• 1.1 This practice covers quantitative methods for the sampling and determination of bacterial endotoxin concentrations in water miscible metalworking fluids (MWF). (astm.org)
  • Materials used in cell manufacturing may interfere with the test and therefore validation of bacterial endotoxin testing (BET) is mandated for each cell product. (brighttalk.com)
  • However, endotoxemia in liver transplant recipients has been associated with graft failure and a high mortality rate ( 4 , 5 ), and in gram-negative bacterial infections, the physiologic effects of endotoxin may persist after adequate antimicrobial drug treatment. (cdc.gov)
  • This new general chapter describes a bacterial endotoxins test (BET) that can be used as an alternative to the classic limulus amoebocyte lysate (LAL)-based methods for the quantification of endotoxins from gram-negative bacteria. (edqm.eu)
  • Both texts have been revised to allow the use of recombinant factor C (rFC) to test for bacterial endotoxins, as described in general chapter 2.6.32 . (edqm.eu)
  • For users, this proposed revision will mean that they can select the test described in 2.6.32 directly when testing pharmaceutical waters, i.e. without a side-by-side comparison against the tests described in general chapter 2.6.14 Bacterial endotoxins . (edqm.eu)
  • Absorption of bacterial endotoxin has been shown to occur after release of the occluded superior mesenteric artery in the rat. (bmj.com)
  • This document describes a protocol for a quantitative detection of Gram negative bacterial endotoxin in nanoparticle preparations using an end-point Limulus Amebocyte Lysate (LAL) assay. (duke.edu)
  • Gram negative bacterial endotoxin catalyzes the activation of proenzyme in the Limulus Amebocyte Lysate. (duke.edu)
  • Bacterial endotoxins and host response : proceedings of the 4th International Congress of Immunology Satellite Workshop held in Paris, France, on 26 July 1980 / M. K. Agarwal, editor. (who.int)
  • This information is important because endotoxin is released during multiplication or bacterial death, causing a series of biological effects that lead to an inflammatory reaction and resorption of mineralized tissues. (bvsalud.org)
  • Thus, the aim of this work is to carry out a review of the literature on the role of bacterial endotoxin in the etiology of periapical lesions, its mechanism of action, and to elucidate molecular mechanisms involved in endotoxin's recognition by the immune system and cell activation. (bvsalud.org)
  • The Bacterial Endotoxin (LPS) has been widely studied. (bvsalud.org)
  • Therefore, the objective of this study is to carry out a review of the literature on the role of bacterial endotoxin in the etiology of periapical lesions, as well as to elucidate molecular mechanisms involved in its recognition by the immune system and in cell activation. (bvsalud.org)
  • Although not all cell cultures will be impacted by the more standard endotoxin level, premium FBS can be a sensible choice for researchers concerned that higher endotoxin levels might cause issues for their specific cultures. (corning.com)
  • A highly purified material is used as a standard endotoxin (RSE/CSE), which has served historically to define endotoxin analytically. (europeanpharmaceuticalreview.com)
  • Control standard endotoxin (ACC cat. (moleculardevices.com)
  • A series of dilutions from the control standard endotoxin solution were prepared in LAL reagent water. (moleculardevices.com)
  • It includes a novel 96 well microplate (GOPLATE™) pre-loaded with the required Control Standard Endotoxin (CSE) concentrations for a standard curve, as well as Positive Product Controls (PPC). (integra-biosciences.com)
  • And 100 microliters of control standard endotoxin, to prepare as many intermediate dilutions as needed, to enable the preparation of a calibration standard with a concentration of one endotoxin unit per milliliter. (jove.com)
  • To prepare a 05 endotoxin unit per milliliter quality control, combines 50 microliter of the one endotoxin unit per milliliter of control standard endotoxin solution, with 950 microliters of LAL grade water. (jove.com)
  • Delta endotoxins (δ-endotoxins) are pore-forming toxins produced by Bacillus thuringiensis species of bacteria. (wikipedia.org)
