The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. (absoluteantibody.com)
This antibody has also been used, along with other anti-CD43 MABs, in a Western blot analysis to localize leukosialin epitopes. (absoluteantibody.com)
Describes the original use of this antibody, along with other anti-CD43 MAbs, in FC, WB and plate binding assay studies to determine the dimensions of the extracellular part of the leukosialin molecule, and to establish that the antigenic determinants recognized are largely based on linear protein sequences. (absoluteantibody.com)
Flow cytometry using the Anti-CD43 antibody OX-58 (Ab00586). (absoluteantibody.com)
Immunofluorescence staining of fixed rat splenocytes with anti-CD43 antibody OX-58 (Ab00586). (absoluteantibody.com)
The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody. (absoluteantibody.com)
Independent of antibody secretion B cells secrete proinflammatory cytokines, support the formation of ectopic GCs, and serve as antigen presenting cells. (hindawi.com)
Likewise, antigen/antibody complexes are bound by the FcR of APCs and processed in a unique fashion dependent on the epitope specificity of the bound antibody. (hindawi.com)
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody ( MCA54GA ) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody ( STAR132P ) for detection. (bio-rad-antibodies.com)
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Lymphocyte Activation2
Complement component C3d-antigen complexes can either augment or inhibit B lymphocyte activation and humoral immunity in mice depending on the degree of CD21/CD19 complex engagement. (southernbiotech.com)
c) Effect of antibodies and antigen-specific B cells on antigen uptake. (hindawi.com)
Receptor2
van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. (exbio.cz)
A tumor necrosis factor receptor subtype that has specificity for TUMOR NECROSIS FACTOR ALPHA and LYMPHOTOXIN ALPHA. (childrensmercy.org)
Binds3
Right panel: antigen binds to the BCR of antigen-specific B cells and is internalized. (hindawi.com)
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. (wakehealth.edu)
Finally, the mAb 1B11 binds mouse CD43 only when modified with core 2 O-glycans (31). (geogise.com)
Receptors1
The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (thermofisher.com)
Isotype control1
Figure A. FITC conjugated Mouse anti Rat CD43 ( MCA54FT ) and RPE conjugated Mouse IgG1 isotype control ( MCA1209PE ). (bio-rad-antibodies.com)
CD451
The neoplastic cells in blastic plasmacytoid dendritic cell neoplasm (BPDCN) are typically positive for CD45, HLA-DR, CD43, CD4, CD56, and cutaneous lymphocyte-associated antigen (CLA). (medscape.com)
Cutaneous1
The ligand for the HECA-452 mAb Dinaciclib (SCH 727965) is cutaneous lymphocyte antigen (CLA), which is often used in human samples to identify T cells that can bind to E-selectin and have skin homing potential (28, 29). (geogise.com)
CD473
Antigens, CD47" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
This graph shows the total number of publications written about "Antigens, CD47" by people in this website by year, and whether "Antigens, CD47" was a major or minor topic of these publications. (rush.edu)
Below are the most recent publications written about "Antigens, CD47" by people in Profiles. (rush.edu)
Differentiation2
Antigen-driven differentiation of naive Ig-transgenic B cells in vitro. (ox.ac.uk)
Among the network of neurons, only the astrocytes are bright green, demonstrating the specificity of SBI's human GFAP pGreenFire Differentiation Reporter. (reportergene.com)
Markers2
Differentiating B cells enlarged, clustered, and acquired two plasma cell markers, Syndecan and CD43. (ox.ac.uk)
The pDC markers, CD123 (strong), TCL-1 (90% of cases), CD45RA, blood dendritic cell antigen (BCDA)-2 (expressed in more mature cases), and myxovirus A (MxA) may be detected by flow cytometric immunophenotyping (FCI) and/or immunohistochemical (IHC) staining. (medscape.com)
B2201
B220 + /CD43 + /HSA + ) through fully differentiated B cell stages. (southernbiotech.com)
Concentrations2
B cells are highly efficient APCs in situations of low antigen concentrations. (hindawi.com)
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. (thermofisher.com)
Protein2
EPCR protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. [3] It belongs to the MHC class I / CD1 family of proteins, that is characterized by having a deep groove, that in other proteins in the family (but not in EPCR) is usually used for antigen binding. (wikidoc.org)
The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. (wakehealth.edu)
CD191
Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. (thermofisher.com)
Presentation1
Both secreted autoantibodies and BCR on B cells can modulate the processing and presentation of antigen and thereby affect the nature of presented T-cell determinants. (hindawi.com)
Cells2
Using a large population of naive B cells of defined antigenic specificity permits us to study a primary immune response to an Ag, rather than to less physiologic polyclonal stimuli. (ox.ac.uk)
Likewise B cells function as crucial antigen presenting cells in autoimmune diseases that are traditionally viewed as T cell mediated. (hindawi.com)
Human1
During this time, the perception of CD has changed from a rather uncommon enteropathy to a common multi- organ disease with a strong genetic predisposition that is associated mainly with human leukocyte antigen (HLA)-DQ2 and HLA-DQ8. (vdocuments.net)
Mouse1
Figure B. RPE conjugated Mouse anti Rat CD45Ra ( MCA340PE ) and FITC conjugated Mouse anti Rat CD43 ( MCA54F ). (bio-rad-antibodies.com)
Blood1
LewisY blood antigen or simply BG8, is a blood group antigen that has been identified in studies as a potential marker for differentiating pulmonary adenocarcinoma (PACA) from epithelioid mesothelioma (EM). (neuromics.com)
Function2
Different galectins have distinct binding specificities for binding oligosaccharides depending on the tissue in which they are expressed and the function that they possess. (wikipedia.org)
We are investigating how Pbx and Meis proteins contribute to the specificity of Hox function in development and how disruption of their activities leads to neoplasia. (stanford.edu)
Disease1
More recently, TCF4, which regulates and drives downstream transcriptional programs in BPDCN, appears to be a highly sensitive marker for this disease and amplifies diagnostic specificity along with CDR, CD56, CD123, and TCL1. (medscape.com)