lysates. (abcam.co.jp)
  • lysates were prepared from these and from a Jurkat T cell control. (tovi.io)
  • Lysates from control (lanes 1-3) and camptothecin treated Jurkat cells (lanes 4-6) were probed with anti-human caspase-8 (clone 4-1-20, Cat. (bdbiosciences.com)
  • A) TAM receptor appearance was discovered by traditional western blot of whole-cell lysates gathered from three individual cell linesHeLa (cervical carcinoma), Jurkat (T-ALL), and NB4 (AML FAB M3). (greatlakeshighereducationnow.com)
  • Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
  • Cells were labeled with antibodies against ß-tubulin (green) and Alexa 647 phalloidin (magenta). (uni-wuerzburg.de)
  • In contrast to most previous studies in which agonistic anti-TCR/CD3 and anti-CD28 antibodies were employed, here we have investigated the contribution of CD28 to T cell activation under physiological conditions of antigen presentation. (ox.ac.uk)
  • In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell-engager (BiTE) antibodies. (aacrjournals.org)
  • To track VHH in patients, animal models, and cell cultures, specific anti-VHH antibodies are required. (genscript.com)
  • GenScript MonoRab™ Rabbit Anti-VHH antibodies show ultra-high specificity for VHH-based CAR T cells. (genscript.com)
  • More recently, these cells have been exploited in imaging studies to identify key players in immunological synapse (IS) assembly in superantigen-specific conjugates and to track the dynamics of signaling molecules on glass surfaces coated with activating anti-CD3 antibodies. (ox.ac.uk)
  • The origins of some immortal cell lines, for example HeLa human cells, are from naturally occurring cancers. (wikipedia.org)
  • HeLa, the first immortal human cell line on record to be successfully isolated and proliferated by a laboratory, was taken from Henrietta Lacks (without informed consent) in 1951 at Johns Hopkins Hospital in Baltimore, Maryland. (wikipedia.org)
  • Major examples include human HeLa, the line is derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951. (wikipedia.org)
  • HeLa cells - a widely used human cell line isolated from cervical cancer patient Henrietta Lacks. (wikipedia.org)
  • Flow Cyt (intra): HeLa and Jurkat cells. (abcam.com)
  • Flow Cyt (intra): HeLa cells. (abcam.co.jp)
  • A549 cells - derived from a cancer patient lung tumor. (wikipedia.org)
  • ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. (reliatech.de)
  • Finally, in vivo studies demonstrated that hyperoside significantly reduced tumor size, inhibited PD-L1 and RNF8 expression, and induced apoptosis in tumor tissues of a subcutaneous mouse model. (bvsalud.org)
  • While aberrant DNA methylation is a characteristic feature of tumor cells, our knowledge of how these DNA methylation patterns are established and maintained is limited. (biomedcentral.com)
  • Here, we report that in T cell acute lymphoblastic leukemia (T-ALL) the MYC oncogene controls the expression of TET1 and TET2 to maintain 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) patterns, which is associated with tumor cell-specific gene expression. (biomedcentral.com)
  • Knockdown of TET1 or ectopic expression of TET2 in T-ALL was associated with genome-wide changes in 5mC and 5hmC enrichment and decreased cell proliferation, suggesting a tumor promoting function of TET1, and a tumor suppressing role for TET2. (biomedcentral.com)
  • Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. (aacrjournals.org)
  • Notably, intratumoral injection of this cBiTE-expressing adenovirus increased the persistence and accumulation of tumor-infiltrating T cells in vivo , compared with the parental virus lacking such effects. (aacrjournals.org)
  • ICC/IF: HepG2 cells and A549 (SLC2A1 knockout A549 cells used as a negative control) cells. (abcam.com)
  • Under AAS culture conditions, the cell killing effect of GEF was synergistically pronounced in the EGFR-expressing cell lines, namely, CAL 27, Detroit 562, A549 and PANC-1 cells compared with those treated with either GEF or AAS alone. (spandidos-publications.com)
  • Cancer occurs when a somatic cell which normally cannot divide undergoes mutations which cause de-regulation of the normal cell cycle controls leading to uncontrolled proliferation. (wikipedia.org)
  • Like BAFF, APRIL also stimulates the proliferation of B and T cells. (reliatech.de)
  • Albright CD, Klem E, Shah AA, Gallagher P. Breast cancer cell-targeted oxidative stress: Enhancement of cancer cell uptake of conjugated linoleic acid, activation of p53, and inhibition of proliferation. (raw-milk-facts.com)
