Technology nucleic acid hybridization. Medical search. Frequent questions

This is the optimized probe length as it minimizes the melting temperature variation among different probes during probe-target hybridization. (wikipedia.org)
Hybridization between the capture probe and the target DNA is achieved by melting and annealing complementary target DNA sequences to their capture probes located on the microspheres. (wikipedia.org)
The technology involves the preparation of a microarray chip, which consists of a solid substrate, usually glass or silicon, with thousands or millions of immobilized DNA probes. (tutorialspoint.com)
The intensity of fluorescence is measured to quantify the amount of hybridization that has occurred between the probes and the target molecules. (tutorialspoint.com)
The hybridization occurs between the complementary sequences of the probes and the target molecules. (tutorialspoint.com)
Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
My research focuses on the synthesis of modified nucleosides, oligonucleotides, metallated oligonucleotides, and their conjugation to develop hybridization probes. (utu.fi)
Modified nucleosides and nucleic acids, Oligonucleotide chemistry, developing covalently mercurated hybridization probes. (utu.fi)
Various technologies have been developed that allow production of "libraries" of synthetic compounds (e.g., pharmaceutical agents, inhibitors, etc.) or nucleic acid probes, etc. (justia.com)
This value is about 50 to 100 times more sensitive than the level achieved with conventional fluorescencebased molecular beacons, and is 10 to 60 times more sensitive than previously reported for other lanthanidebased hybridization probes. (njit.edu)
These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications. (njit.edu)
The technology utilizes fluorogenic oligonucleotide signaling probes and flow cytometry to detect and isolate individual living cells expressing one or more genes. (genelink.com)
The method is based upon the broadly-applicable principles of fluorescence-resonance energy transfer (FRET) and nucleic acid hybridization using fluorogenic oligonucleotide signaling probes originally reported for in tubo qRT-PCR applications, transfected into living cells. (genelink.com)
Detecting gamma-irradiated SARS-CoV-2 virions in an extraction buffer, the current amplified HCR lateral flow assay achieves a limit of detection of 200 copies/µL using nucleic acid probes to target the SARS-CoV-2 RNA genome. (caltech.edu)
By swapping out nucleic acid probes to target different pathogens, amplified HCR lateral flow assays offer a platform for adaptable and sensitive at-home testing for emergent diseases. (caltech.edu)
Development of commercially available molecular methods, such as nucleic acid hybridization probes, improved turnaround times significantly, but can lack specificity. (bruker.com)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
In ECHO probes, a hybridization-dependent fluorescent nucleotide regulated by the H-aggregation of thiazole orange organic dyes (D 514 ) is incorporated into specific sequence contexts and serves as fluorescent detection readout for target nucleic acids. (ne.jp)
The target of ECHO probes is only the complementary single-stranded nucleic acid. (ne.jp)
If you have the data on the secondary structure of the target nucleic acid, it would give a hint for the design of probes. (ne.jp)
The development of new probe chemistries, such as hybridization probes and locked nucleic acid probes, has improved the sensitivity and specificity of real-time PCR assays. (kingsresearch.com)
Here, we describe a microfluidic-based method which enables the detection of microRNAs or miRNAs in single intact cells by flow-FISH using locked nucleic acid (LNA)-containing probes. (nih.gov)
Following appropriate sample preparation, the next step in solving the puzzle is to provide sufficient access for in situ hybridization probes to their targets. (bio-techne.com)
The proprietary formulations of these reagents have been optimized to provide enhanced access of in situ hybridization probes to nucleic acid targets across a wide variety of tissue sample types including FFPE, fresh‑frozen (FF), and fixed frozen tissue as well as tissue microarray (TMA) and cultured adherent and non-adherent cells. (bio-techne.com)

Lateral flow assays and electrochemical test strips are the more traditional methods that currently dominate the market," says Luyun Jiang, PhD, technology analyst for market research firm IDTechEx. (medicaldesignbriefs.com)
Recurrent use of assays & kits in the detection of various diseases through molecular diagnostics, rise in product approvals, and technological advancements in molecular techniques contribute to the large market share of this segment. (meticulousresearch.com)
Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
xTAG ® Technology uses a proprietary universal tag system that allows easy development and optimization of nucleic acid assays. (diasorin.com)
Sequences have been optimized to minimize cross-hybridization, thus preventing cross-talk in multiplexed nucleic acid assays. (diasorin.com)
Product manuals and relevant tech notes containing detailed sample prep advice for individual assays are found in the Product Documents section of Support . (bio-techne.com)
Product manuals and relevant tech notes containing detailed pretreatment protocols for individual assays are found in the Product Documents section of Support. (bio-techne.com)

