LiverObserved in the cytosolic fractionHepaticProteinRatsSynaptosomalSolubleCellsProteinsMembraneMeSHSpecificBrainActivitiesMajorModelActivityControlLocalizationFractionationMetabolismSpecific subcellularIsolationTranslocationProteomeCytoplasmicIsolateNucleiGeneticsMitochondrialDistributionBiologicalContentProcedureAnalysisFoundHumanPresenceChemicalMouse
Liver7
- Following the OECD guideline 319B PRIMACYT now offers cryopreserved pools of liver subcellular S9 fractions from Common carp kept under controlled conditions for at least two weeks in continuous flow water. (primacyt.com)
- Subcellular liver fractions are a simple but easy to use model system to study Phase I and Phase II reactions, perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests. (primacyt.com)
- Subcellular liver fractions are a simple model system for the analysis of drug biotransformation reactions. (primacyt.com)
- Sprague Dawley rat liver subcellular fractions are isolated from livers obtained from male or female SD rats. (primacyt.com)
- PRIMACYT offers certified cryopreserved Sprague Dawley liver subcellular fractions (S9 and microsomes) from single and pooled donors. (primacyt.com)
- Metabolism of di-2-ethylhexyl phthalate by subcellular fractions from rainbow trout liver. (aspetjournals.org)
- In agreement with such pattern of desensitization, we have detected (by enzymatic and immunological assays) rapid changes in BARK specific activity in different neonatal rat liver subcellular fractions that take place within the same time frame of BAR uncoupling and sequestration. (jci.org)
Observed in the cytosolic fraction1
- An additional 1.5-fold enhancement was observed in the cytosolic fraction from PB-treated rats. (aspetjournals.org)
Hepatic3
- Hepatic subcellular fractions were prepared, and the protein concentration of each fraction was determined. (cdc.gov)
- In this study, GST activities were detected in the hepatic cytosolic and sonic-disrupted mitoplast fractions isolated from male Sprague-Dawley rats by using 1-chloro-2,4-dinitrobenzene as a substrate. (aspetjournals.org)
- And as the premier supplier of hepatic products, including hepatocytes and subcellular fractions, BioIVT enables scientists to better understand the pharmacokinetics and drug metabolism of newly discovered compounds and their effects on disease processes. (massbio.org)
Protein5
- Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. (elifesciences.org)
- Protein function is critically determined by subcellular localization, as organelles offer different chemical environments and interaction partners. (elifesciences.org)
- In order to regulate protein activity, many biological processes involve changes in protein subcellular localization. (elifesciences.org)
- Protein targeting to specific subcellular compartments is a shared feature of cells, but neurons take the challenge to its apogee. (ru.nl)
- Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. (ru.nl)
Rats1
- Alkyltin Inhibition of ATPase Activities in Tissue Homogenates and Subcellular Fractions from Adult and Neonatal Rats (Journal Version). (epa.gov)
Synaptosomal3
- Both sialoglycoproteins and ganglioside contents were markedly increased in microsomal and synaptosomal fractions and decreased in the mitochondrial fractions although the increase in the synaptosomal fractions has been found to be most striking. (caluniv.ac.in)
- After chronic treatment, both ganglioside and sialoglycoprotein content did not change substantially in all the fractions except for a small increase in case of synaptosomal fractions. (caluniv.ac.in)
- We specifically investigated GRIK2/3 and GRIK5 levels in the subcellular synaptosomal (SYN) fraction using western blot. (helsinki.fi)
Soluble3
- The binding of αSyn to ClpP further promotes a distribution of ClpP from soluble to insoluble cellular fraction in vitro and in vivo, leading to reduced solubility of ClpP. (springer.com)
- In the cell-free reconstitution system, the membrane-bound Suc-Ala-Ala-Ala-MCA-hydrolyzing enzyme from CHO cells was also activated by phorbol ester in the presence of the soluble fraction, ATP, and vanadate. (nih.gov)
- He localized the biologic activity to a fraction of lipid soluble acids that he termed "prostaglandin," hypothesizing that these substances originate in the prostate gland. (medscape.com)
Cells3
- Using the fluorogenic substrate succinyl-Ala-Ala-Ala-4-methylcoumaryl-7-amide (Suc-Ala-Ala-Ala-MCA), containing the sequence similar to the cleavage sites for proTGF-alpha processing, we identified a putative candidate proTGF-alpha-converting enzyme in the membrane fractions of Chinese hamster ovary (CHO) cells. (nih.gov)
- Using the endothelin-1 (ET-1)-stimulated elevation in cGMP in LLC-PK1 cells as a biological detector system for the conversion of big ET-1 (bET-1) to ET-1, we detected bET-1-converting activities in subcellular fractions from bovine aortic cultured endothelial cells (BAE) and rat brain. (aspetjournals.org)
