• The results showed that the T4 treatment on a shelf life of 96 hours still showed a good average value with individual spermatozoa motility values of 45%, spermatozoa viability of 77.3%, spermatozoa abnormalities of 12.5% and semen pH 6.6. (unimor.ac.id)
  • At the end of the study, it was observed that 3% and 4% glycerol containing Tris extender could be used to preserve the post-thaw motility and morphology of cat semen frozen in straws. (tubitak.gov.tr)
  • By contrast, 5 weeks of RF-EME exposure adversely impacted the vitality and motility profiles of mature epididymal spermatozoa. (nature.com)
  • The motility of carp spermatozoa ranged from 80 to 110 sec in different species. (trjfas.org)
  • In particular, increased volumes, motility, activity time and total spermatozoon number and decreased concentration were observed. (tubitak.gov.tr)
  • Falzone N, Huyser C, Fourie F, Toivo T, Leszczynski D, Franken D. In vitro effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential and motility of human spermatozoa. (naturalmedicinejournal.com)
  • Spermatozoa (singular spermatozoon) are specialized gametic cells possessing own motility. (iasri.res.in)
  • Motility of sperm is visible under ordinary microscope in diluted semen sample. (iasri.res.in)
  • Contents Single layer centrifugation (SLC) has been shown to select the most robust spermatozoa from the ejaculate in several species. (slu.se)
  • The main advantage of performing artificial insemination with fresh semen is that one ejaculate can be split among several mares that are ready to be bred, therefore increasing the book of mares for a particular stallion in a given season. (petplace.com)
  • This technique can be used to enable genetic fatherhood to some men who have no spermatozoa in the ejaculate (azoospermia) and in whom spermatozoa cannot be obtained surgically from the testicles. (wikipedia.org)
  • In each of the inseminations, fresh ejaculate was processed in an attempt to remove virus from spermatozoa to avoid HIV-1 transmission. (cdc.gov)
  • In October and December, fresh ejaculate was fractionated by centrifugation through a discontinuous density gradient of polyvinylpyrrolidone-coated silica particles (Percoll((R))**) to separate motile spermatozoa from other cells and seminal plasma. (cdc.gov)
  • Much as they would like to as they get older then fewer men can ejaculate the huge semen loads so they experienced in their youth. (worldwidehealth.com)
  • Semen may also refer to the entire ejaculate, including the sperm used in the fertilization of the female partner's egg. (shadygrovefertility.com)
  • After semen analysis, clearance can be given in case of documented azoospermia and in case of rare nonmotile spermatozoa in the ejaculate at least 3 mo after the procedure. (eur.nl)
  • Semen composition is described in terms of volume of ejaculate (i.e. volume of semen discharged per ejaculate) and sperm concentration (i.e. billions of sperms present in one milliliter of semen). (iasri.res.in)
  • We found evidence that 27 viruses, across a broad range of virus families, can be found in human semen. (cdc.gov)
  • Our search revealed that 27 viruses that can result in viremia have been found in human semen ( Table ). (cdc.gov)
  • During acute infection, ZIKV is detectable in most organs and body fluids including human semen. (springer.com)
  • RF-EMW emitted from cell phones may lead to oxidative stress in human semen. (naturalmedicinejournal.com)
  • WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction. (who.int)
  • Equine frozen semen: freezability and fertility field results. (scielo.br)
  • Semen from 16 Norwegian Red young bulls with unknown fertility was collected and processed after splitting the semen in two aliquots. (sintef.no)
  • In conclusion, this study has showed that immobilized spermatozoa provide equal fertility results as standard processed semen when AI is performed in a blinded field trial, although the immobilization procedure caused increased sperm damage evaluated in vitro compared to standard semen processing procedure. (sintef.no)
  • Generally each milliliter of semen contains millions of spermatozoa (sperm) even but the majority of the volume consists of secretions of the glands into the male reproductive and fertility organs. (worldwidehealth.com)
  • We speculate that keeping the cell phone in a trouser pocket in talk mode may negatively affect spermatozoa and impair male fertility. (naturalmedicinejournal.com)
  • Today, doses of refrigerated stallion semen are stored, sent, and transported in single-use polystyrene transport boxes. (researchgate.net)
  • Toda y, doses of r efrigerated stallion semen are stored, sent, and transported in single-use pol ystyrene transport boxes. (researchgate.net)