  • Endotoxins are lipopolysaccharide molecules produced by gram-negative bacteria that can cause toxic reactions in patients and lead to serious complications. (brighttalk.com)
  • Endotoxins, part of the outer membrane of Gram negative bacteria, are a potent inducer of neutrophilic airway inflammation. (bmj.com)
  • Therefore, endotoxins are active for much longer than the lifetime of the bacteria themselves. (bmj.com)
  • Endotoxins are complex lipopolysaccharides that perform important structural and functional roles in the outer membrane of most gram-negative bacteria. (corning.com)
  • However, poorly maintained water purification systems, water storage containers, and associated tubing can harbor significant levels of endotoxin-producing bacteria and should always be tested if endotoxins are suspected or discovered in cell cultures. (corning.com)
  • Endotoxin, or lipopolysaccharide (LPS), is a constituent of the outer leaflet (OL) of the outer membrane (OM) of gram-negative bacteria. (europeanpharmaceuticalreview.com)
  • Found in the cell wall of gram negative bacteria, endotoxin has routinely been detected by the sensitive and specific Limulus Amebocyte Lysate (LAL) assay. (moleculardevices.com)
  • Clinical evaluation of culturable bacteria, endotoxins and lipoteichoic acid in teeth with vital normal pulp tissues. (bvsalud.org)
  • The objective of this study was to investigate the presence of culturable bacteria , endotoxins (LPS) and lipoteichoic acid (LTA) levels in teeth with normal vital pulp (NVP) with intact crowns (IC) and those with coronal restoration (CR) limited to the enamel level. (bvsalud.org)
  • Gram-negative bacteria not only have different factors of virulence and generate products and sub-products that are toxic to apical and periapical tissues, but also contain endotoxin in the outer membrane of their cell wall. (bvsalud.org)
  • In dentistry, many studies using different methodologies in vivo and in vitro have emphasized the importance of Gram-negative anaerobic bacteria and endotoxin in the etiology of chronic periapical lesions. (bvsalud.org)
  • We analyzed bulk samples of MWFs for bacteria, fungi, and endotoxin. (cdc.gov)
  • Bacteria and endotoxin levels in the bulk MWF samples were very low, and no fungi were detected in the samples. (cdc.gov)
  • Inhalation of gram-negative bacteria endotoxin. (cdc.gov)
  • The report concludes that the low-grade chronic, systemic, immune-mediated inflammatory response induced by the bacteria and their endotoxins and the proinflammatory mediators circulating through the blood contributes to various biological processes that are involved in neurological impairment and cerebral ischemia," said Leira, who is one of the report's authors. (medscape.com)
  • Full Title: Validation Of Endotoxin Testing For CAR T-cell Manufacturing Quality Control & Benefits of Cartridge Technology During manufacture of CAR T-cells there is a risk of contamination of the final product and/or materials with endotoxins. (brighttalk.com)
  • This article looks at best practices to reduce the risk of endotoxin contamination. (corning.com)
  • Although much remains unknown about the mechanisms through which endotoxins interact with in vitro cell cultures, it is clear that endotoxin contamination remains a major source of concern. (corning.com)
  • Residual endotoxin contamination in advanced biotherapy products is an expensive and often difficult contaminant to control. (lifescience.ca)
  • The final concentrations used to generate a standard and curve were 50, 5, 0.5, 0.05, and 0.005 EU/mL. 50 µL from each dilution series was transferred in triplicate to the 96-well Pyroplate ® microplate, which is certified free of interfering endotoxin and glucan contamination. (moleculardevices.com)
  • Here, we present a case study that describes the framework composed of three different LAL formats to estimate potential endotoxin contamination in nanoparticles. (jove.com)
  • EtEraser HP Endotoxin Removal Kit are designed to remove endotoxin contamination from aqueous solutions. (gaiascience.com.sg)
  • 5.1 The determination of endotoxin concentrations in MWF is a parameter that can be used in decision-making for prudent fluid management practices (fluid draining, cleaning, recharging or biocide dosages). (astm.org)