  • Jurkat T cells encapsulated with a double layer of PSS-GNR/PAH (PSS-GNR/PAH@Jurkat) showed the highest rate of cell proliferation when compared to 24-h encapsulated cells. (springer.com)
  • The knockdown of expression in ALK-mutated cells, but also in cell lines overexpressing the wildtype protein, led to a marked decrease of cell proliferation. (nsjbio.com)
  • The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. (arigobio.cn)
  • May act as a receptor in regulating T-cell proliferation. (arigobio.cn)
  • In cell coculture assays, ICOVIR-15K-cBiTE-mediated oncolysis resulted in robust T-cell activation, proliferation, and bystander cell-mediated cytotoxicity. (aacrjournals.org)
  • Extensive lymphocyte severe infections is a massive loss of lymphocytes, den- apoptosis has also occurred in humans and animals infect- dritic cells, gastrointestial epithelial cells, and other cell ed with several exotic agents, including Bacillus anthracis , types through apoptosis, or programmed cell death. (cdc.gov)
  • Prevention of lymphocyte apoptosis, same process by which these cell populations are regulat- through either genetic modification of the host or treatment ed during normal health ( 1 , 2 ). (cdc.gov)
  • acquired defects in the control of apoptosis may lead to a Apoptosis, or programmed cell death, is the method variety of disease states. (cdc.gov)
  • For example, excessive inhibition by which tissue remodeling takes place during normal of apoptosis is an underlying mechanism of cancer, while growth and development and the physiologic mechanism an inappropriate increase is seen in some neurodegenera- by which labile cell populations such as gastrointestinal tive diseases and other conditions. (cdc.gov)
  • Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g. (bdbiosciences.com)
  • METHODS: 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl Tetrazolium Bromide (MTT), transwell, and flow cytometry assays were used to detect PCa cell growth, invasion, and cell apoptosis. (bvsalud.org)
  • RESULTS: Hyperoside inhibited PCa cell growth, invasion, and cell cycle and induced cell apoptosis. (bvsalud.org)
  • Imidazo[1,2-b]pyrazole-7-carboxamides induce apoptosis in human leukemia cells at nanomolar concentrations. (celljournal.org)
  • Riedl S, Leber R, Rinner B, Schaider H, Lohner K, Zweytick D. Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine. (celljournal.org)
  • Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
  • Real-life exposure to Fusarium toxins deoxynivalenol and zearalenone triggers apoptosis and activates NLRP3 inflammasome in bovine primary theca cells. (inrae.fr)
  • To evaluate whether an immunotherapeutic vaccine could elicit skin-based memory T cells we studied skin biopsies and HSV-2-reactive CD4+ T cells from peripheral blood mononuclear cells (PBMCs) by T-cell receptor (TCR) sequencing before and after vaccination with a replication-incompetent whole virus HSV-2 vaccine candidate (HSV529). (medrxiv.org)
  • The representation of HSV-2-reactive CD4+ T cell sequences from PBMCs increased from a median of 0.03% (range 0-0.09%) to 0.6% (range 0-1.3%) of the total skin TCR repertoire after the first vaccine dose. (medrxiv.org)
  • We found sustained expansion after vaccination in unique, skin-based T-cell clonotypes that were not detected in HSV-2-reactive CD4+ T cells isolated from PBMCs. (medrxiv.org)
  • In the present study, the anticancer activity of pleurocidin-like peptide WF3 isoform X2 (AMP-WF3), from the Poecilia Mexicana fish, against leukemic cell line Jurkat was evaluated, and the cytotoxicity compared with the effects on normal cells, including peripheral blood mononuclear cells (PBMCs) and human dermal fibroblast (HDF) cells. (celljournal.org)
  • IC50)=50 μM], while normal cells (PBMCs and HDF) were less susceptible. (celljournal.org)
  • Flow cytometry verified that the apoptotic activity of AMP-WF3 on Jurkat cells was significantly higher than that of HDF and PBMCs. (celljournal.org)
  • In contrast, the changes in P21, P53, and BCL-2 genes differed in PBMCs and HDF cells. (celljournal.org)
  • In HDF cells, simultaneous increase of P21, P53, and BCL-2, and in PBMCs, only the increase of BCL-2 was observed. (celljournal.org)
  • PBMCs, Jurkat cells) with version 2 of our 3' GEX assay (3'v2). (10xgenomics.com)
  • Agatha G, Voigt A, Kauf E, Zintl F. Conjugated linoleic acid modulation of cell membrane in leukemia cells. (raw-milk-facts.com)
