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Origin of replicatSequencesAddgene1982RestrictionXmaIRecombinantConstructsIsolationBacteriaRepositoryMosaic virusAmpRGene replacementTransgeneResistanceIncorporatesInsertionDesignationProteinCircularExpressionFunctionalHighRegionPlease contactSectionTransferProduceLinearFunctionFigureResearchDatabaseCellsInsertCellPlantWorkDomain
Origin of replicat3
  • The ori site, or origin of replication, is derived from the plasmid pMB1. (wikipedia.org)
  • Does gene orientation relative to origin of replication matter on small plasmids? (stackexchange.com)
  • To be able to replicate a plasmid in bacteria, you need a origin of replication (ori). (stackexchange.com)
Sequences3
  • In case of utilization for commercial or industrial purposes, solicitors should be aware that some of the DNA sequences employed in the SEVA plasmids could be subjected to intellectual property restrictions, for which SEVA platform disclaims any carrier liability. (seva-plasmids.com)
  • This vector, designated FZ, incorporates a cloning site in the RNA2 cDNA, permitting insertion of nonviral sequences. (researchsquare.com)
  • Early vectors suffered from limitations such as instability and low yields, but this has been addressed by the genetic modification of vector sequences and by delivering virus vectors into plant cells using Agrobacterium tumefaciens [ 39 , 40 ]. (biomedcentral.com)
Addgene4
  • This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. (addgene.org)
  • This vector is NOT available from Addgene. (addgene.org)
  • Addgene is a nonprofit plasmid repository. (addgene.org)
  • How do you read the gene insert domains on a plasmid from Addgene? (stackexchange.com)
19821
  • 1982). Anatomist of Ti-plasmid-derived binary vectors that may replicate in aswell such as Agrobacterium, and integrate into place genomes still, provided the foundation for place biotechnology. (baxkyardgardener.com)
Restriction4
  • These rare restriction sites are also present on the helper plasmid pUC19Sce. (nau.edu)
  • The replacement vector is engineered to contain few restriction sites to gain greater access to restriction sites within cloned DNA fragments, thus facilitating their genetic manipulation. (nau.edu)
  • A basic plasmid vector map showing the restriction sites. (bitesizebio.com)
  • The presence of restriction sites within the insert makes it challenging to digest and clone into the MCS of the vector. (bitesizebio.com)
XmaI1
  • Your strategy would work, but if possible you might be better off using XmaI rather than SmaI, as the latter is a blunt-end cutting enzyme. (stackexchange.com)
Recombinant3
  • Thus bacteria carrying recombinant plasmids in the MCS cannot hydrolyse X-gal, giving rise to white colonies, which can be distinguished on culture media from non-recombinant cells, which are blue. (wikipedia.org)
  • Ten recombinant VHSVs (rVHSVs) were generated, including two parental rVHSVs, by transfecting fish cells with ten individual full-length plasmid constructs along with supporting plasmids using the established protocol. (biomedcentral.com)
  • Plant viruses are useful expression vectors because they can mount systemic infections allowing large amounts of recombinant protein to be produced rapidly in differentiated plant tissues. (biomedcentral.com)
Constructs1
  • Besides the standardized layout of constructs the SEVA format calls for the unequivocal designation for each of the vectors with the nomenclature explained in detail in that section and the code summarized in the plasmid list section. (seva-plasmids.com)
Isolation2
  • What is the role of glucose in plasmid isolation? (stackexchange.com)
  • This is a common misconception: in fact, in the alkaline lysis method for plasmid isolation, the glucose does not act as an osmotic stabiliser. (stackexchange.com)
Bacteria1
Repository1
  • Contributors to the collection of SEVA vectors must be accredited by the curators of the platform to follow the standards specified above, stored in the SEVA repository and assigned a definitive code prior to public release. (seva-plasmids.com)
Mosaic virus2
  • We describe the development of a new vector based on cowpea severe mosaic virus (CPSMV), a plus-strand RNA virus with its genome divided into two RNA segments, RNA1 and RNA2. (researchsquare.com)
  • Pepino mosaic virus (PepMV) (genus Potexvirus , family Flexiviridae ), a widespread plant virus, is a promising candidate expression vector for plants because of its high level of accumulation in its hosts and the absence of severe infection symptoms. (biomedcentral.com)
AmpR1
  • Only the cells with the plasmid containing the ampicillin resistance (ampR) gene will survive. (wikipedia.org)
Gene replacement1
  • A novel pUC19-based gene replacement vector has been developed. (nau.edu)
Transgene3
  • We found that CP replacement vectors were unable to move systemically and that vectors with duplicated SGPs (even heterologous SGPs) suffered from significant transgene instability. (biomedcentral.com)
  • A stable PepMV vector was generated by expressing the transgene as a CP fusion using the sequence encoding the foot-and-mouth disease virus (FMDV) 2A catalytic peptide to separate them. (biomedcentral.com)
  • All cell lines are tested to be free of any transgene vector insertion by PCR analysis and thus are authentic footprint-free iPSCs. (mbpinc.net)
Resistance2
  • Vector (molecular biology) Blue white screen Antibiotic resistance pBLU Yanisch-Perron, C. (wikipedia.org)