  • 9 Recent measurements on the endotoxin concentrations in house dust have been summarised by Michel. (bmj.com)
  • High concentrations of inhaled NO attenuated HPV in NOS2-deficient mice challenged with endotoxin. (nih.gov)
  • 2. The present study examines the effect of feeding with fat diets rich (corn oil) and poor (coconut oil) in linoleate at high and low concentrations on responses to endotoxin. (portlandpress.com)
  • The concentrations of airborne dust and endotoxin determined on the examined herb farms were large. (aaem.pl)
  • The concentrations of airborne endotoxin determined during peppermint and chamomile processing were within a wide range 1.53-208.33 µg/m 3 and 0.005-2604.19 µg/m 3 respectively (medians 57.3 µg/m3 and 0.96 µg/m 3 ). (aaem.pl)
  • In conclusion, farmers cultivating peppermint are exposed during processing of this herb to large concentrations of airborne microorganisms, dust and endotoxin posing a risk of work-related respiratory disease. (aaem.pl)
  • Endotoxin concentrations were 329 EU/m or 1122 pmol/m and 13.5 EU/m or 110 pmol/m, respectively, for dairy and control workers. (cdc.gov)
  • Commercial infant cereals contain high concentrations of endotoxins and viable Bacillus spp. (lu.se)
  • Endotoxins can be detected reliably by the limulus amoebocyte lysate (LAL) test. (brighttalk.com)
  • 14 The kinetic limulus amoebocyte lysate (LAL) method, an in vitro biological method, has been widely used for endotoxin measurements since the 1980s. (bmj.com)
  • Differential responses of the endothelial and epithelial barriers of the lung in sheep to Escherichia coli endotoxin. (jci.org)
  • Although intravenous Escherichia coli endotoxin has been used extensively in experimental studies to increase lung endothelial permeability, the effect of E. coli endotoxin on lung epithelial permeability has not been well studied. (jci.org)
  • However, prior challenge with Escherichia coli endotoxin markedly impaired the ability of LMBO to reduce QLPA/QPA in wild-type, but not in NOS2-deficient, mice. (nih.gov)
  • Escherichia coli endotoxin is a potent stimulator of interleukin 1 production from macrophages. (portlandpress.com)
  • Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. (sigmaaldrich.com)
  • Each cartridge is supplied with a predetermined spike recovery with two sample and two spiked channels enabling quick and easy endotoxin detection. (brighttalk.com)
  • In this webinar, you will learn from our experts: Introduction to cartridge technology and its benefits How the Endosafe nexgen-PTS provides a rapid quantitative method for Endotoxin detection in CAR T-cell products. (brighttalk.com)
  • PyroSmart NextGen™ is the result of years of research and development by leading endotoxin detection specialists, Associates of Cape Cod Inc. It follows the same enzymatic cascade pathway as naturally-sourced reagents and can be used as a direct, synthetic replacement. (rssl.com)
  • Endotoxin detection test is one of the most critical quality control tests required by the FDA for all drugs in their final formulation. (genscript.com)
  • ToxinSensor Endotoxin Detection System uses FDA approved LAL (Limulus Amebocyte Lysate) testing methods to achieve a fast and highly sensitive endotoxin assay. (genscript.com)
  • Our unique combination of Endosafe® endotoxin testing, Celsis® rapid microbial detection, and Accugenix® microbial identification and strain typing keeps your manufacturing operations running efficiently and smoothly, lowers your cost to manufacture, and protects your reputation. (rxinsider.com)
  • Detection of endotoxins in engineered nanomaterials represents one of the grand challenges in the field of nanomedicine. (jove.com)
  • Endotoxins are usually measured in samples of airborne or settled dust. (bmj.com)
  • A study of the delta-endotoxin of Bacillus thuringiensis var. (who.int)
  • Endotoxinas producidas por BACILLUS THURINGIENSIS utilizadas en plantas transgénicas e insecticidas. (bvsalud.org)
  • Endotoxins produced by BACILLUS THURINGIENSIS used in transgenic plants and insecticides. (bvsalud.org)
  • In a centrifuge, the membrane pores dilate and the eluate, free of endotoxin, passes into the collection tube. (lifescience.ca)