While older, PCR continues to be innovated and remains a central tool in molecular diagnostics laboratories of all kinds, and in situ hybridization. (kaloramainformation.com)
PNA-FISH (peptide nucleic acid fluorescence in situ hybridization) testing on a positive blood Cx: rapid detection of C. albicans and C. glabrata . (hopkinsguides.com)
Flow cytometry in combination with fluorescent in situ hybridization (flow-FISH) is a powerful technique that can be utilized to rapidly detect nucleic acids at single-cell resolution without the need for homogenization or nucleic acid extraction. (nih.gov)
Similar to solving a jigsaw puzzle, successfully performing the technique of in situ hybridization (ISH) benefits from a systematic, step-wise approach that concentrates on one stage of the process at a time, then moves onto the next. (bio-techne.com)
The ACD in situ hybridization kits (RNAscope, BaseScope™, miRNAscope™, and DNAscope™) can be used with formalin-fixed, paraffin-embedded (FFPE) samples, fresh-frozen (FF) or fixed frozen tissues, and both adherent and non-adherent cultured cells. (bio-techne.com)

Policy statement: automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF system. (bvsalud.org)

The direct DNA hybridization approach is the simplest suspension array assay whereby 15 to 20 bp DNA oligonucleotides attached to microspheres are amplified using PCR. (wikipedia.org)
This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
The detection of IgY in yolk samples by ELISA using streptavidin-biotin binding as part of the assay methodology requires some technology to neutralize high concentration of biotin on sample or more steps beyond delipidation to isolate the target protein. (preprints.org)
Here, we enhance sensitivity by developing an amplified lateral flow assay that incorporates isothermal, enzyme-free signal amplification based on the mechanism of hybridization chain reaction (HCR). (caltech.edu)
In addition to providing customers with luminex multiplex assay, we can also provide enzyme-linked immunosorbent assay (ELISA), flow cytometry (FACS analysis) technology to meet other customer needs. (creative-proteomics.com)
FIND) has partnered with Cepheid, Inc. (Sunnyvale, CA) and the University of Medicine and Dentistry of New Jersey (UMDNJ, Newark, NY) to develop a TB-specific automated, cartridge-based nucleic amplification assay (Xpert MTB/RIF) based on the GeneXpert multi-disease platform, currently unique in its simplification of molecular testing with fully integrated and automated sample preparation, amplification and detection required for real-time polymerase chain reaction. (bvsalud.org)
2001. Evaluation of benzene exposure in children living in Campania (Italy) by urinary trans,trans -muconic acid assay. (cdc.gov)

The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are immobilized on a solid substrate. (tutorialspoint.com)
The principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid molecules. (tutorialspoint.com)
The technique consists in separating nucleic acids from complex mixtures by complementary hybridization. (biotrend.com)
Another crucial aspect of DNA nanotechnology is the hybridization of complementary chemical nucleic acids. (tatayoung.com)
The ratio of quantum yields between after and before addition of the complementary nucleic acid strands showed a strong relationship with the number of possible Watson-Crick base pairs formed within two bases from D 514 in the 'self-dimer' of the probe. (ne.jp)

Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. (njit.edu)
The clear change in fluorescence intensity depending on hybridization is useful for visible gene analysis. (ne.jp)
Using laser detection technology, qualitative and quantitative analysis of microsphere encoding and reporting fluorescence. (creative-proteomics.com)

The advent of the nucleic acid labeling technique, coupled with the development of genomics, has significantly increased the use of molecular biology enzymes, kits, and reagents for the detection of pathogenic events at the genomic level. (technavio.com)