  • The observed distribution of resistance plasmids and β-lactamase genes in several clones indicates a high degree of horizontal transfer. (cdc.gov)
Incorporates1
  • This vector incorporates (i) the counterselectable sacB marker, (ii) a lacZα allele for blue-white screening, (iii) an oriT for conjugation-mediated plasmid transfer and (iv) unique cloning sites for SmaI and the rare-cutting meganuclease I-SceI. (nau.edu)
Insertion1
  • Blunt-end cloning is less efficient than traditional methods, and care should be given to avoid empty vector religation or insertion of fragments in both orientations. (bitesizebio.com)
Designation1
  • The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. (wikipedia.org)
Protein1
  • We explored several strategies for vector development including coat protein (CP) replacement, duplication of the CP subgenomic promoter (SGP) and the creation of a fusion protein using the foot-and-mouth disease virus (FMDV) 2A catalytic peptide. (biomedcentral.com)
Circular1
Expression1
  • We report here the construction of a stable and efficient expression vector for plants based on PepMV. (biomedcentral.com)
Functional1
  • The new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits. (researchsquare.com)
High2
Region1
Please contact1
  • For any suggestions, comments or problems with the database or vectors please contact the collection curator (seva at cnb.csic.es). (seva-plasmids.com)
Section2
  • For the nomenclature of vectors that follow the SEVA standard, see the section below. (seva-plasmids.com)
  • Finally, according to the relationship of the surface normal vector and the vertical section of the workpiece's surface, a depth map is reconstructed to achieve the curvature surface, which can be employed to measure the curvature radius of the cylindrical workpiece's surface. (bvsalud.org)
Transfer1
  • Compact baculovirus transfer vector for expressing proteins using the polyhedrin promoter, for use with the BD BaculoGold™ system. (snapgene.com)
Produce1
  • When transformed with the plasmid for uptake and catabolism of the aldohexuronates, model producer strains were able to grow with and produce from D-galacturonate or D-glucuronate as sole carbon source. (springeropen.com)
Linear4
  • pJAZZ-OC and pJAZZ-OK linear vectors. (bjzblg.com)
  • 15-20 kb PCR amplification products cloned into the pJAZZ® linear vector. (bjzblg.com)
  • Mouse genomic DNA sheared to 6-20 kb and cloned into the pJAZZ linear vector. (bjzblg.com)
  • BigEasy v2.0 Linear Cloning Kits include: Dephosphorylated pJAZZ vector pre-cut at either a SmaI (blunt) or NotI site, CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), Sequencing Primers, Positive Control Insert DNA, DNA Terminator® End Repair Enzyme, BigEasy-TSA Electrocompetent Cells in SOLO packaging (1 transformation per tube), Recovery Medium, Transformation Control DNA, and complete protocols. (bjzblg.com)
Function2
Figure1
  • It would be difficult, if not impossible, to clone these into the multicloning site of any vector (Figure 2). (bitesizebio.com)
Research2
  • Due to its extensive use as a cloning vector in research and industry, pUC19 is frequently used in research as a model plasmid. (wikipedia.org)
  • Learn about the latest plasmid technologies and research tools. (addgene.org)
Database1
  • 3- Adhere to Open Access policy and send your new construct (plasmid and strain) to us for inclusion in the SEVA database, where we will assign a code according the SEVA rules. (seva-plasmids.com)
Cells3
  • pUC19 is one of the most widely used vector molecules as the recombinants, or the cells into which foreign DNA has been introduced, can be easily distinguished from the non-recombinants based on color differences of colonies on growth media. (wikipedia.org)
  • The cells which have taken up the plasmid can be differentiated from cells which have not taken up the plasmid by growing it on media with ampicillin. (wikipedia.org)
  • Furthermore, the transformed cells containing the plasmid with the gene of interest can be distinguished from cells with the plasmid but without the gene of interest, just by looking at the color of the colony they make on agar media supplemented with IPTG and X-gal. (wikipedia.org)
Insert4
  • It is unlike sticky-end cloning, where both the insert and the vector contain single-stranded overhangs that complement each other. (bitesizebio.com)
  • Basically, the blunt-ended insert and linearized vector are mixed with DNA ligase, and the reaction proceeds when the insert, vector, and ligase come together in solution. (bitesizebio.com)
  • The insert is joined to the vector when the ligase catalyzes the formation of a covalent bond between the 3'-hydroxyl group of one base to the 5'-phosphate group of the adjoining base. (bitesizebio.com)
  • The presence of 5' phosphates in the fragments being ligated is critical, as the covalent linkage of 5' phosphates with 3' hydroxyl groups circularizes the vector/insert complex and allows it to be cloned upon bacterial transformation. (bitesizebio.com)
Cell2
  • This plasmid is introduced into a bacterial cell by a process called "transformation", where it can multiply and express itself. (wikipedia.org)
  • This bacterium injects a so-called tumor-inducing (Ti) plasmid in to the place cell, where it integrates in to the genome (Yadav et al. (baxkyardgardener.com)
Plant1
  • An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. (researchsquare.com)
Work1
Domain1
  • Renilla luciferase vector encoding the Gal4 DNA-binding domain fused to an estrogen receptor ligand-binding domain. (snapgene.com)