  • O uso do sêmen refrigerado e transportado em caixas isotérmicas é uma prática corriqueira na indústria equina em várias partes do mundo, auxiliando na difusão da técnica de inseminação artificial, pois a utilização de sêmen congelado nessa espécie encontra vários fatores limitantes para se tornar rotina ( Loomis, 2006 LOOMIS, P.R. Advanced methods for handling and preparation of stallion semen. (scielo.br)
  • Density gradient centrifugation (in a continuous or discontinuous gradient) can concentrate semen samples with low concentration of sperm, using the density of sperm as a measure of their quality. (wikipedia.org)
  • Spermatozoon concentration (x10^6/ml) was determined by the hemocytometric method. (tubitak.gov.tr)
  • It contains a fluid called seminal plasma and spermatozoa in varying number (sperm concentration). (iasri.res.in)
  • On the other hand, there were no significant differences in semen volume, semen liquefaction time, luteinizing hormone (LH) level in serum, and Cr concentration in both serum and seminal plasma between the exposed workers and the control group. (cdc.gov)
  • However, it is probable that many more viruses capable of causing viremia (presence of virus in the blood) can be found in semen. (cdc.gov)
  • Usual components of blood plasma such as electrolytes , glucose, enzyme, hormones and other organic compounds are also found in semen. (iasri.res.in)
  • For the first time we performed electroejaculation and used computer-assisted semen analysis to provide baseline data on semen quality in a parti-coloured bat ( Vespertilio murinus ). (frontiersin.org)
  • Three RCT's and one Meta-analysis were found in the current review which all showed beneficial effect of omega 3 supplementation on semen parameters at dosages ranging from 500 mg to 2000 mg/day. (nutraingredients-usa.com)
  • ABSTRACT The aims of this prospective, non-comparative study were to determine time to azoospermia and vasectomy success rate based on the results of semen analysis. (who.int)
  • A total of 334 men seeking vasectomy at a clinic in Urmia city, Islamic Republic of Iran were followed bi-weekly up to 24 weeks after vasectomy or until azoospermia was confirmed via semen analysis. (who.int)
  • 1 At this point, it seems prudent for naturopathic physicians and other integrative practitioners to counsel infertile men (diagnosed with semen analysis) to limit the use of cellular phone in talk mode (using bluetooth devices) while phones are in their pockets or clipped to their belts. (naturalmedicinejournal.com)
  • Whether initiating a semen analysis for diagnostic purposes or undergoing treatment, the male partner will need to provide a semen sample to the SGF clinical team. (shadygrovefertility.com)
  • The methods used in this work include western blot, immunohistochemistry,flow cytometry, computer-assisted semen analysis, live imaging of Ca2+ and fluorescence scanning. (conicet.gov.ar)
  • Evidence synthesis: The guidelines discuss indications and contraindications for vasectomy, preoperative patient information and counselling, surgical techniques, postoperative care and subsequent semen analysis, and complications and late consequences. (eur.nl)
  • Preoperative counselling should include alternative methods of contraception, complication and failure rates, and the need for postoperative semen analysis. (eur.nl)
  • The ECOOL Box allows the cooling of semen doses at a rate of-0.13°C/min, with no detrimental effect. (researchgate.net)
  • In total, 2000 semen doses were produced from each bull, divided equally by treatment. (sintef.no)
  • The resultant 'sex-sorted' spermatozoa are then able to be used in conjunction with other assisted reproductive technologies such as artificial insemination or in-vitro fertilization (IVF) to produce offspring of the desired sex - in farming animals but also in human medical practice. (wikipedia.org)
  • Freshly collected semen can then be evaluated and used for artificial insemination of mares. (petplace.com)
  • Artificial insemination programs with fresh semen provide excellent results as long as the semen quality is acceptable and the semen is handled appropriately after collection. (petplace.com)
  • If semen is not handled appropriately and timing of insemination is not accurate, pregnancy rates may be sub-optimal in artificial insemination programs. (petplace.com)
  • To make timing of artificial insemination (AI) relative to ovulation less critical, methods for prolonging shelf life of spermatozoa in vivo after AI have been attempted to be developed. (sintef.no)