  • The transport of serum amino acids into cells is an active process that is facilitated by plasma membrane-localized amino acid transporters. (spandidos-publications.com)
  • Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. (arigobio.cn)
  • Calcium channels made with the CACNA1S subunit are located in the outer membrane of muscle cells, so they can transmit electrical signals from the cell surface to inside the cell. (medlineplus.gov)
  • RYR1 channels are located in the membrane of a structure inside the cell that stores calcium ions. (medlineplus.gov)
  • Yet, to date allogeneic HCT is facing three major challenges: First, adequate immune reconstitution and control of infections, second, overreaching immune responses that can result in life-threatening graft-versus-host disease and, third, impaired immune effector functions that are not sufficient to control the underlying disease, e.g. eliminating remaining leukemic cells. (uni-wuerzburg.de)
  • These studies demonstrated that a combination of three different osmolytes including sugar, sugar alcohol and amino acids/proteins could stabilize Jurkat cells and mesenchymal stromal cells (MSCs) during freezing. (nature.com)
  • The cells can therefore be grown for prolonged periods in vitro. (wikipedia.org)
  • Other immortalised cell lines are the in vitro equivalent of cancerous cells. (wikipedia.org)
  • Immortalised cell lines have undergone similar mutations allowing a cell type which would normally not be able to divide to be proliferated in vitro. (wikipedia.org)
  • Immortalised cell lines find use in biotechnology, where they are a cost-effective way of growing cells similar to those found in a multicellular organism in vitro. (wikipedia.org)
  • Decreased PD-L1 levels contributed to induced immunity in Jurkat cells in vitro. (bvsalud.org)
  • Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. (bdbiosciences.com)
  • Our studies seek to recapitulate, in vitro, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function. (elsevierpure.com)
  • Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. (nsjbio.com)
  • Gut-associated lymphoid tissue is a major target and reservoir of human immunodeficiency virus (HIV)-infected T-cells. (elsevierpure.com)
  • DNA standards and GAPDH-2 primers were used to analyze DNA from Fixed Jurkat Cell Pellet samples (equivalent to 100,000 Jurkat cells). (activemotif.com)
  • Nuclear extracts were prepared from 100,000 cells for each cell line and condition, and incubated with 2.5 μl of transposase (Illumina) in a 50 μl reaction for 30 min at 37 °C. Transposase-fragmented DNA was purified, and the library was amplified by PCR. (chip-atlas.org)
  • We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
  • The cells are used for a wide variety of purposes, from testing toxicity of compounds or drugs to production of eukaryotic proteins. (wikipedia.org)
  • Polypeptides, especially star polypeptides, as a unique kind of biological macromolecules have broad applications in biomedical fields such as drug release, gene delivery, tissue engineering, and regenerative medicines due to their close structural similarity to naturally occurring peptides and proteins, biocompatibility, and amino acid functionality. (bvsalud.org)
  • The Low Cell ChIP Kit is robust enough to work with both high abundance histone modifications and low abundance transcription factor proteins. (activemotif.com)
  • In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. (lu.se)
  • An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. (wikipedia.org)
  • After Jurkat T cells were encapsulated with poly(allylamine hydrochloride) (PAH) and/or PSS-GNRs or PSS, most cells survived and could proliferate. (springer.com)
  • Chimeric antigen receptor (CAR) T-cell therapy is a rapidly growing therapy for the treatment of cancer 1 . (nature.com)
  • The efficiency and magnitude of T cell responses are influenced by ligation of the co-stimulatory receptor CD28 by B7 molecules expressed on antigen-presenting cells (APC). (ox.ac.uk)
  • Jurkat T cells and primary T cells from TCR-transgenic mice stimulated with superantigen and antigen, respectively, presented by B7-expressing APC were utilized. (ox.ac.uk)
  • Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)
  • Bergamo P, Luongo D, Maurano F, Rossi M. Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T-cells. (raw-milk-facts.com)