  • The timing of exposure is not the only potential influence on the long-term effects of endotoxin on changes in lung function. (nih.gov)
  • 1.5 This practice seeks to minimize inter-laboratory variation of endotoxin data but does not ensure uniformity of results. (astm.org)
  • We fit a mixed-effects model to estimate mean levels and the variation of endotoxin between homes, between rooms, and between sampling times. (harvard.edu)
  • Endosafe nexgen-PTS, (Charles River) is a portable system designed to read cartridges loaded with LAL reagents and endotoxin control. (brighttalk.com)
  • For many cultures, this level is suitably low to avoid adverse effects on in vitro cell growth, yet it is important to be aware that any reagents added to media after it has been filtered can also harbor endotoxins. (corning.com)
  • It comes in response to growing industry demand for a reliable and effective alternative to the longstanding practice of using LAL reagents derived from horseshoe crab blood for endotoxin testing. (rssl.com)
  • The object of this study is to find out if an inhalation of endotoxin (LPS) induces a local and systemic inflammation and if yes - how to prove this inflammation. (uni-frankfurt.de)
  • Inhalation of endotoxin is followed by clinical and laboratory signs of systemic infammation, whereas no significant increase of bronchial eNO (exhaled NO) was observed as an indicator of airway infammation. (uni-frankfurt.de)
  • Endotoxin induces nitric oxide (NO) production by NO synthase 2 (NOS2). (nih.gov)
  • Endotoxin induces the expansion of IL-1β-producing uterine DCs and, in response, more uterine ILC2s produce IL-4, IL-5 and IL-13. (elifesciences.org)
  • The Chromogenic LAL Endotoxin Assay kit can quantitatively detect endotoxin in a broad range (0.01-1 EU/ml). (genscript.com)
  • The Gel Clot Endotoxin Assay kit is a fast qualitative test showing a positive or negative result. (genscript.com)
  • Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays. (abcam.com)
  • Low Endotoxin/Azide Free Anti-Mouse LPAM-1 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays. (southernbiotech.com)
  • Low Endotoxin/Azide Free Anti-Human IL-13 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, ELISA, ELISpot, and neutralization assays. (southernbiotech.com)
  • B. thuringiensis encodes many proteins of the delta endotoxin family (InterPro: IPR038979), with some strains encoding multiple types simultaneously. (wikipedia.org)
  • Some insects populations have started to develop resistance towards delta endotoxin, with five resistant species found as of 2013. (wikipedia.org)
  • Therefore, it is essential to remove endotoxin from human and animal parenteral drugs, biological products, and medical devices. (genscript.com)
  • These kits can be used to remove endotoxin from proteins,peptides,antibodies,or even DNA samples. (genscript.com)
  • In contrast, the risk of non-atopic asthma, characterised by neutrophilic response, is enhanced in subjects with higher endotoxin exposure. (bmj.com)
  • ABSTRACT Few data are available from Eastern Mediterranean countries about levels of domestic allergens and endotoxins. (who.int)
  • After endotoxin challenge and LMBO, systemic oxygenation was impaired to a greater extent in wild-type than in NOS2-deficient mice. (nih.gov)
  • Indeed, maternal ILC2s promote fetal growth and protect against fetal mortality upon systemic endotoxin challenge. (elifesciences.org)
  • Our observations suggest that biologically relevant levels of antigens, including endotoxin, may have been transferred to the recipient. (cdc.gov)
  • Dust samples were collected from mattresses and floors of 457 homes in the Syrian city of Aleppo and analysed for antigens and endotoxins. (who.int)
  • A large number of studies in occupational epidemiology has shown that exposure to endotoxins increases the likelihood of organic dust toxic syndrome, chronic bronchitis, and asthma-like syndrome. (bmj.com)
  • In contrast, it has been shown that exposure to endotoxins in the occupational and environmental setting protects from respiratory allergies and sensitisation to allergens. (bmj.com)
  • While some studies indicated an increased risk of asthma after endotoxin exposure, others have shown that endotoxin exposure protects from asthma. (bmj.com)