  • To investigate the methods used to prepare semen from these men for insemination, semen from five HIV-infected men with hemophilia was processed in the same (Continued on page 255)laboratory using both procedures reported here. (cdc.gov)
  • one reported a couple who had conceived without HIV-1 transmission after insemination with processed semen. (cdc.gov)
  • In addition, assays forbinding to oviductal epithelial cells and IVF were carried out to test the effect of cAMP compared with other known capacitant agents such asheparin (60 μg/ml) and bicarbonate (40 mM).PARTICIPANTS/MATERIALS, SETTING, METHODS: Straws of frozen bovine semen (20?25 × 106 spermatozoa/ml) were kindlyprovided by Las Lilas, CIALE and CIAVT Artificial Insemination Centers. (conicet.gov.ar)
  • Cat semen was extended with 3% and 4% glycerol containing Tris extender and frozen in (0.25 ml) straws. (tubitak.gov.tr)
  • Semen samples chosen as suitable for being frozen were extended 1:1 (semen/extender) by Tris extender containing 20% egg yolk without glycerol [Tris (hydroxymethyl-aminomethane) (2.40 g) - fructose (1.00 g) - citric acid (1.30 g) osmolarity: 285 mOsmol/kg] at +26 ºC. (tubitak.gov.tr)
  • In this study, standard processed semen with the Biladyl extender (control) was compared with semen processed by sperm immobilization technology developed by SpermVital AS in a blind field trial. (sintef.no)
  • These aliquots were processed with the standard Biladyl extender or the SpermVital extender to a final number of 12 × 106 and 25 × 106 spermatozoa/dose, respectively. (sintef.no)
  • 1 Central Institute of Freshwater Aquaculture (I.C.A.R), Aquaculture Production and Environment Division, Cryobiology Laboratory, Kausalyaganga, Bhubaneswar-751002, Orissa, India Viewed : 3539 - Downloaded : 9605 The aim of the study was to investigate the physical difference in biochemical characteristics of spermatological and seminal plasma and also ultrastructure of spermatozoa in six carp species, viz. (trjfas.org)
  • In August, the semen was centrifuged to separate cells from seminal plasma. (cdc.gov)
  • To counteract the negative effects of excess ROS, spermatozoa and seminal plasma have several mechanisms to neutralize free radicals. (scialert.net)
  • To counteract oxidative damage, spermatozoa and seminal plasma have several mechanisms to neutralize free radicals. (scialert.net)
  • Semen is the term given to secretion of male genital tract and it contains spermatozoa and seminal plasma. (iasri.res.in)
  • Semen was filled into 0.25 ml straws and frozen in liquid nitrogen vapor. (tubitak.gov.tr)
  • Frozen semen samples were stored in liquid nitrogen at -196 ºC. (tubitak.gov.tr)
  • Electroejaculation, a method commonly used for semen collection in exotic animals, generally results in poor quality samples from gorillas. (vin.com)
  • Therefore, breeding ability is seldom evaluated in traditional routine BSEs for bulls and rams in which semen is typically collected via electroejaculation. (merckvetmanual.com)
  • When collecting semen from ruminants via electroejaculation, the sperm production potential is estimated by measuring scrotal circumference. (merckvetmanual.com)
  • The excessive generation of oxygen free radicals in the form of ROS by abnormal spermatozoa and by contaminating leukocytes has been identified as one of the few defined etiologies for male infertility. (scialert.net)
  • While semen quality is critical for reproductive success, semen-borne pathogens may contribute to reproductive failure. (frontiersin.org)
  • These results suggest that occupational exposure to chromium (VI) leads to alteration of semen status and may affect the reproductive success of exposed workers. (cdc.gov)
  • The effect of bull was significant on the proportions of dead, superoxide-positive spermatozoa and live, hydrogen peroxide-negative spermatozoa, as well as on membrane integrity, but it was not significant for mitochondrial membrane potential or chromatin integrity. (slu.se)
  • The review concludes that there is a general lack of interventional studies investigating the impact of poor dietary patterns on semen parameters, presumably because it is not ethically justifiable to cause harm to participants through adherence to an unhealthy diet. (nutraingredients-usa.com)
  • Semen from 8 bulls was layered on top of a species-specific colloid, Bovicoll. (slu.se)
  • Goupil S, Maréchal L, El Hajj H, Tremblay M-È, Richard FJ, Leclerc P (2016) Identification and Localization of the Cyclic Nucleotide Phosphodiesterase 10A in Bovine Testis and Mature Spermatozoa. (plos.org)
  • Manual de laboratorio de la OMS para el examen del semen humano y de la interacci'on entre el semen y el moco cervical / traducci'on efectuada por Jorge A. Blaquier. (who.int)