  • Western blotting revealed that TMP decreased the protein expression of TNF superfamily receptor 1 (TNFR1), inhibitor κB-α (IκB-α), and NF-κB p65 in spinal cord tissues. (bvsalud.org)
  • Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene. (jamanetwork.com)
  • Clonal rearrangements of T-cell receptor genes in mycosis fungoides and dermatopathic lymphadenopathy. (jamanetwork.com)
  • Detection of clonal T-cell receptor gamma gene rearrangements in early mycosis fungoides/Sézary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE). (jamanetwork.com)
  • Bottaro MBerti EBiondi AMigone NCrosti L Heteroduplex analysis of T-cell receptor gamma gene rearrangements for diagnosis and monitoring of cutaneous T-cell lymphomas. (jamanetwork.com)
  • Tok JSzaboles MJSilvers DNZhong JMatsushima AY Detection of a clonal T-cell receptor gamma chain gene rearrangements by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE) in archival specimens from patients with early cutaneous T-cell lymphoma: correlation of histologic findings with PCR/DGGE. (jamanetwork.com)
  • Langerak AWScczepanski Tvan der Burg MWolvers-Tettero ILvan Dongen JJ Heteroduplex PCR analysis of rearranged T-cell receptor genes for clonality assessment in suspect T-cell proliferations. (jamanetwork.com)
  • The insulin-like growth factor 1 (IGF-1) receptor is a protein found on the surface of human cells. (igf1rsignal.com)
  • of every TAM receptor (gray shading represents lower appearance levels in accordance with various other cell lines). (greatlakeshighereducationnow.com)
  • The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)
  • The members of the L-type amino acid transporter (LAT) family are Na + -independent transporters that deliver neutral amino acids into cells ( 2 ). (spandidos-publications.com)
  • Mutations in the CACNA1S gene change single protein building blocks (amino acids) used to make the CACNA1S protein, which alters the structure and function of calcium channels in skeletal muscle cells. (medlineplus.gov)
  • Here we have explored whether upregulating the low levels of endogenous LFA-1 expression on Jurkat E6.1 cells through transduction with CD11a- and CD18-encoding lentiviruses can improve IS architecture. (ox.ac.uk)
  • The Beilhack group has been investigating immune cell interactions after allogeneic hematopoietic cell transplantation (HCT). (uni-wuerzburg.de)
  • Yet, if tissue clearing is necessary, fixation of the tissues has limited real-time life cell imaging deep within tissues. (uni-wuerzburg.de)
  • Can I use methanol fixation with the 3' Single Cell Gene Expression assay? (10xgenomics.com)
  • If processing fresh samples is not feasible, it may be possible to perform methanol fixation on cell suspensions and to store the methanol-fixed cells for later use with the 3' GEX assay. (10xgenomics.com)
  • However, methanol fixation is harsh and can lead to cell loss and cell lysis, and may not be suitable for all sample types. (10xgenomics.com)
  • The Jurkat E6.1 clone has been extensively used as a powerful tool for the genetic and biochemical dissection of the TCR signaling pathway. (ox.ac.uk)
  • These include members of the Bcl-2 protein family, course, and extent of programmed cell death will aid man- which have both proapoptotic and antiapoptotic activity agement of patients with severe infections. (cdc.gov)
  • Besides new methods for the study of conformational dynamics of biomolecules and protein folding, direct stochastic optical reconstruction microscopy ( d STORM) has been developed that enables super-resolution fluorescence imaging with standard organic fluorophores with a spatial resolution of ≤ 20 nm in the imaging plane in fixed and in living cells (Figure) [8]. (uni-wuerzburg.de)
  • Full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell. (novusbio.com)
  • With Low Cell ChIP, researchers can reduce the number of cells they are studying to improve their understanding of the complexities of protein-DNA interactions to a more limited population of cells. (activemotif.com)
  • Gene term, protein awareness and involving angiogenic elements, chemokines, insulin-like expansion aspect 1 (IGF1) and eosinophilic cells were analyzed. (igf1rsignal.com)
  • Many limited samples used in the Low Cell ChIP Kit will be below the limit of detection for instruments commonly used for DNA quantification (e.g. (activemotif.com)