- After a semester at Texas Tech University, USA where I studied atmospheric and geoscience, I worked as upper-secondary teacher for three years before turning back to the university in 2002 to get a master degree in Biophysics, thereby turning from large systems i.e. universe and the atmosphere, towards systems on the micro-nano scale i.e. cells and subcellular structures. (lu.se)
Proteins2
- Loading controls can be general whole cell proteins or specific markers for subcellular fractions (e.g. nucleus, mitochondria, membrane). (novusbio.com)
- The BioChain CNMCS kit was designed for the enrichment of proteins based on its subcellular fraction. (biochain.com)
Membrane1
- Within the rat brain, phosphoramidon-inhibitable conversion of bET-1 to ET-1 was detected principally in the cytoskeletal fraction, i.e., that fraction from the membrane that was not solubilized by detergent treatment. (aspetjournals.org)
MeSH1
- Subcellular Fractions" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
Specific1
- Also, an effect on synaptic KAR subunit abundance might be specific to either pre- or postsynaptic compartment, and thus more difficult to detect in SYN fractions. (helsinki.fi)
Brain1
- The present paper reports the result of studies undertaken to determine the effects of the in vivo administration of Δ9‐THC on the ganglioside and sialoglycoprotein contents of rat brain subcellular fractions. (caluniv.ac.in)
Activities1
- Within the particulate fraction of BAE, we detected two activities, at pH 3.4 and pH 5.4-7.4. (aspetjournals.org)
Major1
- This graph shows the total number of publications written about "Subcellular Fractions" by people in this website by year, and whether "Subcellular Fractions" was a major or minor topic of these publications. (childrensmercy.org)
Model1
- Pooled Fish S9 fractions - a new in-vitro model metabolic System. (primacyt.com)
Activity1
- Phosphoramidon-inhibitable converting activity was also detected in the cytosolic fraction of BAE. (aspetjournals.org)
Control1
- Quantitatively, GSH conjugates formed in the cytosolic fraction were 23-fold that of control. (aspetjournals.org)
Localization8
- Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. (nature.com)
- Additionally, a protein's function can also be regulated in a spatial manner by modulating its subcellular localization. (nature.com)
- However, both methods rely on isobaric tandem mass tag (TMT) labeling for quantifying subcellular protein localization, and they require extensive off-line peptide fractionation and consequently lengthy liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis time to achieve the desired depth on the proteome, thus minimizing throughput. (nature.com)
- Complementary angiogenic factors are therefore regulated by the subcellular localization of a receptor (F2rl1) that governs angiogenesis. (nih.gov)
- Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. (elifesciences.org)
- Protein function is critically determined by subcellular localization, as organelles offer different chemical environments and interaction partners. (elifesciences.org)
- In order to regulate protein activity, many biological processes involve changes in protein subcellular localization. (elifesciences.org)
- PP2A is unique in its structure, composed of three subunits - catalytic C, scaffolding A, and a variable regulatory B subunit from four gene families that control subcellular localization, substrate specificity, and enzyme activity ( 3 ). (frontiersin.org)
Fractionation5
- Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of protein networks in cells, but involves laborious workflows that does not cover the phospho-proteome level. (nature.com)
- Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. (nature.com)
- Plasma membrane-associated PAR was tested by immunocytofluorescence (ICF) and subcellular fractionation in Vero cells while TNKS role in this structure and cell junction assembly was evaluated using specific inhibitors. (peerj.com)
- 11. High Speed Size Sorting of Subcellular Organelles by Flow Field-Flow Fractionation. (nih.gov)
- Using some of these mouse lines as well as newly developed subcellular fractionation protocols, EUROSPIN partners have characterized key disease-relevant protein complexes of synapses and their protein components. (europa.eu)
Metabolism1
- /metabolism Used with organs, cells and subcellular fractions for biochemical changes and metabolism. (nih.gov)
Specific subcellular2
- Protein targeting to specific subcellular compartments is a shared feature of cells, but neurons take the challenge to its apogee. (ru.nl)
- It is suggested that alteration of the content of calmodulin in specific subcellular sites may have a central role in opiate action and addiction via regulation of multiple calmodulin-sensitive biochemical pathways. (aspetjournals.org)