  • As the spermatozoa pass through the flow cytometer in single file, each spermatozoon is encased by a single droplet of fluid and assigned an electric charge corresponding to its chromosome status (e.g. (wikipedia.org)
  • Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre-Seed, FemGlide, Astroglide, and Replens lubricants. (nih.gov)
  • In mammals, numerous viruses are detectable in semen, including viruses that can cause disease in humans, such as Japanese encephalitis virus, foot and mouth disease virus, parainfluenza virus, and paravaccinia virus ( 6 ). (cdc.gov)
  • Several other viruses that result in viremia can cause orchitis and have been detected in human testes, suggesting the possibility that these viruses may also be detectable in semen. (cdc.gov)
  • These 27 viruses come from diverse families, suggesting that the presence of many viruses in semen is unlikely to be exclusively dependent on specific or conserved viral epitopes, ability of virus to replicate within the male reproductive tract, or common mechanisms of immune evasion. (cdc.gov)
  • Other factors that may also influence whether viruses exist in semen are level of viremia, inflammatory mediators (altering blood-barrier permeability), systemic immunosuppression, male reproductive tract immune responses, presence of sexually transmitted diseases, and virus structural stability. (cdc.gov)
  • The objective of this study was to identify the PDE10A isoforms expressed in the testis and germ cells, and to determine the presence and localization of PDE10A in mature spermatozoa. (plos.org)
  • Presence of microfilariae may be expected to decrease semen quality and transmission via this route may challenge the success of reproductive events in females after mating. (frontiersin.org)
  • The evaluation of breeding ability and libido is only partially possible when collecting semen via artificial vagina or manual stimulation in the presence of a female in estrus. (merckvetmanual.com)
  • Men with low semen production volume are hard to treat so there are many products available in the market, which are made for men to increase their natural semen production volume and sperm count. (worldwidehealth.com)
  • Men can decide among these products, which is best to increase sperm count as well as semen production volume. (worldwidehealth.com)
  • However, choosing herbal products for increasing sperm count and semen production volume proves more useful than other chemical products, as the side effects are less. (worldwidehealth.com)
  • When the correlations between obtained values in all age groups were estimated, it was found that there was a positive relation between body weight and length with semen volume. (tubitak.gov.tr)
  • In addition, it was observed that there was a positive relation between body weight, length and semen volume with activity time (p (tubitak.gov.tr)
  • The sperm-rich fluid that the testicle contributes to semen is actually only about 5% of the overall volume that is normally ejaculated. (shadygrovefertility.com)
  • On volume basis, sperms constitute just 1% of semen volume. (iasri.res.in)
  • Other methods of semen collection are available, including collection of semen with the stallion standing on the ground or manual stimulation instead of an artificial vagina. (petplace.com)
  • These are good alternative methods in some cases, particularly with special needs stallions, but have not been used widely for routine semen collection and require special training. (petplace.com)
  • The results indicate that the level of acrosome-intact live spermatozoa in the AI dose did not affect the 56-day NRR for the two semen processing methods. (sintef.no)
  • Following the binding of these antibodies, spermatozoa which undergo apoptosis are sorted by applying magnetic field to the sperm suspension. (wikipedia.org)
  • To select spermatozoa with low DNA damage index the population of sperm could be enriched with spermatozoa with non-fragmented DNA, with techniques like electrophoresis, Z method and MACS (Magnetic Activating Cell Sorting), which in combination with density gradient centrifugation in single sperm preparation protocols results in spermatozoa with superior quality. (wikipedia.org)
  • This study aims to determine the quality of Bali cattle spermatozoa diluted using sugarcane juice-egg yolk. (unimor.ac.id)
  • Omaha's Henry Doorly Zoo instituted a training program with a bachelor group of three gorillas (ages 10, 12, and 12 yr) to determine if semen quality would improve when collected in a noninvasive, nonstressful manner. (vin.com)
  • These organs work together to produce and excrete semen ("seed" in Latin), which is composed of mature spermatozoa residing in a thickened, mucous fluid. (medscape.com)