Stata DX licensed the eRapid portable electrochemical sensing technology to develop diagnostics for neurological, cardiovascular, and renal diseases with a first focus on building a point-of-care platform for difficult-to-detect neurological disorders. (harvard.edu)
Lab-on-a-chip and molecular diagnostics are the emerging new technologies. (medicaldesignbriefs.com)
Molecular diagnostics are used to asses an individual's health condition at the molecular level, analyzing specific genetic sequences in DNA, ribonucleic acid (RNA), or the material or protein that is encoded in these genetic sequences, says Jiang. (medicaldesignbriefs.com)
In recent decades, the introduction of various new genetic technologies for research use and diagnostics has revolutionized many areas and opened new frontiers in healthcare. (kaloramainformation.com)
The growth of this market is driven by technological advancements in molecular diagnostics, the increasing use of PoC diagnostic tests, an increase in funding for the R&D of diagnostic products, the rising geriatric population, and the increasing prevalence of target diseases. (meticulousresearch.com)
Among the technologies included in the report, in 2023, the polymerase chain reaction (PCR) segment is expected to account for the largest share of the Europe molecular diagnostics market. (meticulousresearch.com)
The frequent and repetitive use of diagnostic kits, the large portfolio of disease-specific kits for the early diagnosis of chronic and infectious diseases, and technological advancements in molecular diagnostics support the large market share of this segment. (meticulousresearch.com)
He holds strong expertise in molecular technologies such as Polymerase Chain Reaction (PCR)-Amplicor viral load quantification for human immunodeficiency virus (HIV) (Roche Diagnostics), branch DNA (bDNA) System 440 (Siemens Diagnostics) viral load for HIV and Hepatitis C Virus (HCV). (sivams.com)
In addition to the growing demand for dPCR technology in scientific applications, there is also a growing interest in the use of dPCR in clinical diagnostics. (kingsresearch.com)

Her professional interests include protein structure and function, catalytic mechanisms of enzymatic reactions, sequencing technologies, purification technologies, and all things microbiology and marine biology. (pcrassays.com)

Relative to proteins, complex nucleic acid-based systems can be designed more readily due to the countable nature of base-pairing interactions and readily available physical models. (caltech.edu)
Not only must cell membranes be permeabilized for best results, but the crosslinking of nucleic acids to proteins that occurs during preparation of fixed samples can mask targets and must be relieved. (bio-techne.com)
Structural and functional interaction of nucleic acids and proteins in the replication, transcription and translation of genetic material. (charlotte.edu)

TAG technology constitutes the proprietary sequences designed by Luminex for nucleic acid applications. (diasorin.com)
Only three bases are used (TAG), preventing hybridization with native sequences. (diasorin.com)

In addition to the advantages of real-time PCR in the diagnosis and monitoring of infectious illnesses , this technology is also used in the study of molecular epidemiology of infectious diseases. (kingsresearch.com)
In recent years, end-users such as hospitals, diagnostic centers, academic institutes, and research organizations have been increasingly opting for nucleic acid labeling techniques for disease diagnosis. (technavio.com)
10 Instead, both PCR and improved culture technology accelerated diagnosis and treatment. (mlo-online.com)

Based on the famous ""mfold"", the UNAFold software package is an integrated collection of programs that simulate nucleic acid folding and hybridization, and its melting pathways for one or two single-stranded molecules. (rpi.edu)

Nucleic acid labeling is widely used by end-users for the detection of molecular infectious diseases. (technavio.com)
Such increasing use of nucleic acid labeling for exploring disease-related genes and management of infectious diseases is expected to positively impact the growth of the global nucleic acid labeling market. (technavio.com)

In particular, these compounds can be used for labeling nucleic acids. (justia.com)
This nucleic acid labeling market research report provides valuable insights on the post COVID-19 impact on the market, which will help companies evaluate their business approaches. (technavio.com)
What will the Nucleic Acid Labeling Market Size be During the Forecast Period? (technavio.com)
The increasing research and development (R&D) activities at academic institutes and medical organizations is notably driving the nucleic acid labeling market growth, although factors such as a lack of skilled workforce may impede the market growth. (technavio.com)
Our research analysts have studied the historical data and deduced the key market drivers and the COVID-19 pandemic impact on the nucleic acid labeling industry. (technavio.com)
One of the key factors driving growth in the nucleic acid labeling market is the increasing research and development (R&D) activities at academic institutes and medical organizations. (technavio.com)
Such research studies on genome sequencing and genetics, in turn, increase the adoption of nucleic acid labeling by research laboratories. (technavio.com)
These institutes make use of nucleic acid labeling techniques to conduct their R&D activities. (technavio.com)
Such factors are thus expected to propel the growth of the global nucleic acid labeling market. (technavio.com)
The increasing investment in the development of new biotechnological techniques is a nucleic acid labeling market trend that is expected to have a positive impact in the coming years. (technavio.com)
The lack of a skilled workforce will be a major challenge for the nucleic acid labeling market during the forecast period. (technavio.com)
Nucleic acid labeling needs skilled professionals such as pathologists and clinicians who can perform nucleic acid labeling tests, genotyping tests, and predisposition diagnostic tests. (technavio.com)
The lack of adequate training and courses on nucleic acid labeling tests available for the detection and staging of a disease is one of the prime factors limiting the number of students graduating to become laboratory technicians. (technavio.com)