Isolation1
- 14. Isolation of subcellular fractions from the yeast Saccharomyces cerevisiae. (nih.gov)
Translocation1
- Subcellular translocation of a protein is a dynamic regulatory event, and it is therefore essential to incorporate the temporal dimension when studying protein translocation in response to stimuli. (nature.com)
Proteome2
- In recent years, several studies presented the potential of MS-based proteomics to explore the subcellular proteome. (nature.com)
- This is probably due to the complexity in applying these workflows in a high throughput manner, which substantially limits their usefulness to analyze global subcellular proteome dynamics. (nature.com)
Cytoplasmic1
- The activities of cis-benzene dihydrodiol dehydrogenase and catechol 1,2-dioxygenase were detected in only cytoplasmic fractions of cells grown with benzene, suggesting that, like for other species of benzene-catabolizing bacteria, the expression of these enzymes is confined to the cytoplasm of this organism. (envirobiotechjournals.com)
Isolate3
- For this, we developed methods to isolate highly purified subcellular fractions from this Rhodococcus. (envirobiotechjournals.com)
- For example, different cell lysis buffers can be used to isolate different subcellular fractions. (horizondiscovery.com)
- These samples would be pooled and processed by using available technology to isolate virus particles en masse, recover viral nucleic acids, produce amplified shotgun libraries, carry out shotgun sequencing of the mixture of viral genomes, and reconstruct these genomes in silico with the techniques originally developed to sequence the entire human genome from random fragments. (cdc.gov)
Nuclei1
- The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE). (hindawi.com)
Genetics1
- /genetics Used for genetic aspects of subcellular elements. (nih.gov)
Mitochondrial2
- Approximately 70% of the decarboxylase activity was localised in the mitochondrial fraction. (ucl.ac.uk)
- The acute treatment with morphine decreased calmodulin activity in the mitochondrial-synaptosomal P2 fraction of the striatum, midbrain, and thalamus but had no effect on the cerebellum, which contains a low density of opiate receptors. (aspetjournals.org)
Distribution3
- These findings may have implications for the selectivity of drug actions based on the subcellular distribution of their targets. (nih.gov)
- In subgenomic and genomic analyses of subcellular mRNA partitioning, we report an overlapping subcellular distribution of cytosolic/nucleoplasmic and topogenic signal-encoding mRNAs, with mRNAs of both cohorts displaying noncanonical subcellular partitioning patterns. (duke.edu)
- Methods: Rabbit antibodies were raised against peptides derived from rat Sprouty-1 and Sprouty-2 and were used in Western blot analysis to determine Sprouty distribution in subcellular fractions (pellets and supernatant centrifuged at 5,000 and 20,000 g) and bronchoalveolar lavage fluid (BAL) from adult rat lungs or used in immunohistochemistry. (elsevierpure.com)
Biological1
- Hence, there is still a gap of knowledge in how to obtain deep subcellular proteomes without compromising MS-time to enable the application of these techniques to different experimental conditions with multiple biological replicates. (nature.com)
Content2
- Modification of the subfractionation procedure resulted in an increase in purity of fractions and a decrease in variability of 5-HT content. (erowid.org)
- Walton, N.Y., Nagy, A.K. and Treiman, D.M. (1998) Altered Residual ATP Content in Rat Brain Cortex Subcellular Fractions following Status Epilepticus Induced by Lithium and Pilocarpine. (scirp.org)
Procedure2
- Employing a new differential fluorescence labeling procedure, MT/T ratios will be determined in subcellular fractions. (nih.gov)
- With the modified procedure, the administration of LSD produced a significant increase in 5-HT in the nerve-ending fraction prepared from rat whole brain. (erowid.org)
Analysis2
- An extensive analysis of subcellular serotonin (5-HT) compartmentation with and without reserpine was undertaken in order to localize further the effect of LSD on rat brain 5-HT. (erowid.org)
- Results: Western blot analysis revealed a 30-kDa Sprouty-1 band and a 34-kDa Sprouty-2 band in the supernatant and pellet fractions centrifuged at 20,000 g. (elsevierpure.com)
Found1
- Conversion of TRI to CH, TCA, and TCOH was found with medaka hepatic subcellular fractions. (cdc.gov)
Presence2
- The presence of fatty acyl-CoA elongation (FACE) activity was not detected in any subcellular fraction but an appreciable amount of malonyl-CoA decarboxylase activity was. (ucl.ac.uk)
- We have examined its presence at the subcellular level and characterized this protein on both secretory granule membranes and intragranular matrix. (hal.science)
Chemical1
- The chemical form of cadmium in subcellular fractions following cadmium exposure. (nih.gov)