  • At the workplace, measures should therefore be taken in order to reduce endotoxin exposure. (bmj.com)
  • In occupational settings, endotoxins mainly occur in environments with high exposure to organic dusts. (bmj.com)
  • Exposure from, for example, intensive animal production facilities increases the background exposure to endotoxins in rural areas 11 compared to urban settings. (bmj.com)
  • Endotoxin exposure has been proposed as an environmental determinant of allergen responses in children. (harvard.edu)
  • To better understand the implications of using a single measurement of house dust endotoxin to characterize exposure in the first year of life, we evaluated room-specific within-home and between-home variability in dust endotoxin obtained from 470 households in Boston, Massachusetts. (harvard.edu)
  • Long-term occupational exposure to cotton dust that contains endotoxin is associated with chronic respiratory symptoms and excessive decline in forced expiratory volume in 1 sec (FEV 1 ), but the mechanisms of endotoxin-related chronic airflow obstruction remain unclear. (nih.gov)
  • In the current study, we examined temporal aspects of the exposure-response relationship between airborne endotoxin exposure, longitudinal change in FEV 1 , and respiratory symptoms in a cohort of Chinese cotton textile workers. (nih.gov)
  • We used a generalized estimating equations approach to model FEV 1 level and respiratory symptoms as a function of past exposure (cumulative exposure up to the start of the most recent 5-year survey interval) and cumulative exposure (within the most recent interval) to endotoxins, after adjusting for other covariates. (nih.gov)
  • Past exposure to endotoxin was associated with reduced FEV 1 level among retired cotton workers. (nih.gov)
  • Recent endotoxin exposure was significantly associated with byssinosis, chronic bronchitis, and chronic cough. (nih.gov)
  • However, the long-term exposure-response relationship between endotoxin and change in lung function and respiratory symptoms is not well understood. (nih.gov)
  • A 3-year longitudinal study of pig farmers observed a significant linear association between endotoxin exposure and annual decline in forced expiratory volume in 1 sec (FEV 1 ). (nih.gov)
  • Previous analyses of the present study population, a cohort of Chinese cotton textile workers, evaluated longitudinal change in FEV 1 and the occurrence of chronic respiratory symptoms in relation to cumulative endotoxin exposure. (nih.gov)
  • Whether the excessive decline in lung function and the occurrence of respiratory symptoms are influenced differently by more recent or more remote exposure to endotoxin is unknown. (nih.gov)
  • In the present study, we evaluated the exposure-response relationship between cumulative endotoxin exposure and longitudinal change in FEV 1 and the occurrence of respiratory symptoms in an updated follow-up of a 25-year prospective cohort study of Chinese cotton textile workers. (nih.gov)
  • Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma. (southernbiotech.com)
  • METHODS: Exposure of 205 dairy and 45 control (vegetable processing) workers to particulate matter and endotoxin was monitored. (cdc.gov)
  • 1.3 This practice gives an estimate of the endotoxin concentration in the sampled MWF. (astm.org)
  • The absorbance (A405) can be measured by the plate reader and is proportional to the endotoxin concentration in the sample. (moleculardevices.com)
  • Then using 900 microliters of LAL grade water, and 100 microliters of the one endotoxin unit per milliliter calibration standard, prepare a second calibration standard, at a concentration of 0.1 endotoxin unit per milliliter. (jove.com)
  • Concentration of endotoxin in a sample is in direct proportion with absorbance and is calculated from a standard curve. (duke.edu)
  • ToxinSensor Endotoxin Assay Kits can be widely used in in vitro end-product endotoxin tests, including those for human and animal parenteral drugs, biological products, and medical devices. (genscript.com)