NUPACK is a growing software suite for the analysis and design of nucleic acid structures, devices, and systems serving the needs of researchers in the fields of nucleic acid nanotechnology, molecular programming, synthetic biology, and across the life sciences. (caltech.edu)

Conventional laboratory methods are slow and cumbersome and novel technologies for rapid detection are therefore the focus of TB research and development. (bvsalud.org)

The startup aims to leverage the Wyss-developed eRNA technology for controlled enzymatic RNA oligonucleotide synthesis to bring a versatile, sustainable, and scalable RNA synthesis technologies to market to unlock the development of new RNA drugs, vaccines, and gene-editing therapies. (harvard.edu)
These conjugates can be used as substrates for various kinds of polymerizing enzymes in the enzymatic synthesis of nucleic acids. (justia.com)

xTAG ® Technology combined with Luminex instruments is a complete solution for mid-density arrays evaluating gene expression, genotypes, or miRNA. (diasorin.com)

The principle of DNA microarray is based on the hybridization of DNA strands. (tutorialspoint.com)

Diverse Loci cation of nucleic acid amplifications requires selecting appro- priate oligonucleotide primers and optimizing conditions to Associated with maximize sensitivity and specificity. (cdc.gov)

Where my research was focused on the biomimetic total Synthesis of complex natural products mainly bioactive lactones from carbohydrate scaffolds and Artemisinic acid glycoconjugates synthesis. (utu.fi)
I later moved to the University at Albany, State University of New York (SUNY), USA for my postdoctoral research, where I worked on α-functionalization of Cysteine type amino acids with various alkyl halides using photocatalysis and dimeric compounds synthesis through dearomatization reactions. (utu.fi)

Nucleic acid nanotechnologies have provided a platform where biologically relevant molecules can be engineered to perform programmable functions. (caltech.edu)
In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)

The recent emergence of advanced mass spectrometry (MS) technology, specifically Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) MS, enables clinical microbiology laboratories to master the challenge of mycobacteria identification. (bruker.com)

Although current libraries are valuable, commercially-available resources useful in the search to identify new compounds with desirable activities, there are practical difficulties (e.g., cost, time, equipment, etc.) associated with the preparation, storage, and use of current technologies. (justia.com)

Reaction To Detect hybridizations or polymerase chain reaction (PCR), apply a single detection method to a diversity of organisms. (cdc.gov)
Real-time polymerase chain reaction (PCR) technology has become a prominent tool in many molecular biology applications, including pathogen detection, gene expression analysis, and drug development, which propels the market expansion. (kingsresearch.com)
Polymerase Chain Reaction [PCR]) or hybridization with a nucleic acid probe. (cdc.gov)

ACD's RNAscope ISH Technology kits represent a huge advance in the power and ease of use of ISH, greatly simplifying and streamlining time-tested methods while routinely delivering single-molecule detection levels of sensitivity. (bio-techne.com)
Proper preparation of samples is the foundation for achieving the high levels of sensitivity and specificity possible with RNAscope ISH technology. (bio-techne.com)

These features of nucleic acids enable us to design novel interaction pathways and functions by providing well-behaved molecular mechanisms. (caltech.edu)

AcousticaBio is using its proprietary fluid processing technology to transform antibody infusions from an in-hospital IV treatment to a painless subcutaneous injection that can be administered anywhere, improving the clinical care of millions of patients. (harvard.edu)
3EO Health startup uses the Wyss Institute's user-friendly diagnostic nucleic acid detection technology together with additional components to build a digital platform that may empower patients with self-testing capabilities and access to results, information, and clinical partners. (harvard.edu)
Although qPCR technology has traditionally been used in research, clinical laboratories are increasingly using it for diagnostic purposes. (kingsresearch.com)
A person with laboratory isolation of typical gram-negative, oxidase-positive diplococcic by culture (presumptive Neisseria gonorrhoeae ) from a clinical specimen, or demonstration of N. gonorrhoeae in a clinical specimen by detection of antigen or detection of nucleic acid via nucleic acid amplification (e.g. (cdc.gov)

For example, the commercially available microsphere arrays from Luminex xMAP technology uses a 10X10 element array. (wikipedia.org)