  • The flow cytometric results revealed a significant higher level of acrosome-intact live spermatozoa in Biladyl-processed semen compared to SpermVital semen. (sintef.no)
  • Although many of the semen enhancers advertise increases in semen production of '100% or more than it', the truth is that only a handful of natural semen supplements and semen product will provide these types of dramatic results. (worldwidehealth.com)
  • This condition is called non-obstructive or secretory azoospermia, as opposed to obstructive azoospermia, in which complete sperm production does occur in the testicles, and potentially fertilizing spermatozoa can be obtained by testicular sperm extraction (TESE) and used for ICSI. (wikipedia.org)
  • the pattern of reactive oxygen species production may be different in SLC-selected spermatozoa compared to unselected samples. (slu.se)
  • These spermatozoa also experienced increased mitochondrial generation of reactive oxygen species after 1 week of exposure, with elevated DNA oxidation and fragmentation across all exposure periods. (nature.com)
  • The male reproductive system consists of a pair of testes that produce sperm (or spermatozoa ), ducts that transport the sperm to the penis and glands that add secretions to the sperm to make semen (see diagram 13.2). (wikibooks.org)
  • Along this route, the genital ducts and accessory glandular structures produce mucous secretions that combine with the spermatozoa to create semen. (medscape.com)
  • The fraction containing motile spermatozoa was washed twice in buffer and introduced into the woman's uterus through a catheter. (cdc.gov)
  • This cited published article, along with other peer-reviewed articles, has elucidated the deleterious effects of talk-mode cellular phone waves on semen vitality beyond mere association but rather via cause and effect. (naturalmedicinejournal.com)
  • This manuscript provides an updated overview of the cellular and molecular mechanisms of ZIKV infection and reviews data on ZIKV persistence in semen and associated risks to the male reproductive system described in human and animal models studies. (springer.com)
  • Even though ejaculation of semen accompanies orgasm and relational enjoyment erection and orgasm are both controlled by separate mechanisms and semen emission is not critical for amusing in most people. (worldwidehealth.com)
  • The semen samples were left under equilibration for 30 min after glycerolization. (tubitak.gov.tr)
  • In four of the five semen samples, leukocytes were present before processing. (cdc.gov)
  • Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre-Seed and FemGlide lubricants. (nih.gov)
  • This study was limited by the fact that it is a pilot study-a small number of participants and nonrandomized to a group of RF EMW‒unexposed men (the in vitro samples of semen were controlled. (naturalmedicinejournal.com)
  • While the standard filarial life cycle pattern involves a vertebrate definitive host and an invertebrate vector, represented by a blood-sucking ectoparasite, our finding suggests that microfilariae of this nematode species may also be semen-borne, with transmission intensity promoted by the polygynous mating system of vespertilionid bats in which an infected male mates with many females during the autumn swarming. (frontiersin.org)
  • Semen from stallions is usually collected with an artificial vagina with the stallion mounted on a mare in heat or a dummy mount. (petplace.com)
  • Most stallions can be easily trained for semen collection with an artificial vagina provided both the handler and semen collection technician are experienced. (petplace.com)
  • Semen from stallions is most commonly collected with the aid of an artificial vagina while mounting a mare in heat, an ovariectomized mare or a dummy mount. (petplace.com)
  • The choice of artificial vagina to be used depends on the preference and experience of the semen collection technician. (petplace.com)
  • A sterile whirl-pack bag or other type of receptacle (i.e. baby bottle or baby bottle liner) is secured to the distal end of the artificial vagina to collect the semen during ejaculation. (petplace.com)
  • The primary objective was to collect semen in a noninvasive, nonstressful manner. (vin.com)
  • We also reveal using a combination of immunological techniques and proteomics analytical tools that the PDE10A isoform X4 is present in the area of the developing acrosome of spermatids and of the acrosome of mature spermatozoa. (plos.org)
  • It can be concluded that the use of cane juice-egg yolk diluent has a positive impact because it is able to maintain the viability of spermatozoa for 96 hours. (unimor.ac.id)