  • On behalf of the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) requests public comments on nominations received for the three in vitro test methods proposed for detecting and quantifying botulinum neurotoxin (BoNT), and at second for an in vitro test method proposed for detecting non-endotoxin pyrogens. (gmp-compliance.org)
  • CONCLUSIONS: Modern California dairy endotoxin exposures and shift length were associated with a mild acute decrease in forced vital capacity. (cdc.gov)
  • The aim of this study was to determine the levels of microorganisms, dust and endotoxin in the air during processing of peppermint ( Mentha piperita ) and chamomile ( Matricaria recutita ) by herb farmers, and to examine the species composition of airborne microflora. (aaem.pl)
  • Next generation Proteus kits combine the quality separation you expect from gravity flow columns with the speed and ease-of-use of spin columns to give you 4-log reductions of endotoxin without compromising protein or antibody yields. (lifescience.ca)
  • The contact time is maximized to ensure maximum endotoxin depletion without losses of the target protein, antibody or domain antibody. (lifescience.ca)
  • Endotoxin is a major contaminant found in biologically active substances. (genscript.com)
  • This kit utilizes chromogenic LAL assay to quantitatively detect endotoxin in a broad range. (genscript.com)
  • In addition, the department is looking for data generated through alternative test methods for identifying non-endotoxin pyrogens, including but not limited to the monocyte activation test (MAT), which was nominated by Biotest AG. (gmp-compliance.org)
  • Data on non-endotoxin pyrogens tested in the rabbit pyrogen test (RPT) are requested for comparison. (gmp-compliance.org)
  • Thus, the alveolar epithelium in sheep is significantly more resistant than the lung endothelium to the injurious effects of E. coli endotoxin. (jci.org)
  • Mean endotoxin levels varied significantly from room to room. (harvard.edu)
  • Endotoxin levels were significantly higher in wool/cotton mattresses and older houses. (who.int)
  • Endotoxin levels were significantly higher in the evisceration area than the dark meat area, although they were not significantly related to reported employee symptoms. (cdc.gov)
  • 5.2 This standard provides a practice for analysts who perform quantitative endotoxin analyses of water-miscible MWF. (astm.org)
  • For efficient removal of endotoxin from samples! (genscript.com)
  • With repeat use of ToxinEraser endotoxin removal resin. (genscript.com)
  • GenScript ToxinSensor is an efficient endotoxin removal tool. (genscript.com)
  • The final endotoxin level can be reduced below 1 EU/mg with repeated use of ToxinSensor Advanced Endotoxin Removal resin. (genscript.com)
  • The proprietary FlowGo technology regulates sample movement through the high-quality affinity resin matrix, increasing both endotoxin removal and protein recovery. (lifescience.ca)
  • Uniquely, we offer flow rate control for endotoxin removal in a centrifuge. (lifescience.ca)
  • EtEraser™ SE Endotoxin Removal Kit utilizes food-grade modified E-poly-L-lysine as a high-affinity ligand for endotoxins. (gaiascience.com.sg)
  • Particulate matter, endotoxin, and worker respiratory health on large Californian dairies. (cdc.gov)
  • Indeed, the previously referenced study showed some 1M amino acid solutions to have endotoxin levels as high as 50ng/mL. (corning.com)
  • Where possible, researchers should ask manufacturers to confirm the endotoxin levels of their products. (corning.com)
  • When in doubt, the media endotoxin levels should be tested by LAL assay both before and after adding any new components. (corning.com)
  • The purpose of this study was to evaluate the usefulness of a newly developed method (Endotoxin Activity Assay (EAA)) to measure the ET activity (EA) in patients under sepsis compared with the prior method of the limulus amebocyte lysate (LAL) assay and explore the association between EA levels and patients' severity. (scirp.org)
  • L. C. Casey, R. A. Balk and R. C. Bone, "Plasma Cytokine and Endotoxin Levels Correlate with Survival in Patients with the Sepsis Syndrome," Annals Internal Medicine, Vol. 119, No. 8, 1993, pp. 771-778. (scirp.org)
  • Adjusting for season, the correlation of endotoxin levels within homes over time was 0.65 for both the bedroom and kitchen and 0.54 for the family room. (harvard.edu)