Next generation sequencing (NGS) of nucleic acid extracts from plants infected with unknown viruses is expected to yield information about the genomes of previously uncharacterized viruses without any background information on what viruses might be infecting the plant. (usda.gov)
For example, next-generation sequencing (NGS) technologies have become widely used in genetic diseases, and have grown rapidly in oncology, due to the comprehensive insights they can provide at a relatively low cost. (kaloramainformation.com)
Modern molecular biological methods (such as DNA cloning, gel electrophoresis, nucleic acid hybridization, PCR, and DNA sequencing) data analysis and interpretation. (charlotte.edu)

Probe-target hybridization is usually detected by optically labeled targets, which determines the relative abundance of each target in the sample. (wikipedia.org)
Based on Luminex technology platform, Creative Proteomics provides analysis services for key targets of NGF signaling pathway. (creative-proteomics.com)

Biosensor technology can be used in cheap, disposable point-of-care devices, or it can be used to provide continuous monitoring in implantable devices. (medicaldesignbriefs.com)

In the presence of target sequence, hybridization of the sequence-specific probe results in a fluorogenic conformational change. (genelink.com)
The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)

In order to build the devices, researchers have used self assembly and hybridization methods. (tatayoung.com)

[ 6 ] Both technologies use hybridization of labeled nucleic acid transcripts to measure the gene expression. (medscape.com)

Sol Spiegelman was a pioneering molecular biologist whose discoveries accelerated the study of gene action and laid the foundations of recombinant DNA technology. (nih.gov)
A laboratory- oriented course designed to integrate plant molecular biology, recombinant DNA technology, and plant cell and tissue culture. (charlotte.edu)

Microspheres can be sorted based using both their unique optical properties and level of hybridization to the target sequence. (wikipedia.org)
Luminex technology is a multifunctional liquid phase analysis platform, which is developed on the basis of high-speed digital signal processing technology, colored microspheres, applied fluidics and laser technology. (creative-proteomics.com)
The reactions are carried out on microspheres with different fluorescent codes, such as nucleic acid hybridization, antigen-antibody, enzyme-substrate, ligand-receptor binding reactions. (creative-proteomics.com)

Several nucleic acid tests from Luminex are based on xTAG ® Technology. (diasorin.com)
We provide Luminex technology for NGF signaling pathway analysis. (creative-proteomics.com)

Escherichia coli reaction makes this technology more efficient and economical. (cdc.gov)
Two examples of these mechanisms are the conditional guide RNA (cgRNA) and the hybridization chain reaction (HCR). (caltech.edu)
We then used the hybridization chain reaction mechanism to develop an adaptable and sensitive test for the detection of SARS-CoV-2. (caltech.edu)

Developed at the Wyss Institute and licensed in 2022, their technology dictates cells' fates in a single step and has been validated for many applications. (harvard.edu)
This technology has allowed researchers to develop functionalized reprogrammable devices for different applications. (tatayoung.com)

In contrast, strong emission is observed when the ECHO probe binds to its target nucleic acid, because the aggregate is dissolved and each dye independently intercalates into the nascent duplex structure. (ne.jp)

Moreover, real-time PCR technology is a rapidly developing field with many emerging developments that are more versatile, and have the potential to revolutionize many areas of molecular biology research. (kingsresearch.com)

Suspension array technology (or SAT) is a high throughput, large-scale, and multiplexed screening platform used in molecular biology. (wikipedia.org)

Origin BACs were first developed in the mid-1990s by Dr. Shizuya and colleagues at the California Institute of Technology. (tutorialspoint.com)
Dissertation (Ph.D.), California Institute of Technology. (caltech.edu)

Anatomy1

Organisms4

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes16

Technology, Industry, Agriculture3

Information Science5

Health Care4

Probes26

Assays7

Situ hybridization5

Amplification Technology1

Assay7

Complementary7

Fluorescence3

Reagents1

Diagnostics9

Purification1

Proteins3

Sequences2

Diagnosis3

Single stranded1

Infectious Diseases2

Labeling13

Nanotechnology1

Cumbersome1

Enzymatic2

MiRNA1

Strands1

Primers1

Synthesis2

Molecules2

Mass Spectrometry1

Compounds1

Polymerase3

Sensitivity2

Pathways1

Clinical4

Commercially available1

Sequencing3

Targets2

Biosensor1

Probe results2

Methods1

Gene expression1

Recombinant DNA techno2

Microspheres3

Luminex2

Reaction3

Applications2

Target1

Rapidly1

Molecular biology1

California Institute2