  • Scheller UMuche JMSterry WLukowsky A Detection of clonal T cells in cutaneous T-cell lymphoma by polymerase chain reaction: comparison of mutation detection enhancement-polyacrylamide gel electrophoresis, temperature gradient gel electrophoresis and fragment analysis of sequencing gels. (jamanetwork.com)
  • Prognostic significance of a polymerase chain reaction-detectable dominant T-cell clone in cutaneous lesions of patients with mycosis fungoides. (jamanetwork.com)
  • The U.S. Food and Drug Administration (FDA) approved two CAR T-cell therapies in 2017, Kymriah developed by Novartis for the treatment of children with acute lymphoblastic leukemia and Yescarta developed by Kite for adults with advanced lymphomas. (nature.com)
  • Chhanabhai MAdomat SAGascoyne RDHorsman DE Clinical utility of heteroduplex analysis of TCR gamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders. (jamanetwork.com)
  • These findings suggest that inter- the apoptotic destruction of lymphocytes and dendritic ventions aimed at reducing the extent of immune cell apop- tosis could improve outcomes for a variety of severe cells could have a particularly adverse effect on disease human infections, including those caused by emerging outcome. (cdc.gov)
  • Advances in multi-photon laser-scanning microscopy, super-resolution microscopy and light-sheet fluorescence microscopy as well as improved fluorescent probes and genetically encoded flurorescent reporter genes offer a multifaceted toolbox to explore complex cellular and molecular processes in live tissues. (uni-wuerzburg.de)
  • The GRK consortium provides an ideal environment to foster interdisciplinary collaboration and highly innovative research to quantitatively elucidate host-pathogen interactions at unprecedented molecular, cellular, microanatomical and functional detail in a unique setting that mimics the onset and progression of infectious diseases in a human tissue environment. (uni-wuerzburg.de)
  • Veelken HWood GSSklar J Molecular staging of cutaneous T-cell lymphoma: evidence for systemic involvement in early disease. (jamanetwork.com)
  • Dietary conjugated linoleic acid reduces rat adipose tissue cell size rather than cell number. (raw-milk-facts.com)
  • Published reports of between one to a few thousand cells in a given experiment are reported. (nature.com)
  • Further, cell lines can change genetically over multiple passages, leading to phenotypic differences among isolates and potentially different experimental results depending on when and with what strain isolate an experiment is conducted. (wikipedia.org)
  • Tested by its ability to induce IgM secretion by CpG/CD40L treated human naive B cells, at a concentration of 500 ng/ml. (reliatech.de)
  • Titanium implants release Ti ions into surrounding tissues [246], which additional results in the secretion of IL-1, TNF-, and RANKL in Jurkat T-cells [247], and could aggravate inflammation. (idhinhibitor.com)
  • cBiTE expression and secretion was detected in supernatants from ICOVIR-15K-cBiTE-infected cells, and the secreted BiTEs bound specifically to both CD3 + and EGFR + cells. (aacrjournals.org)
  • We established dynamic multi-photon laser-scanning microscopy (MPM) that reveals complex cellular processes in real-time within tissues. (uni-wuerzburg.de)
  • Thus, our research focuses on the interactions of the immune system with pathogens, cancer and the tissue environment [3-6]. (uni-wuerzburg.de)
  • To address these complex challenges of a dysfunctional immune system caught between defective tolerance and compromised immunity we employ optical imaging methods, i.e. optical techniques that provide single cell resolution in the context of intact tissues. (uni-wuerzburg.de)
  • Because of the excellent optical properties of GNRs, the combination of surface-modified GNRs, with LBL cell encapsulation, should provide substantial benefits. (springer.com)
  • By comparison, Jurkat cells have been used only scantily for imaging on supported lipid bilayers (SLBs) incorporating laterally mobile TCR and integrin ligands, which allow to study synaptic rearrangements of surface molecules and the fine architecture of the mature IS, likely due to limitations in the assembly of immune synapses with well-defined architecture. (ox.ac.uk)
  • Our results highlight a new role for LFA-1 in the core architecture of the IS that can be exploited to study the spatiotemporal redistribution of surface receptors on SLBs, thereby extending the potential of E6.1 cells and their derivatives for fine-scale imaging studies. (ox.ac.uk)