  • These results suggest that room-to-room and home-to-home differences in endotoxin influence the total variability more than factors affecting endotoxin levels within a room over time. (harvard.edu)
  • The levels of endotoxin in the pinning room were found to be much higher than in any other area of the evisceration or dark meat areas. (cdc.gov)
  • Endotoxin (ET) is a structural molecule of the Gram-negative bacilli extracellular membrance, which activates targeted cells including macrophages and neutrophils, and causes septic shock. (scirp.org)
  • Once activated, the endotoxin binds to the gut epithelium and causes cell lysis by the formation of cation-selective channels, which leads to death. (wikipedia.org)
  • In addition, other sections of this guidance document identify the risk to health and describe measures that, if followed by manufacturers and combined with the general controls, will generally address the risk associated with these endotoxin assays and lead to a timely premarket notification [510(k)] review and clearance. (fda.gov)
  • The efficacy of prophylactic endotracheal administration of superoxide dismutase (SOD) was assessed in an animal model of acute lung injury induced by intratracheal endotoxin in neonatal piglets. (karger.com)
  • We'll present the validation approach for endotoxin testing using Endosafe nexgen-PTS designed to meet Australian regulatory requirements. (brighttalk.com)
  • This endotoxin tolerance (ET) is mediated via epigenetic mechanisms. (uni-muenchen.de)
  • E. coli endotoxin was administered according to one of three protocols: intravenous alone (5-500 micrograms/kg), intravenous (5 micrograms/kg) plus low-dose alveolar endotoxin (10 micrograms/kg), and high-dose alveolar endotoxin alone (50-100 micrograms/kg). (jci.org)
  • Although intravenous E. coli endotoxin produced a marked increase in transvascular protein flux and interstitial pulmonary edema, there was no effect on the clearance of either the vascular (131I-albumin) or the alveolar (125I-albumin) protein tracer across the epithelial barrier. (jci.org)
  • Morphologic studies confirmed that the alveolar epithelial barrier was not injured by either intravenous or alveolar E. coli endotoxin. (jci.org)
  • DIC was induced by intravenous administration of endotoxin, 20 micrograms/kg/hr during 6 hr. (cun.es)
  • Many commercially available protocols are unable to remove endotoxins effectively and are based on non-affinity chromatography methods e.g. (lifescience.ca)
  • The animals received recombinant human SOD (5 or 20 mg/kg intratracheally) 10 min before induction of acute lung injury with intratracheal endotoxin (20 mg/kg). (karger.com)
  • Thus, the present findings suggest that prophylactic treatment with SOD may be, at least in part, effective for alleviating acute lung injury caused by endotoxins. (karger.com)
  • The protocol is based on QCL-1000 kit insert manufactured by BioWhittaker/Cambrex Corporation (10.1) and the US FDA Guideline "Validation of the LAL test as an end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices" (10.2). (duke.edu)
  • 4 The immune stimulatory capacity of endotoxins can only be inactivated at high temperatures (for example, 160°C for 4 hours). (bmj.com)
  • The presence of endotoxin can cause pyrogenic reactions in host organisms such as endotoxin shock, tissue injury, and even death. (genscript.com)
  • To assess the therapeutic efficacy of agents capable of stimulating the fibrinolytic system, such as tissue plasminogen activator (t-PA) and urokinase (UK) on endotoxin-induced disseminated intravascular coagulation (DIC) in the rabbit. (cun.es)
  • In spite of the marked chemotactic effect of alveolar endotoxin, there was no change in the permeability of the epithelial barrier to the vascular or alveolar protein tracers. (jci.org)
  • This kit is a convenient endotoxin qualitative test based on gelation principle. (genscript.com)
  • The ASSIST PLUS is used to perform an ENDOZYME II GO endotoxin test from bioMérieux, using a pipetting program generated by the VIALAB software. (integra-biosciences.com)
  • Or what does this mean in terms of adherence to endotoxin testing requirements, or why do I even need to test for endotoxins. (rxinsider.com)