  • Here, we describe a novel approach for creating co-cultures of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells where both cell types are positioned on the same surface in a price spatial configuration (micropattern). (elsevierpure.com)
  • The assay is ideal if working with limited sample amounts, hard to culture cell lines, or small tissue biopsies. (activemotif.com)
  • The transplantation of cells or tissues has been applied for treating various human diseases (Bhatia et al. (springer.com)
  • 2000 ). The encapsulation of cells has been developed to avoid immune rejection in cell/tissue transplantation. (springer.com)
  • Our 3' Single Cell Gene Expression assay was optimized for use with single cell or single nuclei suspensions prepared from fresh cells/tissues. (10xgenomics.com)
  • Peptide-treated Jurkat cells were associated with increased expression of P21, and P53 genes. (celljournal.org)
  • We show that, while forced LFA-1 expression did not affect TCR recruitment to the IS, E6.1 LFA-1high cells assembled better structured synapses, with a tighter distribution of signaling-competent TCRs at the center of the IS. (ox.ac.uk)
  • The enhancement in early signaling E6.1 LFA-1high cells did not affect expression of the early activation marker CD69 but led to an increase in IL-2 expression. (ox.ac.uk)
  • DNA microarray analysis of epithelial cells retrieved from co-cultures with HIV-1-infected vs. uninfected Jurkat cells revealed that epithelial cells from HIV-infected co-cultures exhibited gene expression patterns consistent with disruption of epithelial barrier formation. (elsevierpure.com)
  • This study examined the post-thaw recovery of Jurkat cells cryopreserved in single osmolyte solutions containing sucrose, glycerol or isoleucine, as well as in a combination of the three osmolytes. (nature.com)
  • Immortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. (wikipedia.org)
  • 3T3 cells - a mouse fibroblast cell line derived from a spontaneous mutation in cultured mouse embryo tissue. (wikipedia.org)
  • Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. (wikipedia.org)
  • Immortalised cell lines have also found uses in biotechnology. (wikipedia.org)
  • There are various immortal cell lines. (wikipedia.org)
  • Some of them are normal cell lines (e.g. derived from stem cells). (wikipedia.org)
  • Immortalised cell lines can also be cloned giving rise to a clonal population which can, in turn, be propagated indefinitely. (wikipedia.org)
  • While immortalised cell lines often originate from a well-known tissue type, they have undergone significant mutations to become immortal. (wikipedia.org)
  • Many cell lines that are widely used for biomedical research have been contaminated and overgrown by other, more aggressive cells. (wikipedia.org)
  • For example, supposed thyroid lines were actually melanoma cells, supposed prostate tissue was actually bladder cancer, and supposed normal uterine cultures were actually breast cancer. (wikipedia.org)
  • There are several methods for generating immortalised cell lines: Isolation from a naturally occurring cancer. (wikipedia.org)
  • There are several examples of immortalised cell lines, each with different properties. (wikipedia.org)
  • Most immortalised cell lines are classified by the cell type they originated from or are most similar to biologically. (wikipedia.org)
  • To this end we used deep sequencing (GRO-seq and PRO-seq) and analyzed nascent mtDNA-encoded RNA transcripts in diverse human cell lines and metazoan organisms. (biorxiv.org)
  • This pausing site correlated with the presence of a bacterial pausing sequence motif, yet the transcription pausing index varied quantitatively among the cell lines. (biorxiv.org)
  • Therefore, cell encapsulation of T lymphocytes might help mitigate this problem and increase the efficiency of immunotherapy in many diseases. (springer.com)
  • An immortalised cell line should not be confused with stem cells, which can also divide indefinitely, but form a normal part of the development of a multicellular organism. (wikipedia.org)
  • This is the original method for generating an immortalised cell line. (wikipedia.org)
  • We confirmed that the newly dominant clonotype was derived from an HSV-specific CD4+ T cell by creation of a synthetic TCR in a Jurkat-based cell line with a NR4A1-mNeonGreen reporter system. (medrxiv.org)
  • The resulting increase in calcium ion concentration within muscle cells stimulates muscles to contract, allowing the body to move. (medlineplus.gov)